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Anakes: Jurnal Ilmiah Analis Kesehatan
Published by Universitas Mohammad Husni Thamrin
ISSN : 20885687     EISSN : 27456099     DOI : 10.37012/anakes
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology
Jurnal Ilmiah Analis Kesehatan merupakan jurnal yang berisi tentang artikel ilmiah dalam bidang ilmu analis kesehatan yang dilakukan oleh dosen maupun mahasiswa serta para peneliti.
Arjuna Subject : Profesi Kesehatan - Profesi Kesehatan (Lain-Lain)
Articles 28 Documents
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Search results for , issue "Vol. 11 No. 1 (2025): ANAKES: Jurnal Ilmiah Analis Kesehatan" : 28 Documents clear
Deteksi Dini Hipertensi Menggunakan Cold Pressure Test Pada Mahasiswa Fakultas Kedokteran Universitas Gunadarma Tahun 2025 Ningsih, Sri Rahayu; Sadewa, Rafi Abraar; Suhartini, Srimukti; Sudomo, Danurwendo
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 11 No. 1 (2025): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v11i1.2653

Abstract

Hypertension is one of the major non-communicable diseases (NCDs) that causes serious damage to blood vessels and vital organs such as the heart, kidneys, and brain. The Indonesian Health Survey reported that the prevalence of hypertension among adolescents aged 15–24 years reached 9.3%. According to the Basic Priority Rating (BPR) from the Depok City Health Office, hypertension ranks as the top NCD priority with a score of 38.69%. Early detection is essential to prevent delays in diagnosis and treatment. One method that can be used for early detection of hypertension in adolescents is the Cold Pressure Test (CPT). This study aimed to determine changes in systolic and diastolic blood pressure using the CPT method. This was an observational study with a cross-sectional design involving 43 medical students as participants. Blood pressure was measured at three stages: before hand immersion in ice water, after 30 seconds, and after 60 seconds. Data were analyzed using the Friedman test to assess differences across the three measurements. The results showed that systolic blood pressure increased from 117.35 mmHg to 132.12 mmHg at 60 seconds, while diastolic blood pressure increased from 79.07 mmHg to 88.74 mmHg. Statistical analysis revealed a p-value of 0.001 (< 0.005) for both systolic and diastolic pressure. The study concluded that there was a significant increase in systolic and diastolic blood pressure after the CPT. This indicates that adolescent blood pressure is reactive to cold stimuli, and CPT may serve as an effective method for early detection of hypertension. Keywords: Cold Pressure Test, Hypertension, Blood Pressure, Pukse
Gambaran Masa Pemulihan Sistem Kardiovaskular Berdasarkan Indeks Masa Tubuh Pada Mahasiswa Fakultas Kedokteran Universitas Gunadarma Zulyani, Febriyanti; Millenia, Fadya; Suhartini, Srimukti; Ningsih, Sri Rahayu
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 11 No. 1 (2025): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v11i1.2658

Abstract

Physical activity encompasses all body movements carried out for work, play, or travel, while physical inactivity is characterized byminimal movement and low energy expenditure. Body Mass Index (BMI) and recovery heart rate are important indicators in assessingfitness levels and health risks. This study aims to determine whether there are differences in cardiovascular recovery time after physicalactivity based on BMI categories among medical students at Gunadarma University. The study employs a cross-sectional design with purposive sampling among FKUG students who meet the inclusion criteria. Data were analyzed using paired T-tests and One WayANOVA according to data distribution. The procedure consists of two stages: measuring blood pressure, heart rate, O₂ saturation, and BMI before and after a six-minute walk test, followed by measuring heart rate recovery time after approximately 20 minutes of rest. Most respondents were female (92.5%) from the 2019 cohort (77.4%), with an average age of 20.79 years. A significant increase was observedin systolic blood pressure and heart rate after training (p=0.001), but not in diastolic pressure (p=0.622). No significant difference wasfound in recovery time based on BMI category (p=0.516). Physical training significantly affected certain physiological parameters such as systolic pressure and heart rate, but BMI status did not significantly contribute to post-activity recovery speed.  Keywords:     Physical Activity, Body Mass Index, Medical Students, Cardiovascular Recovery,  Pulse Rate
Variasi Konsentrasi Neutral Buffer Formalin Pada Proses Fiksasi Jaringan Histologi Ginjal Mencit Kayni, Ika Mareta Nur; Nailufar, Yuyun; Rahmawati, Yeni
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 11 No. 1 (2025): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v11i1.2685

Abstract

Neutral Buffer Formalin 10% is commonly used to fix tissue whose structure will be observed. The observation is carried out to diagnose the disease. Poor fixation results will affect further tissue processing. The right concentration and fixation time will also produce quality tissue preparations and the right diagnosis. This study determined the quality of the hematoxylin-eosin staining results in the mouse kidney fixation process with variations in NBF concentrations of 5%, 10%, 15%, and 20%. This research method is observational by fixing the mouse kidney organ in NBF with concentrations of 5%, 10%, 15%, and 20% for 24 hours at room temperature. The results of the study are in the form of scores, which are then processed using the Kruskal-Wallis Test. Based on the total scoring results, NBF 10% is the best concentration. The result of the Kruskal-Wallis test is p ≤ 0.05, which means there is a significant difference, and the Posthoc test result is that the NBF concentration of 15% is a concentration that has no significant difference with the control. While NBF 5% and 20% have significant differences from the NBF 10% control. Thus, NBF 10% is the best concentration, and NBF 15% has better assessment results than NBF 5% and 20%. Keywords: Fixation, Neutral Buffer Formalin, Hematoxylin Eosin
Analisis Kadar Karboksihemoglobin (COHb) dalam Darah Mahasiswa Perokok dan Bukan Perokok di Program Studi Teknologi Laboratorium Medis Universitas Mohammad Husni Thamrin Ramadres, Gerab; Masdianto, Masdianto; Kristianingsih, Yuli; Sugiantari, Nining
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 11 No. 1 (2025): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v11i1.2475

Abstract

Smoking is a common behavior among adolescents. Consequently, it can affect the heart, central nervous system, and all oxygen-sensitive organs. This study aimed to determine differences in COHb levels in the blood of smokers and nonsmokers in the Medical Laboratory Technology Study Program at Universitas Mohammad Husni Thamrin. The Conway diffusion cell method, using sulfuric acid, was used to release carbon monoxide from hemoglobin molecules. UV-Vis spectrophotometry was used to identify single substances. COHb levels in 15 smokers were 0.53% and 0.26% in 15 nonsmokers. The study concluded that there were differences in COHb levels between smokers and nonsmokers. Both COHb levels were below the normal value of 3.5%, as determined by the Indonesian Minister of Health Regulation No. 70 of 2016. Keywords : Blood, COHb level, smoker  
Sensitivitas Antara Antibiotik Meropenem dan Seftazidin Terhadap Pseudomonas aeruginosa Strain ATCC 15442 Pada Pasien Pneumonia di RSUP dr. Cipto Mangunkusumo Jakarta Pusat Diah Lestari; Husyain Djajaningrat; Febri Wulan Dari; Dean Handimulya
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 11 No. 1 (2025): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v11i1.2500

Abstract

Pneumonia is an acute lower respiratory tract infection in the lung parenchyma that has a high level of treatment resistance and is a nosocomial bacterial transmission. One of the nosocomial bacteria in hospitals is Pseudomonas aeruginosa, which is a Multi Drug Resistant (MDR) bacteria, a pathogen that causes infections that are resistant to at least three antimicrobial agents. The antimicrobials included are meropenem and ceftazidime, these two antibiotics are the best choice of last-line drugs in the treatment of MDR bacteria. Effective prevention and treatment of bacterial colonization and infection in pneumonia patients needs to be done to reduce the negative impact of antibiotic use. The aim of this study was to determine the sensitivity between the antibiotics meropenem and ceftazidime against Pseudomonas aeruginosa bacteria in pneumonia patients at RSUPN dr. Cipto Mangunkusumo, Central Jakarta. The research method was analytical observational using a cross sectional design with medical record data sources on 105 pneumonia patients who underwent sputum culture examination. Data analysis using the Mann-Whitney Test at 95% CI (α = 0.05). The research results showed that the majority of patients were male, 69 people with an elderly age category of 51 people (48.6%). The results of the antibiotic sensitivity of meropenem were 76.25% and ceftazidime were 69.5%, with a p-value of 0.000. The average sensitivity of the antibiotic meropenem is 20.59, more sensitive than the antibiotic ceftazidime, an average of 20.50 with a difference in sensitivity of 9%. The conclusion is that there is a difference in the sensitivity of the antibiotics meropenem and ceftazidime against Pseudomonas aeruginosa bacteria in pneumonia patients at RSUPN Dr. Cipto Mangunkusumo, Central Jakarta, the antibiotic meropenem is more sensitive than the antibiotic ceftazidime.  Keywords: Meropenem, Pneumonia, Pseudomonas aeruginosa,  Seftazidim.
Perbedaan Kadar Ureum Kreatinin Pada Penderita Gagal Ginjal Kronis Sebelum dan Sesudah Hemodialisa Di Rumah Sakit Wijaya Kusuma Purwokerto Herowati, Nunuk; Widyayanti, Oksita Asri; Mohtar, Cici Farhana Ambarwaty
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 11 No. 1 (2025): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v11i1.2506

Abstract

Chronic kidney failure (CKF) is a progressive and fatal disease in which the kidneys are no longer able to maintain metabolism, fluid and electrolyte balance Chronic Kidney Failure if the GFR value is <60ml/minute, the prevalence of Chronic Kidney Failure (CKF) worldwide has increased by 2.3%. The incidence of Chronic Kidney Failure (CKF) in Indonesia is 0.38% of the total population of Indonesia. Hemodialysis is a blood filtration treatment method used to remove fluids and waste that accumulate in the blood and cause poisoning when the kidneys experience serious disorders or gradually cannot function. The purpose of this study was to determine the difference in creatinine urea levels in patients with chronic kidney failure (CKF) before and after hemodialysis at the Level III Hospital 04.06.01 Wijaya Kusuma Purwokerto. This study used a quantitative descriptive method, with a sample size of 64 people. Data analysis was carried out using SPSS with a paired sample test. The results of the urea level study obtained an average urea level in patients of 155.34. The minimum value obtained was 72 and the maximum value was 273. The urea level after hemodialysis obtained an average of 95.92. The minimum value was 43 and the maximum value was 273. The creatinine level before hemodialysis obtained results where the average was 36.82, the minimum value was 2.23 and the maximum value was 14.10. The average creatinine level after hemodialysis obtained a minimum value of 1.33 and a maximum of 14.10 with an average of 7.29. The conclusion is that there is a significant difference in urea and creatinine levels before and after hemodialysis with the results of statistical tests obtained a value of p = 0.000.  Keywords: Acute Renal Failure, Chronic Renal Failure, Hemodialysis, Creatinine, Urine
Potensi Propolis dan Kombinasi Dengan Klorokuin Sebagai Antimalaria Pada Mencit (Mus Musculus) yang Terinfeksi Plasmodium berghei Astuty, Hendri; Farhan, Muhamad; Winita, Rawina
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 11 No. 1 (2025): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v11i1.2610

Abstract

Malaria is still a public health problem both in Southeast Asia and other regions of the world. The emergence of resistance to several malaria drugs, including chloroquine. With the increase in malaria resistance, including chloroquine. With the increase in malaria drug resistance, many studies have been conducted to find new antimalarial compounds. One of them is propolis which contains luteolin 7-O glucoside which can inhibit the biosynthesis of parasitic type 2 fatty acids and chalcone can inhibit the hemolysis process. This study aims to examine the therapeutic potential of propolis single and combination with chloroquine in male Mus musculus infected with Plasmodium berghei. The single propolis tested were 30 mg/kgBW, 60 mg/kgBW and a combination of propolis.  This study is an experimental laboratory study with a post-test control group-only design. Comparison of parasitemia density with the 4-day suppression test method in all treatment groups with the Kruskal-Wallis test found a p-value <0.001. The results study showed that single propolis at a dose of 30 mg/kg BW was better than a dose of 60 mg/kg BW. Combination therapy between propolis and chloroquine at doses of  30 mg/kg BB and 60 mg/kg BB had significant results. The conclusion of this study shows that combination therapy of propolis at a dose of 30 mg/kg WB or a dose of 60 mg/kg BB with chloroquine has better antimalarial potential, so propolis is synergistic with chloroquine as an antimalarial.  Keywords: Propolis , Chloroquine, Plasmodium berghei, Parasitemia
Uji Daya Hambat Ekstrak Bawang Dayak (Eleutherine palmifolia (L.) Merr.) Terhadap Pertumbuhan Bakteri Salmonella typhi Foekh, Neiny; Utami, Ayu Putri; Aisy, Dzia Ulhaq Rohadatul
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 11 No. 1 (2025): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v11i1.2612

Abstract

Dayak onion (Eleutherine palmifolia (L.) Merr) contains many active compounds that have the potential as antibacterial, including against Salmonella typhi bacteria. This study aimed to determine the effectiveness of dayak onion powder (Eleutherine palmifolia (L.) Merr) and to determine the minimum inhibitory concentration of dayak onion extract (Eleutherine palmifolia (L.) Merr) in inhibiting the growth of Salmonella typhi bacteria. Antibacterial testing was carried out using disc diffusion, by looking at the clear area (inhibition zone) around the disc.. The design of this study was descriptive research and the results of the analysis were carried out using the Kruskal Wallis statistical test. The results showed that the extract of dayak onion (Eleutherine palmifolia (L.) Merr) at concentrations of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100% had the ability to inhibit growth of Salmonella typhi bacteria. The average area of ​​inhibition of dayak onion extract (Eleutherine palmifolia (L.) Merr) was 7 mm, respectively; 7.3 mm; 7.6mm; 8mm; 8.3 mm; 8.6 mm; 9mm; 9.3 mm; 9.6 mm; 10.3mm. The greatest concentration of inhibition was 100% concentration with a diameter of 10.3 mm. In the blank disc negative control there was no inhibition of the growth of Salmonella typhi bacteria and in the positive control amoxicillin 500 mg there was an inhibition of 24 mm. Dayak onion extract (Eleutherine palmifolia (L.) Merr) can inhibit the growth of Salmonella typhi bacteria in the resistant category (weak) with the minimum inhibitory concentration of dayak onion extract (Eleutherine palmifolia (L.) Merr) against the growth of Salmonella typhi bacteria is 10% with an inhibition zone diameter of 7 mm.  Keywords: Dayak onion (Eleutherine palmifolia (L.) Merr), Salmonella typhi, Inhibitory test
Perbandingan Kadar Hemoglobin Post-Transfusi Whole Blood dan Packed Red Cells Pada Pasien Post-Operasi Sectio Caesarea Novita Nur Awaliyah; Dewi Astuti; Mike Rezeki Sugiarti; Eva Ayu Maharani; Puji Lestari
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 11 No. 1 (2025): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v11i1.2613

Abstract

Sectio Caesarea (SC) has a risk of bleeding that leads to a decrease in the mother's hemoglobin levels. Transfusion using Whole Blood (WB) and Packed Red Cells (PRC) blood components is a prosedure to increase hemoglobin levels.This study uses a cross-sectionally comparative analytical study designs on 96 secondary data from post-operative Sectio Caesarea (SC) patients who performed pre- and post-transfusion hemoglobin examination of Whole Blood (WB) and Packed Red Cells (PRC) during the period January-December 2023. The data were analyzed in the Kolmogorov-Smirnov univariat test, then in the Non-Parametric test (Mann-Whitney test) with a degree of confidence of 90%. The results obtained haemoglobin post- transfusion of Whole Blood (WB) had a range of values of 0.0 – 2.7 g/dL with an average value of 0.5 g/ dL, whereas the haemoglobin post- transfusion Packed Red Cells (PRC) had a range of value of 0.2 – 4.0 g/dL with an average of 1.6 g/dL. The result of the paired t-test Mann-Witney obtains a p value of 0,000 so that it can be decided that there is a significant difference between the results of post-transfusion hemoglobin level of Whole Blood (WB) and Packed Red Cells (PRC). It is concluded that the post-transfusion hemoglobin level of Packed Red Cells (PRC) is higher than Whole Blood (WB), so it is recommended to use Packed Red Cells (PRC) component to increase hemoglobin levels. Keywords: hemoglobin; transfusion; sectio caesarea; whole blood cells; packed red cells
Penerapan Model Lean Six Sigma Dalam Menurunkan Waktu Tunggu Layanan Pra-Analitik Di Laboratorium Rumah Sakit Fatmawati Jakarta Inderiati, Dewi; Hidayat, Danni; Utamai, Lidya; Anhar, Citra Amaniah
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 11 No. 1 (2025): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v11i1.2619

Abstract

Pre-analytical errors represent a major source of inaccuracy in laboratory testing, contributing to approximately 70% of total diagnostic errors. At Fatmawati Hospital, prolonged patient waiting times within the laboratory workflow have emerged as a critical issue, adversely affecting both service quality and patient satisfaction. Lean Six Sigma (LSS) provides a structured methodology to systematically identify, analyze, and eliminate process inefficiencies and errors. This study aimed to apply the LSS framework to enhance the operational efficiency of pre-analytical laboratory services. An action research design was employed, utilizing the DMAIC (Define, Measure, Analyze, Improve, Control) model. Data collection methods included direct observation, structured interviews, and process audits, with subsequent analysis conducted using descriptive and inferential statistics. Implementation of LSS led to a statistically significant reduction in patient waiting times, decreasing from a mean of 129.13 minutes to 83.04 minutes — an improvement of 46.09 minutes or approximately 35.7%. This outcome was achieved through the elimination of non-value-added activities and the optimization of service pathways. In summary, the findings demonstrate that Lean Six Sigma is an effective approach for improving efficiency and service quality within pre-analytical laboratory operations. The results underscore the potential of LSS to serve as a transformative tool in laboratory process management.  Keywords: Lean Six Sigma, Waiting Time, Pre-analytical Process, Laboratory Services  

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