cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Nurul Hidayah
Contact Email
sccrlaboratory@gmail.com
Phone
+628164251646
Journal Mail Official
dr.agungptr@gmail.com
Editorial Address
Jalan Kol.R.W Sugiarto, Nongkosawit, Gunungpati, Kota Semarang, 50223, Indonesia
Location
Kota semarang,
Jawa tengah
INDONESIA
International Journal of Cell and Biomedical Science
Published by Stem Cell and Cancer Research Indonesia
ISSN : -     EISSN : 28296621     DOI : https://doi.org/10.59278/
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
International Journal of Cell and Biomedical Science, formerly CBS Int. Journal is an open-access, peer-reviewed journal published by Stem Cell and Cancer Research (SCCR), Indonesia. The journal publishes papers describing original findings and reviews articles in all aspects of cell, molecular biology, and biomedical research. Received manuscripts are accepted for publication only after rigorously being reviewed by independent experts in the respective fields determining the originality, validity, and conclusions.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 5 Documents
 1 
Search results for , issue "Vol 1 No 1 (2022)" : 5 Documents clear
Hypoxia Precondition Enhance the Therapeutic Effects of Mesenchymal Stem Cells via regulating TGF-β1 and IL-10 serial expression in Skin Excision Rat Models Kustyah, Azizah Retno; Fatimah, Nandiah; Rizkiyani, Elytia Mutia; Ramadhanti, Olifiarsy Wiet; Istiqomah, Dyah Ayu Fitri; Hidayah, Nurul; Bhirau Wilaksono
International Journal of Cell and Biomedical Science Vol 1 No 1 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i1.9

Abstract

Background: The skin excisional wound healing process involves an intricate-regulated series of cellular responses to reverse the formation of skin tissue integrity. This process requires paracrine communication involving anti-inflammatory cytokines and growth factors, especially interleukin 10 (IL-10) and TGF-β. On the other hand, hypoxic preconditioned mesenchymal stem cells (Hypoxia-MSCs) have been acknowledged to enrich IL-10 and TGF- β secretion contributing to accelerated wound healing compared to normal preconditioned mesenchymal stem cells (Normoxia-MSCs). Objective: This study aimed to compare Hypoxia-MSCs and Normoxia-MSCs in integrating the serial expression of IL-10 and TGF-β associated with improved collagen density in animal models of excision wounds. Methods: Thirty-six male Wistar rats with excision wounds were made as animal models using the 6 mm biopsy method. The rats were randomly divided into four groups consisting of four treatment groups: N-MSCs 1x106, H-MSCs 1x106, Control (PBS treatment), and Sham (untreated or healthy mice). The treatments were administered 2 times intraperitoneally on day 0. Skin tissue was collected on days 12, 18, and 24 post-injections. IL-10 dan TGF-β expressions were examined by qPCR. Results: This study showed that there was a significant increase in IL-10 and TGF-β after Hypoxia-MSCs and Normoxia-MSCs treatment compared to the Control group. Conclusion: Hypoxia-MSCs can improve the serial expression of IL-10 which leads to wound repair of the mouse model of excision wound. These results suggest that a hypoxic environment can enhance the therapeutic effect of MSCs.
Application of Bioinformatics Analysis to Identify Important Pathways and Hub Genes in Breast Cancer Affected by HER-2 Tjipta, Arya; Hermansyah, Dedy; Suzery, Meiny; Cahyono, Bambang; Amalina, Nur Dina
International Journal of Cell and Biomedical Science Vol 1 No 1 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i1.11

Abstract

Human epidermal growth factor receptor 2 (HER-2) is used as a marker for the diagnosis and prognosis of breast cancer. However, the molecular mechanisms involving HER2 in breast cancer require further study. Herein, we used the bioinformatics approaches to identify important pathways and hub genes in breast cancer affected by HER-2. The results showed that HER-2 is highly expressed in ovarian cancer and is closely related to the overall survival and progression-free survival of breast cancer. A total of 3014 downregulated genes and 4121 upregulated genes were identified under Gene Expression Omnibus (GEO) database with the GEO2R tool. Among them, the top 10 hub genes including CCNB1, KIF11, BUB1B, TOP2A, ASPM, MAD2L1, BUB1, RRM2, EGFR, and FN1 demonstrated by connectivity degree in the protein-protein interaction (PPI) network were screened out. In Kaplan–Meier plotter survival analysis, the overexpression of CCNB1, EGFR, MAD2L1, ASPM, and RRM2 were shown to be associated with an unfavourable prognosis in HER-2 positive breast cancer patients. In conclusion, we have identified important signalling pathways involving HER-2 that affect breast cancer. These findings could provide new insights outlining mechanisms involving HER-2 gene expression in breast cancer and provides a rationale for the novel treatment of breast cancer.
Topical Gel of Mesenchymal Stem Cell-Conditioned Medium-induced Serum Injury Accelerates Wound Healing in Skin Excision Tissue Berlian, Mukti Arja; Alif, Iffan; Subchan, Prasetyowati; Handoyo, Frigi Eko; Husain, Sofian Azalia; Husni Ahmad Sidiq; Arlinda, Dyken Dwi; Adityani, Resanti
International Journal of Cell and Biomedical Science Vol 1 No 1 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i1.12

Abstract

Introduction: Umbilical cord-derived mesenchymal stem cells (UC-MSCs) accelerating wound closure by increasing VEGF and PDGF level leading to re-epithelialization, cell infiltration, and angiogenesis. It has been found that MSC-conditioned medium (MSC-CM) can enhance migration of fibroblasts in scratch assays. However, the effect of MSC-CM-induced serum injury (MSC-CM-S) formulated in gel to accelerate wound healing remains unclear. This study aims to evaluate the effect of several doses of topical gel of MSC-CM-S in accelerating wound healing. Methods: The MSCs were cultured medium-supplemented serum injury of wounded rat (8:1) to get MSC-CM-S. The topical gel of MSC-CM-S was made by base gel supplemented with MSC-CM-S. Eighteen Wistar rats were randomly assigned into control (C) and treatment groups (T1, T2). Groups were received serum-free medium gel (C), 25 µl MSC-CM-S in topical gel (T1), 50 µl MSC-CM-S in topical gel (T2), twice daily for 9 days. PDGF and VEGF level and fibroblast density were measured by ELISA and HE staining at day 3 and 6, respectively. Results: This study showed that there was significant increase of VEGF and PDGF level along with a significant increase of fibroblast density at day 3 and 6. The T2 showed optimum enhancement level of VEGF, PDGF and fibroblast density. Conclusion: Topical gel of MSC-CM-S was effective to accelerate wound closure by enhancing PDGF and VEGF level in full-thickness skin defect rats.
Downregulation of IL-6 and TNF-α Expression with Mesenchymal Stem Cells Therapy in Allergic Rhinitis Rats Models Restimulia, Lia; Dewi, Dian Andriani Ratna; Nazar, Mohammad Ariq; Irawan, Risky Chandra Satria; Ghaissani, Shabrina Syifa; Haryono, Erlina
International Journal of Cell and Biomedical Science Vol 1 No 1 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i1.13

Abstract

Rhinitis is an inflammatory that characterized by nasal symptoms due to the condition of the nasal mucosa that trigerred by an interaction between environmental allergens and immunoglobulin (Ig)E. It is driven by host factors, infection, pathogens and various inflammatory pathways such as TNF-α that released in allergic responses from both mast cells and macrophages through IgE-dependent mechanisms. Secretome hypoxia mesenchymal stem cells (SH-MSCs) contain anti-inflammatory soluble molecules were able to improve the conditions of endothelial damage, inflammation and oxidative stress by decreasing levels of IL-6, TNF-α, ROS and increasing eNOS. This study will investigate the effect of SH-MSCs at a dose of 300 µL on TNF- and IL-6 expression in rat model ovalbumin-induced rhinitis in vivo. Thirty male Wistar rats were randomly divided into 3 groups: control, treatment and sham group that administrated through intramuscular injection. The results of this study found that SH-MSCs can downregulate the inflammatory cytokines TNF-α and IL-6 in rat model ovalbumin-induced rhinitis in vivo
X-Ray Scanning Reduce Soluble Active Molecules of Mesenchymal Stem Cells Putra, Agung; Pasongka, Zenitalia; Widyatmoko, Agus; Prasetio, Ardi; Dirja, Bayu Tirta
International Journal of Cell and Biomedical Science Vol 1 No 1 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i1.14

Abstract

Mesenchymal Stem Cells secrete various anti-inflammatory and regenerative SAMs-MSCs that possess immunomodulatory properties and may accelerate wound healing. As a potential agent for therapeutic, SAMs-MSCs should be stable in any condition, including under X-Ray Scanning. The previous study reveal that X-Ray Scanning may induce protein damage. However, the investigation regarding the stability of SAMs-MSCs under X-Ray Scanning is limited, thus this study aimed to investigate the stability of SAMs-MSCs after X-Ray Scanning. Mesenchymal Stem Cell medium was filtrated using the TFF method at 300 and 5 kDa filter cut off and sterilized using 0,1 um syringe. The SAMs-MSCs underwent X-ray Scanning using public Air Port X-Ray twice. The SAMs-MSCs concentration was measured using ELISA. T-test analysis was performed for the statistical analysis with P<0,05. This study revealed that X-ray scanning reduce the concentration of SAMs-MSC. A previous study found that x-ray irradiation may damage protein at 6–18 keV caused by the energy deposited by photoelectrons that are generated by the interaction of X-ray photons and the protein leading to photoelectron-induced damage.

Page 1 of 1 | Total Record : 5

 1 

Filter by Year

2022 2022


Reset
Filter By Issues
All Issue Vol 4 No 11 (2025) Vol 4 No 10 (2025) Vol 3 No 9 (2024) Vol 3 No 8 (2024) Vol 3 No 7 (2024) Vol 2 No 6 (2023) Vol 2 No 5 (2023) Vol 2 No 4 (2023) Vol 1 No 3 (2022) Vol 1 No 2 (2022) Vol 1 No 1 (2022) More Issue