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Bioscientist : Jurnal Ilmiah Biologi
Published by Universitas Pendidikan Mandalika
ISSN : 23385006     EISSN : 26544571     DOI : https://doi.org/10.33394
Core Subject : Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences
Bioscientist : Jurnal Ilmiah Biologi (BJIB), receiving and publishing article in the form of research (scientific article) in the field of biology education and utilization of biological research in learning. Moreover, this journal bridges the gap between research and practice, providing information, ideas and opinion, in addition to critical examinations of biology research and teaching. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. Special emphasize are as follow: 1. Research on Learning Biology (Biology Learning Materials at All Education Levels). 2. Pure Research of Biology Developed or Studied to the Sources, Materials, or Instructional Media Biology (Biology Learning Materials at All Education Levels and Application in Society). 3. Curriculum of Biology Education at all Education Levels. 4. Class Action Research (CAR) and Lesson Study in Biology. 5. Other Qualitative Research of Biology Education. 6. The School Management and Biology/Science Laboratory Management. 7. Biology Learning Evaluation. 8. Teacher Professional Issues/Trends in Biology Education. 9. Another Study for the Scope of Biology Education. 10. Research on Environmental Education.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 1,428 Documents
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Induksi Akar Anggrek Dendrobium lasianthera dengan Pemberian Beberapa Konsentrasi Naphthalene Acetic Acid (NAA) Secara In Vitro Arli, Naura Muthiah; Noli, Zozy Aneloi
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9289

Abstract

Dendrobium lasinthera is an orchid that has considerable potential as a raw material for anti-cancer drugs and for the production of cut flowers. Therefore, this orchid is a plant with high economic value. This orchid has beautiful and long-lasting flowers. The demand for this orchid is increasing along with its diverse uses, so the propagation of this orchid needs to be increased. In vitro propagation is the most appropriate step to reproduce this orchid. One of the stages that determines the success of orchid propagation through tissue culture is the root induction stage before acclimatization. Using the right type and concentration of Growth Regulatory Substances (ZPT) is very important. This research aims to determine the effect of several NAA Growth Regulatory Substance (ZPT) concentrations and determine the best NAA concentration for Dendrobium lasianthera root induction. This study used a Completely Randomized Design (CRD) with several NAA concentrations (Control (A); 0.5 mg/L (B), 1 mg/L (C), and 1.5 mg/L (D)), on Murashige & Skoog (MS) media. The results of this study show that NAA has a significant influence on the percentage of root formation, root emergence time, and root length in the Dendrobium lasianthera orchid. NAA 1.5 mg/L is the best concentration for induction of Dendrobium lasianthera orchid roots.
Penurunan Aktivitas Biofilm Strain MRSA 22156 oleh Tanaman Saga (Abrus precatorius L.) Mutmainnah, Bq.; Ni’matuzahroh, Ni’matuzahroh
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9297

Abstract

The aim of this research is to determine the effect of using saga extract on bacterial biofilm growth, and to determine the most appropriate dose of saga extract in reducing MRSA 22156 biofilm activity. This research uses the Dowd method to determine the total flavonoid content of A. precatorius L leaves. Treatment reduction in MRSA 22156 biofilm growth using the Plate Biofilm Assay method with three repetitions. The results of the research show that total flavonoids can reduce the growth of MRSA 22156 biofilms. The use of total flavonoids from A. precatorius L. leaves in concentrations of 800 mgL-1 to 25 mgL-1 has been proven to have an effect on biofilm growth activity as indicated by the reduction in MRSA 22156 biofilm growth by 67.9% and 25%. The ethanol extract of A. precatorius L. has anti-biofilm activity in reducing the growth of Staphylococcus aureus bacteria that are resistant to Methicillin.
Desain Lembar Kerja Praktikum Berbasis Masalah untuk Menunjang Keterampilan Proses Sains (KPS) pada Materi Kualitas Perairan Amizera, Susy; Destiansari, Elvira; Santri, Didi Jaya; Santoso, Lucia Maria
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9303

Abstract

Industrial activities can increase waste discharge which has an impact on reducing water quality. Decreasing water quality requires methods for solving problems. The development of problem solving abilities can be improved in practical activities. In practical learning, students are expected to be able to reconstruct concepts through solving problems, so they can develop science process skills. These practical activities can be facilitated using problem-based practical worksheets. The aim of this research is to design problem-based practicum worksheets to support students' science process skills related to preliminary studies, product development design, validity testing, and student responses to small-scale testing. The type of research used is development research using the Borg & Gall model to small scale trials. This research was carried out from July to August 2023. The data collection techniques used in this research were interviews and giving questionnaires. The instruments used in this research were an interview guide sheet, a questionnaire sheet for product validation, and a student response questionnaire sheet. Next, the data was analyzed descriptively. Based on the research results, it can be seen that the practicum worksheet that was developed began with a preliminary study regarding the need for problem-based practicum worksheets on water quality material. The design developed is based on problem-based practical worksheet components that have been validated by two experts. Furthermore, a small-scale trial was carried out which showed that the material aspects were categorized as very good, the graphics were categorized as good, and the language was categorized as very good. Thus, the results of this development can be continued in large-scale tests and can be implemented in the practical learning process.
Pengaruh Larutan Hara dan Eco Enzyme terhadap Pertumbuhan Sawi Hijau (Brassica juncea L.) pada Sistem Hidroponik Jasmine, Nataya Annisa; Rachmawati, Diah
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9312

Abstract

Limited agricultural land has caused a decline in the productivity of green mustard (Brassica juncea L.) as one of the nutritious vegetables that is widely consumed by the public. Cultivating vegetables using a hydroponic wick system is a solution to increase the productivity of green mustard. The expensive price of nutrient solutions can be combined with eco enzymes as a provider of essential nutrients for plants. This research aims to determine the effect of the combination of AB mix with eco enzyme on the growth of green mustard. The research was designed with RAL, 8 treatments, and 3 replications. Treatments include A1E0=AB mix 100%+0mL/L eco enzyme, A1E1=AB mix 100%+1mL/L eco enzyme, A1E2=AB mix 100%+2mL/L eco enzyme, A1E3=AB mix 100%+3mL eco enzyme, A2E0=AB mix 75%+0mL eco enzyme, A2E1=AB mix 75%+1mL/L eco enzyme, A2E2=AB mix 75%+2mL/L eco enzyme, and A2E3=AB mix 75%+3mL/L eco enzyme. The parameters measured were plant height, number of leaves, wet weight of roots and shoots, dry weight of roots and shoots, and leaf area. Data were analyzed using analysis of variance and DMRT testing with α=0.05. The combination of AB mix with eco enzyme had no effect on plant height, number and area of leaves, and wet weight of roots and shoots. However, the highest dry weight of roots and shoots in the A2E2 treatment showed significantly different results from the control treatment (A2E0). Eco enzymes act as nutrient substitutes which are influenced by sunlight, plant metabolism and water absorption capacity.
Deskripsi Kemampuan Komunikatif dan Kolaboratif Mahasiswa Pendidikan Biologi Melalui Penerapan Model Pembelajaran Project Based Learning Titin, Titin; Wahyuni, Eko Sri; Komaria, Siti; Ayu, Nambisas Arum Kusuma; Wilma, Wilma
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9318

Abstract

This research was conducted using descriptive research methods. This method was chosen to describe the communicative and collaborative abilities of Biology Education students through the application of the Project Based Learning (PjBL) model. The research stages are adapted to a model consisting of 6 stages, namely determining the project, planning steps to complete the project, preparing a schedule for implementing the project schedule, implementing the project with lecturer monitoring, preparing reports and publishing project results, and evaluating the implementation of activities, as well as the final results of the project. The research instrument used in this research is a questionnaire to measure communicative and collaborative abilities which was completed by 64 Biology Education student respondents, Faculty of Teacher Training and Education, Tanjungpura University. The results of the analysis of the communicative questionnaire which is composed of 6 aspects, obtained aspects of task presentation 88% in the very good category, asking questions 58% in the sufficient category, responding to presentation results 92% in the very good category, discussion 89% in the very good category, cooperation in groups 89% in the very good category, and decision making 76% in the good category. Meanwhile, the results of the collaborative questionnaire analysis showed that the group contribution aspect was 95% in the very good category, mutual assistance was 64% in the sufficient category, and flexibility was 95% in the very good category. From these results it was found that the average for each communicative and collaborative questionnaire was respectively 76% in the good category and 84.7% in the very good category. So it can be concluded that the application of the Project Based Learning (PjBL) model can improve students' communicative and collaborative abilities in the learning process.
Uji Aktivitas Sel Kanker Payudara T47D Ekstrak Etil Asetat Daun Glodokan Tiang (Polyalthia longifolia L.) Secara In Vitro Shofi, Muh.; Munawaroh, Siti
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9345

Abstract

Cancer is one of the main causes of death throughout the world. Breast cancer is a non-communicable disease and a type of cancer that is often suffered by women. Breast cancer is a reproductive health problem, both in the world and in Indonesia, which is now a serious concern. Medical treatment of cancer is usually carried out with chemotherapy, surgery and radiotherapy, but the Indonesian people are familiar with and use efficacious plants as an effort to cure various diseases, including cancer. One of them is by using glodokan mast leaves or Polyalthia longifolia L. as natural chemotherapy from biological ingredients. This plant is reported to be high in antioxidant content, so it can be used as an anticancer agent, especially T47D breast cancer. The aim of this research is to examine the compound content and activity of T47D breast cancer cells from P. longifolia leaf extract. This research is basic experimental research, namely by comparing the IC50 value of ethyl acetate extract on young and old leaves. Based on the research results, it is known that the ethyl acetate extract in young and old leaves contains alkaloids, flavonoids, tannins and terpenoids which are useful as anticancer compounds. Cytotoxic activity of ethyl acetate extract of P. longifolia leaves against T47D breast cancer cells with an IC50 value of 253.76 μg/mL on old leaves and young leaves of 220.54 μg/mL with moderate cytotoxicity.
Efektivitas Eco Enzyme Limbah Jeruk Manis (Citrus sinensis) sebagai Obat Oles Penyembuhan Luka Sayat Dzahabiyyah, Qonitah; Asy’ari, Asy’ari; Suharti, Peni
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9346

Abstract

Sweet orange (Citrus sinensis) is a fruit that is much liked by Indonesian people, but from this fruit only the flesh and skin is wasted which eventually becomes waste. Because of that, an alternative solution to this problem is needed, namely Eco Enzyme is made in the form of a multifunctional solution that is used as a wound medicine. The purpose of this study was to determine the differences in the effect of sweet orange (Citrus sinensis) Eco Enzyme concentrations on the healing rate of cuts in mice (Mus musculus). This type of research was experimental with a Completely Randomized Design (CRD) consisting of 7 treatment groups and 4 repetitions, the research data analysis technique used the Kruskal-Wallis and Mann Whitney tests. The results showed that the average percentage of healing rate by applying Eco Enzyme topical medication from sweet orange (Citrus sinensis) waste was most effective on the healing rate of cuts in mice (Mus musculus) with various concentrations, namely P5 (100%) healing rate 100% average healing time 8.25, P4 (80%) healing rate 93.75% average healing time 9.25, P3 (60%) healing rate 87.5% average healing time 9.75, P2 (40%) cure rate 81.25% average healing time 10.25, P1 (20%) healing rate 75% average healing time 10.75, positive control (K+) betadine has a healing rate of 87.5% healing time an average of 10.25, negative control (K-) distilled water has a 50% healing rate healing time beyond day 14. The conclusion obtained is that there is a difference in the effect of orange Eco Enzyme concentration sweet (Citrus sinensis) on the healing rate of cuts in mice (Mus musculus).
Analisis Kesulitan Belajar Siswa Kelas X IPA di SMA Negeri 3 Tebas pada Materi Protista Hisdayu, Hisdayu; Titin, Titin; Tenriawaru, Andi Besse
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9347

Abstract

Biology is considered difficult for students to study, this condition cannot be separated from the nature of science. Studying science is complex because it emphasizes unification, systematization, combination and analysis of information and concepts. The aim of this research is to measure the level of learning difficulties experienced by class X Science students on Protista material. The method used is a case study. The research subjects were students of class X Science at SMA Negeri 3 Tebas, Sambas Regency. Data collection instruments consist of diagnostic tests, questionnaires and interviews. The results of the research showed that the learning difficulty of class X Science students at SMA Negeri 3 Tebas on Protista material was 91.94% with very high criteria. The factors that influence students' learning difficulties are internal factors of 78.18% with strong criteria, and external factors of 64.14% with strong criteria.
Uji Aktivitas Antifungi Kombinasi Ekstrak Etanol Kulit Pisang Kepok (Musa paradisiaca L.) dan Kulit Jeruk Manis (Citrus sinensis) terhadap Candida albicans Penyebab Keputihan Patologis Secara In Vitro Yulia, Inelvi; Prima, Heppy Setya
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9360

Abstract

Pathological vaginal discharge (Candidiasis) is a reproductive health problem experienced by more than 50% of women throughout the world. Candida albicans is the main cause of pathological vaginal discharge. This disease is characterized by yellow to greenish discharge, odor, and itching to the point of bleeding which can cause death. Antifungal drugs used to treat pathological vaginal discharge can have several side effects. Therefore, the urgency of this research is to find natural alternatives for pathological vaginal discharge drugs, such as sweet orange peel and kepok banana peel which contain bioactive compounds that can inhibit the growth of Candida albicans. The aim of this study was to test the antifungal activity of a combination of ethanol extracts of sweet orange peel and kepok banana peel against Candida albicans in vitro. This research uses laboratory experimental methods. The results showed that all combinations of ethanol extracts of sweet orange peel and kepok banana peel could inhibit the growth of Candida albicans. The inhibitory power of each extract combination is categorized as moderate to very strong. According to the one-way ANOVA statistical test, all combinations were significantly different (p < 0.05). The ethanol extract of sweet orange peel contains flavonoids, alkaloids, phenolics and steroids, while the kepok banana peel extract contains phytochemical compounds such as flavonoids, phenolics, triterpenoids and alkaloids.
Respon Pertumbuhan Anggrek Dendrobium Fase Seedling terhadap Variasi Jenis Media Tanam Kismayanti, Choirul Nisa; Ulfah, Maria; Rachmawati, Rivanna Citraning
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9368

Abstract

Orchids are ornamental plants that are very popular with the public and consist of many types of orchids. The aim of this research is to find out how various types of planting media influence the growth of dendrobium orchids. This study was conducted for three months, from May to July 2023. The research used was a Completely Randomized Design (CRD) which consisted of three different types of planting media, namely: M1 (kadaka roots); M2 (wood charcoal); and M3 (Sphagnum moss). Data analysis was carried out using one-way ANOVA and the Lowest Significant Difference (LSD) test at the 5% level. The results of the analysis show that there are differences in the types of planting media used to plant kadaka roots, Sphagnum moss, and wood charcoal. In the planting medium, kadaka roots grow well with high variable stem growth. In the Sphagnum moss planting medium, it gave the best growth in the shoot height increase variable, while in the wood charcoal planting medium it did not show suboptimal growth in terms of stem height, stem diameter, shoot height and number of roots. Sphagnum moss planting medium provides the best growth for increasing shoot height.

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