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INDONESIA
Jurnal Sain Veteriner
Published by Universitas Gadjah Mada
ISSN : 012660421     EISSN : 24073733     DOI : -
Core Subject : Health,
Veterinary
Arjuna Subject : -
Articles 824 Documents
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Undernutrition dan Anestrus Pada Kambing Bligon Betina Umur 2-3 Tahun: Sebuah Studi Kasus Kelviano Muqit; Irkham Widiyono; Yanuartono Yanuartono; Sarmin Sarmin; Tridjoko Wisnu Murti
Jurnal Sain Veteriner Vol 39, No 1 (2021): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.56917

Abstract

Kajian kasus ini ditujukan untuk mengungkap fenomena klinis dan reproduktif yang dialami 5 ekor kambing Bligon betina umur 2-3 tahun yang dipelihara oleh petani di Yogyakarta. Menurut informasi dari pemilik, kambing mengalami kekurusan dan tidak pernah menunjukkan gejala berahi. Hewan sudah diobati dengan ivermectin secara berkala. Pada hewan tersebut selanjutnya dilakukan kajian manajemen pemeliharaan serta observasi dan pemeriksan klinis (pemeriksaan fisik, uii berahi, pemeriksaan sitologi vagina terhadap organ reproduksi)dalam kurun waktu sekitar 60 hari (akhir Juli- awal September 2019). Pada akhir periode observasi hewan diberi perlakuan gertak berahi dengan pemberian injeksi PGF2-alfa dua kali dengan selang 11 hari dan pemeriksaan USG. Hasil pemeriksaan klini hewan tidak bunting, tidak ditemukan adanya ekto dan endoparasit, tidak ditemukan adanya perubahan fisik, dan tanda-tanda penyakit infeksi. Selama masa pengamatan hewan mendapat pakan berupa jerami kangkung pada level sekitar 2% bobot badan, pertambahan bobot badan harian yang negatif atau rendah, BCS buruk (1-1,5 dalam skala 1-5), tidak ditemukan berahi, gambaran sitologi bagina didominasi sel parabasal dan transisional, respon terhadap pemberian preparat PGF2-alfa tidak menunjukkan adanya perubahan fisik alat kelamin, perilaku berahi, dan gambaran sitologi apus vagina. Hasil pemeriksaan USG tidak menunjukkan adanya status ovarium yang aktif. Hewan didiagnosa mengalami Undernutrition dan anestrus. Berdasarkan pemeriksaan fisik dan pendukung dapat disimpulkan faktor lingkungan berupa asupan pakan yang rendah dan kondisi tubuh yang buruk berpotensi mengakibatkan gangguan reproduksi pada kambing Bligon betina di masa usia produktif.
Mutant Prevention Concentration Siprofloksasin Terhadap Escherichia coli Patogen dari Kloaka Broiler Secara In Vitro Maria Fatima Palupi; Eli Nugraha; Meutia Hayati; Neneng Atikah
Jurnal Sain Veteriner Vol 39, No 1 (2021): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.57040

Abstract

Mutant prevention concentration (MPC) is an in vitro test used to determine the lowest drug concentration needed to inhibit the growth of a single-step-mutant bacterial subpopulation. The purpose of this study was to determine the MPC value of ciprofloxacin against pathogenic Escherichia coli to obtained the range of mutant selection windows (MSW) of ciprofloxacin. Ciprofloxacin is a quinolone group that is included in the Highest Priority Critically Important Antimicrobials for Human Medicine but is also used for the treatment of bacterial infections in production animals. Twenty-four of pathogenic E. coli isolates sensitive to ciprofloxacin were tested to obtain MPC values and minimum inhibitory concentration (MIC) values. Test the MPC and MIC values to get the MSW range is done by the method of agar dilution. Mueller-Hinton agar containing standard ciprofloxacin was inoculated with 1010 cfu E. coli for the MPC test and 104 for the MIC test. Based on the MPC test results, the MPC value of ciprofloxacin was 4-64 μg / mL (22.96 ± 19.07 μg / mL) and there was one isolate which had an MPC> 256 μg / mL. These results give a wide range of MSW with a lower limit of the MIC value of 0.25 - 2 µg / mL (0.55 ± 0.37 µg / mL) to the upper limit of the MPC value of 4-64 µg / mL (22.96 ± 19.07 μg / mL). Based on the results of this MPC assessment it can be concluded that the dose of ciprofloxacin in production animals has a wide range of MSW that is allow for single-step mutants.
Hematology Profile and Liver Histopathology in Escherichia coli Infected Layers Treated with Combination of Phyllanthus ( Phyllanthus niruri L. ) and Turmeric ( Curcuma domestica ) Sri Hartati; Tri Untari; Bambang Sutrisno; Ida Fitriana
Jurnal Sain Veteriner Vol 39, No 1 (2021): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.58423

Abstract

Colibacillosis a disease that can cause considerable economic loss, remains an important health problem. Phyllanthus (Phyllanthus niruri L) and turmeric (Curcuma domestica) are herbs that can be used as immunomodulators. This study was aimed to determine the level of safety of the combination of phyllanthus and turmeric on hematology profile and liver histopathology of layers with colibacillosis. The layers were assigned to the following of 5 groups: a) colibacillosis group without treatment, b) colibacillosis group with 500 mg/kg BW of phyllanthus, c) colibacillosis group with 300 mg/kg BW of turmeric, d) colibacillosis group with phyllanthus and turmeric combination (1:1), e) colibacillosis group with combination of phyllanthus and turmeric (1:2) . After 21 days of treatment, blood and liver sample were collected. The hematological profile (hemoglobin, hematocrit, erythrocyte and leukocyte counts) and liver histology were examined. The result were analyzed statistically by one-way ANOVA. The group that received phyllanthus had higher levels of hemoglobine, haematocrit and erythrocytes than the control group. However, no significant differences were found for the overall groups. Treatment with the combination of turmeric and phyllanthus for 21 days did not cause changes in the hematological profiles or liver histology, and therefor this herbal combination can be used as an alternative therapy for colibacillosis in layers.
Frekuensi Pulsus dan Nafas Sapi Peranakan Ongole Pasca Beranak yang Diinfusi Povidone Iodine 1 % Jumaryoto Jumaryoto; Agung Budiyanto; Soedarmanto Indarjulianto
Jurnal Sain Veteriner Vol 38, No 3 (2020): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.58509

Abstract

Penelitian ini bertujuan mengetahui frekuensi pulsus dan nafas saat recovery uterus sapi potong pasca beranak yang diinfusi Povidon iodine 1%. Penelitian ini menggunakan 10 ekor sapi Peranakan Ongole (PO) yang beranak normal, sehat, umur 4-9 tahun, skor kondisi tubuh 2,5-3,5. Sapi dikelompokkan menjadi 2 yaitu 1). kontrol 2). infusi Povidon iodine 1%. Semua sapi diperiksa secara fisik meliputi kondisi kesehatan umum, penghitungan frekuensi pulsus dan nafas pada hari ke-3 (minggu ke-1), hari ke 38 dan hari ke 68 pasca beranak. Sapi kelompok 2 diberi infusi Povidon iodine 1 % sebanyak 250 ml per-ekor pada hari ke-3. Hasil penelitian menunjukkan semua sapi dalam kondisi sehat dengan frekuensi nafas pada sapi kelompok kontrol hari ke-3, 38 dan 68 berturut-turut adalah 28,80 ± 5,02; 24,00 ± 0,00 dan 25,20 ± 4,38 kali/menit dan pada sapi kelompok perlakuan adalah 27,60 ± 3,29; 22,40 ± 4,34 dan 21,20 ± 5,76 kali/menit. Frekuensi pulsus pada sapi kelompok kontrol hari ke-3, 38 dan 68 berturut-turut adalah 81,60 ± 8,05; 67,20 ± 14,94 dan 62,00 ± 4,90 kali/menit dan kelompok perlakuan adalah 74,40 ± 8,05; 63,20 ± 9,01 dan 72,80 ± 9,86 kali/menit. Uji t-test didapatkan bahwa tidak ada perbedaan yang singifikan (p> 0,05) antara sapi kontrol dan perlakukan. Berdasarkan hasil penelitian ini dapat disimpulkan bahwa sapi PO sehat 3-68 hari pasca beranak mempunyai frekuensi nafas 21,20 ± 5,76 - 28,80 ± 5,02 kali/menit dan frekuensi pulsus 62,00 ± 4,90 - 81,60 ± 8,05 kali/menit.
Pemilihan Antibiotika pada Anjing Diare yang Terinfeksi Escherichia coli Soedarmanto Indarjulianto; Sitarina Widyarini; Gede Bayu Suparta; Alfarisa Nurrurozi; Yanuartono .; Slamet Raharjo; Yeremia Yobelino Sitompul; Ika Tidariani; Anna Ekawati; Mega Cahya Nalasukma
Jurnal Sain Veteriner Vol 39, No 1 (2021): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.60327

Abstract

Diare adalah penyakit yang sering ditemukan pada anjing dan Escherichia coli merupakan salah satu yang dianggap sebagai penyebabnya. Tujuan dari penelitian ini adalah menentukan diagnosis diare pada anjing yang disebabkan E. coli dan menentukan pilihan antibiotika sebagai terapi utama. Sebanyak 3 pasien anjing diare digunakan di dalam penelitian ini. Semua anjing diperiksa secara fisik dan E. coli diidentifikasi dari sampel fesesnya. Sampel feses dipupuk pada agar Harlequin™ E. coli/Coliform Medium dan diinkubasi pada 37 °C selama 24 jam. Koloni yang tumbuh diamati, dicat Gram dan diperiksa di bawah mikroskop. Bakteri selanjutnya diuji sensitivitasnya terhadap amoxicillin, chloramphenicol, doxycycline dan enrofloxacin menggunakan metode disk difusi. Zona pertumbuhan bakteri diukur dan dibandingkan dengan standar. Hasil penelitian menunjukkan bahwa E. coli ditemukan pada semua (3) sampel feses anjing diare. Semua bakteri tersebut sensitif terhadap chloramphenicol dan 1 isolat sensitif terhadap amoxicillin, tetapi isolat lainnya berifat intermedier sampai resisten terhadap amoxicillin, doxycylin dan enrofloksasin. Kesimpulan penelitian ini adalah E. coli dapat berperan sebagai penyebab diare  pada anjing dan chloramphenicol adalah antibiotika pilihan utama untuk terapi.
Kajian Metode Aplikasi Sinkronisasi Birahi menggunakan PGF2α pada kambing terhadap kualitas estrus, konsentrasi progesterone dalam darah dan tingkat kebuntingannya Agung Budiyanto; Faradina Kusuma Savitri; Yuda Heru Fibrianto
Jurnal Sain Veteriner Vol 38, No 3 (2020): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.60468

Abstract

          A study of the application method of estrus synchronization with prostaglandin 2 alpha (PGF2α) preparations in local goats in Yogyakarta to determine the effectiveness of estrus synchronization based on the quality of estrus, the concentration of the hormone progesterone in the blood and the level of pregnancy have been done. The low pregnancy rate in goats is caused by a short time of heat and mating behavior that is not clear, resulting in low reproductive performance. The application of lust synchronization technology is expected to solve this problem. Prostaglandin F2α (PGF2α) is commonly applied in Indonesia intramuscularly (IM), while other methods have no known scientific data on the effectiveness of this method. This study used two methods, namely intramuscular (IM) as a control and intravaginal sponge (IS) method. The IM method is a standard method that is commonly used in synchronizing goat estrus, while the IS method with a smaller dose has never been studied scientifically and in detail. This study was to determine the effective dose required in the IS administration of PGF2α hormone in influencing the quality of estrus, progesterone hormone profile, and pregnancy and compared with IM application as a control. This study used 24 goats that had given birth, were not pregnant, aged 3-5 years, had an average weight of 40 kg and were healthy, divided into 3 treatment groups. Group 1 IM PGF2α hormone (8.25 mg / head), group 2 IS PGF2α hormone (5.5 mg / head) and group 3 IS PGF2α hormone (2.75 mg / head). Estrus detection was carried out by visual observation, mating using a trained male, testing progesterone levels with an ELISA kit and pregnancy examination with ultrasound. The results were analyzed using ANOVA and T-Test. The results showed that there was no difference in estrous quality (P> 0.05) at the peak of estrus in each group. Progesterone levels in the IS group with a dose of PGF 5.5 and 2.75 mg / head indicated that the dose of 5.5 mg / head was lower than that in the 2.75 mg / head group. The pregnancy rate of the IM group was not significantly different from the IS group. The conclusion of this research is that synchronization of estrus using PGF2α by IS would to be effectively produce the quality of lust, the level of progesterone in the blood and pregnancy which are not different from the IM administration. The IS method can be applied to synchronization of goats in Indonesia at a lower cost, but the level of efficiency and effectiveness is not different from the IM method which uses a higher dose.
Tantangan dan Kendala Pengendalian African Swine Fever Roza Azizah Primatika; Etih Sudarnika; Bambang Sumiarto; Chaerul Basri
Jurnal Sain Veteriner Vol 39, No 1 (2021): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.61084

Abstract

African Swine Fever (ASF) is a highly contagious hemorrhagic viral disease that attacks pigs and wild pigs causing economic losses for farms small and large scale. ASF outbreaks that occurred in several regions in the world have caused unrest for the livestock sector. The rapid spread of the ASF virus has resulted in very high pig mortality. ASF virus transmission can occur through direct or indirect contact. Urine and faecal excretion of pigs is an important route of ASF transmission. The ASF virus has three transmission cycles, namely the silvatic, domestic and wild boar cycles. Outbreaks that occur in several countries encourage the strategy of controlling and overcoming the disease through surveillance. ASF disease control that has been carried out includes improving farm biosecurity management systems and limiting the movement of animals and animal products before the ASF vaccine is found.
Pengaruh Pemberian GnRH pada Sapi Potong yang Mengalami Kawin Berulang (Repeat Breeding) Surya Agus Prihatno; Sri Gustari; Asmarani Kusumawati; Agung Budiyanto; Erif Maha Nugraha Setyawan; Yosua Kristian Adi
Jurnal Sain Veteriner Vol 39, No 1 (2021): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.62761

Abstract

Repeat breeding is a condition in which cows have normal estrous cycles and have been mated 3 times or more but are not pregnant. The incidence of repeat breeding in beef cattle can be detrimental to breeders in terms of time, that the calving period becomes longer so that the cows cannot give birth to calves once a year. Various ways to deal with repeat breeding have been done, but the results are not optimal. This study was conducted to determine the effect of Gonadotrophin Releasing Hormone (GnRH) in cows with repeat breeding. A total of fifteen beef cattle with repeat breeding were used in this study. The cows were divided into 3 groups of 5 each. Cows that were estrus in the first group (as a control group) were only performed artificial insemination (AI) without GnRH injection. In the second and third groups, after AI, they were injected with GnRH at AI time and 3-4 days after AI with a dose of 5 ml (Fertagyl®, 500 µg gonadorelin) intramuscularly. A pregnancy examination was carried out three months later and the results were recorded. The data obtained were analyzed descriptively and quantitatively using Chi-Square statistical analysis. The results of the analysis showed that there were differences in the pregnancy rate in the first group (control) with the second cow group and the third cow group, which were 20%, 80%, and 60% respectively. Based on statistical analysis, there was a significant difference in pregnancy rate (P <0.05) between the three groups. It was concluded that GnRH administration could increase the pregnancy rate in beef cattle that had repeat breeding.
Evaluasi Pemberian Obat Diminazene Aceturate Secara In Vivo Pada Mencit (Mus musculus) yang Diinfeksi Isolat Trypanosoma evansi Reza Yesica; Bambang Sutrisno; Wisnu Nurcahyo
Jurnal Sain Veteriner Vol 39, No 2 (2021): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.43100

Abstract

Abstract Surra's disease is caused by Trypanosoma evansi parasite has been established as one of the strategic infectious animal diseases. Drug resistance in this case is one of the major challenges in handle and control them. The aim of this study is to evaluate the provision drug resistance diminazene aceturate (Tryponil®) on Trypanosoma evansi isolate from Pemalang and Brebes Central Java province with in vivo test in mice. Total 50 mice, BALB / c strain, male, 2 months, body weight ± 30 gram are obtained from LPPT-UGM, adapted for one week. Mice were divided into 10 groups consist of 5 each. Each mouse was infected with Trypanosoma evansi by intraperitonial route. Treatment was given when mice had reached the level of parasitemia 108 – 109 trypanosoma / mL of blood this was predicted 24 hours post-infection (Eisler et al., 2001). The administration of the drug tryapanosidal was done intraperitonial with doses 1mg/kg, 3mg / kg, 5 mg / kg and 7mg / kg. Observation of parasitemia did every 2 times in one week till 60 days of observation. Parasitemia observation was performed using 3 techniques. The first method was native examination used a microscope, if the negative results would be followed by MHCT (Microhaematocrit centrifugation Technique) and BCT (Buffy Coat Technique) according to OIE (2012). Data obtained from the treatment group were the level of parasitemia, the number of deaths and the number of live mice from each test dose. The results are analysed by standard logit or probit. The results of this study showed the effects of the drug Dimianzene aceturate on both isolates varied. On Brebes Isolate was effective at doses of 7 mg / kg BW (100%) and 5mg / kg BW (80%), whereas in the effective dose Pemalang isolate at 3 mg dose / kg BW (80%), 5 and 7 mg / kg BW (100%). While at the lowest dose of 1 mg / kg obtained a level of effectiveness of 0% in both isolates. It could be concluded that both isolates have different pathogens and indicate resistance subpopulation to diminazene aceturate.Keywords : diminazene aceturate, in vivo, resistance, Trypanosoma evansi. 
Suplemen Calcitriol Dosis Tinggi Mencegah Osteoporosis dan Memicu Urolitiasis pada Tikus Ovariektomi hartiningsih hartiningsih; devita anggraeni
Jurnal Sain Veteriner Vol 39, No 2 (2021): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.47032

Abstract

Calcitriol supplementation in ovariectomized rats for 8 weeks reduce osteoporosis risk and safe for kidney depend a proper dose. The objective of the research was to study the efectivity of high dose calcitriol for osteoporosis prevention and safe for kidney  in ovariectomized Wistar rats. Fifteen female Wistar rats at 8 weeks of age were randomly divided into three  groups of five which were sham operated rats (KN), ovariectomized rats (KOV), and ovariectomized rats+calcitriol supplementation 200ng/day (OVD200). After 8 weeks of treatment, blood samples were taken from plexus orbitalis medialis for estrogen analysis. All rats were then euthanized, left tibia and left kidney were taken for histopathological examination using hematoxylin and eosin stain. Immunohistochemistry using anti TRAP5b monoclonal antibody was also done for left tibia by streptavidin-biotin.  The results showed that estradiol level of KOV was significantly decreased compared with KN (P<0.05), meanwhile, estradiol levels of OVD200 were not significantly different compared with KOV. Histopathologic figure of kidney in KOV was not different compared with KN. Glomerulus was surrounded by Bowman‘s capsule, proximal and distal convoluted tubulus were lined with typical cuboidal epithelium. Immunohistochemistry result of proximal tibial epiphysis in KOV were shown more tartrate resistant alkaline phosphatase 5b (TRAP5b) expression in trabecular bone, which was located in bone marrow space, and trabecular speculum surface as well. Meanwhile, proximal tibia epiphysis in OVD200 had less tartrate resistant alkaline phosphatase 5b (TRAP5b) expression compared with KOV. Histopathologic figure  of proximal tibia epiphysis in group OVD200 was shown more trabecular bone speculum and less adipocyte in the bone marrow compared with KOV. Histopathologic figure of kidney in OVD200 was showed glomerular  hypertrophy and atrophy, as well as protein deposits in proximal convoluted tubules epithelium.  In conclusion, calcitriol supplementation 200ng/day in ovariectomized rats for 8 weeks prevented osteoporosis risk but trigger urolithiasis. Key words :  : Calcitriol, osteoporosis, ovariectomized rats, urolithiasis

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