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Bioscientist : Jurnal Ilmiah Biologi
Published by Universitas Pendidikan Mandalika
ISSN : 23385006     EISSN : 26544571     DOI : https://doi.org/10.33394
Core Subject : Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences
Bioscientist : Jurnal Ilmiah Biologi (BJIB), receiving and publishing article in the form of research (scientific article) in the field of biology education and utilization of biological research in learning. Moreover, this journal bridges the gap between research and practice, providing information, ideas and opinion, in addition to critical examinations of biology research and teaching. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. Special emphasize are as follow: 1. Research on Learning Biology (Biology Learning Materials at All Education Levels). 2. Pure Research of Biology Developed or Studied to the Sources, Materials, or Instructional Media Biology (Biology Learning Materials at All Education Levels and Application in Society). 3. Curriculum of Biology Education at all Education Levels. 4. Class Action Research (CAR) and Lesson Study in Biology. 5. Other Qualitative Research of Biology Education. 6. The School Management and Biology/Science Laboratory Management. 7. Biology Learning Evaluation. 8. Teacher Professional Issues/Trends in Biology Education. 9. Another Study for the Scope of Biology Education. 10. Research on Environmental Education.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 1,439 Documents
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Inventarisasi Varietas Tanaman Puring (Codiaeum variegatum (L.) Rumph. ex A. Juss.) di Desa Jeruju Besar Kecamatan Sungai Kakap Kabupaten Kubu Raya Faturrahman, Mas Akhbar; Fadhilah, Azfa; Nufitasari, Nufitasari; Filza, Inne Aqmarina; Fajri, Hayatul
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9425

Abstract

Indonesia is a country that has high biodiversity. One of Indonesia's biodiversity plants is the croton plant (Codiaeum variegatum (L.) Rumph. ex A. Juss.). The Croton plant is one of the plants used by the general public as an ornamental plant because of the various shapes and colors of its leaves. This research aims to carry out an inventory of various varieties of croton plants in Jeruju Besar Village, Sungai Kakap District, Kubu Raya Regency, West Kalimantan Province. This research is a qualitative descriptive study with stages including taking samples of croton plants followed by identifying and providing descriptions of croton plants. The results of the research show that there are 24 varieties of croton based on the leaves color and shape, namely ‘Daylight’, ‘Mentimun’, ‘Gold Star’, ‘Burgundy’, ‘Bor Merah’, ‘Batik Mini’, ‘Mentega’, ‘Kecapi’, ‘Trisula’, ‘Tiger’, ‘Spaghetti Three-Color’, ‘Kura Lokal Variegata’, ‘Emping’, ‘Koi’, ‘Manik Maya’, ‘Jazzy Hybrid’, ‘Lele Kirmizi’, ‘Holland’, ‘Kura Batu’, ‘Jumbo Jet’, ‘Adreanum’, ‘Keris’, ‘Jet Amerika’, and ‘Kelabang’.
Validitas Petunjuk Praktikum Berbasis POE (Predict, Observe, and Explain) Sahratullah, Sahratullah; Seprianingsih, Dewi; Mardiana, Mardiana
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9438

Abstract

This research was carried out with the aim of finding out the validity of POE-based practicum instructions (Predict, Observe and Expline) at SMP Negeri 4 Taliwang. This type of research refers to the 4D development model, which consists of four stages, namely Define, Design, Develop, and Disseminate. This research stage was only carried out until the develop stage, namely testing development on a limited scale. This is because the POE (Predict, Observe and Expline) based practicum instructions have not yet reached the Disseminate stage, namely the dissemination stage at the high school level in West Sumbawa Regency. The instrument used in this research was a validation test sheet for POE-based practicum instructions with data collection techniques using the average expert validation score. The results of data analysis show that the development of a POE-based practical manual is valid and very suitable for use. The validation results obtained very high feasibility from the 10 assessment indicators with a value of 87.5%. POE-based practical instructions have been validated by a team of experts, then revised based on suggestions from the validator. As well as the results of the POE-based practicum instructions readability test on 15 indicators with easy criteria with a score of 98.64%. Among the 15 readability test indicators, there were 2 indicators that received hesitant answers from respondents and 3 indicators that received disagreeing answers from respondents. The implications of these practical instructions can be utilized by educators and students in learning in the classroom and outside the classroom, especially in laboratory activities.
Etnobotani Tumbuhan Obat oleh Masyarakat Desa Sungai Ulu Kabupaten Natuna Suri, Linda Amelia; Syamswisna, Syamswisna; Mardiyyaningsih, Asriah Nurdini
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9440

Abstract

Sungai Ulu Village, East Bunguran District, Natuna Regency is a village where the people use plants as traditional medicine. It is necessary to document medicinal plants in order to increase awareness to preserve medicinal plants. The aim of this research is to conduct an ethnobotanical study of medicinal plants to determine the use of medicinal plants by the people of Sungai Ulu Village. This type of research is qualitative research with descriptive methods, with samples taken using snowball sampling techniques. The data collection technique uses triangulation, namely a combination of interviews, observation and documentation. The research results showed that there were 76 types of medicinal plants used by local communities, consisting of 45 families that could treat 41 types of diseases. The part of the plant that is most widely used is the leaves, while the habitus that is most commonly found is herbs with the most locations in home gardens, with the most processing and use method being boiled and then drunk.
Surveilans Aedes aegypti Menggunakan Ovitrap di Desa Endemis Demam Berdarah Kabupaten Banyumas Wuliandari, Juli Rochmijati; Mulia, Dini Siswani; Susanto, Susanto
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9465

Abstract

Entomological surveillance is essential to evaluate the abundance and distribution of Aedes aegypti mosquito, the dengue virus’s primary vector. This study investigates the abundance and distribution of Aedes spp collected with ovitraps in Mersi and Ledug, Purwokerto Timur, Banyumas. Both localities are identified as dengue endemic areas by the Banyumas’ Health Service. Ovitrap surveillances were performed every two weeks in Mersi and Ledug, in January – March 2022. For each localitiy, 50 respondent houses were randomly selected and 5 ovitrap surveillance trips were conducted. In each trip, 100 ovitraps were distributed to 50 respondent houses. At every single house, one ovitrap was placed inside the house (indoor) and one outside the house (outdoor). All samples collected were transported to and reared at Zoology laboratory, UMP. Mosquito larvae were identified to species at instar IV and totaled. The OPI (Ovitrap positivity index) for both indoor and outdoor ranged from 38,00% - 59,70%. The mean number of larvae for Mersi outdoor (612,50±109,73) was higher than Mersi indoor (478,80±42,96) p= .031 and Ledug indoor (347,20±45,04) p= .000, but not significantly different from Ledug outdoor (507,20±51,07) p= .109. There was no significant difference for EDI (egg density index) in the four ovitrap placements, F(3,16) = 1.132, p= .366. However, the MNE (mean number of eggs) showed a significant difference for Mersi and Ledug both indoor and outdoor F(3,16) = 14.528, p= .000. Conclusion: The OPI showed that the distribution of Aedes spp in Mersi and Ledug was high. Ae. aegypti and Ae. albopictus were found as both outdoor and indoor breeder although Ae. aegypti was found to be the dominant both as indoor and outdoor breeder.
Uji Antagonis Bacillus subtilis ATTC 6633 dan Trichoderma harzianum terhadap Pertumbuhan Magnaphorte oryzae pada Benih Padi Anak Daro dengan Variasi Lama Perendaman Alamsjah, Feskaharny; Noli, Zozy Aneloi; Rahmayati, Riesca Salsabilah; Suwirmen, Suwirmen; Agustien, Anthoni; Ilham, Kurniadi
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9468

Abstract

Magnaphorte oryzae is a pathogenic fungus that causes blast disease in rice plants. This disease can cause a decrease in rice production. Therefore, it is necessary to control the disease, one of which is by administering microbes that are antagonistic to Magnaphorte oryzae. This research aims to determine the percentage of inhibitory power of Bacillus subtilis ATTC 6633 and Trichoderma harzianum on the growth of Magnaphorte oryzae with varying soaking times of 24, 48 and 72 hours on the Anak Daro rice variety. This research carried out viability tests for Bacillus subtilis ATTC 6633 and Trichoderma harzianum, as well as antagonist tests using the dual culture method. The results showed that the growth inhibition of Magnaphorte oryzae by Bacillus subtilis ATTC 6633 for a soaking period of 24 hours was 28.48% in the weak category, 48 hours was 32.27% and 72 hours was 31.01% in the medium category. Inhibition of Magnaphorte oryzae by Trichoderma harzianum for a soaking period of 24 hours was 50.63%, 48 hours was 57.59%, and 72 hours was 56.32% in the high category.
Etnobotani Tumbuhan Obat yang Dijual di Blok Bahan Jamu Pasar Raya I Salatiga Ananda, Raditya Se'o; Suprihati, Suprihati
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9486

Abstract

Ethnobotany is a branch of science that studies the correlation between humans and plants. Generally, the basic ingredients for making herbal medicine come from types of medicinal plants. The majority of city residents buy medicinal plants from markets or drug stores. Pasar Raya I Salatiga as a source of meeting the needs of the people of Salatiga City. The aim of this research is to know the types of medicinal plants being sold, knowing the parts of the medicinal plants being sold, knowing the shape of the medicinal plants being sold, knowing how to obtain the medicinal plants being sold, and knowing the agrotechnological engineering actions of one of the medicinal plants being sold. This research is a qualitative descriptive research. This research was conducted with three herbal medicine sellers, three consumers, and three ginger farmers. This research was conducted in August-September 2023. Data collection techniques used semi-structured interviews, observation, literature study and documentation. Data processing with simple statistics. The research results found a total of 92 medicinal plants. The parts of medicinal plants sold include roots (4%), stems (7%), seeds (19%), fruit (16%), flowers (4%), leaves (26%), leaves & flowers (1%), herbs (1%), bark (4%), rhizomes (15%), and tubers (3%). The forms of medicinal plants sold include dry (71%), fresh (17%), dry & fresh (7%), and processed (5%). All medicinal plants are obtained by purchasing. Agrotechnological engineering actions carried out by ginger farmers include applying fertilizer, planting in the right season and scheduling planting.
Pengaruh Rasio Crude Enzim Selulase Trichoderma reesei dan Aspergillus niger terhadap Kadar Gula dan Bioetanol Fermentasi Kulit Kacang Tanah (Arachis hypogaea L.) Menggunakan Zymomonas mobilis Widyaningrum, Trianik; Rizqiyah, A’isyah Arroobi’atu
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9487

Abstract

The need for fuel oil in Indonesia is increasing along with the increase in the number of people's consumption in the fields of transportation, industry, and economy. While the source of petroleum fuel in Indonesia is running low. Therefore, it is necessary to utilize alternative energy, namely bioethanol. Bioethanol can be produced from organic waste that contains high cellulose, one of which is peanut shells. This study aims to determine the effect of the ratio of crude cellulase enzyme Trichoderma reesei and Aspergillus niger on sugar and ethanol content of peanut shell fermentation using Zymomonas mobilis, and to determine the ratio of crude cellulase enzyme that produces the highest sugar and ethanol content of peanut shell fermentation using Z. mobilis. This research is an experimental study with independent variables, namely the ratio of crude cellulase enzyme T. reesei : A. niger (0:0), (1:0), (0:1), (1:1), (1:2), (2:1), (1:3), and (3:1) and the dependent variable of sugar and biethanol content. Data analysis of experimental research results using ANOVA and DMRT test. The addition of crude cellulase enzyme from T. reesei and A. niger affects the sugar content and bioethanol from peanut shell fermentation. The ratio of crude cellulase enzyme from T. reesei and A. niger that produces the highest sugar content is in the treatment P5 ratio of crude enzyme T. reesei: A. niger (2:1) which is 0.14 g/mL and the highest ethanol content from groundnut shell fermentation is in the treatment of P8 crude enzyme ratio of T. reesei : A. niger (1:3) which is 2.23%.
Pengaruh Konsentrasi Crude Enzim Bacillus subtilis terhadap Kadar Gula dan Bioetanol Hasil Fermentasi Kulit Singkong Menggunakan Zymomonas mobilis Nurusman, Arief Abdillah; Widyaningrum, Trianik; Nisa, Rifda Khairun; Utami, Listiatie Budi
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9491

Abstract

Bioethanol is an environmentally friendly and renewable alternative fuel. The use of alternative fuels is necessary because petroleum fuels make a major contribution to increasing greenhouse gas concentrations, especially CO2. Bioethanol can be obtained from materials containing carbohydrates such as cassava peel which has a high carbohydrate content, namely 4.55%. The aim of this research was to see the effect of the crude enzyme concentration of Bacillus subtilis on the results of sugar and bioethanol content of cassava peel fermentation with Zymomonas mobilis and to find out the crude enzyme concentration that had the greatest influence. This research is an experimental study with the independent variable, namely the concentration of crude cellulase enzyme B. subtilis with concentrations of 0%, 2.5%, 5%, 7.5%, 10%, 12.5%, 15%, and 17.5% , and the dependent variables are sugar content from hydrolysis and bioethanol content from the fermentation process. The data obtained were analyzed using the ANOVA test and Duncan's test. The results of research on the effect of crude Bacillus subtilis cellulase enzyme concentrations of 8 concentrations have an influence on the amount of sugar and bioethanol produced from fermentation of cassava peel with Zymomonas mobilis. Crude Bacillus subtilis cellulase enzyme with a concentration of 17.5% had the greatest influence on sugar and bioethanol levels with a sugar content of 0.37% and a bioethanol content of 1.67%.
Pengembangan Protokol Deteksi Staphylococcus aureus Berbasis Molekuler Koentjoro, Maharani Pertiwi; Alviani, Melinda Nuril; Jatmiko, Yoga Dwi; Habibah, Laila Nur; Al Fatih, Ahmad Nuril Fuad; Kartikaningsih, Hartati
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9494

Abstract

Staphylococcus aureus becomes a normal flora in humans, especially on the skin and in the nose. However, if it becomes excessive or if there are pathogenic variants, it can cause various health problems. The purpose of the study is to develop a molecular-based detection method for Staphylococcus aureus using the norA primer gene. The norA gene in Staphylococcus aureus is known to play a role in pathogenesis with its antibiotic resistance ability. This type of research is analytical observational with a cross-sectional method. The methods in the study include the isolation and identification of Staphylococcus aureus from healthcare facility equipment. Isolation and identification include bacterial isolation using Blood Agar Plate (BAP) media; isolate purification, Gram staining; biochemical tests using Mannitol Salt Agar (MSA) media, glucose tests, Voges Proskauer (VP) tests, catalase tests, and coagulase tests. Furthermore, S. aureus isolates were tested using a molecular-based method, namely Polymerase Chain Reaction (PCR). This method includes DNA isolation stages, qualitative testing with agarose gel electrophoresis, semi-quantitative testing with image J software, amplification with Real-Time Polymerase Chain Reaction (RT-PCR) using norA gene primers. The Mann-Whitney test results gave a value of p = 0.334 (p> 0.05) indicating the suitability between the culture method and the PCR method with the developed protocol in detecting Staphylococcus aureus. The developed method includes the use of base sequences in the norA gene primer, optimization of annealing and extension temperatures, and the concentration of DNA templates used.
Meta-Analisis : Efektivitas Multimedia Interaktif terhadap Peningkatkan Hasil Belajar Siswa Khaeruman, Khaeruman; Sudiatmika, AAIA Rai; Suma, I Ketut; Suardana, I Nyoman
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9497

Abstract

This research aims to determine the effectiveness of interactive multimedia on student learning outcomes by analyzing 15 articles that can be accessed from Google Scholar, Garuda, and Mendeley. These articles were published in non-accredited journals, accredited Sinta, and indexed by Copernicus or Scopus with 5 different sample levels, namely: Elementary School, Middle School, High School, Vocational High School, and College. The use of interactive multimedia contains text, graphics, audio and moving images (video) which can be displayed via projector and laptop screens. This research method uses a meta-analysis method, namely by comparing research data from control and experimental groups in the form of sample size (N), Standard Deviation (SD), and learning outcomes (X). The data was analyzed statistically to see the effect size value. Based on the results of the analysis test, it was found that from the 15 articles analyzed, the average effect size was 1.146 with a P-Value < 0.001 (P-Value < 0.05), which means that learning using multimedia is very effective or has an influence on student learning outcomes. Next, to see at which level the effectiveness value is higher, the analysis results found that the estimated value at the elementary school level was 1.719 (more effective), followed by the tertiary level with an estimate of 1.707.

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