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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : https://doi.org/10.15294/biosaintifika
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika: Journal of Biology & Biology Education {PISSN 2085-191X| EISSN 2338-7610} published scientific papers on the results of biology and biology education research {see Focus and Scope}. Editor accepts the article has not been published in other media with the writing format as listed on page manuscript writing guidelines {see Author Guidelines}. The journal published three times a year, on April, August & December and published by Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang in collaboration with Perhimpunan Biologi Indonesia.
Arjuna Subject : Ilmu Sosial - Pendidikan
Articles 107 Documents
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Genetic Diversity of Pacific Seabream, Acanthopagrus pacificus in South-Coast Java, Indonesia Nuryanto, Agus; Bhagawati, Dian; Tuti Winarni, Elly; Wibowo, Dwi Nugroho; Abdalla Mohammed, Mohammed
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.3627

Abstract

Pacific Seabream, Acanthopagrus pacificus, is a popular marine fish from the Sparidae family. This species is an economically essential fisheries commodity in the south-coast Java, Indonesia. However, limited studies are available on A. pacificus in Indonesia. Only two studies reported the presence of A. pacificus in South-Coast Java. No study has been carried out on the biology of A. pacificus from south-coast Java. Therefore, biological studies of A. pacificus are needed, including genetic diversity studies. This study aimed to evaluate the genetic diversity of A. pacificus in South-Coast Java using the cytochrome c oxidase gene. Fish samples were bought from Bantul Fishing Harbor, Yogyakarta, Panganadaran Fishing Port, Bojongsalawe Fish Auction Center, West Java, and Binuangeun Fishing Harbor in Lebak Regency, Banten. The genetic marker was processed in PT. Genetika Science Indonesia follows the company procedure. Haplotype and nucleotide diversity were calculated mathematically using Arlequin software. The result showed that polymorphic loci were only 1.3%, indicating low polymorphisms. A. pacificus showed haplotype and nucleotide diversities of 0.511±0.110 and 0.15%±0.12%, respectively. Those values indicated low genetic diversity. This study concluded that the A. pacificus population in South-Coast Java showed low genetic diversity. This study provides the first data about the genetic diversity of A. pacificus in South-Coast Java, which is essential data for fisheries management.
The potency of Elaeocarpus grandiflorus Leaf Extract as Anti-obesity:  in vivo and in silico Study Nugrahaningsih, WH; Ramasamy, Sujatha; Laraswati, Vivi Anggraeni; Yuniastuti, Ari; Christijanti, Wulan
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.3695

Abstract

The research aims to determine the effect of ethanol extract of Elaeocarpus grandiflorus leaves extract as anti-obesity by in vivo and silico computational models.  The pre-and -post-tests were carried out on 20 female Wistar rats, divided into 4 groups. The control group (K) received no treatment and the experimental groups were treated with E. grandiflorus extract of 200 mg/kg BW (P1), 400 mg/kg BW (P2), and 800 mg/kg BW (P3) for 14 days. The body weight, abdominal circumference, and abdominal fat mass were measured on Day 1 and Day 15. The results showed differences in body weight (p=0.02), abdominal circumference (p=0.01), and abdominal fat mass (p=0.00). In silico exploration, bioactive compounds of rutin, orientin, luteolin, vitexin, iso orientin, isovitexin, kaempferol, and quercetin were identified, and targeted ELAVL1, IGF1R, CREB1, AKT1, and PIK3R1 of the AMPK signaling pathway that involved in the anti-obesity mechanism. The high binding affinity values was rutin-EVAL1 (-9.3), orientin-ELAVL1 (-8.3), and quercetin- IGFR1 (-8.2). It can be concluded that the ethanol extract of E. grandiflorus leaves has the potential to be developed as an anti-obesity agent.
Unveiling the Enigmatic Dwarf Horseface Loach Acanthopsoides molobrion: A Groundbreaking Discovery in Indonesia Fitri Sil Valen; Denny Syaputra; Ismail, Mohd Hasmadi; Swarlanda, Swarlanda; Kamarudin, Ahmad Syazni; Veryl Hasan; I, Itaya
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.5671

Abstract

Acanthopsoides molobrion is a freshwater previously known to be spread across Malaysia and Borneo. This study aimed to record for the first time the occurrence of the A. molobrion in Bangka Island, Indonesia, and update the geographic distribution of this species. This new record of freshwater fish provides an important contribution to the comprehension of the biogeography of the species. The purpose sampling approach was employed for this study to collect specimens. The species were then identified morphologically by the application of morphometric and meristic methods, and molecularly through the employ of DNA Barcoding. On 20th January 2023, two A. molobrion specimens were collected from the Bumang Kemuja River, Bangka Island using a fish trap. The new record of A. molobrion found here is the southernmost record for this species, expanding its geographic distribution. In addition, the latest record site is about 500 km south of the nearest locality in Peninsular Malaysia, and about 750 km southwest of the nearest locality in Borneo. The new record of A. molobrion has expanded the species' recorded distribution range, which has added to our understanding of this species. Furthermore, we present an updated record for the A. molobrion DNA sequence based on the COI gene. This sequence is the first DNA Barcode to Indonesia. Subsequently, the DNA sequence was registered into NCBI Genbank with the access code OR144414. This DNA barcode will be used as a standard for identifying A. molobrion and will aid in DNA and biotechnology-based studies in the future.
The Role of Sembung (Blumea balsamifera) Leaf Extract in Preventing Atherosclerosis in Hyperlipidemia Rat Models I Made Jawi; I Wayan Putu Sutirta Yasa; I Gede Widhiantara; Anak Agung Ngurah Subawa; Putu Angga Wiradana; Naw, Sin War
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.6673

Abstract

This study aims to prove that administering BBLE as a natural antioxidant can prevent atherosclerosis by maintaining lipid profiles, antioxidant enzymes, and netrin-1 levels in hyperlipidemia in rat models. The research subjects were 20 adult male Wistar rats (Rattus norvegicus), which were divided into 2 groups using a randomized pretest and posttest control group design. Before treatment and after treatment for 3 months, lipid profiles, MDA, SOD, and netrin-1  were examined. The control group was only given high-cholesterol diets (HCD), while the treatment, apart from HCD, was also given BBLE 4mg/day. The data obtained was tested using paired t-test and group t-test. The results of the study showed that there was a significant decrease in netrin-1 in the control group (p<0.05) after being given HCD for three months. In the treatment group, it also decreased but it was not significant (p>0.05). Netrin-1 levels in the treatment group were higher than the control (p<0.05). The lipid profile experienced a significant increase in HDL in the treatment group accompanied by a significant decrease in MDA and an increase in SOD (p<0.05) when compared with the control group. This study concludes that administering BBLE at a dose of 4 mg/day to rats given HCD caused an increase in netrin-1 levels accompanied by improvements in lipid profiles and prevention of oxidative stress. The findings of this study reveal the novelty of BBLE in treating and maintaining blood vessel function in mice given HCD by increasing netrin-1 levels.
Antihyperlipidemic Activity of Tamarind (Tamarindus indica) Extract on the Lipid Profile of Albino Rats Christijanti, Wulan; Susanti, R.; Rakainsa, Senda Kartika; Widyaningrum, Kartika; Mohamed, Mohd Salim Bin
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 1 (2024): April 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i1.7579

Abstract

Tamarind are widely used as antioxidants, anti-diabetes, anti-hyperlipidemia, and anti-cholesterolemia. The aim of this research is to analyze the anti-hyperlipidemic activity of extracts of tamarind fruit pulp, seeds and leaves on the lipid profile of rats. Experimental research was designed with 20 male rats divided   Control (C), Tamarind leaves (TL), Tamarind fruit pulp (TF), and Tamarind seeds (TS) extracts, each 500 mg/kg of body weight. All of the groups were given standard feed and induced with lard at 3 ml/rat/day for 14 days. On day 15, the rats were continued to be induced with lard and were given extracts according to each group for 2 weeks. The data obtained such as rats’ body weight, level of cholesterol, HDL (high-density lipoprotein), LDL (low-density lipoprotein), triglycerides (TG) and Malondialdehyde (MDA)  were analyzed using anova (P<0.05) and the Duncan Multiple Range Test (DMRT). The results showed that each extract had a significant effect on cholesterol, HDL and MDA levels but not on body weight, LDL and triglycerides. Levels of MDA in the group with the extract were significantly lower than the control. Tamarind pulp, seeds and leaf extracts show antihyperlipidemic activity by reducing cholesterol and HDL levels. This can be the basis for using parts of the tamarind plant as a supplement for the community to maintain fat levels.
Antibacterial Production by Endophytic Bacteria from Catharanthus roseus in East Timor Against Methicillin-Resistant Staphylococcus aureus  Missa, Hildegardis; Ndukang, Sardina; Djalo, Aloysus; Nau, Getrudis W.; Susilowati, Ari; Baunsele, Anselmus Boy; Santos, Americo Dos
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.7675

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) refers to a group of bacteria that cause infections and present a challenge in treatment due to their resistance to several antibiotics. Therefore, research is needed to discover endophytic bacteria from Catharatus roseus capable of producing new antibiotics. This study aims to identify endophytic bacteria from C. roseus plants originating from Timor Island, as producers of effective antibacterial compounds against MRSA. Antibacterial activity tests were conducted on two types of test bacteria, namely MRSA and Escherichia coli. DNA extraction was performed using the PrestoTM Mini dDNA kit Bacteria, and amplification of the 16S rRNA gene was carried out. The research found bacterial isolates showing morphological similarities to Bacillus sp. Screening results indicated that four bacterial isolates exhibited high potential antibacterial activity against MRSA, as evidenced by the formation of inhibition zones with diameters of approximately 25 mm-35 mm. Amplification of the four endophytic bacterial isolates from C. roseus identified them as Paenibacillae FaCH2, Bacillae BoCH3, Aneurinibacillae BoCH5, Aneurinibacillae BiCH8, which are new species based on 16S rRNA gene similarity of less than 97%. In conclusion, endophytic bacteria from C. roseus producing antibacterial compounds against MRSA have been successfully identified. This is beneficial to society because the antibacterial compounds produced can serve as a basis for developing new drugs that are effective against MRSA infections, considering the increasing antibiotic resistance against MRSA.
Content Analysis of Bioenergetics’ Conceptual Presentation in Senior High School Biology Textbooks Alforque, Jonemel M.; Jovero, Marijul B.; Bodanio, Cenando; Ecle, Niño; Picardal, Jay P.
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.3399

Abstract

Factors that bring about a low mastery level of bioenergetics, a core concept in biology, include relatively poor teaching-learning experience and low-quality textbooks, among others. To determine which textbook and teaching components have affected the low learning mastery of the topics in bioenergetics, the researchers analyzed the textbooks’ quality of content, language, layout, and figures, and the teachers’ experience, contentment, and challenges while teaching the topic, and effects to the learners. Using a sequential explanatory mixed method research design, ten (10) textbooks, and seven (7) teacher participants were purposively selected following a set of criteria. Descriptive and principal component analyses revealed that the textbooks' quality depends on two components: (1) competent and coherent visuals and content, and (2) consistency of style in the varied forms of assessment. Narrative analysis and Quasi-Statistics analysis revealed that teachers are not contented with the textbooks as they are inadequate to teach the subject matter. The lack of good learning materials, coupled with low competence to introduce the topics, resulted in the low mastery level of the learners. The researchers propose that periodic textbook assessment, retooling of teachers’ skills, and deepening teachers’ understanding are necessary to improve learners’ performance in bioenergetics. Finally, this paper is instrumental in providing educators, resources and curriculum developers, and researchers a feasible and practical path to improve textbook quality and address pedagogical gaps.
The Potential of Deleterious rhizobacteria to Control Primary Weed Echinochloa sp. on Rice Hoesain, Mohammad; Masnilah, Rachmi; Nurchayanti, Suhartiningsih Dwi; Pradana, Ankardiansyah Pandu; Alfarisy, Fariz Kustiawan; Sari, Dwi Tirta; Ramadhan, Farchan Mushal Al; Rismayani; Tarigan, Sri Ita
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.3422

Abstract

Deleterious rhizobacteria (DRB) is one of the rhizobacteria that can be used as a bioherbicide and can stimulate plant growth. The objectives that can be obtained from this study are to determine the potential of DRB on the growth of weeds Echinochloa crusgalli and Echinochloa colona on rice. Variables observed in this study were identification of secondary metabolites, measurement of weed sprouts, percentage effectiveness of bacterial isolates against weed seeds, normal sprouts, abnormal sprouts, fresh seeds that did not grow and dead seeds. The aim of this study is to explore biological agents that can be used to control the main weeds of rice crops. This candidate could be a recommendation for environmentally friendly controls. Exploration of the DRB group was obtained by collecting soil samples around the roots of rice crops. Then isolate it, identify as morphologically and physiologically, and test it in vitro studies. The results showed that DRB had the potential to control weed growth and could stimulate the growth of rice. The application of PFA and PFB isolates had a significant effect on inhibiting the length of weed roots and buds, the average percentage of control effectiveness > 80-90%. Novelty in this study could be found as candidate groups of DRB bacteria with specific locations to control the weed Echinochloa sp. on rice crops. For society, this research could be an alternative to reduce dependence on synthetic herbicides . DRB has the potential to be formulated into a bioherbicide  to control weeds.
Above Ground Biomass and Carbon Stock at Pesanggrahan Preserve Area, Malang, East Java, Indonesia Kundariati, Maisuna; Rohman, Fatchur; Ibrohim, Ibrohim; Nida, Safwatun; Abdul Razak, Sarah; Fardhani, Indra
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.3485

Abstract

Tropical forests have known ecosystem services, including sequestering atmospheric carbon and fixing it into biomass. Since the revolutionary industrial changes, the atmospheric carbon level has risen during the recent decades, while the global temperature has increased compared to the pre-industrial levels. Furthermore, reducing atmospheric carbon can mitigate global warming and climate change. This study aims to estimate the aboveground biomass and carbon stock in the Pesanggrahan Forest, Malang Regency, East Java, Indonesia, and contribute local information to the vast global literature. This study was conducted in August 2023 and measured all tree species with a diameter >20 cm in a 5-ha area. The results found 208 individuals and 39 species of trees, the aboveground biomass and carbon stock of which were calculated. The total aboveground biomass (AGB) and carbon stock were 4,486 and 2,248 tons C/ha, respectively. Meanwhile, the total forest AGB and carbon stock in the 5-ha sampling area were 224.77 tons/ha and 112.39 tons C/ha, respectively. These findings highlight the cruciality of tropical forest vegetation in carbon storage and their overall role in regional and global ecosystem management in light of climate change.
Therapeutic Potential of Secretome Hypoxia Mesenchymal Stem Cells: Downregulation of TNF-α and HIF-2α in Metabolic Syndrome-Induced Inflammation in Wistar Rats Dewi, Alisia Martha; Trisnasi, Setyo; Putra, Agung; Chodijah , Chodijah; Sarosa, Hadi; Mulyani, Sri Priyantini; Amalina, Nur Dina; Ibrahim, Sugeng
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.6613

Abstract

Background: Metabolic syndrome (MetS) has become a global health challenge with several associated issues, such as obesity, insulin resistance, dyslipidemia, and hypertension. Important proteins such as Tumor Necrosis Factor-alpha (TNF-α) and Hypoxia Inducible Factor-2 alpha (HIF-2α) regulate the inflammatory process by inducing the expression of pro-inflammatory proteins. Secretome Hypoxia Mesenchymal Stem Cells (SH-MSCs) are immunomodulatory, anti-inflammatory, and angiogenic stimulators, which can regulate various inflammatory diseases, including MetS. Objective: This study aims to determine the effect of administering SH-MSCSs on the expression of the TNF-α and Hypoxia Inducible Factor (HIF)-2α genes in the male Wistar rat model with Metabolic Syndrome. Method: This research is an experimental study with a Post-test Only Control Group Design, using a total of 24 male Wistar rats divided into four groups: T1 (Healthy control), T2 (MetS + NaCl), T3 (MetS + administration of SH-MSCs dose 150 uL), and T4 (MetS + administration of SH-MSCs dose 300 uL). SH-MSCSs were administered intraperitoneally four times over 14 days. Adipose tissue TNF-α and HIF-2α gene expression were measured on day 15 using qRT-PCR. Results: TNF-α and HIF-2α gene expression was significantly lower in T3 and T4, compared with the MetS control group (T2). Conclusion: Administration of SH-MSCs was able to reduce the expression of the Tumor Necrosis Factor (TNF-α) and Hypoxia Inducible Factor (HIF)-2α genes in fatty tissue in the male Wistar rat model with Metabolic Syndrome.

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