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Majalah Kedokteran Bandung
Published by Universitas Padjadjaran
ISSN : 0126074X     EISSN : 23386223     DOI : 10.15395/mkb
Core Subject : Health,
Medicine & Pharmacology
Majalah Kedokteran Bandung (MKB)/Bandung Medical Journal publishes peer-reviewed original articles and case reports in basic medical research, clinical research, and applied medical science. This journal is published quarterly (March, June, September, and December) by Faculty of Medicine Universitas Padjadjaran. Articles are original research that needs to be disseminated and written in English. All submitted manuscripts will go through the double-blind peer review and editorial review before being granted with acceptance for publication.
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Articles 651 Documents
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Peningkatan Kadar Hemoglobin dengan Pemberian Ekstrak Daun Salam (Syzygium Polyanthum (Wight) Walp) pada Tikus Model Anemia Defisiensi Besi Adyani, Kartika; Anwar, Anita D.; Rohmawaty, Enny
Majalah Kedokteran Bandung Vol 50, No 3 (2018)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (26.792 KB) | DOI: 10.15395/mkb.v50n3.1390

Abstract

Defisiensi zat besi merupakan penyebab utama anemia di negara berkembang. Kadar hemoglobin darah digunakan sebagai penanda anemia defisiensi besi. Indonesia kaya bahan makanan tinggi zat besi namun belum diketahui kemanfaatannya seperti daun salam (Syzygium polyanthum (Wight) Walp). Tujuan penelitian ialah mengetahui peningkatan kadar hemoglobin pada tikus model anemia defisiensi besi sesudah pemberian ekstrak daun salam. Penelitian eksperimental laboratorium dengan rancang acak lengkap dilakukan di Laboratorium Farmakologi dan Terapi Fakultas Kedokteran Unpad periode 4 Mei–19 Juni 2018. Sebanyak 20 ekor tikus Wistar betina berumur 7 minggu diinduksi menggunakan alumunium sulfat 67,5 mg/kg BB secara intramuskuler selama 7 hari, dan dibagi menjadi 5 kelompok perlakuan, yaitu K1 (tanpa perlakuan) K2 (tablet tambah darah 5,4 mg), P1 (ekstrak daun salam 2,2 mg), P2 (ekstrak daun salam 4,4 mg), P3 (ekstrak daun salam 6,6 mg). Kadar hemoglobin diukur menggunakan auto hematology analyzer sysmex. Uji LSD menunjukkan rerata kadar hemoglobin setelah perlakuan pada kelompok perlakuan berbeda signifikan dibanding dengan kelompok kontrol negatif (p<0,05) dan tidak berbeda signifikan dibanding dengan kelompok kontrol positif (p>0,05). Analisis uji median menunjukkan peningkatan kadar hemoglobin berbeda signifikan pada K2, P1, P2, dan P3 (p<0,05). Disimpulkan, ekstrak daun salam meningkatkan kadar hemoglobin pada tikus model anemia defisiensi besi. Increasing Hemoglobin Level Using Bay Leaf (Syzygium Polyanthum (Wight) Walp) Extraction in Rats Models with Iron-Deficiency AnemiaIron deficiency is a major cause of anemia in developing countries. Blood hemoglobin level is used as a marker of iron-deficiency anemia. Iron-rich foods that are not widely known can easily be found in Indonesia, including bay leaf. The aim of this study was to determine the increase in hemoglobin level after the administration of bay leaf extract in rat models with iron-deficiency anemia. This experimental study used completely randomized sampling technique and was performed at Pharmacology and Therapy Laboratory, Faculty of Medicine, Universitas Padjadjaran, from 4 May to 19 June 2018. Twenty 7-week-old female Wistar rats were induced intramuscularly by 67,5 mg/kg BW alumunum sulfate for 7 days and were divided into 5 groups: K1 (without treatment), K2 (5.4 mg of ferrous sulphate), P1 (2.2 mg bayleaf extract), P2 (4.4 mg  bay leaf extract), P3 (6.6 mg bay leaf extract). Hemoglobin levels were measured using Auto Hematology Analyzer Sysmex. Results froom LSD test showed that the average hemoglobin levels after treatment in treatment groups were significantly different from that of the negative control group (p<0.05) and there was no  significant difference when compared to the positive control group (p>0.05). The median analysis test showed that there were significant differences in the increase of hemoglobin levels among K2, P1, P2, and P3 (p<0.05). It is concluded that bay leaf simplicia increases the hemoglobin level in rat model with iron-deficiency anemia.
KADAR PROTEIN S-100 SERUM PENDERITA FRAKTUR OROMAKSILOFASIAL YANG DISERTAI CEDERA KEPALA RINGAN Miguna, Hayana; Arifin, Muhammad Zafrullah
Majalah Kedokteran Bandung Vol 44, No 4 (2012)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Penderita fraktur oromaksilofasial sering disertai dengan cedera kepala karena letak dan strukturnya yang berdekatan. Penilaian kemungkinan untuk lesi intrakranial setelah cedera kepala ringan merupakan tantangan utama dalam mendiagnosisnya. Salah satu metode yang dapat digunakan yaitu dengan menggunakan biomarker (petanda biokimia) protein S-100 serum yang merupakan protein neuron-spesifik. Tujuan penelitian adalah untuk menilai peningkatan kadar protein S-100 serum dan melihat perbedaannya berdasarkan lokasi fraktur oromaksilofasial pada cedera kepala ringan. Penelitian ini menggunakan metode studi kasus kontrol yang dilakukan di RS Dr. Hasan Sadikin Bandung pada bulan November 2010?Januari 2011. Sampel berjumlah 76 orang yang terdiri atas 38 penderita dewasa fraktur oromaksilofasial disertai dengan cedera kepala ringan dan 38 orang dewasa sehat sebagai kontrol. Hasil penelitian menunjukkan bahwa pada penderita fraktur oromaksilofasial dengan cedera kepala ringan diperoleh kadar protein S-100 serum rata-rata meningkat sebanyak dua kali lipat, yang secara statistik signifikan dengan t hitung=2,26 atau nilai p=0,0135. Bila kelompok penderita yang satu dibandingkan dengan kelompok penderita lainnya, maka didapatkan hasil yang tidak signifikan secara statistik atau dapat dikatakan mempunyai kadar protein S-100 serum yang sama. Simpulan, pada penderita dewasa fraktur oromaksilofasial disertai cedera kepala ringan terdapat peningkatan kadar protein S-100 serum dan tidak terdapat perbedaan kadar protein S-100 serum berdasarkan lokasi fraktur oromaksilofasial. [MKB. 2012;44(4):233?39].Kata kunci: Cedera kepala ringan, fraktur oromaksilofasial, protein S-100 serumSerum Levels of S-100 Protein in Oromaxillofacial Fracture Patients with Mild Head InjuryOromaxillofacial fracture is often accompanied by head injury due to its adjacent location and structure. Risk estimation for clinically relevant intracranial lesions after minor head injury remains a major diagnostic challenge. One possible method to evaluate the possibility of intracranial lesion is by using biomarkers (biochemical marker) protein S-100. Protein S-100 is a neuron-specific protein. The aim of present study was to assess the elevated levels of S-100 protein serum and to investigate whether there was any difference in the S-100 protein serum concentrations depending on the location of the oromaxillofacial fractures in mild head injury. This study was performed using analytic observational method with case control research design conducted at Dr. Hasan Sadikin General Hospital Bandung between November 2010 and January 2011. There were 76 samples, comprised of 38 adult patients with oromaxillofacial and mild head injury and 38 healthy adults as a control group. The result showed that there was an increase level of S-100 protein serum concentrations in patients having oral and maxillofacial fractures with mild head injury. The concentration increased on average twice as much, which was statistically significant at t test=2.26 with p-value=0.0135. If one group of patients was compared with other patient groups, however, the results were not statistically significant, or it can be stated to have the same level of S-100 protein serum concentrations. In conclusion, oral and maxillofacial fractures in adult patients with mild head injury have elevated levels of S-100 protein serum concentrations and there is no difference in S-100 protein serum concentrations based on the location of oromaxillofacial fractures in mild head injury. [MKB. 2012;44(4):233?39].Key words: Mild head injury, oromaxillofacial fracture, S-100 protein serum DOI: http://dx.doi.org/10.15395/mkb.v44n4.178
Ekspresi CD3 dan CD26 pada Limfosit T sebagai Biomarker Potensial Penyakit Systemic Lupus Erythematosus Suselo, Yuliana Heri; Balgis, Balgis; Indarto, Dono
Majalah Kedokteran Bandung Vol 48, No 3 (2016)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1266.598 KB) | DOI: 10.15395/mkb.v48n3.843

Abstract

Systemic lupus erythematosus (SLE) merupakan penyakit autoimun yang sering dijumpai pada wanita. Penyakit ini ditandai oleh hiperautoreaktivitas limfosit T dan B. Di dalam sistem imun, CD3 dibantu CD26 sebagai molekul kostimulator berkaitan erat dengan aktivasi dan migrasi limfosit T. Pada penyakit SLE, ekspresi CD3 dan CD26 serta aktivitas enzim CD26 belum diketahui. Tujuan penelitian ini adalah mengetahui ekspresi CD3 dan CD26 dalam darah serta kultur limfosit T pasien SLE. Rancangan penelitian ini bersifat eksperimen laboratorium dengan pendekatan studi retrospektif. Penelitian dilakukan di Laboratorium Biomedik, Fakultas Kedokteran Universitas Sebelas Maret Surakarta selama lima bulan (Mei–September 2012). Diagnosis SLE ditentukan menurut kriteria dari American College of Rheumatology (ACR). Darah vena diambil dari tiga pasien SLE dan dua orang sehat. Satu µg/mL phytohaemmaglutinin (PHA) digunakan untuk stimulasi kultur limfosit T. Ekspresi CD3 dan CD26 ditentukan dengan flows sytometry. Substrat H-Gly-Pro pNA digunakan untuk menguji aktivitas enzim CD26. Data yang terkumpul dianalisis dengan uji t. Ekspresi CD3 dan CD26 menurun dalam darah dan kultur limfosit T pada pasien SLE dibanding dengan kontrol, sedangkan aktivitas enzim CD26 pada kultur limfosit T pasien SLE lebih tinggi daripada kontrol (0.042 vs 0.030 U/mL), tetapi perbedaan tersebut tidak bermakna secara statistik (p>0.05). Simpulan, terdapat penurunan ekspresi CD3 dan CD26 baik disirkulasi darah maupun di kultur limfosit T subtipe CD4+. CD3 dan CD26 berpotensi sebagai biomarker penting untuk SLE. Namun, riset lanjutan masih perlu dilakukan untuk menjelaskan peran keduanya dalam patogenesis penyakit SLE. [MKB. 2016;48(3):140–7]Kata kunci: CD3, CD26, systemic lupus erythematosus (SLE)CD3 and CD26 Expression on T Lymphocytes as a Potential Biomarker of Systemic Lupus ErithematosusSystemic lupus erythematosus (SLE) is an autoimmune disease that is frequently found in women and characterized by hyperautoreactivity of T and B cells. In the immune system, expressions of CD 3 and CD26 (as co-stimulatory molecule) are related to T cells activation and migration. Co-expression of CD3 and CD26 in SLE patients has not been determined. The aim of this study was to investigate the co-expression of CD3 and CD26 in blood and T cell culture of SLE patients. This was an analytical descriptive study with a retrospective approach. This study was performed at the Biomedical laboratory, Faculty of Medicine, Sebelas Maret University, for five months (May–September 2012). SLE diagnosis was determined by using the criteria from the American College of Rheumatology (ACR). Vein blood was collected from three female patients with SLE and two healthy female controls. T cells isolated from the blood were cultured and stimulated with 1 µg/mL phytohaemmaglutinin (PHA). Flow cytometry was used to determine the coexpression of CD3 and CD26. CD26 enzyme activities in T cell culture were spectrophotometrically measured using H-Gly-Pro pNA substrate. Collected data were then analyzed using Student’s t test. Decreased coexpression of CD3 and CD26 was lower in blood samples and T cell cultures of SLE female patients than in control. Meanwhile, CD26 enzyme activities in SLE T cell cultures were higher than control (0.042 vs 0.030 U/mL) but no statistical difference was found (p>0.05). In conclusion, there is a decreased coexpression of CD3 and CD26 in blood circulation and T cell cultures subtype CD4+. CD3 and CD26 in SLE patients could be a prospective biomarker. Further research is required to unravel the roles of CD3 and CD26 in SLE pathogenesis. [MKB. 2016;48(3):140–7]Key words: CD3, CD26, systemic lupus erythematosus (SLE) 
Comparison of Enzyme-Immunoassay and Rapid Immunochromatography Test for Detecting Helicobacter pylori Stool Antigen Prasetyo, Dwi; H. J, Gerritsen; P., Mertens; V., Labrune; T, Leclipteux; E. J, Kuijper
Majalah Kedokteran Bandung Vol 46, No 1 (2014)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

The rapid immunochromatography for Helicobacter pylori stool antigen (HpSA) test is a recently developed method for detection of Heicobacter pylori (H. pylori)  infection. The objective of this study was to compare rapid HpSA test with a conventional enzyme-immunoassay (EIA) and to determine the prevalence of H. pylori infection in symptomatic (recurrent abdominal pain/RAP) Indonesian children. One hundred and two high school children aged 12–18 years old in Bandung with RAP from July to November 2008 were enrolled, excluding children who had additional symptoms, such as diarrhea or fever. First study was performed in 102 samples in laboratory of Leiden University Medical Center (LUMC), Helicobacter pylori infection was detected by a conventional EIA and rapid test. A second study was performed on a stored collection of 32 feces samples previously tested positive for H. pylori by EIA in laboratory of LUMC; these samples were retested by both assays. The results showed the sensitivity, specificity, positive predictive value, and negative predictive value of the rapid test were 100%, 98.2%, accuracy 98.5%, 92.6% and 100%, respectively, from 134 tested samples. The overall prevalence of H. pylori infection among Indonesian children was 3% by EIA at first testing. In conclusion, the prevalence of H. pylori infection among symptomatic Indonesian children is very low and the new developed Pylori-Strip test shows good performance, very fast, and easy to use compared to EIA.  [MKB. 2014;46(1):52–6]Key words: Children, diagnostic, Helicobacter pylori, immunochromatographyPerbandingan Pemeriksaan Enzyme-Immunoassay dan Rapid Immunchromatography untuk Mendeteksi Helicobacter pylori Stool AntigenPemeriksaan Helicobacter pylori stool antigen (HpSA) menggunakan rapid immunochromatography (rapid HpSA) merupakan metode yang saat ini sedang berkembang untuk mendeteksi infeksi H. pylori. Tujuan penelitian ini untuk membandingkan metode rapid HpSA dengan metode konvensional yaitu enzyme-immunoassay (EIA) dan menentukan prevalensi infeksi H. pylori pada anak dengan gejala sakit perut berulang (SPB) di Indonesia. Sebanyak 102 anak sekolah menengah usia 12–18 tahun di Bandung dengan keluhan SPB ikut dalam penelitian ini, dilaksanakan dari Juli–November 2008. Anak dengan gejala tambahan, seperti diare dan demam, dikeluarkan dari penelitian. Penelitian pertama dilakukan pada sampel feses 102 anak di laboratorium Leiden University Medical Center (LUMC), Helicobacter pylori terdeteksi baik dengan pemeriksaan EIA konvensional maupun rapid test. Penelitian kedua dilakukan pada 32 buah sampel feses simpanan di laboratorium LUMC yang sebelumnya menunjukkan hasil positif dengan pemeriksaan EIA, sampel ini juga diperiksa menggunakan kedua metode. Hasil penelitian menunjukkan sensitifitas, spesifisitas, akurasi, positive predictive value, dan negative predictive value, dengan hasil 100%, 98,2%, 98,5%, 92,6%, dan 100% dari 134 sampel yang diuji. Prevalensi infeksi H. pylori pada anak Indonesia dengan keluhan SPB sekitar 3% pada pemeriksaan pertama. Simpulan, prevalensi infeksi H. pylori pada anak SPB di Indonesia sangat sedikit dan pemeriksaan Pylori-strip (rapid test) merupakan pemeriksaan yang sangat baik dan cepat serta mudah digunakan dibandingkan dengan EIA. [MKB. 2014;46(1):52–6]Kata kunci: Anak, diagnosis, Helicobacter pylori, immunochromatographyDOI: 10.15395/mkb.v46n1.228
Jumlah Sel Piramidal CA3 Hipokampus Tikus Putih Jantan pada Berbagai Model Stres Kerja Kronik Arjadi, Fitrianto; Soejono, Sri Kadarsih; Maurits, Lientje Setyawati; Pangestu, Mulyoto
Majalah Kedokteran Bandung Vol 46, No 4 (2014)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Paparan stres kronik dan berkepanjangan mengakibatkan hilangnya neuron di regio  CA3 (cornu ammonis) hipokampus dan  penurunan kognitif. Tujuan penelitian  mengetahui perbedaan jumlah sel piramidal CA3 hipokampus tikus putih jantan pada model stres kerja meliputi   paradoxical sleep deprivation (PSD), imobilisasi, dan footshock kronik. Metode penelitian adalah  post-test only with control group design experimental dengan rancangan acak lengkap  menggunakan 24 ekor tikus putih jantan galur Wistar usia 3–4 bulan yang dibagi 4 kelompok:  KI kontrol tanpa perlakuan,  KII (stres PSD), KIII (stres imobilisasi), dan KIV (stres footshock) dan sel piramidal CA3 hipokampus  diwarnai dengan toluidine-blue. Jumlah sel dihitung menggunakan  perangkat lunak Image raster v2.1, perbesaran 400x tiap 10 lapangan pandang. Penelitian dilakukan  6 bulan (April–September 2012) di Laboratorium Hewan Coba, Fakultas Kedokteran dan Ilmu Kesehatan Universitas Jenderal Soedirman. Analisis data menggunakan analysis of variance (ANOVA) dengan Post-Hoc least significant difference (LSD) menunjukkan  perbedaan jumlah sel piramidal CA3 hipokampus signifikan (p=0,037) pada minimal dua kelompok perlakuan. Uji statistik dengan Post-Hoc LSD menunjukkan perbedaan signifikan jumlah sel piramidal CA3 hipokampus antara  kelompok kontrol (12,9±2,47) dan kelompok imobilisasi (9,00±1,53) (p<0,05). Simpulan, kelompok imobilisasi kronik memiliki jumlah sel piramidal CA3 hipokampus terendah dibandingkan dengan ketiga kelompok lainnya.Kata kunci:  Model stres kerja kronik, sel piramidal CA3 hipokampus, tikus putih jantanNumber of CA3 Pyramidal Cell in Male Albino Rat   Hippocampus Exposed to Various Chronic Work Stress Models AbstractProlonged and chronic exposure to stress leads to the loss of neurons at the CA3 (cornu ammonis) hippocampus region and spatial memory deficits. The aim of this study was to study the number of CA3 pyramidal cells in albino rats that were exposed to chronic stress of works model consisting of paradoxical sleep deprivation (PSD), immobilization, and foot shock stresses. The method applied was the post-test only method with control group experimental design using completed randomized design (CRD on 24 3–4 month old male Wistar rats . The rats were divided into 4 groups: group I (control), group II (PSD stress), group III (immobilization stress), and group IV (footshock stress). The CA3 pyramidal cell hippocampus was stained with toluidine-blue. The number of CA3 pyramidal cell of hippocampus was counted using Image raster v2.1 software at 400x magnification in 10 duplicates for each sample. The study was conducted in six months (April–September 2012) at the Animal Laboratory, Faculty of Medical and Health Sciences, Jenderal Soedirman University. Analysis for the differences in the number of CA3 pyramidal cells was conducted using analysis of variance (ANOVA) with Post-Hoc LSD. The results of the ANOVA  showed a p value=0.037, meaning that there was significant difference in at least two groupsof treatment. Further statistical test using Post-Hoc LSD showed a significant difference  between the control group (12.9 ± 2.47) and the chronic immobillization group (9,00 ± 1,53) (p<0.05). In conclusion, the chronic immobillization stress group has the lowest average number of hippocampus CA3 pyramidal cells compared to other groups Key words: CA3 pyramidal cell in hippocampus, chronic works stress model, male albino rats DOI: 10.15395/mkb.v46n4.337
Gambaran Validitas Pemeriksaan Complex Specific Cocktail Antigen Mycobacterium tuberculosis (ESAT-6, CFP-10, MPT-64) Metode Rapid Immunochromatography pada Bahan Pemeriksaan Sputum dan Serum Penderita Tuberkulosis Paru Subroto, Hendra; Parwati, Ida; Turbawaty, Dewi Kartika; Alisjahbana, Bachti
Majalah Kedokteran Bandung Vol 49, No 3 (2017)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.856 KB) | DOI: 10.15395/mkb.v49n3.1120

Abstract

Penegakan diagnosis tuberkulosis (TB) paru penting dalam mengurangi morbiditas dan mortalitas. Diagnosis laboratorium TB paru berdasar atas pemeriksaan BTA dan kultur M. tuberculosis memiliki sensitivitas rendah. Terdapat pemeriksaan cocktail antigen TB rapid immunochromatography (ICT) yang mendeteksi antigen ESAT-6, CFP-10, MPT-64 yang disekresikan oleh M. tuberculosis. Tujuan penelitian menganalisis validitas pemeriksaan cocktail antigen TB metode rapid ICT sputum dan serum penderita TB paru terhadap kultur Ogawa. Penelitian dilaksanakan Juli–Oktober 2014 di RSUP Dr. Hasan Sadikin Bandung. Bentuk penelitian adalah observasional deskriptif khusus dengan rancangan penelitian potong lintang. Subjek penelitian penderita yang datang ke Poliklinik Pulmonologi atau Poliklinik DOTS, didiagnosis TB paru. Sebanyak 68 sputum dan serum dari 33 kultur sputum M. tuberculosis positif dan 35 kultur negatif dilakukan pemeriksaan cocktail antigen TB rapid ICT. Angka positivitas cocktail antigen TB rapid ICT sputum 54,4%; serum tanpa pemanasan 0%. Pada serum dilakukan pemanasan pada suhu 56oC selama 30 menit untuk menghilangkan aktivitas antibodi dan didapatkan angka positivitas sebesar 19,1%. Nilai sensitivitas dan spesifisitas pemeriksaan untuk sputum 93,9% dan 82,8%, untuk serum tanpa pemanasan 0% dan 100%, serta serum dengan pemanasan 24,2% dan 85,7%.  Validitas pemeriksaan sputum memiliki sensitivitas tinggi dan spesifisitas sedang, untuk serum memiliki sensitivitas rendah dan spesifisitas tinggi. [MKB. 2017;49(3):178–85]Kata kunci: Cocktail antigen TB rapid ICT, kultur Ogawa, mikroskopik BTA, tuberkulosis paru, serum, sputum Validity of Complex Specific Cocktail Antigen Mycobacterium tuberculosis (ESAT-6, CFP-10, MPT-64) Rapid Immunochromatography Method on Sputum and Serum Samples from Patient with Pulmonary TuberculosisEarly diagnosis of pulmonary tuberculosis (TB) is very important in reducing morbidity and mortality. The current diagnosis of TB includes direct staining (acid fast bacilli) or M. tuberculosis culture, but these examinations have a low sensitivity. An assay using rapid ICT cocktail antigen TB is currently available for diagnosing TB. This method can detect ESAT-6, CFP-10, and MPT-64 antigen which is secreted by M. tuberculosis. The aim of this study was to analyze the validity of cocktail antigen TB rapid ICT using sputum and serum with Ogawa culture. This was a cross-sectional descriptive observational study. Sputum and serum were collected from patients who were diagnosed as lung TB suspects in the lung and DOTS Clinic of Dr. Hasan Sadikin General Hospital Bandung during the period of July–December 2014 in . Cocktail antigen TB detection assay using two kind of samples (sputum and serum) were evaluated. A total of 68 subjects of33 subjects presented positive culture and 35 presented negative cultures. Positivity rates for sputum and serum were 54.4% and 0%, respectively. Heated sputum assay had a sensitivity of 93.9% and specificity of 82.8%, Serum assay presented a sensitivity of 0% and specificity  of100%.  Serum were modified by heating at 56oC for 30 minutes. The positivity rate of heated serum was 19.1%. The result of modified serum assay showed a sensitivity  of 24.2% and specificity of 85.7%. Conclusion: the sensitivity of the sputum assay is high and the specificity is medium. The sensitivity of this serum assay is low and the specificity is high. [MKB. 2017;49(3):178–85]Key words: Acid fast bacilli, cocktail antigen TB rapid ICT, pulmonary tuberculosis, Ogawa culture, sputum, serum
DIFERENSIASI ASMA ATOPIK DENGAN NONATOPIK PADA PASIEN RAWAT JALAN DI KLINIK PARU-ASMA Santoso, Prayudi; Dahlan, Zul
Majalah Kedokteran Bandung Vol 45, No 2 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Terdapat kesulitan penatalaksanaan asma karena mekanisme asma yang kurang jelas dan terdapat berbagai fenotipe asma yang bersifat individual memerlukan terapi pendekatan individual. Salah satu fenotipe asma adalah asma atopik dan nonatopik yang secara klinis susah dibedakan. Penelitian bersifat deskriptif analitik, rancangan potong lintang dilakukan untuk melihat perbedaan karakteristik asma atopik dengan nonatopik. Subjek penelitian penderita asma baru yang berobat jalan di klinik Paru Asma An-Nur Bandung tahun 2007?2008. Pada pasien tersebut dilakukan pemeriksaan nilai total IgE, spirometri, dan tes tusuk kulit. Terdapat 198 pasien asma terdiri atas 149 (75,3%) pasien atopik dan 49 (24,7%) nonatopik. Median usia pasien nonatopik adalah 39 tahun dan atopik 34 tahun (p=0,039). Terdapat perbedaan bermakna keluhan sesak napas kelompok atopik (68,5%) dan nonatopik (44,9%) (p=0,003), tidak bermakna pada pemeriksaan spirometri. Riwayat atopik pasien sendiri terdapat pada kelompok asma atopik (p=0,011), bermakna pada rinitis (81,2 vs 61,2 OR 2,7) dan urtikaria (14,8 vs 4,1 OR 4,0). Asma nonatopik tidak lebih berat dibandingkan dengan atopik yang diketahui dari pemeriksaan persentase forced expiratory volume first second (FEV1). Pada pasien rinitis dengan urtikaria, risiko mendapatkan asma atopik lebih besar. Simpulan, tidak ada perbedaan hasil tes tusuk kulit pada kedua kelompok. Gejala klinis kelompok atopik sesuai dengan positivitas hasil pemeriksaan IgE. Keadaan klinis rinitis dan urtikaria lebih menjuruskan diagnosis ke arah asma atopik dibandingkan dengan gambaran klinis lainnya atau hasil spirometri. [MKB. 2013;45(2):105?11]Kata kunci: Asma atopik, asma nonatopik, IgE, karakteristik klinisDifferentiation of Atopic and Non-Atopic Asthma in Out-Patient Pulmo-Asthma ClinicThere is a difficulty in asthma management regarding to unclear mechanism of asthma, and a variety of asthma phenotypes that individually require individual therapy and approach. The asthmatic phenotype atopic and nonatopic was clinically difficult to distinguish. The study was analytical descriptive with cross-sectional design. The study aim was to explore the differences of atopic and non-atopic asthmatic characteristics. The study subjects were newly asthmatic patients who were out-patients of An-Nur Lung-Asthmatic Clinic, Bandung, in 2007?2008. In patients examined total IgE, spirometry and skin prick test. There were 198 asthmatic patients comprising 149 (75.3%) atopic and 40 (24.7%) non-atopic asthma. The median of the non-atopic age was 39 years, while the atopic was 34 years (p=0.039). There was a significant difference between atopic (68.5%) and non-atopic (44.9%) in complaint of short breath (p=0.003), but non significant in spirometric examination. The history of atopy were present in atopic asthma group (p=0.011), with significance in rhinitis (81.2 vs 61.2 OR=2.7) and urticaria (14.8 vs 4.1 OR=4.0). Non-atopic asthma was not more severe that atopic asthma was known from examination of forced expiratory volume first second (FEV1). Rhinitis patient, having urticaria, have greater risk for atopic asthma. There were no difference in skin prick test results between both groups. The clinical symptoms of atopic group were in line with the positivenes results of IgE examination. Clinical condition of rhinitis and urticaria direct to the diagnosed towards atopic asthma compared than other clinical features or spirometric result. [MKB. 2013;45(2):105?11]Key words: Atopic asthma, clinical characteristics, IgE, non-atopic asthma DOI: http://dx.doi.org/10.15395/mkb.v45n2.112
Aktivitas Polifenol Teh Hijau (Camellia sinensis (L) O. Kuntze) Sebagai Imunomodulator melalui Respons Supresi Imunoglobulin E (IgE) pada Rinitis Alergika Yusni, -; Husni T. R,, Teuku; Achmad, Tri Hanggono
Majalah Kedokteran Bandung Vol 47, No 3 (2015)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Rinitis alergika adalah kondisi inflamasi mukosa nasal yang diakibatkan oleh interaksi antara alergen dan imunoglobulin E (IgE). Imunomodulator merupakan bagian terpenting dalam pengobatan rinitis alergika dan salah satu tanaman obat yang mempunyai aktivitas imunomodulator adalah teh hijau (Camellia sinensis L.) terutama polifenol. Tujuan penelitian adalah menganalisis aktivitas polifenol teh hijau sebagai imunomodulator pada pasien rinitis alergika. Penelitian ini dilaksanakan dari bulan Juni–Desember 2011 di laboratorium Fisiologi Fakultas Kedokteran Unsyiah Banda Aceh dan pemeriksaan IgE dilakukan di laboratorium swasta. Desain penelitian adalah quasi experimental dengan rancangan pretest-posttest with control group. Subjek penelitian adalah 12 pasien rinitis alergika, yaitu 6 orang sebagai kelompok kontrol (diberikan plasebo) dan 6 orang sebagai kelompok perlakuan (diberikan kapsul polifenol teh hijau 2x350 mg/hari, selama 14 hari). Analisis data menggunakan uji normalitas, uji homogenitas, dan uji-t (p<0,05). Hasil penelitian menunjukkan kadar imunoglobulin E sesudah pemberian perlakuan pada kelompok perlakuan lebih rendah dibanding dengan kelompok kontrol (1.475,2±940,7 vs 494,3±366,5 IU), namun tidak bermakna (p=0,05). Simpulan pemberian polifenol teh hijau  menurunkan sekresi IgE, namun tidak bermakna dan membutuhkan penelitian lebih lanjut.  [MKB. 2015;47(3):160–66]Kata kunci: Imunoglobulin E, imunomodulator, polifenol teh hijau (Camellia sinensis L.), rinitis alergika Activity of Green Tea (Camellia sinensis (L) O. Kuntze) Polyphenols as Immunomodulator through Response of Suppression Immunoglobulin E (IgE) in Allergic RhinitisAbstractAllergic rhinitis is an inflammatory condition of the nasal mucosa caused by  interactions between allergens and immunoglobulin E (IgE). Immunomodulatory is an important part of the treatment of allergic rhinitis. One of the medicinal plants that have immunomodulatory activities is green tea (Camellia sinensis L.), specifically polyphenols. The purpose of this study was to analyze the activity of green tea’s polyphenols as an immunomodulator in patients with allergic rhinitis. This study was conducted in  June to December 2011 in the laboratory of Physiology, Faculty of Medicine Unsyiah with the IgE examinations conducted in private laboratories. This study is a pretest-postest quasi experimental study with control group design. Subjects were 12 patients with allergic rhinitis;  6 people were included in the control group (placebo) and 6 in the treatment group (green tea’s polyphenols 2x350 mg/day, for 14 days). Analysis of the data was performed using the  normality test, homogeneity test, and t-test (p<0.05). The results showed that the levels of immunoglobulin E after the administration of green tea’s polyphenols in the treatment group was lower than the control group (1.475.2±940.7 vs 494.3±366.5 IU), but not significantly (p=0.05). In conclusion, the administration of green tea’s polyphenols can insignificantly decrease the secretion of IgE. Hence, further research is required. [MKB. 2015;47(3):160–66]Key words: Allergic rhinitis, immunoglobulin E,  immunomodulator, green tea’s polyphenols (Camellia sinensis L.)DOI: 10.15395/mkb.v47n3.596
Perbandingan R-Baux Score dengan BOBI Score sebagai Prediktor Mortalitas Pasien Luka Bakar di RSUP Dr. M. Djamil Padang Fitri, Azdiana; Saputra, Deddy; Putra, Andani Eka
Majalah Kedokteran Bandung Vol 50, No 2 (2018)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v50n2.1326

Abstract

Sistem skoring pada luka bakar sangat dibutuhkan sebagai nilai objektif untuk prediktif mortalitas. Belum ada sistem skoring objektif terbaik sebagai prediktif mortalitas luka bakar. Penelitian ini bertujuan membandingkan akurasi R- Bauxdan BOBI score dalam memprediksi mortalitas pasien luka bakar di RSUP Dr. M. Djamil, Padang. Penelitian dilakukan Januari 2013 sampai September 2017, menggunakan desain studi retrospektif pada pasien luka bakar yang dirawat di Unit Luka Bakar RSUP Dr. M. Djamil, Padang. Data diolah menggunakan uji diagnostik, uji Kappa, dan uji regresi logistik terhadap variabelnya. Jumlah sampel adalah 394 sampel. Sampel terbanyak laki-laki, rerata usia 28,3 ± 18,3 tahun, penyebab luka bakar terbanyak api, rerata total body surface area (TBSA) 26,1%, trauma inhalasi 51% dan angka mortalitas 26,4%. Uji sensitivitas BOBI score dibanding dengan R-Baux score adalah 96,83%: 92,89% dengan koefisien Kappa sebesar 0,50. Analisis regresi logistik menunjukkan variabel umur, TBSA, dan trauma inhalasi saling berhubungan pada R-Baux score. Uji diagnostik BOBI score lebih baik dibanding dengan R-Bauxscore dan nilai koefisien Kappa menunjukkan kesesuaian hasil dengan BOBI score. BOBI score lebih baik sebagai prediktor mortalitas di RSUP Dr. M. Djamil Padang dibanding dengan R-Bauxscore karena menunjukkan akurasi lebih baik setelah diuji dengan nilai real. Kata kunci: BOBI score, luka bakar, mortalitas, r-baux scoreComparison between R-Baux Score and BOBI Score as a Predictor of Burn Mortality in Dr. M. Djamil Hospital PadangBurns are the type of trauma with high morbidity and mortality. No best  objective scoring system is currently available to predict mortality in burn cases. This study aimed to compare the accuracy of R-Baux and BOBI scores in predicting mortality among burn patients in Dr. M. Djamil Hospital, Padang. This was a retrospective study on burn patients treated in burn unit of this hospital from January 2013 to September 2017. Assessments were performed on diagnostic test, kappa tests, and logistic regression test. Out of 394 samples enrolled men were more prominent, while the mean age of these patients was 28.3±18.3 years old with fire as the most frequent cause. The, mean TBSA and inhalation injury were  26.1% and 51%, respectively, with an overall mortality of 26.4%. The sensitivity test performed to compare  BOBI and R-Baux scores revealed the result of 96.83%: 92.89% with o.50 coefficient’s value of Kappa. Logistic regression test showed that age, TBSA, dan inhalation injury significantly correlated with R-Baux score, Diagnostic test result of BOBI score was better than R-Baux score and the coefficient’s value of Kappa showed a matched result with BOBI score with medium strength. BOBI score shows better accuracy as the mortality predictor of burn cases in Dr. M. Djamil Hospital Padang.Key words: BOBI score, burns, mortality, r-baux score
PERAN EKSTRAK ETANOL TOPIKAL DAUN MENGKUDU (MORINDA CITRIFOLIA L.) PADA PENYEMBUHAN LUKA DITINJAU DARI IMUNOEKSPRESI CD34 DAN KOLAGEN PADA TIKUS GALUR WISTAR Rahmayani S., Indah Puti; Maskoen, Ani Melani; Hernowo, Bethy S.
Majalah Kedokteran Bandung Vol 45, No 4 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Luka akan menimbulkan masalah jika penanganannya kurang baik sehingga menyebabkan luka kronik. Mengkudu (Morinda citrifolia L.) merupakan tanaman khas daerah tropis termasuk Indonesia, yang buah, daun, dan akarnya sering digunakan dalam pengobatan tradisional, di antaranya untuk menyembuhkan luka. Penelitian dengan rancangan eksperimental sederhana ini bertujuan untuk mengetahui efek penggunaan ekstrak etanol daun mengkudu terhadap penyembuhan luka pada pemberian topikal, dengan melihat gambaran histopatologis yang terdiri atas sel fibroblas, infiltrasi sel inflamasi, imunoekspresi cluster of differentiation 34 (CD34), dan deposisi kolagen. Penelitian dilakukan di laboratorium Farmakologi dan Patologi Anatomi RS Dr. Hasan Sadikin Bandung pada November 2010?September 2011. Penelitian ini dilakukan pada 36 tikus dengan membuat eksisi pada punggung tikus. Kemudian tikus-tikus tersebut dibagi menjadi 2 kelompok yaitu kelompok kontrol dan perlakuan ekstrak etanol daun mengkudu topikal pada luka. Jaringan luka tiap kelompok diamati secara mikroskopis pada hari ke-3, 7, dan 14. Hasil penelitian menunjukkan penyembuhan luka kelompok perlakuan cenderung lebih baik, dilihat pada data hari ke-3, 7, dan 14 pada semua parameter. Uji statistik Mann-Whitney pada tingkat kepercayaan 95% (p<0,05) untuk jumlah fibroblas, infiltrasi sel inflamasi, imunoekspresi CD34, dan kolagen berturut-turut p=0,319; p=0,290; p=0,251; dan p=0,245. Simpulan, ekstrak etanol topikal daun mengkudu cenderung bermanfaat pada penyembuhan luka, walaupun secara statistik tidak bermakna. [MKB. 2013;45(4):226?33]Kata kunci: CD34, daun mengkudu, kolagen, penyembuhan lukaRole of Noni (Morinda citrifolia L.) Leaf Ethanolic Extract TopicalApplication on Wound Healing Examined from CD34 Immunoexpression and Collagen on Wistar RatsProblems in wound healing occurred if proper care is not given and the wound develops into a chronic wound. Noni (Morinda citrifolia L.) is one of the most common plants in tropical areas, including Indonesia, which fruit, leaves dan root are used in traditional treatment, for example wound healing. This experimental research with post test-only control group design identified the effect of topical application of noni leaves ethanol extract on wound healing by examining the histopathological appearance of fibroblas count, inflammatory cell infiltration, cluster of differentiation 34 (CD34) immunoexpression, and collagen deposition. The research was performed between November 2010 until September 2011 at the Pharmacology and Pathology Anatomy laboratories of Dr. Hasan Sadikin General Hospital Bandung. Excisions were made on each back of the rat of the 36 rats that were divided into control and test groups where the test group received topical application of noni leaves ethanol extract. The wound was examined on day 3, 7, and 14 using a light microscope. The result showed a tendency of better wound healing in the test group for all parameters based on the data on day 3, 7, and 14. Mann-Whitney Test with 95% confidence interval (p<0.05) showed that the p value for fibroblast count, inflammation cell infiltration, CD34 immunoexpression and collagen subsequently were p=0.319, p=0.290, p=0.251, and p=0.245, respectively. In conclusion, topical application of noni leaves ethanol extract has a benefit on wound healing although the results are not statistically significant. [MKB. 2013;45(4):226?33]Key words: CD34, collagen, noni leaves, wound healing DOI: http://dx.doi.org/10.15395/mkb.v45n4.169

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