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INDONESIA
HAYATI Journal of Biosciences
Published by Institut Pertanian Bogor
ISSN : 19783019     EISSN : 20864094     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences
HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.
Arjuna Subject : -
Articles 1,091 Documents
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The Inhibition of 15-Lipoxygenase by Blechnum orientale Leaves and its Glycoside-flavonoid Isolates: In Vitro and In Silico Studies Rissyelly Rissyelly; Syaikhul Aziz; Frangky Sangande; Agung Wibawa Mahatva Yodha; Krisyanti Budipramana; Elfahmi Elfahmi; Sukrasno Sukrasno
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.353-359

Abstract

Fern is one of the groups of primitive plants rich in secondary metabolites that are commonly used to treat various diseases, including antioxidant, anti-hyaluronidase, anti-inflammation, and respiratory disease but less investigated. Flavonoid is one of the secondary metabolites abundantly present in ferns. This study aims to isolate major compounds found in Blechnum orientale act as 15-lipoxygenase (15-LOX) inhibitors. Inhibition of lipoxygenase decrease the production of leukotriene that induces bronchoconstriction in asthma. Isoquercitrin (Quercetin-3-O-β-glucopyranoside) and trifolin (kaempferol-3-O-β-D-galactoside) have been successfully isolated from Blechnum orientale. Further in silico study was performed to explain the binding mode between flavonoid pyranoside or galactoside and flavonoid aglycone in the 15-LOX cavity and their amino acid residues interaction. Isoquercitrin binds with Ile663, Ile400, Leu408, Leu597, Ala404, and Arg403 in the 15-LOX cavity as a lipoxygenase inhibitor. Trifolin binds the same amino acids as isoquercetin with addition His366, Gln596, and Phe175. Both isoquercitrin and trifolin act as competitive inhibitors against lipoxygenase enzymes.
Cross-Species Amplification and Variability of Microsatellite DNA Markers in Domesticated Indonesian Mahseer; A Case Study with Tor soro, Tor douronensis and their Interspecific Hybrids Imron Imron; Agung Asrori; Khotibul Umam; Otong Zenal Arifin; Dessy Nurul Astuti
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.409-416

Abstract

Indonesian mahseer (Tor spp.) are freshwater species of high economic, cultural, and conservatory values. Owing to their high values and environmental degradation, the population of Tor fish gradually decreased, and domestication efforts have been made to conserve the population. This study was aimed to assess the cross-species amplification and microsatellite genetic diversity in Indonesian mahseer Tor soro (SS), Tor douronensis (DD), and their interspecific hybrids using primers developed for Tor putitora. Eleven primer sets were used to test for amplifiability and screen genetic diversity in 40 progenies derived from those three groups. Results showed that seven primer sets (64%) successfully amplified loci. Genetic screening using the three most consistently amplifying primers showed that the number of alleles in the three populations was low, ranging from 2 to 5 alleles. The observed heterozygosity (HO) was high ranging from 0.650 to 0.789, and the fixation index (FIS) was negative, indicating heterozygosity excess. In line with other parameters, the P-values of the HW parameter of several loci-population combinations were significantly departed from equilibrium (P <0.05). A few private alleles were observed in parental line DD and the hybrids. Overall, the cross-species primers developed from T. putitora were able to amplify loci in T. soro, T. douronensis and their hybrids and genetic diversity in the hybrid population was slightly higher than those in parental lines. Possible factors driving the phenomena and practical implications of these findings on the conservation measures are discussed.
Alkaline Phosphatase Activity of Plant Growth-Promoting Actinomycetes and Their Genetic Diversity Based on the phoD Gene Muhammad Faiz Amri; Edi Husen; Aris Tjahjoleksono; Aris Tri Wahyudi
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.360-369

Abstract

Actinomycete is one of the beneficial bacteria groups inhabiting rhizosphere soil. They can promote plant growth through various mechanisms. In the previous study we have isolated rhizosphere actinomycetes from maize rhizosphere with direct plant growth promotion characters. The aims of the present study were to analyze the ability of maize rhizosphere actinomycetes to solubilize phosphate, determine alkaline phosphatase activity, and study their genetic diversity based on phoD gene. Thirteen rhizosphere actinomycete isolates were able to solubilize phosphate at concertation range 55.84±2.27 mg/L to 144.48±5.71 mg/L. The activity of extracellular alkaline phosphatase was exhibited by all maize rhizosphere actinomycetes isolates in various level ranging from 0.08 mU/mL to 0.51 mU/mL. The phoD gene, one of the three homologous genes which encode alkaline phosphatases, was successfully detected in all isolates and identified as alkaline phosphatase D of Streptomyces spp. The partial phoD sequences of the isolates were located within metallophosphatase domain of alkaline phosphatase D. Alignment analysis showed that the deduced amino acid sequences of PhoD were mostly conserved in the isolates and Streptomyces spp. Essential residues involved in the active core arrangement of PhoD which binds metal ion cofactors were conserved. Constructed phylogenetic tree showed that the isolates were divided into two groups within PhoD cluster. PhoD of the isolates and Streptomyces spp. had closer relationship to purple acid phosphatase compared to other homologous PhoA and PhoX which form separate cluster. Generated three-dimensional structure model of partial PhoD had high similarity to alkaline phosphatase D of Bacillus subtilis (2YEQ) and showed overlapping structure based on super-positioning analysis.
DNA Barcoding of Neolissochilus sumatranus and Tor douronensis to Support In Situ Conservation of Indonesian Mahseers Sekar Larashati; Novi Mayasari; Yuli S Fitriana; Gema Wahyudewantoro; Mey R Widoretno; Tri Widiyanto
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.370-377

Abstract

The fishes of genera Neolissochilus and Tor are locally known as Ikan Batak or ihan in North Sumatra. They are sacred and are usually served in Batak tribe traditional ceremonies. However, their population in the wild is declining because of habitat degradation, unwise exploitation, water pollution, and the introduction of invasive species. Therefore, suitable management is needed to maintain Ikan Batak's sustainability in their natural habitat, which can be conducted through in situ conservation. The habitat of Ikan Batak in the Bonan Dolok river of the Samosir Regency can potentially be developed as a conservation site. Information concerning this species in Bonan Dolok River is needed as a basis for effective in situ conservation management. Morphologically identified fish captured in Bonan Dolok River were validated using DNA barcoding of cytochrome oxidase I (COI) mitochondrial DNA segment. This study confirmed that Ikan Batak captured from Bonan Dolok River belongs to two genera, Neolissochilus and Tor. Furthermore, the phylogenetic tree construction using Maximum Likelihood showed that the specimens identified as N. sumatranus were identical to N. cf soroides, a species found in the Malaysian peninsula, Thailand, and Cambodia.
Biocontrol Activity, Mode of Action, and Colonization of Aureobasidium pullulans Dmg 30 DEP on Controlling Early Blight Disease on Tomato Plant Wawan Setiawan; Suryo Wiyono; Toga Pangihotan Napitupulu; Atit Kanti; Idris Idris; Masrukhin Masrukhin; Efi Toding Tondok; I Nyoman Sumerta; I Made Sudiana
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.320-329

Abstract

We investigated the biocontrol activity of A. pullulans Dmg 30 DEP against Alternaria solani causal agent of early blight. Biocontrol activity was tested by the in vivo and ad planta. Biocontrol activity were tested by investigating the antibiosis capabilities with dual culture method, paper dish assay, two-compartment petri dish assay, and trapping and identification of volatile organic compounds (VOCs) with GC-MS. Lysis activity was examined by observing the clear zone formed by growing yeast on chitin agar and skim milk agar. The ability of hyperparasitism was assessed by the agar block method, and observed by light microscopy and scanning electron microscopy (SEM). The results showed that A. pullulans Dmg 30 DEP plays a role in the suppression of early blight disease at 106 cells/ml and 107 cells/ml yeast cell density. The mechanism involved in biocontrol activity is the production of VOCs, the production of chitinase and protease enzymes, the production of siderophore and hyperparasitism. The result shows that A. pullulans Dmg 30 DEP was colonizing the tomato leaves following the areole.
Potential of Conditioned Medium of hATMSCs in Aging Cells Model Wahyu Widowati; Rachmawati Noverina; Wireni Ayuningtyas; Dedy Kurniawan; Seila Arumwardana; Hanna Sari W Kusuma; Rizal Rizal; Dian Ratih Laksmitawati; Ratih Rinendyaputri; Rilianawati Rilianawati; Ahmad Faried
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.378-388

Abstract

Skin aging is caused by the exposure cumulative of ultraviolet radiation, it leads reactive oxygen species (ROS) production in the skin. The conditioned medium of human Adipose Tissue-derived Mesenchymal Stem Cells (hATMSCs) can scavenge free radicals and increase the survival rate of skin cells under oxidative stress. This study examined the protective effects of Conditioned Medium (CM) of hATMSCs in H2O2-induced human skin fibroblast cell line (BJ). The aging cells model using H2O2-induced BJ cells were added CM-hATMSCs in concentrations (0, 10, 30%) and incubated in various time, furthermore BJ cells induced by various H2O2 concentrations (0, 50, 100, 200 µM) incubated for 1 h. The anti-aging potential were measured including viability, ROS and collagen levels in BJ cells which treated CM-hATMSCs. The median inhibitory concentration (IC50) of H2O2 on BJ cells for 1 h incubation was 107.87 μM and 91.25 μM for 10 min incubation. CM-hATMSCs increased the viability on aging model cells. CM-hATMSCs concentration 30% increased the viability of H2O2 50, 100, 200 µM-induced BJ cells. CM-hATMSCs concentration 25% decreased ROS, increased collagen level in H2O2 50, 100, 200 µM-induced BJ cells. CM-hATMSCs increase the viability cells, collagen level and decrease ROS level in aging model cells.
In Silico Study of Haematococcus pluvialis Biomarker Compound as Supplement to Fish Bone Remodelling Daniar Kusumawati; Sri Widyarti; Maftuch Maftuch; Sri Rahayu
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.330-342

Abstract

This study aims to determine the docking predictions for RAR, RXR, and ROR in the bone remodeling pathway using Haematococcus, which is the highest carotenoid-producing microalgae. Furthermore, it determines the projection of using this carotenoid-producing alga in bone development. Carotenoids include provitamin A and non-provitamin A, which are predicted to replace vitamin A in bone control. It is also required in silico proof of carotenoids' function of bone remodeling control. Furthermore, molecular and visualization docking validation was conducted using PyRx and Discovery Studio Visualizer software respectively. According to binding affinity and RMSD value, each biomarker compound had particular binding sites on RARα, RARβ, RORβ, and RORγ. Astaxanthin was the only compound with binding sites on all four receptors. Through enzymatic action, provitamin A carotenoids can serve as a precursor to retinol, allowing them to act as a native RXR ligand. Therefore, the biomarker compound used in Haematococcus pluvialis can replace the role of vitamin A in the regulation of fish bones. The prediction of bone regulation in biomarker compounds through the RAR-RXR pathways inhibited osteoblast and osteclast. Otherwise, VDR-RXR pathways regulated osteoclast maturation and osteoblast mineralization.
Community of Soil Actinobacteria in PTPN VI Oil Palm Plantation Jambi (Sumatra, Indonesia) Based on Amplicon Sequencing of 16S rRNA Gene Mazidah Noer Inayah; Yulin Lestari; Anja Meryandini
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.389-398

Abstract

In Sumatra, Indonesia, increased oil palm production encourages land expansion for oil palm plantations. And soil Actinobacteria have a potential role in agriculture and plantations ecosystems. The use of fertilizer and herbicide affects soil microbial diversity, including Actinobacteria. This research analyzed and investigated the community composition and diversity of Actinobacteria in soils of oil palm plantations in Jambi Sumatra. Amplicon-based analysis of the 16S rRNA gene (V3-V4 hypervariable region) was used to amplify actinobacterial full-length 16S sequences. The V3-V4 actinobacterial specific 16S rRNA gene sequencing was done using Next-Generation Sequencing. This study confirmed that actinobacterial specific 16S rRNA gene primer could amplify the actinobacterial 16S rRNA gene. Frankiales dominated the community composition of soilborne Actinobacteria. The diversity and community composition of soilborne Actinobacteria were not significantly affected by the interaction between fertilization and weed treatments. Furthermore, the use of NPK fertilizer significantly affected the abundance of Kineosporiales, whose abundance increased with the increasing concentration of NPK fertilizer. The interaction between fertilization and weeding treatments in the oil palm plantations has no impact on soil Actinobacteria's community composition and diversity.
Taxonomy and Antimicrobial Activity of Gliding Bacterium from Indonesian Mangroves Senlie Octaviana; Tjandrawati Mozef; Joachim Wink
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.343-352

Abstract

The discovery of new antibiotic is needed, due to the increasing antimicrobial resistance in nature. Therefore, this study aims to isolate gliding bacteria and to ascertain their antimicrobial activity against pathogenic microbes. This research was conducted by isolating gliding bacteria from mangrove sediment in Muara Angke, North Jakarta, Indonesia. All of the strains were identified by 16S rRNA genes sequencing and those selected strain was characterized using polyphasic approach. The performance of crude extracts against to ten pathogenic microorganisms were detected using serial dilution test in 96-well plates. Mangrove gliding bacteria isolates designated 313MSO and 314MSO were showed high homology to Ohtaekwangia kribbensis with 96.20% and 99.12% similarity, respectively. The polyphasic approach led to the conclusion that the strain 313MSO was a new species of the genus Ohtaekwangia. Twenty-three crude extracts were obtained from cultivating the strain in twenty-three different media. The most of them inhibited the growth of Staphylococcus aureus Newman with the minimum concentration of 33.33-66.67 µg/ml. Four compounds (Marinoquinolines A-D) were obtained from HPLC-MS analysis. Furthermore, the strain 313MSO is presently being studied for in-depth identification of additional unknown metabolites detected in the crude extracts.
Population Analysis and Genetic Structure of Two Kazakh Cattle Breeds Using 150K SNP Indira Beishova; Kairat Dossybayev; Alzhan Shamshidin; Alena Belaya; Anuarbek Bissembayev; Kadyrzhan Khamzin; Alexandr Kovalchuk; Askar Nametov
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.301-309

Abstract

Kazakh white-headed and Auliekol breeds are mostly spread through Kazakhstan and are animals with dual purpose productivity directions including meat and milk. Kazakh native cattle breeds are still largely unexplored based on the genetic markers and are of great interest in the country. In this study, we for the first time applied high-density SNP genotyping for two indigenous cattle breeds to study genetic diversity, population structure and relationships with the different foreign breeds. Using of GGP Bovine 150K SNP array the principal component analysis (PCA), populations estimates of the genetic structure (ADMIXTURE), genetic diversity and distances (Fst), and phylogenetic tree showed that the Kazakh white-headed and Auliekol breeds have certain separate genetic structure that differ from European and Russian cattle breeds The two of these Kazakh cattle breeds have their genetic background according to observed results as distinct breeds. The obtained results will be aided in the development breeding system and conservation programs of the Kazakh white-headed and Auliekol breeds of beef cattle in the Kazakhstan.

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