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INDONESIA
HAYATI Journal of Biosciences
Published by Institut Pertanian Bogor
ISSN : 19783019     EISSN : 20864094     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences
HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.
Arjuna Subject : -
Articles 1,091 Documents
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In Vitro Pancreatic Lipase Inhibition by Marine Fungi Purpureocillium lilacinum Associated with Stylissa sp. Sponge as Anti-obesity Agent Wendi Nurul Fadillah; Nampiah Sukarno; Dyah Iswantini; Min Rahminiwati; Novriyandi Hanif; Mashuri Waite
HAYATI Journal of Biosciences Vol. 29 No. 1 (2022): January 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.1.76-86

Abstract

This study aimed to evaluate the potential of marine fungus Purpureocillium lilacinum isolated from an Indonesian marine sponge Stylissa sp. as an anti-obesity agent through pancreatic lipase inhibition assay. The fungus was identified as P. lilacinum through morphological and molecular characteristics. The fungal extract’s inhibition activity and kinetics were evaluated using spectrophotometry and Lineweaver-Burk plots. Ethyl acetate and butanol were used for extraction. Both extracts showed pancreatic lipase inhibition in a concentration-dependent manner. Both crude extracts were then fractionated once. All fractionated extracts showed inhibitory activity above 50%, with the highest activity found in fraction 5 of ethyl acetate at 93.41% inhibition. The best fractionated extract had an IC50value of 220.60 µg.mL-1. The most active fraction of P. lilacinum had a competitive-type inhibitor behavior as shown by the value of Vmax not significantly changing from 388.80 to 382.62 mM pNP.min-1, and the Michaelis-Menten constant (KM) increased from 2.02 to 5.47 mM in the presence of 500 µg.mL-1 fractionated extract. Metabolite identification with LC-MS/MS QTOF suggested that galangin, kaempferol, and quercetin were responsible for the observed lipase inhibition.
Antioxidant Activity of Endophytic Bacteria Derived from Hoya multiflora Blume Plant and Their Cellular Activities on Schizosaccharomyces pombe Nabila Tsoerayya Gustia Pudjas; Nisa Rachmania Mubarik; Rika Indri Astuti; Lisdar I Sudirman
HAYATI Journal of Biosciences Vol. 29 No. 2 (2022): March 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.2.214-221

Abstract

Endophytic bacteria isolated from plant tissues can produce the same secondary metabolites as their host plants. One of the metabolites that the bacteria can produce is antioxidants. This research aimed to analyze and measure the antioxidant activities of two endophytic bacteria, i.e. Bacillus siamensis HMB1 and Bacillus aryabhattai HMD4 cultures, derived from Hoya multiflora Blume plant, a tropical epiphytic plant species that grows in Indonesia, and to identify their cellular effects on Schizosaccharomyces pombe. The active compounds went through extraction process, and the antioxidant activities were measured, the extracts went under phytochemical analysis, and their phenol and flavonoid contents were measured. In vitro analysis was carried out using S. pombe. The results of this research indicated that both cultures had antioxidant activities, where HMB1 showed the highest IC50 value (51.18 mg/ml) among all. In vitro analysis indicated that HMD4 bacterial crude extract in 250 ppm concentration showed the highest resistance effect and significantly enhanced S. pombe growth. In addition, the results of the LC-MS analysis suggested that a total of 14 compounds potentially had antioxidant activity.
Leaf Litter Decomposition and Nutrient Release of Three Native Tree Species in a Drained Tropical Peatland in Riau, Indonesia Ahmad Junaedi; Nina Mindawati; Avry Pribadi; Suryo Hardiwinoto
HAYATI Journal of Biosciences Vol. 29 No. 2 (2022): March 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.2.182-191

Abstract

The decomposition and its nutrient release were the key ecological process that had a broad role in the forest ecosystem. This study aimed to investigate the leaf litter decomposition rate and its nutrient release of three native tree species of tropical peat swamp forest, namely Macaranga pruinosa, Macaranga gigantea, and Cratoxylum arborescens and one exotic species i.e Acacia crassicarpa. The decomposition and nutrient release were monitored in an experimental plot using litter bag technique. The initial litter quality of each litter and micro-environment properties were also observed. The result showed that the decomposition and its nutrient release were insignificantly different among native tree species and also between native species and Acacia crasssicarpa. The litter decomposition of all tree species was slow; with the range of k was 0.98-1.19 year-1. However, the P and K release from the decomposition of native species litter after four months of incubation were quickly, ranging 70-74% and 88-93%. We were suggested that the high of lignin content in the leaf litter (36-39%) was the main factor that made slow decomposition. These findings could be used as one of the tools in tree species selection for peat swamp forest rehabilitation.
Glycoengineering of Darbepoetin-α in CHO-DG44 Cells through Overexpression of α-2,3-sialyl-transferase and CMP-sialic Acid Transporter Adi Santoso; Endah Puji Septisetyani; Ratna Dwi Ramadani; Yana Rubiyana; Pekik Wiji Prasetyaningrum; Popi Hadi Wisnuwardhani; Arizah Kusumawati; Neny Nuraini
HAYATI Journal of Biosciences Vol. 29 No. 2 (2022): March 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.2.204-213

Abstract

Sialic acid plays a very important role in determining the circulation life span of glycoprotein in various organisms. Therefore, having a high content of sialic acid is needed by glycoprotein therapeutic agents to be able to function as desired. For example, Darbepoetin (DPO), the 5 N-linked erythropoietin showed higher bioavailability and efficacy compared to 3 N-linked erythropoietin. However, in the DPO production process, the molecular weight can vary and is highly dependent on the content of sialic acid and its production host. To improve the DPO sialic acid contents in our CHO-DG44 expressing DPO, we have engineered the cells through overexpression of α-2,3-sialyl-transferase (ST) and CMP-sialic acid transporter (CST). The DPO contained in the supernatant of the engineered cells was analyzed by Western blot and characterized by using PNGase-F or neuraminidase enzyme digestions. The results showed that, two clones, overexpressing ST or CST, were obtained. The clones showed higher molecular weight of DPO as compared to DPO expressed by the parental cells, yet retained the same protein backbone. The overexpression of these two genes does not affect cell growth. This suggests that may be these cells beneficial for therapeutic glycoproteins.
The Structural Annotations of The Mir-122 Non-Coding RNA from The Tilapia Fish (Oreochromis niloticus) Arli Aditya Parikesit; Imron Imron; Rizky Nurdiansyah; David Agustriawan
HAYATI Journal of Biosciences Vol. 29 No. 2 (2022): March 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.2.171-181

Abstract

Tilapia (Oreochromis niloticus) is an important fisheries commodity. Scientific efforts have been done to increase its quality. One of them is staging a premium diet such as a fat-enriched diet. The transcriptomics approach is able to provide the signatures of the diet outcomes by observing the micro(mi)RNA signature in transcriptional regulation. Hence, it was found that the availability of mir-122 is essential in the regulation of a high-fat diet in tilapia. However, this transcriptomics signature is lacking structural annotations and the complete interaction annotations with its silencing(si)RNA. RNAcentral website was navigated for the latest annotation of mir-122 from tilapia and other species as a comparison. MEGA X was employed to comprehend the miRNA evolutionary repertoire. The RNA secondary structure prediction tools from the Vienna RNA package and the RNA tertiary structure prediction tools from simRNA and modeRNA are secured with default parameters. The HNADOCK tools were leveraged to observe the interaction between mir-122 and its siRNA. The post-processing was conducted with the Chimera visualization tool. The secondary and tertiary structure of the mir-122 and its siRNA could be elucidated, docked, and visualized. In this end, further effort to develop a comprehensive molecular breeding tool could be secured with the structural annotation information.
Reef Fish in the Mudflats of Kaledupa Island in Wakatobi National Park, Indonesia Ernik Yuliana; Adi Winata; Hasan Eldin Adimu; Yuni Tri Hewindati; Wibowo A. Djatmiko
HAYATI Journal of Biosciences Vol. 29 No. 2 (2022): March 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.2.245-254

Abstract

Although frequently described as low-fertility or low-productivity habitat, coastal mudflats serve as important feeding grounds for fish. Many fish species from adjacent coral reefs, seagrass beds, or mangroves foraging periodically in mudflats. Because of this foraging behaviour, some local fishermen are known to utilize the mudflats to catch fish. However, the impact of this catching activities to the ecosystem has not been fully discovered. An examination of the fish community structure and levels of environmental stress had carried out in the mudflat ecosystem of the coast of Kaledupa Island in Wakatobi National Park (WNP), Indonesia. Two mudflat study sites were selected from the shore of Balasuna and Tampara villages located between mangroves and coral reefs. Data were sampled from the fish catch of local fishermen using fish fences (sero) installed in each mudflat area. Fish community structure was analyzed using diversity index and index of relative importance (IRI). ABC curves and species exploitation rate were used to assess the local environmental pressure. A total of 74 fish species were recorded from the mudflats of Kaledupa, which was found to be dominated by reef-associated fish species, comprising 63 species and accounting for 85% of the total catch. Additionally, although both sites had relatively high reef fish diversity, the obtained Clarke’s W-statistic values were approximately 0, indicating that the local fish communities presented moderate levels of disturbance. Three out of five fish species with the highest IRI values were found to be over-exploited, namely Siganus canaliculatus, Lethrinus ornatus, and Lethrinus variegatus.
Allometric Model, Aboveground Biomass and Carbon Sequestration of Natural Regeneration of Avicennia lanata (Ridley). at in-active Pond of Muna Regency, Southeast Sulawesi Fajar Hasidu; Arif Prasetya; Maharani Maharani; Muhammad Syaiful; Kangkuso Analuddin
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.399-408

Abstract

This study aimed to establish an allometric model for estimation of aboveground biomass, and carbon sequestration in A. lanata mangrove forest growing in Muna Regency, Southeast Sulawesi. Research methods were done by transect and 5 quadrats with size of 100 m2 each. A total of thirteen individual trees with different sizes were harvested. While DBH and D30 were measured. The samples were separated into stems, branches, and leaves and then weighted. The sample from each fresh organs were taken and brought to the Laboratory and then oven dried at 80°C for 7 days. The allometric equations were established by using independent variables (DBH and D30), and dependent variables (Ws, Wb, Wl). The partial and overall aboveground biomasses were calculated from allometric model, while carbon stock and CO2 sequestration were estimated. The results showed that the independent variable of DBH was more applicable for estimation of Ws, Wb, Wl, and total biomasses (Mg ha-1) of A. lanata forest, which were estimated as 28.28±3.48, 6.40±0.79, 5.00±0.66, and 40.08±4.97 respectively. The carbon stock in stems (13.24±1.63 Mg ha-1) was higher than in branches (3.01±0.37 Mg C ha-1) as well as in leaves (2.35±0.31 Mg C ha-1). The total of carbon stock were estimated at about 18.83±2.33 Mg C ha-1. Meanwhile, the total of CO2 absorption by A. lanata mangrove was 43.95±5.45 Mg CO2 ha-1. Therefore a regenerated A. lanata mangrove in this in-active pond area had potentiality on carbon stock and sequestrations, although these vegetation condition was still in the growth stage.
The Banana (Musa balbisiana Colla) Peels Flour Modulates HTR2B Receptor Expression in the Liver Diabetic Rats Andreanyta Meliala; Paramita Narwidina; Herlin Ajeng Nurrahma; Irwan Supriyanto
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.279-287

Abstract

Uncontrolled diabetes mellitus (DM) has been linked to depression and lipid metabolism impairment. Serotonin (5-HT) has been known to improve lipid metabolism and affect mood. The 5-Hydroxytryptophan (5-HTP) in banana peel flour (BPF) is a precursor to synthesize 5-HT in the body, which is linked to initiating liver regeneration via HTR2B receptors. The impact of 5-HTP in the diet on HTR2B receptor expression has yet to be thoroughly investigated. We aimed to elucidate the correlation between serum lipid profile and immobility time in Tail Suspension Test (TST) as depressive-like behavior and compare the expression of HTR2B receptor in healthy and diabetic rats. Male Wistar (Rattus norvegicus) rats were assigned to the control and treatment groups in a DM model with streptozotocin (60 mg/kg) injected intraperitoneally, then either fed standard diet or BPF 10% supplemented standard diet for 21 days. Immunohistochemical staining was used to examine the expression of 5-HT, HTR2B receptors. We determined that diabetic rats fed the standard diet supplemented 10% BPF group showed significantly lower concentrations of total cholesterol and triglycerides compared to diabetic rats fed a standard diet and it was positively correlated between total cholesterol and LDL with the duration of immobility time in TST. The average immunoreactivity score in diabetic rats fed 10% BPF was the highest among the other groups, indicating that the available BPF dose is sufficient for HTR2B activation, which will support the liver cell regeneration process, and should be investigated further.
The Potential Roles of Gut Microbiome in Modulating the Immune Response of Asian Redtail Catfish (Hemibagrus nemurus) Vaccinated with Aeromonas hydrophila Septyan Andriyanto; Yani Aryati; Tuti Sumiati; Angela Mariana Lusiastuti; Nurhidayat; Kurniawan Kurniawan; Rofingah Kuswatun; Nining Nuraeni
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.266-278

Abstract

The research aims are to observe the effect of vaccination in microbial profiles and gut microbiome composition. The treatments were as follows: the fishes were injected with PBS and challenged (A); the fishes were injected with freeze–dried vaccine dissolved in 100 ml 0.85% NaCl and challenged (B); the fishes were injected with freeze–dried vaccine dissolved in 50 ml 0.85% NaCl and challenged (C), and the fishes were injected with liquid vaccine and challenged (D). Microbiome composition measurements were carried out on the 21st–day post–vaccination and the 7th day after the challenge test. Fish intestine samples from three replications were tested by Next Generation Sequencing (NGS). Two significant phyla were identified from all treatments, namely Proteobacteria and Firmicutes. Cetobacterium, Candidatus Bacilloplasma, and Clostridium sensu stricto were the genera classified as good bacteria in vaccinated fish. It can be concluded that vaccination can increase the diversity of the gut microbiome, especially bacteria beneficial to the fish host. Chitosan as a coating antigen in freeze–dried vaccine increases gut microbiome's number and diversity better than a liquid vaccine.
Cloning, Expression, and In Silico Analysis of Class IV Poly-(R)-3-hydroxybutyrate Genes from New Strain of Bacillus thuringiensis TH-01 Zuhdina Sabiqoh; Rukman Hertadi; Enny Ratnaningsih
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.310-319

Abstract

Poly-(R)-3-hydroxybutyrate (PHB) is a bioplastic derivative of polyhydroxyalkanoate (PHA) which can be synthesized by bacteria under certain growth conditions. Previous study has reported a new strain of Bacillus thuringiensis TH-01 isolated from thermite, which found to accumulate PHB. This research aimed to clone PHB biosynthesis genes from B. thuringiensis TH-01 and study its expression as well as predict the tertiary structure of the enzymes. The clone of phaA gene, which encodes PhaA, was obtained as 1182 bp. On the other hand, 2546 bp clone of phaRBC gene cluster was obtained to consist of 744 bp phaB, 1086 bp phaC, and 483 bp phaR, encoding respective PhaB, PhaC, and PhaR proteins. In silico analysis indicated that PhaA, PhaB, PhaC, and PhaR, revealed to have 393, 247, 361, and 160 amino acid, respectively. The predicted model of PhaA, PhaB, and PhaC showed dominant structure of α/β folding motif, while PhaR was dominated by a helix-loop-helix motif. The catalytic residues of PhaA were Cys88, His349, and Cys379, whereas the catalytic residues of PhaB were Ser142, Tyr155, and Lys159. These catalytic residues were identical to those residues obtained in other PHB biosynthetic enzymes reported elsewhere, confirming that our clones were of PHB biosynthetic genes.

Page 58 of 110 | Total Record : 1091

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