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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : https://doi.org/10.15294/biosaintifika
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika: Journal of Biology & Biology Education {PISSN 2085-191X| EISSN 2338-7610} published scientific papers on the results of biology and biology education research {see Focus and Scope}. Editor accepts the article has not been published in other media with the writing format as listed on page manuscript writing guidelines {see Author Guidelines}. The journal published three times a year, on April, August & December and published by Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang in collaboration with Perhimpunan Biologi Indonesia.
Arjuna Subject : Ilmu Sosial - Pendidikan
Articles 107 Documents
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Effectiveness of Tuba Root (Derris elliptica) on Histological Structure of Rabbit Fish Liver Sekewael, Usi; Leiwakabessy, Fredy; Warella, Juen Carla; Rahaweman, Ayu Christien; Rumahlatu, Dominggus
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.6754

Abstract

Rabbit fish (Siganus vermiculatus) is one of the biological resources in the sea that is widely consumed by the people in Maluku. Rabbit fish are caught during low tide with nets or by using natural resources to poison the fish, such as tuba root (Derris elliptica), which contains rotenone to make fish easy to catch. This study aimed to determining the concentration of tuba root extract that was effective against damage to the liver tissue of rabbit fish. Rabbit fish and tuba root were collected from Oma Village, Haruku Island, Central Maluku District, Maluku Province.  In the study, a laboratory experiment was conducted in which the tuba root was treated with doses of 0.1 g, 0.3 g, 0.5 g and 1 g with an exposure time of 18 to 24 hours. Data were analyzed using two-way analysis of variance (ANOVA) followed by Duncan's post-hoc test. As a result, the lowest level of liver damage in rabbit fish was observed at a dose of 0.1 g, with a total damage of 7 ± 0.00 and 8.5 ± 0.71. Conversely, the highest level of liver damage was observed at a dose of 1 g, with a total damage of 14 ± 0.00 and 15 ± 0.00. The results of the post hoc test showed a subset value of 0.1 g dose (7.75), 0.3 g dose (9.25), 0.5 g dose (12.00), and 1 g dose (14.50). This research indicates that administering tuba root to rabbit fish can result in damage to the liver's structure. The severity of this damage is contingent upon the dose administered, whereas exposure duration does not affect the level of damage to fish organs.
Knowledge, Attitude, and Practices Toward Snakes and Snakebites among Non-Medical College Students in Cebu, Philippines: A Mixed Method Approach Mutya, Romel; Inocian, Edsel
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.8383

Abstract

Understanding community perceptions and behaviors toward snakes is crucial for effective prevention and management in regions where snakebites are a public health concern. Non-medical college students, a significant demographic, can influence broader community health practices and awareness. This study aims to assess the knowledge, attitudes, and practices (KAP) towards snakes and snakebites among non-medical college students in a technological university in the fifth district of Cebu, Philippines. Employing a convergent parallel research design, quantitative data were collected through structured questionnaires, while qualitative insights were obtained via semi-structured interviews. The sample included 546 non-medical college students selected through random sampling. The findings revealed significant gaps and misconceptions, with students exhibiting moderate knowledge about snakes and snakebites. Attitudes were predominantly negative, characterized by fear and aversion. Practices concerning snakebite management needed to be revised, with many students relying on traditional rather than evidence-based methods. The study highlights considerable areas for improvement in the knowledge and practices of non-medical students regarding snakebite management, coupled with generally negative attitudes toward snakes. The novelty of this research lies in its focus on non-medical college students and specific demographics within a technological university, providing insights that can inform targeted educational interventions. Implementing educational programs to improve KAP among students is recommended, emphasizing correct snakebite first aid and fostering a more informed and balanced view of snakes. This research has significant implications for public health, suggesting that targeted education can enhance community health practices and awareness regarding snakebite prevention and management.
Anti-Inflammatory Effect of Phyllanthus niruri: A Meta-Analysis Susanti, R; Fitriya, Farikhatun Nur; Kristamtini, K; Utomo, Didik Huswo
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.10823

Abstract

Many studies report on the role of Phyllanthus niruri as an anti-inflammatory and there are variations in the results in every research, so a meta-analysis study is needed to obtain accurate results. This study aims to conduct a meta-analysis of the effect of Phyllanthus niruri as an anti-inflammatory. This study uses a meta-analysis research design with data collection from 12 databases. The screening process was carried out according to PRISMA, then analyzed using Comprehensive Meta-Analysis Version 3 (CMA3). Funnel plot and Egger’s test were used as methods to measure publication bias. The articles used were 438 articles from various research journals. After going through screening, as many as 5 articles were eligible to be incorporated in the meta-analysis. Data were analyzed using a random effect model to calculate standardized means difference (SMD). The cytokines analyzed were TNF-α and IL-6 because both were discussed in at least 3 studies. Phyllanthus niruri affected the reduction of inflammatory cytokines with an effect size of SMD on TNF-α is -17,611 (95% CI -29,347, -5,875; p=0.003) and IL-6 is -3,263 (95% CI -5,403, -1,222; p = 0.003). The results of this meta-analysis strengthen the concept/theory that Phyllanthus niruri has the potential as an anti-inflammatory by reducing the inflammatory cytokines TNF-α and IL-6. These results strengthen the scientific basis for the use of Phyllanthus niruri as a supplement for the community to prevent inflammation.
Ethnobotanical Study on Plants Used As Natural Dye by Handwritten Batik Craftsmen in Cirebon Indonesia Jabar, Muhammad Abdul; Rahayu, Enni Suwarsi; Ramasamy, Sujatha
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.10923

Abstract

Cirebon Regency is one of the few Indonesian batik craft center which still use natural dyes in hand-written batik making. The natural dyes are generally extracted from plants. The kind of plants used as natural dyes source for batik in Cirebon Regency are not yet known. This ethnobotanical research aimed to describe the kinds of plants and their characteristics, the frequency of citation by respondents, the parts of the organs used, the sources of obtaining plants, the colors produced, usage technique, as well as the role of using natural dyes in batik to support implementation of green economy. The research approach was exploratory descriptive through observation and interview techniques for data collection. The research was carried out at Kebon Gedang Block, Ciwaringin Village, Cirebon Regency, Indonesia in September 2023. Thirty respondents were selected by purposive sampling method and subjected to the interview. The results showed there were 14 plants species from 12 families used as natural dyes. The most frequently used species were Mangifera indica and Indigofera tinctoria. The organ most widely used was the bark which comes from the purchased, and the color generally produced was brown. The most frequently coloring method used was boiling technique. This research is the first study in this area and useful for providing data and strategic potential for developing local plants as environmentally friendly natural dyes, as well as supporting efforts to use natural dyes in the batik industry.
Cover and Table of Content
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 1 (2024): April 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i1.12138

Abstract

Cover and Table of Content
Cover and Table of Content
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.13940

Abstract

Cover and Table of Content
Inventory of Diseases in Red Shallot Plants (Allium ascalonicum) Haryuni, Haryuni; Setyadi, Ardhanesdian Rizqi; Suprapti, Endang; Dewi, Tyas Soemarah Kurnia; Irawan, Norbertus Citra; Rahman, Azhar Aditya
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 3 (2024): December 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i3.16546

Abstract

Shallots (Allium ascalonicum) are a popular spice vegetable cultivated in Indonesia, ranging from lowlands to highlands. Their demand continues to grow as they are an essential kitchen commodity, although their prices often fluctuate. This research aimed to examine the disease symptoms in shallot plants caused by pathogenic fungi and to identify the types of pathogenic fungi present on agricultural land in Bangsalan Village, Boyolali Regency. The study consisted of three blocks, each containing 12 plots, and each plot comprising 15 plants. From each plot, five plants were randomly selected as samples. This qualitative research utilized observational and descriptive methods, where symptoms observed in the field were described and identified based on macroscopic and microscopic characteristics. Macroscopic identification revealed symptoms such as wilting, rot, and spots on both leaves and tubers. Microscopic identification confirmed infections by the fungi Colletotrichum sp. (causing anthracnose), Fusarium spp., and Aspergillus sp. on shallots. The findings from this study provide valuable recommendations for managing shallot diseases in subsequent planting seasons if similar symptoms are observed.
Cover and Table of Content
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 3 (2024): December 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i3.19878

Abstract

Cover and Table of Content
Response of Eggplant (Solanum melongena) to Diluted Seawater Irrigation Marian S. Dela Cruz; Almoguera, Laurence
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 1 (2025): April 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i1.104

Abstract

The rapid growth of the global population is driving an increasing demand for fresh water, particularly for domestic, industrial, and agricultural use. As freshwater resources become scarcer, exploring alternative water sources for agriculture is imperative to ensure food security. Therefore, this research was conducted to determine whether diluted seawater would impact the growth and productivity of eggplant. The effects of irrigation water with five different salt levels—0, 0.5%, 1.0%, 1.5%, and 2.0% %—were observed on eggplants  (Calixto F1) that were grown in pots for three months. Response variables evaluated included plant height increment, leaf development, stem diameter increase, number of fruits harvested, and unit weight of fruits. The physicochemical properties of the irrigation water and growing media were also tested. The findings revealed that while the overall growth parameters were not significantly affected by the varying salt levels (p>0.05), a notable increase in fruit yield was observed at a 0.5% salinity level. This suggests that a minimal salt concentration in irrigation water can enhance eggplant productivity without compromising plant health. With these findings, this research presents an innovative approach to using diluted seawater as an irrigation strategy for eggplant cultivation. This approach offers a sustainable solution for agriculture in coastal and arid regions where freshwater is limited.
Numeric–Phenetic Study of Lablab purpureus from East Java Based on Morphology Character and DNA Fingerprinting Purwanti, Elly; Wangiyana, I Gde Adi Suryawan; Prihanta, Wahyu; Sukri, Akhmad
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 1 (2025): April 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i1.4604

Abstract

This research aims to conduct a numeric–phenetic study of Lablab purpureus (L) Sweet based on morphology character and DNA fingerprinting with cophenetic–correlation algorithm confirmation. DNA genome extraction is based on the Blood Animal Plant DNA Preparation Kit. Ten RAPD primers were chosen for the amplification DNA genome, including OPA-6, OPA-8, OPA-10, OPA-20, OPC-19, OPD-8, OPD-12, OPE-8, OPE-15, and OPE-16. Cophenetic correlation is used to analyze three clustering algorithms (Nearest Neighbor, UPGMA, Farthest Neighbor) and five similarity indexes (Simple Matching, Jaccard, Nei & Li, Sorensen, Yule). Lablab purpureus (L) Sweet samples have shown morphology variation, mostly on flowers, pods, and seeds. DNA fingerprinting reveals a variety of band numbers ranging from 300BP to 1000BP. RAPD analysis revealed a total of 87 bands, including 18 polymorphic bands, with an average percentage polymorphism of 31.15%. The UPGMA technique was developed as a result of cophenetic-correlation analysis, and the Jaccard similarity index is the optimal combination for dendrogram generation. The topology of a morphology character dendrogram differs slightly from that of a DNA fingerprinting dendrogram. It concluded that the UPGMA algorithm and Jaccard similarity index is the optimum combination to analyze the diversity of L. purpureus (L) Sweet accession from East Java, resulting in the accession of this species having high diversity. This research can give a novel approaching method in the taxonomy field, which is combining morphological and molecular data.

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