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bionature
ISSN : 14114720     EISSN : 26545160     DOI : 10.35580
BIONATURE adalah jurnal yang berisi tulisan yang diangkat dari hasil penelitian, gagasan konseptual, kajian dan aplikasi teori di bidang biologi. Diterbitkan dua kali setahun pada bulan april dan oktober oleh Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Makasar
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Articles 296 Documents
Uji Hepatoterapi Ekstrak Etanol Buah Merah (Pandanus conoideus Lam.) Asal Kabupaten Jayawijaya Papua dengan Parameter SGPT terhadap Tikus Putih Jantan Aulia, Wati; Hendra, Herman; Dewi Purnama, Sari
bionature Vol 14, No 2 (2013): Oktober
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (90.026 KB) | DOI: 10.35580/bionature.v14i2.1454

Abstract

Penelitian ini bertujuan untuk meneliti efek hepatoterapi ekstrak etanol buah merah  dengan parameter SGPT.  Hewan uji dibagi menjadi 5 kelompok, yaitu kelompok kontrol negatif, kelompok kontrol positif, kelompok ekstrak 2% b/v, 4% b/v dan 6% b/v yang diinduksi menggunakan parasetamol dan diberi terapi selama 7 hari.  Pengukuran kadar SGPT dilakukan pada hari pertama sebelum induksi, setelah induksi dan terapi.  Data yang diperoleh dianalisis statistik menggunakan rancangan acak kelompok  dan  uji lanjutan Duncan menunjukkan bahwa terdapat perbedaan yang sangat nyata antara kelompok kontrol dengan kelompok perlakuan  pada taraf 1% dan pemberian suspensi ekstrak etanol buah merah 2%, 4%, 6% b/v memberikan efek penurunan kadar SGPT. Dari hasil penelitian dapat disimpulkan bahwa ekstrak etanol buah merah mempunyai efek hepatoterapi dan  pemberian suspensi ekstrak buah merah 6% b/v tidak berbeda nyata dengan penurunan kadar SGPT darah pada pemberian suspensi curliv 2500 mg/kgbb.
Penerapan Strategi Pemecahan Masalah Wankat & Oreovocz terhadap Hasil Belajar Siswa dalam Mata Pelajaran Biologi Kelas X SMAN 1 Sungguminasa Kabupaten Gowa. Humardani, Ryan
bionature Vol 17, No 1 (2016): April
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (443.552 KB) | DOI: 10.35580/bionature.v17i1.2590

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Penelitian ini bertujuan untuk mengetahui adanya perbedaan antara hasil belajar biologi siswa kelas X SMA Negeri 1 Sungguminasa  yang menggunakan strategi pemecahan masalah Wankat dan Oreovocz dengan siswa yang tanpa penerapan strategi pemecahan masalah Wankat dan Oreovocz. penelitian ini adalah penelitian eksperimen semu (quasy experimental) dengan desain penelitian Posttest-Only Control Design. Populasi adalah seluruh Siswa Kelas X SMA yang berjumlah 303 orang dan sampel sebanyak 82 orang. Instrumen penelitian yang digunakan berupa tes hasil belajar Biologi dan angket yang dianalisis menggunakan statistik deskriptif dan analisis statistik inferensial. Berdasarkan data hasil penelitian, skor hasil belajar biologi Siswa yang mengikuti pelajaran dengan penerapan strategi pemecahan masalah Wankat & Oreovocz mempunyai rata-rata 86 sedangkan skor hasil belajar biologi Siswa yang mengikuti pelajaran tanpa penerapan model strategi pemecahan masalah Wankat & Oreovocz  mempunyai rata-rata 58. Hasil analisis statistik inferensial (uji-t) diperoleh thitung 10 lebih besar dari ttabel 2,021 sehingga Ho ditolak dan  H1 diterima. Jadi penerapan strategi pemecahan masalah Wankat & Oreovocz dapat meningkatkan hasil belajar Biologi siswa kelas X SMA Negeri 1 Sungguminasa Kab.Gowa.Kata kunci: Hasil Belajar Biologi, Strategi Pemecahan Masalah Wankat dan Oreovocz
Pembentukan Kapsul/Nodul Larva Helicoverpa armigera Hubner (Lepidoptera : Noctuidae) Pasca Infeksi HaNPV (Helicoverpa armigera NUCLEAR POLYHEDROSIS VIRUS) Nani, Kurnia
bionature Vol 11, No 1 (2010): April
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Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.088 KB) | DOI: 10.35580/bionature.v11i1.1375

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NPV is one of the biological insecticides that kills the target by infecting their cells including hemocytes. Hemocytes play a key role on defence mechanism against pathogens like NPV. This defense mechanism may cause the insect to become more tolerant as well as to chemical insectisides. The tolerances of insect to NPV could be induced by cellular immune system (CIS). One of the CIS is capsules/nodules formation. Based on that assumption, this research was conducted to observe the alteration of capsules/nodules formation of Helicoverpa armigera larvae post sublethal infection of HaNPV (Helicoverpa armigera nuclear polyhedrosis viruses). The fifth instar Helicoverpa armigera ware infected by sublethal doses of HaNPV. 24 hour post infection, partly of survive larvae reared to get their first (NPV-F1) and second filial (NPV-F2). Another survivor induced by hit killed Bacillus cereus, and then its haemocoel were exposed under stereo microscope (total magnification, 60 X) to watch capsules/nodules that appear as black spots on haemolymph. This procedure also conducted to fifth instar larvae of NPV-F1, NPV-F2, control (K-P) and their filial (K-F1 and K-F2), blank and placebo. The result showed that the number of nodules/capsules on larvae were not significant between control and the NPV-treat larvae on every generation, which was about 4 nodules/capsules. It showed that there is no cellular immune respon changed on NPV-P larvae, so their immune ability are similar to larvae K-P. The recoveries of NPV-P larvae cause it have a good capability to produce offspring with good immune response. So, NPV-F1 and NPV-F2 larvae have same ability as K-F1 and K-F2 larvae. It can be concluded that the use of sublethal dosage of HaNPV has not changed formation of capsules/nodules of H. armigera larvae and their offspring.
Identifikasi dan Uji Kemampuan Hidrolisis pada Bakteri Amilolitik dan Proteolitik yang Diisolasi dari Wadi, Makanan Khas Kalimantan Tengah Choirunnisa, Hesti Nur; Sari, Ria Yustika; Hastuti, Utami Sri; Witjoro, Agung Witjoro
bionature Vol 18, No 2 (2017): Oktober
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Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (433.895 KB) | DOI: 10.35580/bionature.v18i2.6138

Abstract

Abstract. Wadi is a sort of traditional foods that made of fish from Dayak tribe in Central Kalimantan. Wadi is made by the addition of salt and lumu. People make wadi as a supply of side dishes during difficult seasons of fish and stock for farming, hunting, or collecting forest products. In wadi, there are several bacteria species that capable in degrading amylum and protein. The bacteria capability in amylum and protein degradation can be determined by calculating the hydrolysis index. The aims of this study are to: (1) identify the amylolytic bacteria and proteolytic bacteria species from wadi, (2) determine the amylum and protein hydrolysis index on each amylolytic bacteria and proteolytic bacteria species isolated from wadi, and (3) determine the amylolytic bacteria and proteolytic bacteria species that have the highest hydrolysis index. This research is descriptive explorative research. The research results showed that: (1) there were four isolates of amylolytic bacteria isolated from wadi, i.e: Enterobacter agglomerans, Pseudomonas fluorescens, Acinetobacter baumannii, and Micrococcus varians, and there were five species of isolated proteolytic bacteria from wadi, i.e: Enterobacter agglomerans, Pseudomonas fluorescens, Nitrococcus mobilis, Acinetobacter baumannii, and Micrococcus varians, (2) the amylum hydrolysis index of Enterobacter agglomerans is 3.77; on Pseudomonas fluorescens is 3.00; on Acinetobacter baumannii is 4.16; and on Micrococcus varians is 7.23. The protein hydrolysis index of Enterobacter agglomerans is 3.22; on Pseudomonas fluorescens is 2.25; on Nitrococcus mobilis is 1.67; on Acinetobacter baumannii is 3.42; and on Micrococcus varians is 3.45, (3) Micrococcus varians has the highest amylum, i.e:  7.23 and protein hydrolysis index is 3.45.Keywords: hydrolysis index, amylolytic bacteria, proteolytic bacteria, wadi.
Pengaruh Cara Pengolahan Umbi Tire (Amorphophallus sp.) terhadap Kadar Kalsium Oksalat Syamsiah, .
bionature Vol 12, No 2 (2011): Oktober
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Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (482.765 KB) | DOI: 10.35580/bionature.v12i2.1398

Abstract

The influence of processing method of tire tuber (Amorphophallus sp.) toward the level of calcium oxalate at Faculty of Mathematics and Natural Sciences, State University of Makassar was to finding out the effect of tuber processing methods tire against calcium oxalate and know the best way of processing. This research conducted in experiment central laboratory of the Ministry of Health, Indonesia, and Makassar. This research consisted of the dependent variable in the form of calcium oxalate in the tuber tire and independent variables such as processing methods. Samples were tire tuber (Amorphophallus sp.) processed by boiling it (A1), salt-water immersion (A2), and wood ash (A3). The research data obtained using titrimetry. Data were analyzed using inferential analysis technique and LSD test at 0.05 significance level. Average-content of calcium oxalate of tire tuber weighing one gram for A0, A1, A2, and A3 respectively was 0.056%, 0.008%, 0.034%, and 0.040%. From the data, it appears there was the influence of processing methods, both those treated with boiling water immersion salt and wood ashes, on serum calcium oxalate in the tuber tire. The most effective way of processing was to reduce or minimize calcium oxalate in the tuber tire processing by boiling. 
Analisis Nilai pH dan Tingkat Kerusakan Silase Pakan Komplit yang Diformulasi Dengan Daun Lamtoro (Leucaena leucocephala) Sebagai Pakan Ternak Ruminasia Qadarullah, M Nurul; Munir, Munir; Irmayani, Irmayani
bionature Vol 19, No 2 (2018): Oktober
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Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (327.703 KB) | DOI: 10.35580/bionature.v19i2.9729

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Abstract. The purpose of this study was to determine the effect of adding lamtoro leaves (leucaenanleucocephale) on pH values and the level of damage at different levels. This study used a completely randomized design (CRD) with 4 treatment levels and 3 replications, namely J0= lamtoro leaf 0%, J1= addition of lamtoro leaves 40%, J2=addition of lamtoro leaves 30%, J3=addition of lamtoroleaves 20%. The results showed that the addition of lamtoro leaves to feed silage has a very real effect. on average the highest to lowest pH value is J0 is (5.16), J1 is (3.83), J2 is (4.67), and J3 is (4.73). Average damage to the highest to lewest is J0 is (6.67), J1 is (1.76), J2 is (4.38), and J3 is (4.78). From the results of the study, it is known that the best treatment and recommended defects are J1 treatment with the addition of lamtoro leaves 40%. Keywords: pH, damage rate, complete feed silage, lamtoro leaf, and rice strawid
Upaya meningkatkan disiplin guru dalam kehadiran mengajar di kelas melalui keteladanan kepala sekolah di SMPNegeri 5 sengkang Kabupaten Wajo Altar, Hanatidah
bionature Vol 15, No 1 (2014): April
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Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (57.892 KB) | DOI: 10.35580/bionature.v15i1.1543

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Penelitian ini adalah Penelitian Tindakan Sekolah (Action Research) yang bertujuan untuk mengetahui peningkatan disiplin Guru dalam kehadiran mengajar di kelas melalui keteladanan kepala sekolah di SMP Negeri 5 Sengkang Kabupaten Wajo. Subyek penelitian ini adalah 13 orang tenaga pendidik (guru) SMP Negeri 5 Sengkang. Penelitian dilaksanakan pada semester ganjil tahun pelajaran 2012/2013. Hasil penelitian menunjukkan bahwa keteladanan kepala sekolah dapat meningkatkan kedisiplinan guru dalam kehadiran mengajar di kelas, dimana pada pelaksanaan pembelajaran pra-siklus pada pertemuan pertama semua Guru hadir tepat waktu, karena merupakan hari pertama sekolah untuk tahun pelajaran 2012/2013. Pelaksanaan pembelajaran pada siklus I ada 10 orang Guru yang terlambat masuk mengajar di kelas, dan setelah pelaksanaan pembelajaran pada siklus II kedisiplinan guru dalam kehadiran mengajar di kelas mencapai 80%  berdasarkan tanggapan responden (Guru) mengenai keteladanan kepala sekolah. Berdasarkan hasil observasi 77%  Guru yang masih terlambat kurang dari 5 menit, karena lokasi penelitian memang agak sulit terjangkau.
Inventarisasi Jenis Pohon Inang Anggrek Epifit di Desa Tompobulu Resort Balocci Taman Nasional Bantimurung Bulusaraung Kabupaten Pangkep Provinsi Sulawesi Selatan Sumarni, Sumarni; Hiola, St. Fatmah; Karim, Hilda
bionature Vol 20, No 2 (2019): Oktober
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Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (464 KB) | DOI: 10.35580/bionature.v20i2.11279

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Abstract. Balocci Resort Banntimurung Bulusaraung National Park is a location that is overgrown with many natural orchids. This research is a descriptive study that aims to determine the type of epiphytic orchid host tree and the distribution of host trees at the Balocci Resort in Bantimurung Bulusearaung National Park. The method used is a survey method. The stages of the research are termining location and observations, collecting data and indetifying spesies. Based on the result; Alstonia  scholaris, Sauarauia malayana, Mangifera sp, Tabernaemontana sphaerocarpa, Arenga pinnata, Syzygium sp, Elaecarpus angustifolius, Bischofia javanica, Maesea sp, Erythrina subumbrans, Lithocarpus celebicus, Cryptocarya sp, Elmerillia ovalis, Memecylon paniculatum, Ficus benjamina L, Ficus variegate, Ficus fistulosa, Knema laurina, Litsea sp, Uncaria longiflora, Coffea sp, Melicope sp, Planchonella firma, Palaquium lobbianum, and Laportea stimulant which belongs to 19 families. The tree that is overgrows with orchids is Alstonia  scholaris, Sauarauia malayana and Ficus benjamina L. the host tree which is used as a place for growth has uneven surface characteristics of the tree, overgrown with nails and generally mossy. Host tree species that have a wide spread on mount Bulusaraung is Sauarauia malayana which is at an altitude of 700-1300 m above sea level. Keywords: host tree, epiphytic, resort balocci, bantimurung bulusaraung national park area
Kajian Pembentukan Gum Xanthan dari Limbah Padat Sagu oleh Xanthomonas campetris Palennari, Muhiddin; Rante, Herlina
bionature Vol 10, No 1 (2009)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v10i1.264

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The purpose of these research was studying of capability for Xanthomonas campetris to bioconversion toward sago solid waste became xanthan gum, to know pH influences and sugar consentration for forming of high xanthan gum. Based on the research was done revealed that X. campetris which doing bioconversion process become xanthan gum which achieved rendemen about 11,026%. Optimum pH to producing of xanthan gum was obtained 7.0. the consentration of sugar for forming the high xanthan gum which 4%. The result of thesereseach is expected to give  benefit in supporting seeking of the cheap substrate for xanthan gum producing. Keywords : xanthan gum, sago solid waste, Xanthomonas campetris
Peningkatan Pembentukan Embrio Somatik pada Wortel (Daucus carota L) Menggunakan N6-benzylaminopurine (BAP) ., Rusdianto; Indrianto, A.
bionature Vol 16, No 2 (2015): Oktober
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (375.982 KB) | DOI: 10.35580/bionature.v16i2.2462

Abstract

Salah satu proses pembentukan planlet dalam teknik kultur jaringan tumbuhan adalah embriogenesis somatik, yaitu suatu proses pembentukan embrio dari eksplan yang berupa sel-sel somatik yang telah mengalami dediferensiasi. Penelitian ini bertujuan meningkatkan pembentukan embrio somatik pada waortel (Daucus carota L) menggunakan BAP. Penelitian ini dilakukan dalam 3 tahap yaitu; (1) Tahap perkecambahan  in-vitro, menggunakan medium ¼ MS; (2) Tahap induksi dan pemeliharaan kalus, menggunakan medium MS + 2,4-D 2 mg/l ; (3) Tahap induksi embrio somatik. Hasil penelitian menunjukkan biji wortel dapat berkecambah dengan baik pada medium ¼ MS dengan rata-rata persentase perkecambahan mencapai 98 % dan panjang hipokotil 3,84 cm. Efisiensi pembentukan kalus mencapai 90.83 %, Warna kalus umumnya putih bening atau putih kekuningan dengan tekstur friable atau remah. Perlakuan 0,5 mg/l BAP merupakan konsentrasi yang paling tepat meningkatkan pembentukan embrio somatik, dengan rata-rata jumlah embrio fase globuler 54,00; fase jantung 5,33; dan fase torpedo 4,66, dibandingkan dengan konsentrasi perlakuan BAP yang lain.

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