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Jurnal Sains dan Kesehatan
Published by Universitas Mulawarman
ISSN : 23030267     EISSN : 24076082     DOI : 10.25026/jsk
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology
Jurnal Sains dan Kesehatan menerima naskah karya asli (Artikel Penelitian, Artikel Review, dan Studi Kasus), baik eksperimental maupun teoretis, dalam bidang-bidang berikut: Sains dan Kesehatan (Kedokteran, Farmasi, Kesehatan Masyarakat, Keperawatan, Kebidanan, Gizi, dan lain-lain). Tujuan penerbitan Jurnal Sains dan Kesehatan adalah untuk menyediakan informasi ilmiah tentang perkembangan ilmu dan teknologi di bidang Ilmu Pengetahuan dan Kesehatan.
Arjuna Subject : Pharmacology, Toxicology and Pharmaceutics - Ilmu Farmasi
Articles 764 Documents
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Validasi Metode Penetapan Kadar Pengawet Natrium Benzoat pada Sari Kedelai di Beberapa Kecamatan di Kabupaten Tulungagung Menggunakan Spektrofotometer Uv-Vis Siti Awwalul Amanatur Rohmah; Afidatul Muadifah; Rahma Diyan Martha
Jurnal Sains dan Kesehatan Vol. 3 No. 2 (2021): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v3i2.265

Abstract

Sodium benzoate is an organic preservative chemical compound which use is permitted if the amount is below the maximum threshold. This research aims to validate the UV-Vis Spectrophotometry method which will be used to determine sodium benzoate levels in soybean milk in 3 sub-districts in the Tulungagung Regency using the UV-Vis Spectrophotometer instruments. Before determining the content, the samples were analyzed qualitatively first using the acid-base titration method. The result of this qualitative analysis of the sample will turn pink if it contains sodium benzoate. Then the sodium benzoate wavelength optimization is carried out in the range of 200-400nm, and the optimum wavelength is 226nm. The method validation process is done by using four parameters namely linearity test, accuracy test, precision test, and LOD&LOQ. Based on the validation of the method, the correlation coefficient (R2) of 0.99563 indicates linear, recovery % is 97.58% in the range of 80-120%, RSD is 0.0454% which is ?2%, LOD is 0.33 ppm, and LOQ of 1.0996 ppm. Based on these results, it can be said that the method used is valid because all parameters meet the specified requirements. Analysis of the determination of levels using a UV-Vis Spectrophotometer instruments at wavelength 226nm using 5 samples, from the five samples obtained average rate of 90.639±0.0406. Analysis of the rate determination using UV-Vis Spectrophotometer instruments at 226nm wavelengths show that sample A has a concentration 92,243±0,039 ppm, sample B is 80,286±0,039 ppm, sample C is 99,04±0,063 ppm, sample D is 101,483±0,025 ppm, and sample E is 80,143±0,038 ppm. The content of sodium benzoate in soybean milk is following the requirements of BPOM RI regulation No.36 of 2013 concerning the maximum limit of food use, the use of sodium benzoate in fruit/vegetable juice products and non-fermented soybean products is 600ppm of food, with ADI 0-5mg/kg body weight.
Aktivitas Anti Candida albicans ATCC 14053 Sediaan Pasta Gigi Gel Ekstrak Daun Jengkol (Archidendron pauciflorum) dengan Kombinasi Na-CMC dan Karbomer: Activity of Anti-Candida albicans ATCC 14053 Toothpaste Gel Leaf Extract Jengkol (Archidendron pauciflorum) with Combination of Na-CMC and Carbomer Aria Agustina Achsia; Ary Kristijono; Dara Pranidya Tilarso
Jurnal Sains dan Kesehatan Vol. 3 No. 2 (2021): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v3i2.267

Abstract

Jengkol leaf extract can inhibit the growth of the fungus Candida albicans because it contains flavonoids, saponins, and tannins. Candida albicans species are most commonly found in the oral cavity that can cause various diseases in humans as canker sores and skin lesions. The purpose of this study was to formulate jengkol leaf extract into gel toothpaste preparations by distinguishing variations in the concentration of carbomer as a gelling agent. The research method used is experimental. Jengkol leaf samples extracted using the maceration method with 70% ethanol. The concentration of active substance used in each formula was 7.5% based on the preliminary test results and the carbomer concentration used was 0.5% in formula I, 1% in formula II, 1.5% in formula III, and 2% in formula IV. The physical stability test consists of organoleptic, homogeneity, spreadability, viscosity, pH, and high foam for 28 days of storage. Antifungal activity test using the disc diffusion method. Statistic analysis performed using the Shapiro-Wilk, Levene Statistics, and One Way Anova methods. During 28 days of keeping all formula homogeneous stable. The viscosity increased in each formulation due to differences in the concentration of carbomer used, the higher of carbomer the more viscous of the preparation, the pH, foam height of the formulation was stable and met the standard. The particles are evenly distributed so that the formulation colors and odors have stable. The result of the antifungal activity test of toothpaste gel extract of 7,5% jengkol leaf has inhibition of 11 mm. The increase in carbomer concentration showed a significant difference in each formula based on the One Way Anova statistical analysis (p <0.05) and the addition of carbomers to the formula showed stable viscosity and high foam. The most stable formula is the formula I with a carbomer concentration of 0.5%.
Aktivitas ANtibakteri Fraksi Daun Kakao (Theobroma cacao L.) terhadap Bakteri Staphylococcus aureus Secara In Vitro: Antibacterial Activity of Cocoa Leaf Fraction (Theobroma cacao L.) against Staphylococcus aureus Bacteria by In Vitro Noviana Mandhaki; Choirul Huda; Amalia Eka Putri
Jurnal Sains dan Kesehatan Vol. 3 No. 2 (2021): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v3i2.269

Abstract

Cacao are a plantation commodity in Indonesia. Most of the cacao plant is cacao leaves. One of the utilization of cacao leaves by the community is as compost, but has not been utilized by the community as a raw material of traditional medicine. Based on research, cacao leaves contain secondary metabolite compounds that can be used as an antibacterial against Staphylococcus aureus bacteria that often cause infection in humans. The purpose of this study was to find out the activity of ethanol fraction, dichloromethane, and n-hexane from cacao leaf extract as an antibacterial against Staphylococcus aureus ATCC 25923. This study used extraction methods of maceration and fractionation using ethanol solvents, dichloromethane, and n-hexane. Test the antibacterial activity of cacao leaf fraction using disc diffusion method with a concentration series of 10%, 20%, and 30%. The results showed that ethanol and dichloromethane fractions had antibacterial activity.
Uji Aktivitas Antibakteri Fraksi Daun Eceng Gondok (Eichhornia crassipes) terhadap Bakteri Staphylococcus aureus: Antibacterial Activity Test of Water Hyacinth Leaf Fraction (Eichhornia crassipes) against Staphylococcus aureus Bacteria Shindy Charisma Nur Qur’an; Choirul Huda; Rahma Diyan Martha
Jurnal Sains dan Kesehatan Vol. 3 No. 2 (2021): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v3i2.270

Abstract

Staphylococcus aureus merupakan bakteri Gram positif yang memiliki habitat alami pada manusia dan mudah resisten terhadap antibiotik. Alternatif yang lebih aman untuk mengatasi masalah infeksi Staphylococcus aureus yaitu dengan memanfaatkan bahan aktif antimikroba dari tanaman obat. Salah satu tanaman yang dapat digunakan adalah eceng gondok. Eceng gondok merupakan tanaman yang memiliki kemampuan mengahambat aktivitas bakteri. Tujuan penelitian ini adalah untuk mengetahui aktivitas antibakteri maserat ekstrak, fraksi teraktif dari fraksi etanol 96%, fraksi diklorometana, fraksi N-heksan dan konsentrasi optimum fraksi aktif daun eceng gondok terhadap bakteri Staphylococcus aureus. Daun eceng gondok diekstraksi menggunakan metode maserasi dengan pelarut etanol 96% dan dilakukan proses fraksinasi menggunakan pelarut etanol, diklorometana dan N-heksan. Uji aktivitas antibakteri dilakukan metode difusi cakram kertas. Fraksi teraktif dilakukan seri konsentrasi 15%, 30%, dan 45%. Hasil uji aktivitas antibakteri menunjukkan maserat ekstrak daun eceng gondok memiliki aktivitas antibakteri dengan diameter rata-rata 10,3 mm. Fraksi etanol merupakan fraksi teraktif yang memiliki aktivitas antibakteri terhadap bakteri Staphylococcus aureus dengan hasil rata-rata diameter 21 mm. Konsentrasi optimum dari fraksi teraktif adalah konsentrasi 15% dengan diameter rata-rata 13,67 mm
Perbedaan Nilai Fraksi Ejeksi Ventrikel Kiri (LVEF) Pasien IMA-EST yang Menjalani Terapi Reperfusi dengan Fibrinolitik dan IKPP di RSUD Abdul Wahab Sjahranie Samarinda: Difference in Left Ventricular Ejection Fraction (LVEF) Value of AMI-EST Patients Underwent Reperfusion Therapy with Fibrinolytics and IKPP at Abdul Wahab Sjahranie Hospital, Samarinda Annisa Fitri Fadhilah; Djoen Herdianto; Muhammad Khairul Nuryanto
Jurnal Sains dan Kesehatan Vol. 3 No. 2 (2021): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v3i2.271

Abstract

Patients with Acute Myocardial Infarction with ST Segment Elevation (STEMI) should be given reperfusion therapy with fibrinolytic or PPCI (Primary Percutaneous Coronary Intervention) to prevent further myocardial damage. Currently PPCI is the main therapy choice for STEMI patients, but not all health facilities have these facilities. Thus, fibrinolytic may be the reperfusion therapy of choice in patients STEMI with chest pain onset ? 12 hours and without contraindications. STEMI can affect heart function, so the LVEF (Left Ventricular Ejection Fraction) value can be used to assess heart systolic function which can affect the prognosis of STEMI patients. The aim of this study was to identify the difference of LVEF values ??between STEMI patients undergoing reperfusion therapy with fibrinolytic and PPCI at Abdul Wahab Sajahranie Hospital, Samarinda. This study was an analytic observational study with a cross sectional design. The study population was all STEMI patients who undergoing reperfusion therapy with fibrinolytic and PPCI at Abdul Wahab Sjahranie Hospital, Samarinda for the period 2018-2019. Sampling was purposive sampling, with data sources derived from the patient's medical records. LVEF values ??were examined using transthoracic echocardiography, and Independent T-Test was used to analyze the difference of LVEF values ??between these groups. The result showed there was no significant difference in the LVEF value of STEMI patients undergoing reperfusion therapy with Fibrinolytics and PPCI (fibrinolytic 51.37 ± 12.254%, PPCI 56.47 ± 11.98%; p = 0.103 (p> 0.05)). Keywords: STEMI, Fibrinolytic, PPCI, LVEF
Pengaruh Variasi Konsentrasi Karaginan Kombinasi Emulgator Anionik dan Nonionik Terhadap Formulasi Losion Astaxanthin Ekstrak Minyak Cincalok Nia Risnawati; Siti Nani Nurbaeti; Hadi Kurniawan
Jurnal Sains dan Kesehatan Vol. 3 No. 2 (2021): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v3i2.272

Abstract

ABSTRACT Cincalok is a typical food of West Kalimantan, Indonesia. Cincalok is made from the fermentation of rebon shrimp, which contains astaxanthin compounds inside them. Astaxanthin has strong antioxidant properties with an IC50 value of 18.75 ppm. Astaxanthin from cincalok oil extract can be formulated into a lotion for skin care. Lotion were made in 4 formulas. Formula F1 and F2 used a combination of anionic emulgators (stearic acid and TEA), formulas F3 and F4 used a combination of nonionic emulgators (Span 60 and Tween 60). Concentration carrageenan as a viscosity agent was 0.75% in F1 and F3, and concentration of 1% in F2 and F4. All formulas were tested for their physical properties such as other organoleptic test, homogenity, spreadibility, adhesion, pH , viscosity, and globul size. Lotion were also tested for astaxanthin levels contained in lotion. The results showed that the formula F3 was the best formula based on physical properties and astaxanthin levels test in lotion. Keywords: Astaxanthin, Anionic, Cincalok, Lotion, Nonionic
Hubungan Usia, Jenis Kelamin, dan Status Perdarahan terhadap terjadinya Komplikasi pada Anak dengan Infeksi Dengue di RSUD Abdul Wahab Sjaharanie Samarinda: Relationship of Age, Gender, and Bleeding Status to the occurrence of Complications in Children with Dengue Infection at Abdul Wahab Sjaharanie Hospital, Samarinda Regita Pratiwi; Yuniati Yuniati; Muhammad Buchori
Jurnal Sains dan Kesehatan Vol. 3 No. 2 (2021): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v3i2.274

Abstract

Beberapa studi mendapatkan hasil bahwa pada anak dengan infeksi dengue dapat ditemukan komplikasi dengan beberapa faktor yang mempengaruhi. Penelitian ini bersifat retrospektif dengan tujuan untuk mengetahui ada tidaknya hubungan antara usia, jenis kelamin dan status perdarahan terhadap terjadinya komplikasi pada anak dengan infeksi dengue di RSUD Abdul Wahab Sjahranie Samarinda. Penelitian ini menggunakan desain cross sectional. Sampel pada penelitian ini adalah 110 rekam medis dengan menggunakan teknik purposive sampling. Analisis dilakukan dengan uji Chi Square. Hasil penelitian ini menunjukkan 36 orang (32,7%) mengalami komplikasi. Status perdarahan memiliki hubungan yang signifikan terhadap terjadinya komplikasi (p = 0,026, OR 2,52, IK 95%; 1,10;5,76), usia (p = 0,799) dan jenis kelamin (p = 0,347) tidak berhubungan dengan terjadinya komplikasi. Berdasarkan hasil penelitian, dapat disimpulkan bahwa status perdarahan berhubungan dengan terjadinya komplikasi pada anak dengan infeksi dengue di RSUD Abdul Wahab Sjahranie Samarinda. .
Insidensi dan Karakteristik Karsinoma Hepatoseluler di RSUD Abdul Wahab Sjahranie Samarinda: Incidence and Characteristics of Hepatocellular Carcinomas in RSUD Abdul Wahab Sjahranie Samarinda Dinda Aprilistya Puri; Sinta Murti; Yudanti Riastiti
Jurnal Sains dan Kesehatan Vol. 3 No. 2 (2021): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v3i2.275

Abstract

Abstract Hepatocellular carcinoma (HCC) is a primary liver cancer that resulted from abnormal hepatocyte proliferation. HCC ranked fifth in the incidence of cancer cases and was the fourth leading cause of cancer death in the world in 2018. HCC patients have a poor prognosis and a low survival rate. This study aims to discover the incidence and characteristics of hepatocellular carcinoma in Abdul Wahab Sjahranie Regional Public Hospital Samarinda. The study design is a retrospective descriptive study. Data were obtained from patients' medical records data from February 2017 – February 2020. The result of this study were 206 HCC patients. From 206 patients, 82,5% were male and the oldest age group was 50-59 years old (33,5%); Hepatitis B (62,6%) was the most prevalent risk factor; 60,7% patients showed an increased level of AFP; 43,7% matched Child-Pugh B classification; 22,3% showed stage B BCLC; the most commonly used therapy was no-intervention therapy (93,2%). Survival rate was significantly affected by Child-Pugh classification (p=0,03) and BCLC stage (p=0,025). According to this study result, we can conclude that HCC incidence in the last 3 years is still high; HCC patients were predominantly male than female; the oldest age group was 50-59 years old; the most prevalent risk factor was Hepatitis B; HCC patients were more likely to develop increased AFP level; the most prevalent Child-Pugh classification was Child-Pugh B; the most prevalent stage was stage B and; 1-year survival rate was affected by Child-Pugh classification and BCLC stage. Keywords: Hepatocellular carcinoma, incidence, characteristics, the survival rate Abstrak Karsinoma hepatoseluler (KHS) merupakan kanker hati primer yang terjadi akibat tumbuhnya sel hepatosit secara abnormal. KHS menduduki peringkat kelima insidensi kasus kanker terbanyak dan urutan keempat penyebab kematian akibat kanker di dunia tahun 2018. Pasien KHS memiliki prognosis yang buruk dan survival rate yang rendah. Penelitian ini bertujuan untuk mengetahui insidensi dan karakteristik karsinoma hepatoseluler di RSUD Abdul Wahab Sjahranie Samarinda. Penelitian ini merupakan penelitian deskriptif retrospektif. Data yang digunakan dalam penelitian ini diperoleh dari data rekam medik pasien periode Februari 2017-Februari 2020. Hasil penelitian ini terdapat 206 pasien KHS. Dari 206 pasien 82,5% adalah laki-laki dengan kelompok usia tertinggi 50-59 tahun (33,5%), hepatitis B (62,6%) menjadi faktor risiko terbanyak, 60,7% pasien mengalami peningkatan level AFP, 43,7% menunjukkan klasifikasi Child-Pugh B, 22,3% menunjukkan stadium BCLC B, terapi paling banyak diberikan adalah terapi tanpa intervensi (93,2%). Survival rate secara signifikan dipengaruhi oleh klasifikasi Child-Pugh (p=0,017) dan stadium BCLC (p=0,037), tetapi survival rate tidak dipengaruhi oleh modalitas terapi (p=0,92). Berdasarkan penelitian ini dapat disimpulkan bahwa insidensi KHS pada 3 tahun terakhir masih tinggi, pasien KHS laki-laki lebih banyak daripada perempuan, kelompok usia tertinggi adalah kelompok usia 50-59 tahun, faktor risiko terbanyak adalah hepatitis B, pasien KHS lebih banyak mengalami peningkatan level AFP, klasifikasi Child-Pugh terbanyak adalah Child-Pugh B, stadium dengan jumlah terbanyak adalah stadium B, dan survival rate 1 tahun dipengaruhi oleh klasifikasi Child-Pugh dan stadium BCLC. Kata kunci : Karsinoma hepatoseluler, insidensi, karakteristik, survival rate
Review: Analisis Komponen Senyawa Kimia Krokot (Portulaca oleraceae L. dan Portulaca grandiflora Hook.): Review: Component Analysis of Purslanes Chemicals Compound (Portulaca oleraceae L. andPortulaca grandiflora Hook.) Sri Gustini Husein; Melvia Sundalian; Nurul Husna
Jurnal Sains dan Kesehatan Vol. 3 No. 2 (2021): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v3i2.278

Abstract

Purslanes are medicinal plants from the Portulacaceae family which has more than 100 species, including Portulaca oleraceae L. and Portulaca grandiflora Hook. Both types of purslanes have the potential to become natural medicines due to the presence of active chemical compounds that produce various pharmacological effects. This review compiled the data concerning chemical compounds found in those two types of purslanes, the method of analysis, as well as the factors that affect the quality and quantity of chemical compounds so that they can be used as references in determining natural medicinal ingredients by selecting purslanes based on their desired phytochemical compounds and the appropriate analysis. The data were collected by a method of searching research journals on Google Scholar using certain keywords. From this review, the results showed that the chemical compounds found in the two types of purslanes are phenolic, flavonoid, fatty acid, sterol, terpenoid, and alkaloid. These components can be analyzed using spectroscopic and chromatographic methods for both qualitative and quantitative analysis. Some factors that need to be considered in the analysis of these components are the analytical method used, the method of extraction, the solvents, the plant varieties and genotypes, the part of the plant used, the growth time and harvest time of the sample, and the conditions in which the samples grew.
Aktivitas Analgetika dan Antiinflamasi Ekstrak Etanol dan Fraksi Daun Jambu Mawar (Syzygium jambos L.) Secara In Vivo: Analgetic and Anti-inflammatory Activity of Ethanol Extract and Fraction of Guava Leaf (Syzygium jambos L.) In Vivo Deden Winda Suwandi; Tita Puspita; Doni Anshar Nuari; Syifa Hamdani
Jurnal Sains dan Kesehatan Vol. 3 No. 2 (2021): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v3i2.279

Abstract

Syzygium jambos is a plant that has traditionally been used to treat several diseases, especially the leaves as a medicine for rheumatism. In this study, the ethanol extract, fraction of n-hexane, ethyl acetate, and water from its leaves were tested for analgesic and anti-inflammatory activity using the writhing method (Siegmund), hot plate, and the formation of swelling of the soles induced by carrageenan lamda. The results showed that the ethanol extract at doses of 100 and 200 mg/kg BW and fractions of n-hexane, ethyl acetate and water at doses of 50 and 100 mg/kg BW had analgesic and anti-inflammatory activities. The ethanol extract at doses of 100 and 200 mg/kg BW can reduce the amount of writhing every 5 minutes for 60 minutes of observation by 84 and 98 times with percent protection of 58.0 and 52.0%. The Ethyl acetate, n-hexane, and water fractions of 50 and 100 mg/kg BW can reduce the amount of animal writhing by 93 and 35 times, 75 and 100 times, as well as 84 and 124 times with the percent of protection is 54.45 and 82.85, 63.0 and 50.9, as well as 58.47 and 38.02%. The analgesic activity of the hot plate method, ethanol extract can increase the survival time of animals in the hot plate by 154.0 and 95.02%, while the animal survival time of the n-hexane fraction is 95.86 and 105.5, ethyl acetate by 143, 7 and 105.7, and the water fraction of 34.1 and 99.5%. The anti-inflammatory activity of ethanol extract at doses of 100 and 200 mg/kg BW was indicated by a decrease in the volume of rat leg edema at the time of observation for 6 hours with the percent inhibition of edema of 59.2 and 41.12%. The n-hexane, ethyl acetate, and water fractions have relatively the same ability, indicated by the edema inhibition value of the n-hexane fraction of 68.24 and 68.56%, the ethyl acetate fraction of 57.08 and 67.32% and the fraction water of 64.02 and 67.46%. The effective dose of extracts and fractions of n-hexane, ethyl acetate, and water as analgesics and anti-inflammatory is 100 mg/kg BW.

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