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Bioscientist : Jurnal Ilmiah Biologi
Published by Universitas Pendidikan Mandalika
ISSN : 23385006     EISSN : 26544571     DOI : https://doi.org/10.33394
Core Subject : Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences
Bioscientist : Jurnal Ilmiah Biologi (BJIB), receiving and publishing article in the form of research (scientific article) in the field of biology education and utilization of biological research in learning. Moreover, this journal bridges the gap between research and practice, providing information, ideas and opinion, in addition to critical examinations of biology research and teaching. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. Special emphasize are as follow: 1. Research on Learning Biology (Biology Learning Materials at All Education Levels). 2. Pure Research of Biology Developed or Studied to the Sources, Materials, or Instructional Media Biology (Biology Learning Materials at All Education Levels and Application in Society). 3. Curriculum of Biology Education at all Education Levels. 4. Class Action Research (CAR) and Lesson Study in Biology. 5. Other Qualitative Research of Biology Education. 6. The School Management and Biology/Science Laboratory Management. 7. Biology Learning Evaluation. 8. Teacher Professional Issues/Trends in Biology Education. 9. Another Study for the Scope of Biology Education. 10. Research on Environmental Education.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 75 Documents
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Search results for , issue "Vol. 13 No. 4 (2025): December" : 75 Documents clear
Pengaruh Aplikasi Trichoderma sp. dan Pupuk Organik Kotoran Ayam Terhadap Pertumbuhan Bawang Merah (Allium Ascolonicum L) Nababan, Nurmaini; Rahmadina
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18268

Abstract

This study aims to determine the effect of chicken manure and Trichoderma sp. on the growth and health of shallots (Allium ascalonicum L.). The study was conducted in June–July 2025 at the Greenhouse of the State Islamic University of North Sumatra using a factorial randomized block design (RAK) with two factors. The first factor was the dose of chicken manure (P0 = 0 g, P1 = 100 g, P2 = 120 g, P3 = 140 g), and the second factor was the dose of Trichoderma sp. (T0 = 0 g, T1 = 25 g, T2 = 30 g, T3 = 35 g). The two factors were combined into 16 treatments with three replicates. The parameters observed included plant height, number of leaves, number of wilted leaves, root length, fresh weight, and dry weight of plants. The data were analyzed using ANOVA and followed by DMRT test at the 5% level. The results showed that chicken manure and Trichoderma sp. had a significant effect on plant height, number of leaves, fresh weight, dry weight, and were able to suppress leaf wilting symptoms, but had no significant effect on root length. The combination of treatments yielded the best results compared to single treatments, especially at chicken manure doses P2–P3 and Trichoderma sp. T3. Thus, the application of chicken manure combined with Trichoderma sp. has the potential to optimize shallot growth in an environmentally friendly manner.
Optimasi Kombinasi Ramuan Etnomedisin Penumbuh Rambut Nusa Tenggara Timur Menggunakan Orthogonal Stimulus Response Method Pada Kepadatan Rambut Mencit (Mus musculus) Taek, Maximus M.; Megawati, Dewi Sinta; Subiyakto, Erlangga Sufi; Khoirunnisa, Zahra; Maulina, Novia; Muslikh, Faisal Akhmal; Ma’arif, Burhan
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18301

Abstract

This study aimed to optimize a traditional ethnomedicinal formulation from East Nusa Tenggara (RENTT) as a natural hair-growth candidate and to evaluate its antioxidant activity and biological effectiveness using an alopecia model. A true experimental design was applied using the Taguchi–Orthogonal Stimulus Response Method (OSRM) to determine the optimal combination of five plant extracts, namely Aleurites moluccanus, Hibiscus rosa-sinensis, Ficus benjamina, Polyscias scutellaria, and Hibiscus tiliaceus. In vitro optimization was conducted using the DPPH radical scavenging assay with a Taguchi L27 orthogonal array design. The optimal formulation was subsequently evaluated in vivo using male mice (Mus musculus) with croton oil–induced alopecia. Hair growth effectiveness was assessed based on hair density percentage through histomorphometric analysis with hematoxylin–eosin staining. The results demonstrated that the optimal RENTT formulation exhibited very strong antioxidant activity with an IC₅₀ value of 27.46 ppm. Taguchi analysis identified F. benjamina as the most influential factor in determining formulation performance. In vivo evaluation showed that the RENTT-treated group achieved the highest hair density (49.45 ± 4.70), which was significantly higher than that of the positive control, negative control, and normal control groups. In conclusion, the optimized RENTT formulation demonstrates strong antioxidant properties and a significant stimulatory effect on hair growth, indicating its promising potential as a natural cosmeceutical product based on the local wisdom of East Nusa Tenggara.
Integrasi Metode Fitoremidiasi dan Filtrasi pada reaktor Portabel untuk Pengolahan Air Limbah Gelondongan Mengandung Merkuri di Sekitar Danau Lebo, Sumbawa Barat Rahman, Abdul; Puryadi; Ulfa, Andi Maria
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18310

Abstract

This study aims to design and test the effectiveness of a portable reactor based on filtration and phytoremediation in reducing mercury levels in gold processing wastewater. The study was conducted experimentally using a portable reactor with a water lettuce (Pistia stratiotes) phytoremediation unit and a filtration unit made of biofoam, zeolite rock, and activated carbon. Testing was carried out on contact time variations of 0–120 minutes and repeated use up to 15 times, with mercury level analysis using AAS. The results showed that the reactor was able to reduce mercury levels from 0.7095 mg/L to 0.0040–0.0039 mg/L at a contact time of 90–120 minutes with an efficiency of up to 99.45%, thus meeting the applicable quality standards. The minimum contact time for the reactor to work optimally was 90 minutes. In repeated use, the highest effectiveness occurs in the initial stage, then decreases due to saturation of the filtration media. This portable reactor is effective and has the potential to be applied directly at PETI sites as a practical alternative technology for mercury waste treatment.
Pengaruh Media Tanam Terhadap Pertumbuhan dan Hasil Microgreen Mustard (Brassica juncea L.) Kurnia, Theresa Dwi; Fajar, Isvan
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18325

Abstract

This study aims to determine the effect of growing media on the growth, yield, and quality of mustard microgreens, as well as to determine the best growing media for cultivating mustard microgreens. The study was conducted from March to April 2024. This study was conducted in a greenhouse located at the Science Techno Park, Faculty of Agriculture and Business, UKSW Salatiga, Central Java, at an altitude of 1050 meters above sea level. This study was designed using a randomized block design (RBD) with 5 treatments and repeated 5 times. The growing media treatments consisted of A1 = soil, A2 = sponge, A3 = rockwool, A4 = rice husk charcoal, and A5 = cocopeat. The parameters observed included plant height, number of leaves, dry weight of plants, chlorophyll content, and vitamin C. The data from the study were analyzed using ANOVA at a significance level of 5% and further tested with the BNJ test at 5%. The results showed that rice husk charcoal and cocopeat media had a significant effect on plant height, while soil media had a significant effect on microgreen dry weight. The quality of microgreens based on chlorophyll and vitamin C content is not affected by the growing medium used. The conclusion of this study is that the use of rice husk charcoal and cocopeat as growing media for mustard microgreens cultivation can produce the best results and quality.
Pengaruh Pemberian Ekstrak Etil Asetat Buah Biwa (Eriobotrya japonica (Tunb.) Lindl) Terhadap Profil Mikrobiota Usus Tikus Yang Diinduksi Escherichia coli Lusiana, Diana Intan Gabriella; Hartanti, Yesiska Kristina; Andareas, Pangeran; Renjaan, Justina Andrea
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18372

Abstract

This study investigated the therapeutic potential of ethyl acetate extract derived from Biwa fruit on the gut microbiota profile of E. coli-infected mice. This study used 30 BALB-C mice divided into five groups: positive control (Ciprofloxacin and Lomperamide 130 mg/kgBW), negative control (CMC-Na), and three Biwa extract treatment groups at doses of 200, 400, and 600 mg/kgBW. The findings demonstrated that Biwa extract at 200 mg/kgBW exhibited significant efficacy from day one, resulting in complete suppression of E. coli growth in mice. However, the cohort receiving 400 mg/kgBW showed recurring E. coli growth on day two, potentially attributable to contamination. This research provides promising preliminary evidence of the potential of Biwa fruit as an alternative herbal-based prebiotic therapy to overcome E. coli infection.
Laju Pertumbuhan, Biomassa, dan Produksi Fikosianin Arthrospira platensis Perairan Pulau Jawa Pada Variasi Salinitas Lestari, Mutia Dinda; Sajidan; Pangastuti, Artini
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18387

Abstract

This study aims to identify and determine the optimal salinity that can produce specific growth rates, maximum dry biomass production, and the highest phycocyanin content and productivity in local strains of A. platensis in the waters of Java Island. This study was conducted using three strains of Arthrospira platensis originating from Jepara, Bogor, and Yogyakarta with salinity variations of 10, 20, and 30 ppt and three replicates using walne fertilizer culture media. The cultures were cultivated for eight days to calculate the growth rate and measure the biomass and phycocyanin concentration. The results of this study showed that the biomass of A. platensis in all strains was maximum at 30 ppt. The specific growth rate of A. platensis strains JPR and BGR was fastest at 30 ppt, and phycocyanin production was highest at 20 ppt. Meanwhile, the MRP strain showed a fast growth rate at 10 ppt and the highest phycocyanin production at 30 ppt. Based on the results of this study, it can be concluded that each strain has a different strategy in responding to salinity in terms of growth rate, biomass, and phycocyanin production.
Ekowisata Mangrove Lembar Selatan: Tinjauan Sosial Ekonomi dan Pemanfaatan Ekologis dalam Mendukung Sustainable Circular Economy dan Pembelajaran IPA Kontekstual Gani, Abdul; Arifin, Ahmad Aris; Fauzi, Ahmad; Yustiqvar, Muhammad; Idrus, Agil Al; Jufri, A. Wahab
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18407

Abstract

This study aims to analyze the ecological utilization of mangroves, examine the socio-economic aspects of the local community, and assess the relationship between these uses, conservation efforts, and the educational potential of the area. The urgency of the research lies in the need to integrate ecological utilization, socio-economic sustainability, and the application of a Sustainable Circular Economy, while simultaneously strengthening the role of mangrove ecotourism areas as sources of contextual science learning. The research employed a descriptive approach with data collected through structured in-depth interviews and questionnaires administered to 10 respondents. Data were analyzed using a mixed method, consisting of qualitative descriptive analysis for interview results and quantitative descriptive analysis for questionnaire data. The findings indicate that mangrove utilization is mainly limited to the collection of dead wood due to local regulations, while the exploitation of fauna (mollusks, crabs, and fish) is more dominant as a source of income. Community participation in conservation is relatively high, but knowledge regarding the medicinal benefits of mangroves remains low. These results highlight the need to strengthen sustainable, circular-economy-based utilization and to optimize the area as a source of contextual science learning.
Karakterisasi Senyawa Pestisida pada Nanopartikel Ekstrak Bawang Putih (Allium Sativum L.) dan Uji Mortalitas Terhadap Ulat Grayak (Spodoptera Litura F.) di Laboratorium Josua, Wahyu Ramadhani Putra; Windriyanti, Wiwin; Kusuma, Ramadhani Mahendra
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18441

Abstract

This study aims to characterize the pesticide compounds in garlic (Allium sativum L.) extract nanoparticles and test their mortality effectiveness against armyworms (Spodoptera litura F.) in the laboratory. Nanoparticle synthesis was carried out using the ionic gelation method with chitosan-TPP, which was subsequently characterized using PSA, SEM, FT-IR, and GC-MS. The characterization results showed a particle size of 930.52 nm with a homogeneous distribution (PDI 0.147). GC-MS analysis successfully identified the main bioactive organosulfur compounds, namely diallyl disulfide (20.72%), diallyl trisulfide (14.16%), and diallyl tetrasulfide (5.79%). Bioactivity testing using the sandwich method at five concentration variations (0-1%) showed the highest mortality reaching 70% at a 1% concentration. Probit analysis yielded values of LC₅₀ 1.116% and LC₉₀ 1.720%, as well as LT₅₀ 5.117 days and LT₉₀ 9.893 days. Statistical test results indicated a significant difference (p<0.05) between all treatments and the control group. Thus, it can be concluded that the garlic extract nanoparticle formulation has the potential to be an effective nano-bioinsecticide, with a mode of action that is dose-dependent and time-dependent in controlling S. litura larvae.
Potency Nannochloropsis sp. As An Agent Bioremediation of Lead Heavy Metal (Pb) In Rivers Jenes Shafira, Riannisa’a; Choirunnisa , Dhiya’; Pratiwi, Endah; Saputra, Alanindra
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18454

Abstract

The study aims to test the bioremediation potential of Nannochloropsis sp. against Pb (lead) contamination in the batik textile industrial area. The steps in this study include collecting river water samples from 3 different stations, preparing the samples by adding HNO3, culturing Nannochloropsis sp. seed for 7 days, testing the initial Pb metal content before and after treatment with Nannochloropsis sp. using wet-digestion techniques, and analyzing the samples. The water samples were taken from upstream, midstream, and downstream. The water samples were then preserved with HNO3. After that, Nannochloropsis sp. cultures were conducted, and results showed an increase in algal population after 7 days. Subsequently, metal content testing was conducted using AAS (Atomic Absorption Spectrophotometer) with a wet-digestion technique to eliminate organic substances in the samples from each station. Metal content testing was conducted before and after treatment with algae. The results of this study showed a decrease in Pb metal levels at each station in the Jenes River after treatment with algae and 7 days of culture. The average decrease obtained was 14.0 before treatment and 5.0 after treatment. Nannochloropsis sp. is an important bioremediation agent for degrading Pb (lead) contamination in river water resulting from excessive industrial waste. These findings contribute to the development of waste treatment to preserve the river ecosystem.
Analisis Metabarcoding ITS Plastifer dari Tempat Pembuangan Akhir Bukit Meusara Jantho Aceh Besar: Upaya Mitigasi Orangutan Sumatra (Pongo abelii) dari Risiko Cemaran Mikroplastik Napisyah, Pingkan; Lubis, Syafrina Sari
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18468

Abstract

This study aims to identify the diversity of plastic-colonizing fungi (plastisphere) at the Bukit Meusara Landfill in Jantho, Aceh Besar, located 5-8 km from the Sumatran Orangutan (Pongo abelii) rehabilitation center, using a metabarcoding approach with the Nanopore platform. Analysis results revealed 143 OTUs from 81,414 sequences with a Shannon index of 2.77, indicating high fungal diversity in the plastisphere. The fungal community was dominated by Ascomycota (83.2%), particularly the class Sordariomycetes (51.7%). Dominant species included Mycothermus thermophilus (9-10%), M. thermophiloides (6%), Brocchiosphaera bulbiformis (5%), and Thermomyces lanuginosus (4%). The identified thermophilic fungi are known to produce enzymes such as laccase, cutinase, lignin peroxidase, and manganese peroxidase, which have potential for degrading PET, PS plastics, and biodegradable polymers. This study provides baseline data on indigenous plastisphere diversity that can be developed as a bioremediation consortium for mitigating microplastic pollution in Sumatran orangutan conservation areas.

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