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INDONESIA
HAYATI Journal of Biosciences
Published by Institut Pertanian Bogor
ISSN : 19783019     EISSN : 20864094     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences
HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.
Arjuna Subject : -
Articles 1,091 Documents
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The Effect of Cang Salak Tea Diet on Apolipoprotein C3 (ApoC3) Gene Expression on Hyperlipidemic Rats Model Burhannuddin; I Wayan Karta; I Gusti Ngurah Dwija Putra; Felicia Kartawidjajaputra
HAYATI Journal of Biosciences Vol. 29 No. 6 (2022): November 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.6.814-823

Abstract

Cardiovascular disease (CVD) is the leading cause of global mortality and disability. Hyperlipidemia is a major risk factor for CVD that can be controlled through medical therapy, appropriate nutrition, and lifestyle. This study aimed to identify the cang salak tea diet’s effect on the ApoC3 gene expression in a hyperlipidemia rat. 18 male Wistar rats were divided equally into three groups. A high-fat-diet-induced two groups of rats, and one group was the control. Once hyperlipidemia had been achieved, one of the two groups was treated with the cang salak tea, and one group was given a standard diet for four weeks. Authenticated rat and liver tissue were collected as a source of RNA isolation. Isolated RNA was used as a reaction template for the relative quantitation qPCR using β-actin as the housekeeping gene. The ApoC3 gene was specifically amplified with a Tm value of 82.73°C, Cq 17-19, and produced a sigmoid curve. The relative expression level of the ApoC3 gene in hyperlipidemia rats fed with the cang salak tea diet was 0.46 times significantly lower than the control (1.17) and P2 (1.32) groups. These results indicate that the cang salak tea has antihyperlipidemic properties to reduce CVD risk.
Determination of Complete Sequence Mutation of Myostatin Gene in Fast- and Slow-Growing Chicken Isyana Khaerunnisa; Ahmad Furqon; Saiful Anwar; Jakaria Jakaria; Cahyo Budiman; Irma Isnafia Arief; Cece Sumantri; Yong Soo Kim
HAYATI Journal of Biosciences Vol. 30 No. 1 (2023): January 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.1.148-158

Abstract

Myostatin plays a role in inhibiting skeletal muscle growth in vertebrates. This study aimed to investigate the full sequence of the myostatin gene in fast-growing and slow-growing chickens. Fast- and slow-growing chicken models were produced from F2 Kampung x broiler. The full sequence of the myostatin gene was identified using 24 pairs of primers covering about 8,000 bp. mRNA expression analysis of muscle tissue was performed to examine whether the expression levels of myostatin are affected by chicken lines, sex, or muscle type. The results showed 170 mutations in fast- and slow-growing chickens. One hundred and sixty-one of them are novel mutations. A total of five and twenty-two alleles were specific alleles found only in the fast-growing and slow-growing groups of chickens, respectively. There were no differences in amino acids and gene expression levels of myostatin between the fast- and slow-growing chickens. In summary, the results of this study showed that specific alleles for the fast-growing or slow-growing chicken groups were found, suggesting that these specific alleles potentially be used as genetic markers for muscle growth in chickens.
Genetic Diversity of Eight Native Indonesian Chicken Breeds on Microsatellite Markers Tike Sartika; Ferdy Saputra; Hideaki Takahashi
HAYATI Journal of Biosciences Vol. 30 No. 1 (2023): January 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.1.122-130

Abstract

Indonesia has diversity in native chickens based on phenotypes. This diversity is utilized for economic purposes such as meat, eggs, fancy, crowing, and fighting. This study aimed to determine the genetic structure of eight native Indonesian chicken breeds with microsatellite markers, the genetic distance, and inbreeding coefficient of each breed of chicken used for crossbreeding programs to obtain a positive heterosis effect for selection programs. The samples used were Arab, Merawang, Pelung, Sentul, Cemani, KUB, Black Kedu and White Kedu. Broiler chickens (Cobb) were used as the outgroup in this study. A total of 192 DNA samples from eight breeds were used in this study. A total of 24 microsatellite markers were used in this study to observe the genetic diversity of 8 native breeds. The POPGENE, Cervus, and FSTAT were used to generate the observed number of alleles, the effective number of alleles, observed heterozygosity value, expected heterozygosity value, the heterozygote deficit within the breed (FIS), gene flow (Nm), Hardy-Weinberg equilibrium, Polymorphism Information Content (PIC), and UPGMA tree. The principal component analysis (PCA) was performed using adegenet package of R software. Bayesian clustering assignments were analyzed using the STRUCTURE program. This study revealed a very close genetic relationship between seven native chickens and broilers. We also found Arab chickens separated from other Indonesian native chickens and no inbreeding in eight native Indonesian chicken breeds. In conclusion, we found two clusters among eight native Indonesian chicken breeds. Twenty microsatellite markers have a high PIC value in this study.
Some Hatchery Parameters of Three Species of Groupers: Tiger Grouper (Epinephelus fuscoguttatus), Humpback Grouper (Cromileptes altivelis), and Leopard Coral Grouper (Plectropomus leopardus) Suko Ismi; Darmawan Setia Budi
HAYATI Journal of Biosciences Vol. 29 No. 6 (2022): November 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.6.762-770

Abstract

Three species of groupers, including tiger grouper (Epinephelus fuscoguttatus), humpback grouper (Cromileptes altivelis), and leopard coral grouper (Plectropomus leopardus), have been successfully cultivated. However, the constraints on larviculture that have been described still arise in the hatchery. This study compares several hatchery parameters in these three grouper species representing three different genera (Epinephelus, Cromileptes, and Plectropomus). The egg incubation and larval rearing were carried out in a concrete tank (3 x 3 x 1.2 m3) filled with 6-7 m3 seawater. Eggs were stocked with a density of 100,000 eggs/tank. The grouper larvae were reared until they became juvenile. The eggs were incubated until they were hatched in the same container and media as larval rearing. Larvae were reared until 60 dah (days after hatching), and data were taken from the rearing for three cycle's larval culture. Management of grouper larval rearing follows the Good Fish Hatchery Method (Cara Pembenihan Ikan yang Baik/CPIB) based on Indonesian National Standard (Standar Nasional Indonesia/SNI). The experimental parameters were egg diameter, hatching rate, larvae mouth opening width, survival activity index (SAI), larvae development, and total body length growth. The several hatchery parameters in three grouper fish species are significantly different. The tiger grouper has the best larval performance.
Effect of Media Variation on the Induction and Phytochemical Profile of Callus in Two Varieties of Cat's Whiskers (Orthosiphon aristatus Blume Miq) Fahrauk Faramayuda; Akhirul Kahfi Syam; Totik Sri Mariani; Elfahmi; Sukrasno
HAYATI Journal of Biosciences Vol. 30 No. 1 (2023): January 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.1.159-170

Abstract

The levels of rosmarinic acid and sinensetin in purple and white-purple varieties of Orthosiphon aristatus, cat's whiskers, can be increased using modified in vitro culture. This work focused on callus induction of the purple and white-purple variety of cat's whiskers grown on Gamborg (B5) and CHU (N6) with the addition of growth regulators 2,4-dichlorophenoxyacetis acid. Our observation suggested that the callus could grow within three weeks and produce rosmarinic acid and sinensetin. The level of sinensetin from various extraction methods is relatively low; in contrast, the rosmarinic acid from purple callus was detected at about 5% w/w, while the white-purple variety was around 2% w/w. The results of this study also provided new information on the basic media other than MS that can grow cat's whiskers callus while producing active compounds.
Antibacterial and Antioxidant Activities of Fungal Endophytes Isolated from Medicinal Plants in Simeulue Island, Aceh Ahmad Fathoni; Muhammad Ilyas; Praptiwi; Dewi Wulansari; Andria Agusta
HAYATI Journal of Biosciences Vol. 29 No. 6 (2022): November 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.6.720-732

Abstract

Natural products from endophytic fungi have a wide range of medicinal applications, including antibacterial and antioxidant agents. The research aimed to evaluate the antibacterial and antioxidant activities of compounds derived from endophytic fungi isolated from medicinal plants collected from Simeulue Island by TLC dot-blot and microdilution assays. Eighty-one fungal extracts were screened for antibacterial and antioxidant activities. Antibacterial activity was evaluated against Gram-positive and negative bacteria, i.e., Staphylococcus aureus InaCC B-4 and Escherichia coli InaCC B-5, respectively. Antioxidant activity was carried out by using free radical scavenging of 1,1-diphenyl-2-picrylhydrazyl (DPPH). The results of the TLC bioassay revealed that the fungal extracts have activities as antibacterial and antioxidant are 65 (against S. aureus), 57 (against E. coli), and 48 extracts (DPPH free radical scavenger). Two extracts, Phomopsis sp. 48BtSi-2.1 and Xylaria sp. 55DnSi-1.1, showed strong antibacterial activity against S. aureus with MIC value of 32 μg/ml. Furthermore, nine extracts (Schizophyllum sp. 20DnSi-1; Hyphomycetes 36BhSi-1.1; Phomopsis sp. 36DnSi-2.1; Schizophyllum sp. 39DnSi-1.1; Xylaria sp. 39RpSi-2.1; Phomopsis sp. 41BtSi-1.1; Phomopsis sp. 48BtSi-2.1; Lasiodiplodia sp. 48BtSi-3.1; Schizophyllum sp. 50DnSi-3) were strong activity against E. coli with MIC value ranges from 32 to 64 μg/ml. One extract, Xylaria sp. 04BtSi-2.2 has strong antioxidant activity as DPPH free radical scavenger with an IC50 value of 21.66 μg/ml (AAI value of >1).
Biological Aspects and Feeding Ecology of Sembilang Plotosus canius in Langsa Estuary Agus Putra Abdul Samad; Nur Fazillah Nur Fazillah; Rindhira Humairani; Ilhamdi; Nan Fan Hua
HAYATI Journal of Biosciences Vol. 29 No. 6 (2022): November 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.6.782-788

Abstract

The knowledge of the biological aspects and feeding activity of fish is required to manage the lack of data on fisheries. In this study, a total of 200 Plotosus canius were caught and examined to obtain information on their biological aspects, length-weight relationship, and feeding activity. The fish were captured using a gill net and traditional fishing trap called Bubu. Subsequently, all samples were examined for total body weight, total length, condition factor, gonad maturity, and feeding activity. The results showed that the specimens captured were 156 males and 44 females weighing between 95-280 gr and 117-214 gr with the length of 26-39 cm and 28-36.3 cm, respectively. The length-weight relationship showed a negative allometric growth pattern. The mean condition factors of males and females were 1.1048 and 1.0102, respectively. At the same time, the maturity level of gonads was at I and II. Moreover, it was discovered that most organisms in the digestive tract were shrimp, worms, small fish, and crabs, while the highest index of preponderance was in shrimp with 42%. In conclusion, this study showed the new information about length-weight, biological condition, and feeding habits of Plotosus canius, which can be inventoried to strengthen data on sustainable fisheries management.
Anti-Inflammatory Effect of Isolate Compounds from Ethyl Acetate Fraction of Piper crocatum Ruiz and Pav Leaves on Lipopolysaccharide-induced RAW 264.7 Cells Nilda Lely; . Dachriyanus; Yufi Aldi; . Almahdy; Fatma Sri Wahyuni
HAYATI Journal of Biosciences Vol. 30 No. 1 (2023): January 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.1.28-34

Abstract

Red betel (Piper crocatum Ruiz and Pav) is a traditional Indonesian plant. The ethyl acetate fraction of red betel leaf has the potential as an anti-inflammatory. This research aimed to determine the anti-inflammatory effect of isolate compound from ethyl acetate fraction of Piper crocatum leaves on the production of cytokine pro-inflammatory TNF-α and ICAM-1 in lipopolysaccharide-induced RAW 264.7 cells. Cell viability was determined by the MTT method in the concentration range of 1.25; 2.5; 5; 10; 20, and 40 µg/ml. The TNF-α and ICAM-1 level was determined by the TNF-α and ICAM-1 assay kit as measured by an ELISA plate reader. Cell viability test of isolate compound from ethyl acetate fraction, the concentrations that gave viability percentages above 80% were 10; 5; 2.5 and 1.25 ug/ml. Isolate compound at concentrations of 10, 5, 2.5 and 1.25 ug/ml significantly inhibited TNF-α and ICAM-1 production with p<0.05 (p = 0.000).
Cold Stress Response Genes of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Lantiplantibacillus plantarum subsp. plantarum Mut-7 Support the Ability to Survive in Low-Temperature Conditions Eunike Marganingrum Andriani Samodra; Dian Suroto; Tyas Utami; Rachma Wikandari; Endang Sutriswati Rahayu
HAYATI Journal of Biosciences Vol. 30 No. 1 (2023): January 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.1.65-70

Abstract

Probiotics are widely consumed in various food matrices to provide health benefits to the host. The viability of probiotic cells is influenced by several factors, including exposure to high temperatures during the production process and low temperatures during storage. In this study, we report the response to cold stress of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Mut-7 after 24 h of storage at 4°C and -20℃. The cell number of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Mut-7 in low-temperature condition had no significant differences than their initial number: 11.88 log CFU/ml and 11.62 log CFU/ml at 4°C; 11.51 log CFU/ml and 11.47 log CFU/ml at -20°C for Mut-3 and Mut-7 respectively. The results indicated the survival capacity of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Mut-7 at low temperatures. The genes encoding cold shock proteins for the response to cold stress were evaluated by genome sequencing. The CspA/CspC genes of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Mut-7 possibly play a role in maintaining cell resistance at low temperatures, since the genes products predicted to have conserved motifs in the RNA binding protein (RNP) -1 and RNP-2 responsible for cold response stress which are similar to those in other bacteria.
Carbon-nanotube for Transient Expression in Rice Calli Paphawarin Pinyokham; Kamolwan Khianchaikhan; Pongsakorn Sunvittayakul; Supachai Vuttipongchaikij; Piyama Tasanasuwan; Chatchawan Jantasuriyarat
HAYATI Journal of Biosciences Vol. 29 No. 6 (2022): November 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.6.845-850

Abstract

Transient gene expression is an important technique in gene functional analysis, protein production and in plants. However, traditional transient expression methods using Agrobacterium are time-consuming with low efficiency. In this study, we demonstrated the use of a single-walled carbon nanotube (SWCNT) to deliver 35S:mCherry:pCXSN plasmid into rice calli. This transient expression protocol used a plastic medical syringe to create the physical pressure to help the delivery of plasmid DNA into plant cells. This protocol is relatively easy to perform and low cost. The transient expression was observed under fluorescence microscopy, and the mCherry fluorescence signal was quantified. The plasmid DNA was delivered into the rice cell using a 3:1 ratio (plasmid: carbon nanotube). The result showed that the mCherry signal of carbon nanotube + plasmid DNA treatment was the highest signal at 3 days post-transformation and decreased to a lower signal at 6 days post-transformation. Moreover, the quantitative analysis of mCherry mean intensity revealed that the signal intensity of carbon nanotube + plasmid DNA treatment was the highest level, and significantly higher than the control treatments at 3 days post-transformation. Plasmid DNA can be transported easily into plant calli using this carbon nanotube transient expression protocol.

Page 64 of 110 | Total Record : 1091

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