Genbinesia Journal of Biology
The Genbinesia Journal of Biology particularly focuses on the core disciplines in biology, they are: astrobiology, bacteriology, behavioural science, biochemistry, biodiversity, bioethics, bioinformatics, biomathematics, biophysics, biopolymers, biostatistics, biotechnology, botany, cell biology, conservation, crystallography of macromolecules, developmental biology, ecology, entomology, evolutionary biology, genetics, genomics, glycobiology, immunology, macromolecules, marine & aquatic biology, mathematical & computational biology, medicine, microbiology, molecular biology, mycology, nanoscience & nanotechnology, neurobiology, neuroscience, ornithology, paleobiology, paleontology, parasitology, pharmacology, physiology, plant biology, proteins & peptides, proteomics, radiation biology, reproductive biology, soil biology, structural biology, systems biology, techniques in biology, theoretical & mathematical biology, virology, zoology, and interdisciplinary applications in biology.
Articles
51 Documents
Evaluation of aquatic insects as bioindicators of water quality in industrial areas of Surabaya, East Java
Maximilliana Taek, Gemma Galgani
Genbinesia Journal of Biology Vol. 3 No. 3 (2024): July 2024
Publisher : Generasi Biologi Indonesia
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.55655/genbinesia.v3i3.58
In the industrial area of Surabaya, water quality monitoring is crucial for maintaining environmental sustainability and human health. Aquatic insects have been identified as effective bioindicators for assessing water quality in this context. This article aims to explore the biological characteristics of aquatic insects, their role as bioindicators, and their relationship with water quality parameters near the industrial area of Surabaya. Through a literature review approach, relevant sources were analyzed to obtain comprehensive insights. The biological characteristics of aquatic insects, such as abundance, species diversity, tolerance to pollution, and life cycle and reproduction, enable them to provide indications of pollution levels and the industrial impact on aquatic ecosystems. Pollution[1]sensitive aquatic insects, including certain species of Ephemeroptera, Plecoptera, and Trichoptera (EPT), tend to decline in abundance and diversity when water quality deteriorates. Conversely, the dominance of pollution-tolerant species, such as Chironomidae (non-biting midges), may indicate the impact of industrial pollution on water quality. Additionally, disruptions to the life cycle and reproduction of aquatic insects can signal water quality issues near Surabaya’s industrial zone. A decrease in reproduction or delayed growth in aquatic insects may indicate the presence of toxic substances or unsuitable environmental conditions. Water quality monitoring should not solely rely on aquatic insect bioindicators. Other parameters, such as pH, dissolved oxygen, turbidity, and chemical indicators, are essential for a more comprehensive analysis.
The effect of different light colors on the growth of Nannochloropsis sp.
Nurul Istiqomah;
Mukhlis, Alis;
Abidin, Zaenal
Genbinesia Journal of Biology Vol. 4 No. 1 (2024): November 2024
Publisher : Generasi Biologi Indonesia
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.55655/genbinesia.v4i1.59
The purpose of this study was to determine the effect of different light colours on population density, relative growth and self-replication time in Nannochloropsis sp. This study used a completely randomized design experiment involving 5 treatments and 4 replications. Treatment A uses red light, treatment B uses yellow light, treatment C uses green light, treatment D uses blue light, and treatment E uses white light. Density observations were made every 36 hours. The results of the study showed that differences in the colour of light had a significant effect on population density and relative growth of Nannochloropsis sp. but no significant effect on self-replication time.
Broodstock maintenance technique of koi fish (Cyprinus rubrofuscus, Lacepede 1803) at the Center Fisheries of Freshwater Aquaculture, Sukabumi, West Java
Nafila Junia Azadia;
Kismiyati, Kismiyati
Genbinesia Journal of Biology Vol. 4 No. 1 (2024): November 2024
Publisher : Generasi Biologi Indonesia
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.55655/genbinesia.v4i1.60
Koi fish (Cyprinus rubrofuscus) is an ornamental fish that has a beautiful body shape and color so it has high economic value. Good maintenance of koi fish parents will produce superior koi fish seeds. The aim of this research is to find out the techniques for raising parent koi fish from preparation to spawning and to find out the problems faced and how to handle them. The methods used in this research are active participation, observation and interviews. The parameters observed include fecundity, FR, HR, and water quality (temperature, pH, and DO). The data obtained from the research was then analyzed descriptively. Based on research that has been carried out, the highest fecundity, FR and HR data obtained during 2 consecutive spawning were 60,480 eggs, 95.8% and 100%. Spawning was carried out semi-artificially using the GnRH hormone with a ratio of the parent's body weight male and female 1:1. The average water quality results measured include a temperature of 23.8 ℃, pH 6.7, and DO 3.2 and are included in optimal conditions for rearing parent koi fish.
Preliminary Study on Morphology, Meristics, and Morphometric Variations of Javan Spitting Cobra Naja sputatrix Boie, 1827 and Sumatran Spitting Cobra Naja sumatrana Müller, 1890
Dafa, Maula Haqul;
Yudha, Donan Satria;
Sulistio, Adian Dwi
Genbinesia Journal of Biology Vol. 3 No. 1 (2023): November 2023
Publisher : Generasi Biologi Indonesia
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.55655/genbinesia.v3i1.61
Cobras are venomous snakes from Elapidae Family widespread in Asia and Africa, including Indonesia. There are two species of cobras in Indonesia, the one being is Javan spitting cobra (Naja sputatrix Boie, 1827) and other is Sumatran spitting cobra (Naja sumatrana Müller, 1890). In Indonesia, N. sputatrix distributions are in Java, Bali, and Nusa Tenggara, meanwhile N. sumatrana spread across Sumatra and Kalimantan. Wide distribution means that there are also potentially wide variations in morphology, meristic, and morphometric characters. This research was conducted to determine variations of Javan and Sumatran spitting cobra with morphology, meristic, and morphometric characterization. Total number of 16 samples (N. sputatrix = 8, N. sumatrana = 8) were examined and measured. Visual observation focused on coloration and hood characteristics. Meristic observation uses head, dorsal, ventral, and subcaudal scalation. Morphometric observation measures SVL, TL, Tail, and HW. We found that hood morphology varies greatly between N. sputatrix and N. sumatrana. N. sumatrana differs between Sumatran and Borneo population. Morphometric variation is being less prominent with morphometric description that matches with previous literatures and researches.
In silico research of anti-CHIKF phytoconstituent-based from Physalis peruviana leaves via molecular docking and dynamics analyses
Setiyowati, Putri Ayu Ika;
Mahbubillah, M. Ainul;
Aini, Nur Sofiatul;
Rachmawati, Yuanita
Genbinesia Journal of Biology Vol. 3 No. 1 (2023): November 2023
Publisher : Generasi Biologi Indonesia
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.55655/genbinesia.v3i1.62
Chikungunya fever (CHIKF) is an infectious disease that has similar symptoms with dengue fever (DF). Several drugs have been offered to treat both dengue (DENV) and chikungunya virus (CHIKV). Investigating anti-CHIKF potential from nearby plants is one strategy to produce potential drug to reduce CHIKF in endemic countries. Physalis peruviana is one the promising object to be new anti-CHIKV drug candidate. This study aimed to analyze the anti-CHIKV agents from leaf parts of P. peruviana. Ligand and protein samples were collected from multiple sources. The phytoconstituents were evaluated their drug-likeness properties throughout SwissADME webservers. Selected ligands then docked via PyRX and measured the output by binding affinity. Visualization of the best outputs was carried out using BIOVIA Discovery Studio. CABS-flex was carried out to screen the RMSF of molecular dynamics activity of the best complex. The result showed that 1,2-benzenecarboxylic acid had the lowest binding affinity following suramin as control with -5.1 and -11.1 kcal/mol after targeting E2 domain protein of CHIKV. This led to the conclusion that 1,2-benzenecarboxylic acid could be forecast as predictive anti-CHIKF therapeutic candidate. Additional in vitro and in vivo studies are needed to validate this outcome.
3,3-dimethyl-octane from Physalis peruviana as promising anti-DENV via ADMET prediction of pkCSM open webserver
Prastiyanto, Muhammad Evy;
Solekha, Rofiatun;
Rohmah, Laila Ainur;
Yuanita Rachmawati;
Aini, Nur Sofiatul
Genbinesia Journal of Biology Vol. 3 No. 1 (2023): November 2023
Publisher : Generasi Biologi Indonesia
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.55655/genbinesia.v3i1.63
Dengue is caused by the dengue virus (DENV) and being prevalent in 100 tropical and subtropical countries including Indonesia. This disease is spread by Aedes mosquitoes. There is currently no clinically authorized medicine to treat the dengue fever. Physalis peruviana has ethnomedicine application and noted for its antioxidant activities. This study purpose to investigate the pharmacokinetics or ADMET of anti-DENV from leaf parts of P. peruviana. The phytoconstituents data were gathered from multiple sources. The drug property and ADMET prediction were assessed using pkCSM. Following online screening, 3,3-dimethyl-octane functioned as predictive anti-DENV therapeutic candidate. Further dry and wet lab studies are needed to validate this finding.
The Molecular and developmental insights into gonadogenesis and primordial germ cell migration in pacific white shrimp (Litopenaeus vannamei): A comprehensive review
Hananya, Ariel;
Iman, Mohamad Nurul;
Rifany, Ade;
Kusuma, Satria Aji;
Kurnia, Methodius Digna;
Widodo, Rubiyanto;
Efendy, Hendi Yanto;
Jacinda, Adinda Kinasih;
Akiyama, Dean Mikio;
Laiman, Hendri
Genbinesia Journal of Biology Vol. 3 No. 3 (2024): July 2024
Publisher : Generasi Biologi Indonesia
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.55655/genbinesia.v3i3.64
The Pacific white shrimp, Litopenaeus vannamei, is a key species in global aquaculture, valued for its rapid growth and high market demand. Understanding the molecular mechanisms of gonadogenesis and primordial germ cell (PGC) migration in this species is vital for optimizing breeding programs. This review consolidates current knowledge on these processes, highlighting the role of the vasa gene as a germ cell marker. Vasa expression was tracked from fertilized eggs to juveniles aged four months, revealing the conserved DEAD-box helicase motif essential for germ cell development. Early detection of vasa messenger ribonucleic acid (mRNA) in developmental stages such as nauplius and zoea indicates its role in germ cell specification. By the post-larval stages, PGCs formed clusters associated with somatic gonadal precursor cells (SGPs), which support PGC migration and development. Quantitative polymerase chain reaction (qPCR) and reverse transcription PCR (RT-PCR) analyses demonstrated dynamic changes in vasa gene expression, suggesting critical windows for PGC proliferation and differentiation. Comparative studies with other species, such as Macrobrachium rosenbergii and Fenneropenaeus chinensis, emphasize the evolutionary conservation of germ cell development mechanisms. These insights are crucial for enhancing reproductive management and sustainability in shrimp farming, facilitating targeted interventions to improve breeding efficiency and hatchery success.
Relationship of observation depth and time against Perna viridis spawn in coastal waters of Waiheru Village, Inner Ambon Bay
Rettob, Mujahiddin Permata Roman;
Eddy, La;
Suriani, Sanita
Genbinesia Journal of Biology Vol. 3 No. 2 (2024): March 2024
Publisher : Generasi Biologi Indonesia
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.55655/genbinesia.v3i2.67
The adhesion of green mussel spit is highly correlated with depth. This study aims to establish the correlation between the depth and duration of observation with the abundance of green mussel spat (Perna viridis) in the coastal waters of Waiheru Village, located in Inner Ambon Bay. This study was undertaken utilising three floating net cages (KJA) possessed by local fishermen. The KJA utilised in this investigation had dimensions of 2.0 x 2.5 metres. Each KJA is equipped with 4 threads of hanger rope, resulting in a total of 12 hangers. Each rope hanger is positioned at specific depths, specifically at depths of 3, 6, and 9 metres. The net is 40 cm by 40 cm. Observations were conducted on days 2, 4, 6, 8, 10, 12, and 14 after the nets were strung. The findings indicated that the depth and duration of observation significantly influenced the quantity of green mussel spat. Specifically, more spat was observed compared to other days, suggesting a stronger attachment at that depth and observation period. Nevertheless, on the 10th day of observation, there was a surge in the quantity of green mussel spit.
Medicinal importance of Cassia alata L. (Fabaceae): A comprehensive review
Turista, Dora Dayu Rahma;
Lathifah, Qurrotu A’yunin;
Puspitasari, Eka;
Murtadlo, Ahmad Affan Ali;
Aini, Nur Sofiatul
Genbinesia Journal of Biology Vol. 3 No. 2 (2024): March 2024
Publisher : Generasi Biologi Indonesia
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.55655/genbinesia.v3i2.68
Cassia alata included in the Fabaceae family which spread in tropical and humid areas. All part of the C. alata contains phytochemical so that C. alata is potential medicinal plants. Leaves extract was reported to produce flavonoids, alkaloid, tannin, and cynogenic glycoside. Stems extract contains alkaloid, flavonoid, saponins, oxalate, pheno, and tannin. Roots extract contains alkaloid, saponins, flavonoids, tannins, and phenols. Flower contains saponin, tannin, anthraquinones, flavonoid, glicoside, steroid, and volatile oil. C. alata has been reported to be antiinflamatory, antioxidant, antibacterial, antifungal, antiviral, antiparasitic, antitumor, anticancer, antidiabetic, hepatoprotective, and have a laxative effect. The aim of this study is to give a sneak peek view on C. alata’s taxonomy, distribution, phytochemical, pharmacological activities, and the toxicological effects.
The The potential of meat boiling water waste on plants: A systematic literature review
Erviana, Syeni;
Fikri, Achmad Ali
Genbinesia Journal of Biology Vol. 4 No. 1 (2024): November 2024
Publisher : Generasi Biologi Indonesia
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
DOI: 10.55655/genbinesia.v4i1.69
Boiling water waste from meat is often considered as useless waste, whereas it can be utilized as a potential source to support plant growth. The aim of this research is to identify the potential of meat boiling water waste as a nutrient source for plants, as well as to evaluate its impact on plant growth and soil quality. This study was conducted using a systematic literature review method, which involved the collection and analysis of various previous studies relevant to the researched topic. Through this literature review, it was found that meat boiling water contains essential nutrients such as nitrogen, phosphorus, potassium, and micronutrients that can enhance soil fertility and support plant growth. The research results indicate that the use of meat boiling water can contribute to plant growth, although there are potential negative impacts such as microbial contamination, nutrient excess or fat deposition if not managed properly. This research is expected to serve as an alternative organic fertilizer that can support sustainable agriculture, provided that it is managed correctly and considers risk factors. These findings provide new insights into waste management in the agricultural sector, with the hope of reducing dependence on chemical fertilizers and providing environmentally friendly solutions. In conclusion, meat boiling water waste has the potential to be a useful nutrient source for plants; however, careful management is required to minimize its negative impacts. Further research is needed to optimize the use of this waste in sustainable agriculture.