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Agung Suharyanto
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suharyantoagung@gmail.com
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+628126493527
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mrc@mahesacenter.org
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Griya Nafisa 2, Bandar Khalipah, Deli Serdang, Sumatera Utara
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INDONESIA
Journal of Natural Sciences
Published by Mahesa Research Center
ISSN : -     EISSN : 27215571     DOI : 10.34007
Core Subject : Agriculture, Social,
The Journal of Natural Sciences is a scientific publication media in the study and development of Science in the fields of science and Education (Biology, Chemistry, and Physics). The Journal of Natural Sciences includes scientific writings in the form of conceptual, research results as well as relevant issues in the fields of science and education. The Journal of Natural Sciences is published three times a year.
Articles 117 Documents
Peran Ekosistem Laut dalam Konservasi Keanekaragaman Hayati Di Indonesia Salayan, Lia Mandalika; Wulandari, Heni; Huda, Muhammad Komarul
Journal of Natural Sciences Vol 5, No 3 (2024): Journal of Natural Sciences November
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v5i3.717

Abstract

Indonesia's marine ecosystems play a crucial role in global biodiversity conservation, with regions rich in coral reefs, mangroves, and seagrass beds. The high marine biodiversity makes Indonesia home to various endemic species. However, these ecosystems face serious threats due to climate change, plastic pollution, overfishing, and coastal habitat degradation. This study aims to identify the role of marine ecosystems in biodiversity conservation in Indonesia, as well as the challenges they face. The research method used in this study includes literature review and analysis of marine conservation policies in Indonesia. The results show that Indonesia's marine ecosystems, including coral reefs, mangroves, and seagrass beds, significantly contribute to the sustainability of biodiversity and ecosystem balance. However, threats from both natural factors and human activities require more intensive conservation efforts. Conservation policies in several areas, such as Raja Ampat, have shown positive results, but there is a need for improvement in law enforcement and international collaboration. Therefore, effective and coordinated conservation efforts are essential to protect Indonesia's marine ecosystems and biodiversity for future generations
Efektivitas Pestisida Berbahan Nimba Terhadap Mortalitas Sitophilus zeamais (Coleoptera: Curculionidae) di Penyimpanan Biji-bijian Purwatiningsih, Purwatiningsih; Alya, Fathia Anisa; Winarsa, Rudju; Muzakhar, Kahar
Journal of Natural Sciences Vol 5, No 3 (2024): Journal of Natural Sciences November
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v5i3.677

Abstract

Sitophilus zeamais is known as a warehouse pest insect that can cause damage to post-harvest crops by 0.5-2%. Effective control by considering food safety is the use of neem-based pesticides. This study aims to determine the effect of neem-based pesticides on S. zeamais mortality. The study was designed with a Completely Randomized Design (CRD) consisting of 6 treatments and 3 replications, with a spraying method. Measurement of feed weight before and after pesticide application was also observed. The results showed that neem-based pesticides had a significant effect on insects (p 0.05). Insect mortality at concentration of 0.5% showed the highest mortality with an average of 24%. Neem-based pesticide application also affected the amount of feed weight consumed by S. zeamais. At concentrations of 0.125%, 0.25% and 0.5%, the weight of feed consumed showed a decrease compared to concentrations of 1%, 2% and control. It can be concluded that pesticides containing active ingredients of neem at a concentration of 0.5% have the potential effect to control the activity of S. zeamais in post-harvest products based on the insect mortality and damage activity, especially on rice seeds.
Pengaruh VCO terhadap Kualitas Plastik Biodegradable Berbasis Selulosa Bakteri Sorbitol dari Air Kelapa Anjaly, Azzahra Nurfadillah; Putra, Ananda
Journal of Natural Sciences Vol 5, No 3 (2024): Journal of Natural Sciences November
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v5i3.699

Abstract

Plastic is a material that is widely used by society today, causing environmental problems, so a solution is needed so that it does not harm the environment, namely biodegradable plastic. This plastic is an environmentally friendly type of plastic because it is easily degraded by microorganisms. This research was carried out in-situ using FTIR and in this research, various VCO volumes of 2 ml, 4 ml, 6 ml and 8 ml were used with the addition of 30% sorbitol as a plasticizer. Based on the test results, it shows an elongation value of 15.08%, an elasticity value of 1264.41 Mpa, and a tensile strength value of 133.98 Mpa with variations in the addition of 8 mL of VCO. This tensile strength value meets the SNI standard for synthetic plastics, namely in the range of 24.7-302 Mpa. The addition of more and more VCO had an effect on the biodegradation test, where on day 15 the plastic had completely decomposed. In the FTIR functional group analysis, no new functional groups were formed, and in the analysis using XRD when adding VCO, the percentage of plastic crystals was greater, namely 98.02%, compared to bacterial cellulose, which was only 97.01%, and bacterial cellulose-sorbitol, which was 95.79%.
Efek Pemberian Ekstrak Daun Salam (Syzygium polyanthum L.) terhadap Parameter Darah Tikus Putih (Rattus norvegicus L.) yang Terpapar Kadmium Klorida (CdCl₂) Ahyadiyani, R.; Tambunan, Efrida Pima Sari; Hutasuhut, Melfa Aisyah
Journal of Natural Sciences Vol 6, No 1 (2025): Journal of Natural Sciences Maret
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v6i1.811

Abstract

Cadmium is a non-essential, carcinogenic metal commonly found in the environment. It can enter the human body through food consumption and has the potential to disrupt physiological systems, including the hematopoietic system. Bay leaves (Syzygium polyanthum) contain various bioactive compounds such as flavonoids, tannins, saponins, alkaloids, and antioxidants, which play a role in neutralizing free radicals. This study aims to analyze the effect of ethanol extract of bay leaves on erythrocyte, leukocyte, platelet counts, hemoglobin levels, and hematocrit in white rats (Rattus norvegicus L.) exposed to cadmium chloride (CdCl₂). The study employed a completely randomized design (CRD) with 20 rats divided into five treatment groups: a negative control, a positive control (CdCl₂ 40 mg/kgBW), and three treatment groups receiving bay leaf extract at doses of 200, 300, and 400 mg/kgBW. The research procedures included phytochemical screening, antioxidant activity testing, and blood profile analysis. The obtained data were analyzed using one-way ANOVA, followed by Duncan's post hoc test if significant differences were found. The results showed that administration of ethanol extract of bay leaves was able to restore blood parameters in rats to normal levels, with an optimal dose of 400 mg/kgBW.
Aspek Etika dalam Pembuatan Nutrisi Hidroponik untuk Kangkung (Ipomoea aquatica) Nurkhofifah, Indah; Sastra, Siti Fathiya Dwinda; Husniatunnisa, Husniatunnisa; Lubis, Khairiza; Situmorang, Nurbaity
Journal of Natural Sciences Vol 6, No 1 (2025): Journal of Natural Sciences Maret
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v6i1.715

Abstract

Water spinach (Ipomoea aquatica) is a nutritious vegetable and has great potential in hydroponic cultivation. However, the creation of appropriate and ethical nutrients for the growth of this plant is still a challenge. This study aims to analyze the application of ethics in the creation and optimal concentration of nutrients A and B needed by water spinach plants. The method used is an experimental approach at the Horticultural Seed Development Unit, Medan, during the period January to March 2025. Data collected include plant height, number of leaves, and fresh weight of plants. The results of the study indicate that the right concentration of nutrients can increase the growth of water spinach plants and ensure the safety of consumption. This study provides insight for farmers and hydroponic practitioners in improving the quality of water spinach cultivation.
Analisis Kadar Kreatinin pada Laki-Laki Pecandu Kopi di Dusun IV Desa Saentis Qibetya, Ananda Nurul; Suharsih, Suharsih; Parinduri, Junaidi; Tamara, Chatrine Virginia
Journal of Natural Sciences Vol 6, No 1 (2025): Journal of Natural Sciences Maret
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v6i1.804

Abstract

Coffee consumption has become a part of people's lifestyles, especially among men. The main component of coffee is caffeine, which has a stimulating effect on the central nervous system. However, excessive consumption can lead to kidney function disorders, which can be identified through increased serum creatinine levels. This study aims to describe creatinine levels in male coffee addicts in Dusun IV, Saentis Village, Percut Sei Tuan District. This research employs a descriptive observational method with a cross-sectional approach. A total of 25 male coffee addicts had their serum creatinine levels measured using the enzymatic method with the Indiko Plus Analyzer. The results showed that the average creatinine level was 1.72 g/dL, with the lowest at 0.77 g/dL and the highest at 2.01 mg/dL. A total of 28% of respondents had high creatinine levels, while 72% were within the normal range. Respondents aged ≥35 years tended to have higher creatinine levels compared to those under 35 years old. Coffee consumption of ≥5 cups per day was associated with increased creatinine levels. This study indicates that excessive coffee consumption may pose a risk to kidney health.
Analisis Perbedaan Kadar Ureum dan Kreatinin dalam Serum dan Plasma pada Pasien dengan Gagal Ginjal di RSU Indah Bagan Batu Rozi, Sihab Fahrul; Suharsih, Suharsih; Febriantika, Febriantika; Parinduri, Junaidi
Journal of Natural Sciences Vol 6, No 1 (2025): Journal of Natural Sciences Maret
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v6i1.806

Abstract

Urea and creatinine serve as key indicators in the evaluation of kidney function. Measurement of urea and creatinine levels can be performed using either serum or plasma. This study aims to compare the results of urea and creatinine level examinations obtained from serum and plasma in patients with kidney failure at RSU Indah Bagan Batu. The research method used is descriptive observational with a cross-sectional approach. The study sample consisted of 22 kidney failure patients who underwent urea and creatinine level examinations using an enzymatic method with an automatic analyzer. The results showed that the average urea level in serum was 186.7 mg/dL, while in plasma, it was 184.9 mg/dL, with a p-value of 0.917 (p 0.05), indicating no significant difference. Similarly, the average creatinine level in serum was 14.17 mg/dL, while in plasma, it was 14.15 mg/dL, with a p-value of 0.988 (p 0.05). Based on these findings, it can be concluded that urea and creatinine level examinations can be conducted using either serum or plasma without significant differences in the results.
Identifikasi Bakteri Endofit Pada Daun Sirih (Piper betle L.) Dengan Metode 16S rRNA Prasetya, Eko; Damanik, Joshua Leonard
Journal of Natural Sciences Vol 6, No 1 (2025): Journal of Natural Sciences Maret
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v6i1.778

Abstract

Betel leaf (Piper betle L.) is a plant that has antibacterial properties, and is one of the plants that can be used as a source of endophytic bacteria. Endophytic bacteria can produce the same secondary metabolites as the host plant. In identifying bacteria, the 16S rRNA gene is used This study aims to identify endophytic bacterial species that were successfully isolated from betel leaves using the 16S rRNA method.The betel leaves taken are old leaves. The methods used are laboratory research methods and literature studies. Laboratory methods include isolation and molecular identification of endophytic bacteria. The literature study method is to look for previous research results in the form of journals, books, articles and compare them with the laboratory isolation results obtained. The results of molecular identification of endophytic bacteria have 99.81% similarity to Lysinibacillus fusiformis strain N169 (IE 1), 100% similarity to Pseudomonas aeruginosa strain PaLo22 (IE 2), and 100% similarity to Bacillus cereus strain Z2-R9 (IE 3). It can be concluded that there are 3 endophytic isolates that have been successfully isolated with various macroscopic and microscopic diversity.
Akumulasi Biomassa Tumbuhan Poaceae di Bawah Cekaman Karbon Monoksida (CO) Puspitasari, Laksmi; Wahyuni, Resti; Pasaribu, Pinta Omas; Saputri, Rinny
Journal of Natural Sciences Vol 6, No 1 (2025): Journal of Natural Sciences Maret
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v6i1.813

Abstract

Air pollution caused by motor vehicle emissions is a significant environmental issue in Indonesia. One of the main pollutants from these emissions is carbon monoxide (CO), which can harm the environment and inhibit plant growth. High carbon monoxide exposure can cause plants to experience stress. This study aims to analyze the biomass accumulation of several plant species from the Poaceae family (Setaria plicata, Rottboellia cochinchinensis, Eleusine indica, and Paspalum conjugatum) under carbon monoxide exposure. The research method used was an experimental study with a Two-Factor Completely Randomized Design (CRD). The factors tested included plant species (four types) and the frequency of carbon monoxide exposure (three levels), with each treatment repeated three times. The results showed that the shoot dry weight was influenced by the plant species and the frequency of carbon monoxide exposure separately, with no interaction between the two factors. Rottboellia cochinchinensis had the highest shoot and root dry weight accumulation compared to the other species, while Paspalum conjugatum had the lowest biomass accumulation. The frequency of carbon monoxide exposure twice a week for two consecutive weeks resulted in the lowest shoot dry weight among the Poaceae plants.
Identifikasi Molekuler dan Potensi Bakteri Endofit dari Tanaman Combretaceae Parapat, Timothy Samuel; Prasetya, Eko
Journal of Natural Sciences Vol 6, No 1 (2025): Journal of Natural Sciences Maret
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v6i1.779

Abstract

Plants from the Combretaceae family are known as medicinal plants, some of which are the genera Combretum and Terminalia. Ketapang (Terminalia catappa) is one of the plants from the genus Combretaceae that can produce beneficial metabolite compounds. In addition to being useful in increasing plant growth, endophytic bacteria have the ability to produce the same metabolites as their host plants. This study aims to identify endophytic bacterial species found in plants of the Combretaceae family and to examine their potential. The methods used in this study are identification of the characteristics of endophytic bacterial cells using a light microscope, molecular identification through the BLAST program, and literature studies. The results of this study are 9 species of endophytic bacteria associated with plants from the Combretaceae family. They are Lysinibacillus fusiformis, Citrobacter freundii, Staphylococcus epidermidis, Enterobacter cloacae, Pseudomonas fulva, Bacillus subtilis, Staphylococcus hominis, Paenibacillus motobuensis, Cronobacter sakazakii, Cytobacillus firmus, and Methylobacterium radiotolerants.

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