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Jurnal Sain Veteriner

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Published by Universitas Gadjah Mada

ISSN : 012660421 EISSN : 24073733 DOI : -

Core Subject : Health,

Veterinary

Arjuna Subject : -

Articles 17 Documents

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Search results for , issue "Vol 35, No 2 (2017): Desember" : 17 Documents clear

Studi Distribusi Glukosa Transporter 4 pada Otot Skelet Ayam Kedu Cemani dengan Metode Imunohistokimia Avidin-Biotin-Peroxidase Complex Teguh Budipitojo; Ariana Ariana; Tri Wahyu Pangestiningsih; Hery Wijayanto; Dwi Liliek Kusindarta; Dewi Kania Musana
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Glucose Transporter 4 (GLUT 4) merupakan protein transporter glukosa yang diatur oleh insulin, ditemukan terutama di jaringan adiposa dan otot lurik (baik otot skelet maupun otot jantung). Ayam kedu cemani merupakan ayam endemik Indonesia yang terdapat di wilayah Kedu, Temanggung, Jawa Tengah. Penelitian ini diperlukan untuk melengkapi dokumentasi data anatomi mikroskopik kekayaan hayati asli Indonesia. Penelitian ini bertujuan untuk mengklarifikasi distribusi GLUT 4 dalam serabut otot skelet ayam kedu dengan metode imunohistokimia avidin-biotin-peroxidase complex (ABC). Penelitian dilakukan dengan menggunakan jaringan otot yang meliputi otot pectorales mayor, biceps brachii, dan biceps femoris dari 5 ekor ayam kedu cemani. Hasil penelitian menunjukkan bahwa immunoreaktivitas GLUT 4 terutama terdeteksi di sarkolema dan komponen miofibril pada otot pectorales mayor, biceps brachii, maupun biceps femoris. Intensitas imunorekatifitasnya meningkat dari intensitas lemah pada otot pectorales mayor, menjadi intensitas sedang pada otot biceps brachii, dan kemudian intensitas kuat pada otot biceps femoris. Hasil penelitian memunculkan dorongan untuk menggali lebih lanjut ciri-ciri khusus ayam kedu cemani lainnya, untuk mendokumentasi data anatomis kekayaan hayati asli Indonesia.

Optimalisasi Pembekuan Sperma Limbah Kauda Epididimis Kambing Lokal dengan Metode Bertahap dan Stabilisasi Naela Wanda Yusria Dalimunthe; M. Rosyid Ridlo; Agung Budiyanto
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Buck slaugthering produce waste such as testicles including epididymis which contain fertile sperm. Utilization of cauda epididymis as the sources of sperm for producing goat frozen sperm was not reported yet. The aims of this study were improving the frozen-thawed sperm using stabilization and multistep methods which recovered from the waste of buck slaughtering as the source of sperma. Cauda epididymis spermatozoa which was washed then diluted using extender 1 (Tris-citrate-antibiotics) and extender 2 (extender 1- glycerol-egg yolk). The extender 2 addition was performed by single or multistep methods then freezed. Modification in the pre freezing proces were performed by comparing the conventional equilibration and stabilization methods. The sperm suspension was incubated in 4°C for 2 hours after filling-sealing into straws on the equilibration group whether the stabilization group was cooled in tube 15 mL. All cooled straws from both groups were placed 4 cm horizontally on liquid nitrogen surface for 10 minutes and then plunged into liquid nitrogen for storage. The evaluation of motility parameters such as pattern of the movement and motility percentation were done followed the standard methodology. The student t-test, correlation and one-way ANOVA were used for data analysis with P<0.05. The results showed that multistep dilution method could increase the motility (25.0 ± 1.8 %) compared with single step (18.3 ± 1.7 %). Pre freezing method with stabilization also resulted higher motility (24.2 ± 2.0 %) than equilibration method (17.5 ± 2.8 %). The pattern of the movement were not different between all methods and its combination. The multistep dilution method and stabilization cooling method as well as its combination seems could increase the quality of frozen-thawed cauda epididymis spermatpzoa of local buck.

Potensi Imunologi Serbuk Umbi Tanaman Sarang Semut (Myrmecodia tuberose) Terhadap Tikus Wistar yang Diinduksi Streptozotocin Imron Rosyadi; Bambang Hariono
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Diabetes melitus adalah penyakit metabolik yang ditandai dengan hiperglikemia akibat penurunan kadar hormon insulin yang diproduksi kelenjar pankreas. Banyak obat alternatif yang digunakan untuk mengatasi maupun mencegah penyakit diabetes melitus, salah satunya adalah umbi tanaman Sarang Semut (Myrmecodia tuberose) yang mengandung senyawa polisakarida tinggi untuk meningkatkan sistem kekebalan tubuh. Penelitian ini bertujuan untuk mengetahui pengaruh serbuk umbi tanaman Sarang Semut (Myrmecodia tuberose.) terhadap kadar dan respon imun tikus Wistar yang diinduksi streptozotocin.Tikus yang digunakan adalah tikus Wistar sebanyak 25 ekor jantan, umur sekitar 2 bulan, dengan berat badan 180-250 gram. Tikus dibagi 5 kelompok secara acak masing-masing 5 ekor. Tikus kelompok I, II dan III dibuat diabetes dengan induksi dosis tunggal streptozotocin intraperitoneal 40 mg/kg bb yang dilarutkan dalam buffer sodium sitrat 0,1 M. Tikus diabetes kelompok I diterapi dengan serbuk umbi tanaman Sarang Semut dosis A (18 mg/200 g bb/tikus/ekor/hari/PO) selama 21 hari dan tikus diabetes kelompok II diterapi dengan serbuk umbi tanaman Sarang Semut dosis B (9 mg/200 g bb tikus/ekor/hari/PO) selama 21 hari. Tikus diabetes kelompok III diberi perlakuan 0,5 mL NaCl fisiologis/200 g bb tikus/ekor/hari/PO selama 21 hari sebagai kontrol positif diabetes. Kelompok IV diberi serbuk umbi tanaman Sarang Semut dosis A (18 mg/200 g bb tikus/ekor/hari/PO) selama 21 hari untuk mengetahui efek dari Sarang Semut itu sendiri. Kelompok V diberi perlakuan 0,5 mL NaCl fisiologis/200 g bb tikus/ekor/hari/PO) selama 21 hari sebagai kontrol negatif. Pemeriksaan kadar glukosa darah dilakukan pada hari ke-0, 7, 14 dan 21 terhadap setiap kelompok tikus. Di akhir penelitian, dilakukan uji respon imunologik terhadap fungsi leukosit yaitu uji lazy leucocyte syndrome.Hasil penelitian menunjukkan bahwa serbuk umbi Sarang Semut mampu menurunkan kadar glukosa darah, memperbaiki fungsi imunologik leukosit. Pemberian serbuk Sarang Semut dosis A (18 mg/200 g bb tikus/ekor/hari/PO) memperlihatkan hasil yang lebih baik dibandingkan dosis B (9 mg/200 g bb tikus/ekor/hari/PO). Disimpulkan bahwa serbuk umbi Sarang Semut memiliki potensi sebagai antidiabetes melitus dan mampu meningkatkan respon imunologik.

Infeksi Virus Peste de Petits Ruminants (PPR) pada Kambing dan Domba di Indonesia Indrawati Sendow; Raden Mohamad Abdul Adjid
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Peste des petits ruminants (PPR) merupakan salah satu penyakit virus pada kambing dan domba yang ditandai dengan peradangan pada saluran pencernaan dan pernafasan. Penyakit ini masuk dalam “daftar penyakit” yang dibuat oleh OIE. Keberadaan penyakit ini di Indonesia belum pernah dilaporkan sehingga perlu dilakukan surveilan secara serologis pada kambing dan domba untuk mendapatkan informasi awal keberadaan antibodi terhadap virus PPR. Sebanyak 680 sampel serum kambing dan domba yang berasal dari Rumah Potong Hewan (RPH) Jakarta dan pedagang/pengumpul kambing dan domba di Jakarta pada tahun 2014 telah dikoleksi. Hasil pengujian dengan uji kompetitif ELISA yang menggunakan antigen inaktif (recombinant nucleoprotein- NP), menunjukkan bahwa telah terdeteksi antibodi terhadap virus PPR dengan prevalensi 0,2% pada kambing dan 1,7 % pada domba asal RPH Jakarta. . Berdasarkan lokasi asal ternak yang masuk ke RPH Jakarta, prevalensi tertinggi diperoleh dari Indramayu (2,8%), Solo 0.75%. Serum yang positif, beberapa negatif dan dubius tersebut kemudian diuji untuk dikonfirmasi di Australian Animal Health Laboratory (AAHL) menggunakan uji ELISA dan menunjukkan hasil yang konsisten yaitu tetap positif atau negatif. Selanjutnya dilakukan pengambilan sampel serum dari domba dan kambing di beberapa kabupaten Indramayu pada tahun 2016. Hasil menunjukkan bahwa seluruh sampel sebanyak 326 sera telah diuji dengan uji ELISA hasilnya negatif. Hasil penelitian ini memperlihatkan bahwa infeksi PPR masih sangat rendah, namun hasil ini dapat menunjukkan indikasi awal adanya infeksi PPR di Indonesia. Oleh karena itu, kewaspadaan terhadap meningkatnya kejadian infeksi PPR di Indonesia perlu mendapat perhatian.

Variasi Morfologi dan Deteksi Leucocytozoon caulleryi dengan Metode PCR pada Ayam Ras di Wilayah Endemis Indonesia Endang Suprihati; Wiwik Misaco Yuniarti
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The purpose of this study was to analyze the variation in morphology and detection Leucocytozoon caulleryi by PCR in mtDNA genes cty b that attacking bred chickens in endemic areas of Indonesia. This study was divided into three stages, data collection of Leucocytozoonosis cases in endemic areas of Indonesia; identification of parasites Leucocytozoon caulleryi morphologically through microscopic examination; and the identification of Leucocytozoon caulleryi cyt b genes that attack bred chickens in endemic areas by PCR. The results showed that there are variations in morphology of Leucocytozoon caulleryi that attacking chicken in endemic areas of Indonesia. Gamete morphometry of L. caulleryi had an average length and width 18.233 ± 4.672 dan 12.934 ± 3.349 µm. Nested PCR clearly showed positive reaction of Leucocytozoon infections by amplicons in 600 bp and 03 bp length.

Determination of Cattle and Buffalo Skin Crackers Using Polymerase Chain Reaction Restriction Fragment Length Polymorphism Rulli Riana Dewi; Yuny Erwanto; Nanung Agus Fitriyanto
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The aim of this study was to determine of cattle and buffalo species based on cytochrome b gene using PCR-RFLP. Cattle and buffalo hides were obtained from a slaughterhouse in Yogyakarta and Kudus Regency. To confirm the effectiveness and specificity of this fragment, there are seven of DNA mixture samples in various levels. Isolate DNA samples were amplified using universal primer of cytochrome b gene, then PCR amplicon was digested by RsaI restriction enzyme.. The result showed that mitochondrial cytochrome b gene successfully amplified fragments of 359 bp. RsaI restriction enzyme was able to cleave buffalo cytochrome b gene into two fragment (326 and 23 bp), while the cytochrome b gene of the skin cattle DNA was uncleaved. . In conclusion, this study indicated that mixture DNA of cattle and buffalo hides could be digested by RsaI restriction enzyme and determination of the buffalo hides in mixture samples could be detected into 10% level. Furthermore, RsaI enzyme could be used to specific identification buffalo species. PCR-RFLP technology has a potential and reliable method to identify of the existence of r buffalo hides in the mixture with other hides.

Respon Imun Mencit terhadap Protein 24 dan 71 kDa Toxocara vitulorum dalam Membentuk Antibodi dan Protektifitasnya terhadap Infeksi Buatan Candra Dwi Atma; Kusnoto Kusnoto; Eduardus Bimo Aksono HP.
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

This study aimed to get 24 and 71 kDa protein of T. vitulorum that have a high antigenicity and imunogenicity on ELISA and to get the protein which able to protect mice against artificial infection of L2 T. vitulorum. This study using mice Balb/c aged 6 to 8 weeks. Proteins isolated were 24 and 71 kDa. Proteins 24, 71 kDa and intestinal homogenates immunized in mice with the addition of adjuvant (1: 1) for 3 times with period of 2 weeks. Two weeks after the last booster, serum drawn from mice tested by Indirect ELISA to determine the value of optical density (OD). The next stage, mice were infected L2 with a dose of 10-17 larvae / g of body weight. The results showed the average OD value by ANOVA Factorial antigen P24 was not significantly different with antigen P71 T. vitulorum. Antigen 24 kDa and 71 kDa with different immunization, both were showed P0 significantly different with P1, P2 and P3. Based on percentage of L2 in the somatic tissue of mice, P0 were showed 79.1% of total number of L2 early infection, whereas the treatment of P1 were showed 0.04%, P2 and P3 showed as much as 0.02% and 0.04%. 24 and 71 kDa protein of T. vitulorum that have a high antigenicity and imunogenicity.

Efek Ekstrak Air Biji Pepaya (Carica papaya L.) terhadap Fertilitas Mencit (Mus musculus L.) Betina Muhammad Feraldi Firdaus; Agung Janika Sitasiwi; Siti Muflichatun Mardiati
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Papaya seeds were known as medicinal plant which have anti-fertility potencies. So it could be used as a herbal contraceptive. The purpose of this study was to test the anti-fertility effect of Papaya seed water extract on mice (Mus musculus L.) fertility which proved by pregnancy rate, litters number and sex ratio of litters. This study was designed by Completely Randomized Design, using 15 female Swiss Webster mice that were divided into 5 treatment groups with triplicates. The K(-) group were treated by distilled water; K(+) group were treated by contraceptive pills; P1, P2, P3 groups that were treated by papaya seeds extract with 1.4, 3.5 and 7 mg/animal/day respectively. Each treatment was given orally with 0.5 ml in volume for 21 days. At the end of treatment, mice was mated. The observed parameters were pregnancies occurence, litters number and sex ratio of the litters. The litters number data were analyzed by Analysis of Variance. The results showed there was differences in pregnancy time and sex ratio between control and treatment groups, but there was not significantly different in litters number. It could be concluded that the fertility of mice was inihibited by water extract of papaya seeds.

Efektivitas Terapi Multivitamin, Obat Cacing dan Premiks pada Sapi Terdiagnosa Hipofungsi Ovarium di Wilayah Kecamatan Prambanan, Yogyakarta Niken Widarini; Imbang Ru Beda; Agustina Dwi Wijayanti
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Ovarian hypofunction in cattle is reproduction disorder related to massive economic loss. Etiology of this ovarium disfunction are low quality feed, minimum health concern, poor sanitation and pen problem. Eighty-five (85) Peranakan Ongole (PO), Peranakan Limosin (PL) and Peranakan Simental (PS) cows from Prambanan Sub District of Daerah Istimewa Yogyakarta were diagnosed with ovarian hypofunction by rectal palpation and physical examination. The cows were then programmed for ovary condition recovery by administration of vitamine A,D,E (IM), per oral anthelminthic bolus (Klosantel) and premix mixture in feed given daily for 4 weeks. After 4 weeks, the cows were re-examined. Sixty-two (72,9%) cows were recovered from ovary hypofunction, however ovaries of 23 (21,9%) cows were palpated abnormal, which require another administration of A,D,E (IM). There were no significant difference between the type of cows (PO,PL,PS) with recovery percentages after one programme therapy (P>0.05). It can be concluded that single injection of vitamin A,D, E , per oral bolus of Klosantel, and addition of premix into feed daily for 4 weeks were able to reduce 72,9% ovary hypofunction in Peranakan Ongole, Peranakan Limosin and Peranakan Simental cows at Prambanan sub district, Daerah Istimewa Yogyakarta.

Karakteristik Fisik dan Kimia Telur Burung Mamoa (Eulipoa Wallacei) di Pantai Uwo Uwo Kecamatan Galela Kabupaten Halmahera Utara Yusri Sapsuha; Nur Sjafani; Nurjana Albaar; Hasriani Ishak
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The aim of this research was to determine physicochemical characteristics of Mamoa egg. The eggs were collected about 100 eggs from Uwo uwo beach. Physical quality was observe in Animal Husbandry laboratory, Faculty of Agricultural, Khairun University. The physical characteristics observed were measuring include weight, shape, colour of eggshell, length, diameter, egg index, albumen, yolk, weight of eggshell, haugh unit, and thickness of eggshell. The chemical characteristics consist of moisture content, protein, fat content, fiber content, ash, carbohydrate, cholesterol, and antioxidant. Data were analyzed by quantitative descriptive analysis. The result showed that physical quality of egg were weight = 97.28 g, length = 7.12 cm, diameter = 5.45 cm, index of egg = 71.89%, Percentage of yolk and albumen were 69.12% and 23.24%, haugh unit score = 68.78 and shape of egg was ovale. The chemical characteristic of egg chemical quality were protein content = 15.47%, fat content = 8.31%, carbohydrate = 25.52 %, cholesterol = 387.54 mg/100gr, and antioxidant = 49.08% dpph.

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