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Life Science and Biotechnology
Published by Universitas Jember
ISSN : -     EISSN : 29884713     DOI : https://doi.org/10.19184/lsb
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Life Science and Biotechnology publishing rigorously peer-reviewed research that seeks to advance our understanding of fundamental processes in biology. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, and the public worldwide. Our journal publishes peer-reviewed research of broad interest in all areas of biology and at the interface of related disciplines (Genetics, Biodiversity, Biometry, Bioinformatics, Evolution, Ecology, Physiology, Biotechnology, Developmental biology, and Biomedical science).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ecology, Evolution, Behavior and Systematics
Articles 25 Documents
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Screening Ethanol-Producing Yeast Origin from Pineapple Honey Ananas comosus (L.) Merr var Quenn Sutoyo, Sutoyo; Siswanto, Siswanto
Life Science and Biotechnology Vol. 1 No. 1 (2023): May
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i1.39560

Abstract

The availability of fossil-based fuels will gradually decrease while the amount required increases. This has prompted research to find alternative energy sources, including bioethanol. Bioethanol, as an alternative energy source, can be used as a transportation fuel better than fossil-based fuels because ethanol has a high octane number, laminar velocity and heat of vaporisation. Meanwhile, the level of carbon and hydrocarbon emissions is low so it can increase combustion efficiency in vehicles. In addition, the use of bioethanol as fuel is environmentally friendly and renewable. Ethanol can be produced through a fermentation process using ethanol-producing yeast. Pineapple honey is known for its high sugar and other nutrient content, so it is supposed that many types of yeast can grow well in it. This study aims to isolation and screening of bioethanol-producing yeast from Pineapple Honey. This study was carried out by yeast isolation method, primary yeast screening, secondary yeast screening, and characterisation of selected yeast isolates. Data on ethanol production by yeast isolates were analysed by ANOVA with α=0.05 using the R studio program. This primary isolation and screening phase found eight yeast isolates capable of fermenting glucose and producing acid and gas in the test medium. Test ethanol production in DPE medium with 18% glucose content on all isolates showed that all isolates could produce ethanol with a range of 4.20-10.35%. Isolates of IK4 showed the highest ethanol production ability (10.35%) (p<0.5%) in significant contrast to the other seven isolates. Based on macroscopic and microscopic morphological characterisations, IKM 4 isolates show similarities to the genus Saccharomyces Keywords: ethanol, pineapple, yeasts
Poiploidy induction of Indonesian Black Rice Oryza sativa L. Var. Cempo Ireng with Bio-catharantine Kurniawan, Ludfi; Laili, Alvina Nur; Angraeni, Devi Silvia; ‘Ain, Salsabila Qurrotu; Wulandari, Dyah Retno; Ulum, Fuad Bahrul
Life Science and Biotechnology Vol. 1 No. 2 (2023): November 2023
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i2.43753

Abstract

High demand on black rice Oryza sativa L. due to their high antioxydant content and better nutrition than the white rice. On the other hand this high price rice still less interest for the farmet to cultivate it. Therefore, improvement of plant characters was carried out through polyploid induction using the natural anti-mitotic compound bio-catharanthine. The purpose of this study was to test the effectiveness of bio-catharanthine in inducing polyploidy in Cemp ireng black rice. This study used a two-factor completely randomized design (CRD) method with three replications. The treatment factor of bio-catharanthine concentration was 1%, 1.5 %, 2 %, 2 %, 2.5 % and 3 % with the soaking time of 48 hours. The germination rate, ploidy level, stomatal size and density, and antioxidant were measuret to observed the characteristic of the mutan. The results showed that the treatments of 3 % bio-catharanthine enhanced the chromosome number of black rice Cempo ireng. Biocatharantine did not affected the germination rate through the treatments. Out mutants shows alternation on the stomatal size and density. The antioxidant of leaf sample did not changed after the treatment. Bio-catharanthine on high concentration migh be possible to be applied on polyploidy induction in black rice
A Preliminary Study of The Javan Leopard Behaviour in Meru Betiri National Park Kholiq, Nur; Sucipto, Adi
Life Science and Biotechnology Vol. 2 No. 1 (2024): May 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i1.47666

Abstract

Meru Betiri National Park (MBNP, 526km2), is one of only three protected areas in east Java that can support a viable Javan leopard population. The Javan leopard has become a priority for management following the extinction of the Javan tiger. Therefore, it is important to know how the Javan Leopard behaves to improve species management. We conducted a field study using camera traps to determine the daily behavior of the Javan leopard in MBNP. A monitoring site was established with 73 stations, with a density of one station per 0.5 x 0.5 km grid cell, providing different but comparable densities and spatial coverage. The results indicate that the Javan leopard displays 9 daily behaviors including walking, observing, standing, cheek rubbing, scraping, urine spraying, claw marking, parenting, vocalizing, and others. Each behavior was observed with a relative frequency of walking (63.25%), observing (14.53%), standing (7.69%), cheek rubbing (5.98%), scraping (4.27%), urine spraying (1.71%), claw marking (0.85%), parenting (0.85%) and vocalizing (0.85%). An analysis of the time spent on each behavior revealed their proportion as follows: walking (382 s, 49.29%), watching (208 s, 26.84%), standing (66 s, 8.52%), cheek rubbing (27 s, 3.48%), scraping (57 s, 7.35%), urine spraying (17 s, 2.19%), vocalizing (9 s, 1.16%), claw marking (4 s, 0.52%) and parenting (5 s, 0.65%).
The matK Mini-barcode as a Potential Molecular Identification Tool for Medicinal Orchids Su'udi, Mukhamad; Setyati, Dwi; Khoirunnisa, Gita Ayu; Afidah, Yusfi; Ulum, Fuad Bahrul
Life Science and Biotechnology Vol. 1 No. 1 (2023): May
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i1.39851

Abstract

matK is one of the universal loci which is commonly used as a barcode in the molecular identification of plants. Up to now, the problem of matK as a single locus is the use of the entire gene region as barcodes. The long amplicon size of matK raises several problems in DNA barcoding. The aim of this study is to uncover a small region of the matK gene as a potential mini-barcode region for alternative sites to get more efficient and effective results. In silico studies performed include the collection of matK sequence of medicinal orchid from GenBank, the amplicon size from matK primer sets that are commonly used in the Orchidaceae family, and PCR analysis. Data analysis was done by comparing the results of PCR amplification from several primer sets: matK 390F-1326R, 743F-R2, 712F-1154R and 712F-1216R. The results show that all primer pairs are able to amplify the DNA template isolated from Dendrobium purpureum. However, the shorter DNA band obtained from matK 743F-R2, 712F-1154R and 712F-1216R primers sets were visualized more thick than the longer amplicon band produced from matK primer 390F-1326R. This indicates that short matK sequence for DNA barcoding (mini-barcode) is more efficient in amplifying DNA templates compared to generally applied barcoding using full length sequences.
in-silico PASS prediction for medicinal potency of antioxidant from Ranunculus japonicus Thunb. Amalini, Yusrina Risky; Afidah, Yusfi
Life Science and Biotechnology Vol. 1 No. 2 (2023): November 2023
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i2.45078

Abstract

Ranunculus japonicus has been traditionally used to treat various ailments, including malaria, jaundice, gastric problems, toothache, and eye inflammation. Recent studies reported that the plant extract effectively treat rheumatoid arthritis due to its secondary metabolites with antioxidant activities, such as flavonoids and phenols. In this study, we analyzed the flavonoids and phenols from R. japonicus using in silico methods. The study determined the flavonoid and phenol contents based on existing literature. The antioxidant potential analysis was conducted using PASS (Prediction of Activity Spectra for Substances). The results indicate the presence of two flavonoid compounds, namely Apigenin 6,8-di-C-|A-L-Arabinopyranoside and 3-Methylquercetin, and three phenol compounds, namely 3-Hydroxytrisol 3-O-glucoside, 4-Hydroxy-3-methoxyacetate, and Hydroxytrisol. Two compounds exhibited high bioactive potential (Pa value > 0.7), two compounds showed medium bioactive potential (Pa value > 0.5), and one compound exhibited low bioactive potential (Pa value < 0.5). The most bioactive compounds were Apigenin 6,8-di-C-|A-L-Arabinopyranoside and 3-Methylquercetin. The compounds with medium bioactivity were 3-Hydroxytrysol 3-O-glucoside and 4-Hydroxy-3-methoxycinnamic. The PASS online analysis revealed the potential of antioxidants for medicinal potencies
Blood Cell Profiles and Metamorphosis of Rice Field Frog (Fejervarya cancrivora) after Heavy Metal Copper (II) Sulfate Exposure Haryanto, Haryanto; Fitri, Fadillya Maulidilla; Nurhayati, Sari; Fadilla, Aghniya Nur Rizka
Life Science and Biotechnology Vol. 2 No. 2 (2024): November 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i2.52293

Abstract

Tadpoles represent a critical stage in frog metamorphosis and are particularly susceptible to environmental stressors. Heavy metals, such as copper, are common industrial pollutants that adversely affect aquatic ecosystems. This study aimed to evaluate the effects of copper (II) sulfate on the blood cell profiles and metamorphic development of rice field frog (Fejervarya cancrivora) tadpoles. Experimental treatments included a positive control (mineral water), a negative control (Propylthiouracil / PTU 0.1 gL-1), and exposure to heavy metal of copper (II) sulfate 0.01 mgL-1 (Cu (II)). Each treatment group comprised nine tadpoles, maintained for 14 days under controlled conditions. After 14 days, the survival rate of tadpoles exposed to Cu (II) was 66.67%. Analysis of variance (ANOVA) revealed that Cu (II) exposure significantly impacted blood cell profiles (p < 0.05). Specifically, Cu (II) exposure led to reduced nuclear size, increased immature erythrocytes, and a higher percentage of abnormal erythrocytes. Although metamorphic growth parameters did not differ significantly among treatments (p > 0.05), Cu (II) exposure accelerated skin pigmentation and induced tail abnormalities, including tumor-like formations. These findings demonstrate that Cu (II) is toxic to the blood cells and induces morphological abnormalities in F. cancrivora tadpoles, highlighting the potential ecological risks of copper contamination in aquatic habitats.
Bioactivity of Endophytic Fungal Extract Isolated from Purun (Eleocharis dulcis (Burm.f.) Trin. ex Hensch) Oktiansyah, Rian; Pratiwi, Mardiyah; Kharisma, Sully Pudja; Fauzan, Ahmad Rizki; Utami, Riza; Amelia, Dea; Noviyanto, Noviyanto; Nasution, Sakinah Salman Ahmad
Life Science and Biotechnology Vol. 2 No. 2 (2024): November 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i2.52579

Abstract

The study investigates the bioactivity of endophytic fungi isolated from Eleocharis dulcis (Burm.f.) Trin. ex Hensch., focusing on their potential as alternative sources of bioactive compounds. E. dulcis, a plant commonly found in wetland ecosystems, has traditionally been used for its medicinal properties. However, the massive extraction of the plant may lead to ecological imbalance, prompting the exploration of endophytic fungi as a sustainable alternative. The objective of this research is to isolate, identify, and evaluate the antimicrobial and antioxidant properties of endophytic fungi from Eleocharis dulcis. Endophytic fungi were isolated aseptically, followed by morphological identification. Antioxidant and antimicrobial assays were conducted to assess the bioactivity of the fungal extracts. Antioxidant activity was evaluated using the DPPH method, while antibacterial activity was assessed using the disc paper diffusion method. The results revealed that several isolates (from leaves: LP1 – LP6, from root: RP1 – RP3) exhibited significant antimicrobial activity against Escherichia coli and Staphylococcus aureus, with Penicillium spp. and Cladosporium spp. showing the highest levels of bioactivity. Additionally, all fungal isolates demonstrated very strong antioxidant activity, with most achieving an IC50 below 20 µg/mL. In conclusion, the findings suggest that endophytic fungi from E. dulcis represent a valuable source of bioactive compounds with potential applications in pharmaceuticals. Further research is required to isolate and characterize the active compounds, as well as to evaluate their efficacy through in vivo studies
Does the chick of domestik chicken (Gallus gallus domesticus) in early development would able to witstand the injection of crude LPS? lelono, Asmoro; Laensugi, Agatha Dei Gratia; Arimurti, Sattya
Life Science and Biotechnology Vol. 2 No. 2 (2024): November 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i2.53503

Abstract

Preventing bacterial infections in poultry farms is a key aspect of effective disease management strategies. Enhancing innate immunity serves as a vital preventive measure in chicken farming. Lipopolysaccharide (LPS), a fundamental component of the bacterial cell wall, has been successfully utilized to boost the innate immune response in susceptible poultry hosts. This study focused on examining the impact of administering crude LPS from Salmonella typhimurium to a domestic chicken (Gallus gallus domesticus) strain during its early developmental stages up to the pre-maturation phase. Immune responses in the chicks were evaluated by observing changes in body temperature and leukocyte differentiation. The chickens were divided into two groups: control (injected with 0.85% NaCl) and treatment (injected with the LPS). The injections were applied in three different ages 7 days, 24 days, and 41 days. The body temperature and leukocyte differential data were collected following the LPS injection. Results showed a significant difference in body temperature and heterophil at the ages of 24 and 41, indicating an immune response characterized by inflammation and elevated heterophil levels. There was a significant increase in basal temperature during early development in line with chicks' development to maintain their homeostatic system. This study concludes that the chicks demonstrated resilience to the presence of LPS by exhibiting heightened responses. The increasing of heterophils concentration provided strong evidence of their immune capability to overcome bacterial infections
In Silico Study of Genus Marchantia using matK loci for DNA Barcoding Farhan, Abdillah Maulana; Ulum, Fuad Bahrul
Life Science and Biotechnology Vol. 2 No. 2 (2024): November 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i2.53527

Abstract

Marchantia is a genus of liverworts that can be found in the Asia-Europe Region. Some of the types that can be found are Marchantia polymorpha subsp. montivagans, Marchantia polymorpha subsp. ruderalis, Marchantia paleacea subsp. diptera. All of these species have different levels of kinship, this is due to differences in their nucleotide structure. The purpose of this study was to identify the intolerance between Marchantia species in genomic studies using the NCBI Database. The method used in this research is the collection of nucleotides on NCBI and then construction with Mega 11 software. The marker used in this study is Maturase K (matK), this is because the marker is very optimal in the identification of a molecular-based species. The results of this study are Marchantia paleacea subsp. diptera matK (MP 58 and MP 54) has the highest level of kinship with a score of 99 (Neighbor-Joining Tree) and 100 (Maximum Likelihood Tree) and the outgroup of this phylogenetic is Dumortiera hirsuta. Keywords: Marchantia, matK, NCBI, Phylogenetic.
Propagation of Dendrobium crumenatum Sw. through In Vitro Seed Culture Using Fertilizer-Enriched and Organic Complex Media Su'udi, Mukhamad; Ziyaroh, Putry Lazimatu
Life Science and Biotechnology Vol. 2 No. 2 (2024): November 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i2.53562

Abstract

Dendrobium crumenatum Sw. is one of the epiphytic orchids with high ecological and economic value. This study aims to examine the effect of media containing organic compounds enriched with fertilizers for in vitro propagation of D. crumenatum seeds. This culture medium contains organic materials such as potatoes, bananas, coconut water, charcoal, sucrose, and foliar fertilizer. This medium was designed to provide macro, micro, and growth hormone nutrients that support explant growth. The research procedure included sterilization of orchid seeds, explant sowing, and growth observation for about two months. The results showed that orchid seeds experienced optimal growth in media with the addition of coconut water and other organic compounds. The formulation of organic-based media with the addition of coconut water and other supporting compounds is effective in supporting the in vitro growth of orchids, providing opportunities for efficient propagation of orchid species. Explant growth reached 1-2 cm at the 68th day after sowing, with a fresh green colour as an indicator of optimal physiological conditions. The even distribution of shoots indicates that the media provides sufficient space and nutrients without competition between explants. In sum, simple tissue culture media produced from a mixture of organic materials proved reliable function and suited for growing D. crumenatum orchid seeds to produce plantlets, and into further extent it would support conservation efforts. Key words: Conservation, organic materials, pigeon orchid, simple media, tissue culture

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