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Makara Journal of Science
Published by Universitas Indonesia
ISSN : 23391995     EISSN : 23560851     DOI : https://doi.org/10.7454/mss
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Makara Journal of Science publishes original research or theoretical papers, notes, and minireviews on new knowledge and research or research applications on current issues in basic sciences, namely: Material Sciences (including: physics, biology, and chemistry); Biochemistry, Genetics, and Molecular Biology (including: microbiology, physiology, ecology, taxonomy and evolution); and Biotechnology.
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Articles 880 Documents
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Comparison of Immobilized Metal Affinity Chromatography Ni-NTA and Co-TALON for the Purification of Recombinant Human Erythropoietin Rubiyana, Yana; Santoso, Adi; Batubara, Irmanida
Makara Journal of Science Vol. 19, No. 4
Publisher : UI Scholars Hub

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Abstract

The purification of recombinant proteins is an important stage in biopharmaceutical research. A commonly used technique is immobilized metal affinity chromatography (IMAC). One of the main advantages of this type of chromatography is that the column can easily be regenerated for subsequent purification work. The mechanism of IMAC is based on bonding between metal ions immobilized on a matrix with a specific amino acid. Because of the strong interactions of the electron donor group on the imidazole ring, histidine is often used in the IMAC purification system. Two types of commercial IMAC resin use a nitrilotriacetic acid (NTA) matrix: a nickel-based (Ni-NTA) and cobalt-based (Co-NTA), better known as TALON. This study was aim to investigate the effect of the metal ions Ni2+ and Co2+ to purify recombinant human erythropoietin (rhEPO) expressed in yeast system Pichia pastoris. The results indicated that both Ni-NTA and Co-TALON gave almost the same level of protein purity; however, Ni-NTA has a higher binding affinity than Co-TALON might be due to the higher stability complex of Ni+. The average amount of protein bound by Ni-NTA and Co-TALON was 183.5 and 38.7 µg/mL, respectively.
Co-digestion of Sorghum Stalk and Sludge for Biogas Production Romli, Muhammad; Anggraini, Aulia; Purwoko,; Suprihatin,
Makara Journal of Science Vol. 19, No. 4
Publisher : UI Scholars Hub

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Abstract

The recycling of residual agricultural biomass using anaerobic digestion allows for the recovery of biomass carbon and nutrients as sources of energy and fertilizer. The obstacles that are encountered in this process include the lignocellulosic structure of biomass tissue and its high carbon-to-nitrogen (C:N) ratio. This study evaluates the co-digestion system of pretreated sorghum stalks and wastewater sludge. The stalks were pretreated by partial bio-oxidation to improve their bacterial accessibility. The digesters were fed a mixture of stalk and sludge at ratios of 100:0, 80:20, 60:40, and 40:60 (total solids [TS] basis). The digesters were run in batches at 35-36 °C, with an initial TS of 15%. The digesters’ performance was evaluated in terms of biogas production rate and yield. The digesters that were run with feed ratios of 80:20 and 60:40 showed shorter lag phase, higher biogas generation rates, and higher biogas yields compared to those run with feed ratios of 100:0 and 40:60. The highest specific biogas production (of 122 L/kg TS) was achieved by the digesters run at ratios of 80:20 and 60:40. The digesters run only with stalks (ratio 100:0) resulted in specific gas production of 67 L/kg TS, whereas those fed on a feed ratio of 40:60 generated only 13 L/kg TS. We conclude that the co-digestion of sorghum stalks and wastewater sludge at a proper ratio improves biogas production.
Population Status and Habitat Preferences of Critically Endangered Dipterocarpus littoralis in West Nusakambangan, Indonesia Robiansyah, Iyan; Davy, Anthony John
Makara Journal of Science Vol. 19, No. 4
Publisher : UI Scholars Hub

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Abstract

The conservation of the endemic tree species Dipterocarpus littoralis (Bl.) Kurz. is hampered by the paucity of information on its population biology and ecology. Consequently, a targeted survey was carried out in the West Nusakambangan Nature Reserve to assess its population size and structure as well as habitat preferences. In total, 676 individuals of D. littoralis were located at 52 locations, with an extent of occurrence of 3.66 km2 and an area of occupancy of 1.71 km2. The population had an inverse-J-shaped distribution of diameter at breast height (DBH), with 63% of individuals in the 0-5 cm class and another 21% in the 5-10 cm class; only 11 (1.6%) mature individuals (DBH≥30) were found. D. littoralis was associated with steep, low, southwest-facing sites and sites that had high litter cover and thickness. Illegal logging and fuel-wood chopping were the main threats to D. littoralis and its habitat. In addition, an invasive shrub, Langkap (Arenga obtusifolia, Arecaceae), was a potential competitor with the seedlings throughout the reserve. In view of its endemism, narrow range and localized distribution, small population, environmental preferences, and the severe threats from anthropogenic activities and invasive species, D. littoralis appears to more than justify its conservation status of Critically Endangered.
Microfungi on Leaves of Licuala bidentata (Arecaceae) from Sarawak, Malaysia Lateef, Adebola; Muid, Sepiah; Bolhassan, Mohamad Hasnul
Makara Journal of Science Vol. 19, No. 4
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Abstract

A microfungal survey was carried out on the living leaves and litters of Licuala bidentata in Kubah National Park in Sarawak, Malaysia. A total of 400 leaf segments (200 segments for each leaf type) were plated on two isolation media (water agar and malt extract agar) for endophytic and saprophytic fungal isolation. Forty-three microfungal species were obtained from both leaf types, 31 species identified from living leaves and 18 species from litters. Only six species were common to both leaf types, with 25 and 12 species exclusively identified from living leaves and litters, respectively. New records of fungi from this host plant and for the genus Licuala include Isthmotricladia laeensis, Chloridium sp., Mucor sp., Oidiodendron sp., Kinochaeta sp., Cryptophiale sp., Chrysosporium merdarium and Circinotrichum fertile. This study constitutes the first report on microfungal community on L. bidentata. Implications of this new report in comparison with the microfungal species on other plant species in the genus Licuala are discussed.
In Vitro Formation of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in Calf Thymus DNA upon Treatment of 2'-deoxyguanosine with Propyl Gallate and 2,6-di-tert-butyl-p-benzoquinone Budiawan,; Widiastuti, Dwi Retno
Makara Journal of Science Vol. 19, No. 4
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Abstract

Oxidative DNA damage caused by propyl gallate (PG) and 2,6-di-tert-butyl-p-benzoquinone (BHT-quinone, a metabolite of butylated hydroxytoluene (BHT)) was analyzed from the 8-hydroxy-2¢-deoxyguanosine (8-OHdG) formation in calf thymus DNA and DNA base, 2¢-deoxyguanosine (dG). PG in the presence of CuCl2 increased the 8-OHdG formation in calf thymus DNA by around 9.17 times as compared to the control (untreated DNA). In the presence of CuCl2 at 1.28×10-5 M, the 8-OHdG per dG ratio resulting from the reaction of dG with PG at various concentrations (20–150 ppm) ranged from 75.50 to 312.06 8-OHdG per 105 dG. The 8-OHdG formation increased when the PG concentration was increased from 20 ppm to 80 ppm, and then, it began to plateau around 80 ppm. On the other hand, BHT-quinone increased the formation of 8-OHdG in the presence of CuCl2 by 0.05 times as compared to the control (untreated DNA). LC-MS/MS analysis was used to identify the molecular structure of 8-OHdG, which had a base peak (M+. + 1) at m/z = 284 and two main fragments at m/z = 167.9 and m/z = 139.9.
A Simple and Safe Spectrophotometric Method for Iodide Determination Sulistyarti, Hermin; Atikah,; Fardiyah, Qonitah; Febriyanti, Sita; Asdauna,
Makara Journal of Science Vol. 19, No. 2
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Abstract

In order to practice green chemistry, a simple and safe spectrophotometric method for iodide determination has been successfully developed based on the formation of a blue starch-iodine complex. Iodide was oxidized to form iodine prior to the addition of a starch solution, and the blue starch-iodine complex was directly detected spectrophoto-metrically at a wavelength of 615 nm. The chemical parameters, such as type, reaction time, as well as concentration of oxidizing agents and solution pH were optimized with respect to sensitivity and analysis time. The method showed optimum results under iodate oxidant with a mol ratio of IO3-:I- =1:3, reaction time of 5 minutes, and solution pH of 5. Under these optimum conditions, the method showed linierity measurements from 5-40 mg/L iodide with a correlation (R2) of 0.9889. This technique offers a simple, safe, accurate, and relatively fast method for iodide determination, which is prospective for monitoring iodide samples.
Xylooligosaccharide Production from Tobacco Stalk Xylan using Xylanase Streptomyces sp. BO 3.2 Kholis, Muhammad Nur; Yopi,; Meryandini, Anja
Makara Journal of Science Vol. 19, No. 2
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Abstract

Tobacco stalk (TS), which is one type of lignocellulosic material, has a xylan content of up to 21.9%. Lignocellulose can be used to produce xylooligosaccharides (XOs). XOs are dietary fibers that have prebiotic activity. This study aimed to produce XOs from tobacco stalk xylan using xylanase from Streptomyces sp. BO 3.2. After the TS was delignified, the xylan was extracted using the alkali method. The delignification process, which used 1% natrium hypoclorite (NaOCl), decreased the lignins from 32.93% to 18.15%. Xylan extraction was conducted using 10% natrium hydoroxide (NaOH); this extraction produced xylan of 15.53% (w/w). The xylanase produced by Streptomyces sp. BO 3.2 on a 0.5% TS medium had 5.92 U/mL of activity, with the optimum condition occurring at pH 5.5 and a temperature of 60 °C. The xylanase was stable, at temperature 4 °C and 30 °C for 120 hours. The xylanase Streptomyces sp. BO 3.2 was capable of hydrolyzing 2% TS xylan and 2% beechwood xylan during the first, third, sixth, and twelfth hours of incubation time; it also produced XOs with degrees of polymerization (DP) of 2.18 and 2.15, respectively. A Thin layer chomatography (TLC) analysis indicated that the hydrolysis products were XOs with the absence of xylose, glucose, and arabinose.
Feeding Behavior of Tonkean Macaques (Macaca tonkeana) in Schmutzer Primates Center and Ragunan Zoo, Jakarta Riptianingsih, Fery Dwi; Farajallah, Dyah Perwitasari; Astuti, Dewi Apri
Makara Journal of Science Vol. 19, No. 2
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Abstract

Tonkean macaques are one of seven endemic macaque species on Sulawesi Island. Feeding management in captivity should pay attention to the quality, palatability, and feeding behavior patterns of animals. The goal of this study was to compare the feeding behavior of two social groups of Tonkean macaques at Schmutzer Primates Center (SPC) and Ragunan Zoo (RZ) with different captive management, which was expected to affect feeding behavior. Ad libitum sampling was used to observe daily behavior and hierarchy, while focal animal sampling was used to observe feeding behavior and feed preference. Data were collected from September 2013 until March 2014 with a total of 495 hours of observations. There were significant differences between the daily behavior of two groups of Tonkean macaques. Resting behavior was dominant in RZ group with non-enrichment feed cage, while feeding behavior was more common in the SPC group with an enrichment feed cage. The SPC group spent most of their feeding time in searching for feed, while choosing, carrying and refusing were greater in the RZ group. Both Tonkean macaque groups showed individual dominance in their feeding behavior. Provisioned feed in both locations had different diversity and preference values. The selection of feed required was based on preference values with attention to Tonkean macaques’ feed in nature. Cage construction, such as the SPC cage, was able to reduce abnormal behavior exhibited by individuals.
Optimization of Trypsin-like Protease Production by Lactobacillus plantarum FNCC 0270 using Response Surface Methodology Trismilah,; Nurhasanah, Astutiati; Sumaryono, Wahono; Malik, Amarila; Sadikin, Mohamad
Makara Journal of Science Vol. 19, No. 2
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Abstract

The purpose of this study was to get optimum medium composition and agitation to trypsin-like protease production by Lactobacillus plantarum FNCC 0270. The medium composition and agitation for enzyme production was optimized using Central Composite Design and Response Surface Method with Design Expert software version 7.1.5 Fermentation was carried out in erlenmeyer flasks at initial pH 8, 37 °C, using an incubator shaker at 87.5 rpm. The best results showed an enzyme activity of 1.0 mU/mL, a protein level of 0.557 mg/mL, and desirability value of 0.740. Numerical optimization was performed to approach the ideal state of the fermentation or the desirability value of 1. The medium composition containing of 3.64% baker's yeast, 1.21% glucose, and 0.13% skim milk was used for the fermentation. The enzyme activity of 1.51 mU/mL and protein level of 0.205 mg/mL can be achieved. After numerical optimization, the fermentation process was verified in erlenmeyer flasks with incubator shaking at 77 rpm, initial pH 8, 37 °C, and 15 h fermentation. The verification results showed that the enzyme activity of 1.273 ± 0.227 mU/mL and protein level of 0.248 ± 0.012 mg/mL.
Effect of Mg2+ and Fe2+ Concentrations in Culture Medium on CGF Formation from Microalgae Chlorella Pyrenoidosa Ink and Analysis of Amino Acids by Liquid Chromatography-Mass Spectrometry Kusmiati,; Rahmawati, Witry; Agustini, Ni Wayan Sri
Makara Journal of Science Vol. 19, No. 2
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Abstract

Chlorella pyrenoidosa (C. pyrenoidosa) contains Chlorella Growth Factor (CGF), which consists of proteins and polysaccharides. CGF is located inside the nucleus of cells and is beneficial to humans as a food supplement, an immunity booster, and an antioxidant. CGF formation of C. pyrenoidosa is influenced by medium composition. C. pyrenoidosa INK was cultured in a modified basal medium (MBM) with various concentrations of Mg2+ (0.5, 1.0, and 1.5 g/L) and Fe2+ (3.5×10-4 and 5.0×10-4 g/L). The experiments were performed and analyzed under a completely randomized design using a 2-L bottle with three replications. The results showed that MBM with 1.0 g/L of Mg2+ and 3.5×10-4 g/L of Fe2+ yielded the optimal growth curve for C. pyrenoidosa. Analysis of protein content was carried out using the Lowry method with a spectrophotometer at λ=750 nm, and the obtained results were 0.0974 mg/mL (extract) and 6.4097 mg/ml (supernatant). Furthermore, analysis of glucose content was carried out using the phenol sulfate method (λ = 490 nm), and the obtained results were 49.331 ppm (extract) and 1566.911 ppm (supernatant). Analysis of amino acids in CGF using liquid chromatography-mass spectrometry (LC-MS) indicated the presence of tyrosine, proline, glutamate, alanine, valine, tryptopan, phenylalanine, methionine, and leucine-isoleucine.

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