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BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Published by Southeast Asian Regional Centre for Tropical Biology
ISSN : 02156334     EISSN : 1907770X     DOI : http://dx.doi.org/10.11598/btb
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary
BIOTROPIA, The Southeast Asian Journal of Tropical Biology, is a scientific publication of the Southeast Asian Ministers of Education Organization (SEAMEO) – Southeast Asian Regional Center for Tropical Biology (BIOTROP). It publishes articles in the broad areas of tropical ecosystems and environmental impacts, biodiversity conservation and sustainable development and biotechnology.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 520 Documents
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Identification of Endophytic Bacteria From Curcuma zedoaria Based on Protein Profile Using Maldi-Tof Mass Spectrometry Sulistiyani, Tri Ratna; Lisdiyanti, Puspita
BIOTROPIA Vol. 25 No. 2 (2018): BIOTROPIA Vol. 25 No. 2 August 2018
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2018.25.2.795

Abstract

Valid identification of microorganisms is vital information to establish culture collections. Currently, a molecular approach based on 16S rDNA is widely used for bacterial identification. However, this approach is time-consuming and expensive. Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) allows the identification of microorganisms directly from colonies, and it only takes a few minutes. The aim of this study was to identify endophytic bacteria associated with Curcuma zedoaria based on protein profiles using the MALDI-TOF MS system and to compare the results with 16S rDNA sequencing. Endophytic bacteria were isolated from parts of the medicinal plant C. zedoaria collected from Bogor, West Java, Indonesia. The identification of selected bacteria was performed using protein profiling via MALDI-TOF MS. A total of 66 endophytic bacteria from C. zedoaria were selected for identification. The results of MALDI-TOF MS analysis showed that eleven isolates (16.67%) were correctly identified at the species level, and twenty-three isolates (34.85%) matched the genus level of the molecular approach. These results demonstrate that the MALDI-TOF system is a suitable and feasible approach for bacterial identification, particularly for screening and grouping large numbers of isolates.
BROWN SPOT CAUSED BY CURVULARIA SPP., A NEW DISEASE OF ASPARAGUS B. SALLEH, B. SALLEH; SAFINAT, A.; JULIA, L.; TEO, C.H.
BIOTROPIA No. 9 (1996)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.1996.0.9.123

Abstract

The distribution, aetiology and symptomatology of a new disease on asparagus ferns, which we have termed brown spot, is described. Descriptions of and a key to identification of the causal organisms, Curvularia brachyspora, C. eragrostidis, C. lunata and C. pallescens, are also presented. Pathogenicity tests showed that C. lunata was the dominant and most virulent of the four species. Inoculation with conidial suspensions or mycelial transfers through wounded ferns were more effective in inducing the disease than inoculations on unwounded ferns. This is the first record of C. brachyspora in Malaysia and the first report of this disease on asparagus Key words: Malaysia/Plant diseases/Brown spotJCurvularia brachyspora/Curvularia eragrostidts/Curvularialunata/Curvularia pallescens/Asparagus.
The Liverwort Genus Marchantia (Marchantiaceae) of Mount Sibayak North Sumatra, Indonesia Siregar, Etti Sartina; Ariyanti, Nunik S.; Tjitrosoedirdjo, Sri S.
BIOTROPIA Vol. 20 No. 2 (2013): BIOTROPIA Vol. 20 No. 2 December 2013
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2013.20.2.327

Abstract

Study on the Hepaticae (Liverworts) of Sumatra is very scanty including the knowledge on the genus of Marchantia (Marchantiaceae). This study was conducted to explore the diversity of Marchantia in Mount Sibayak North Sumatra, Indonesia. There are seven species of Marchantia in Mount Sibayak North Sumatra, five of the species are previously known: Marchantia acaulis, M. emarginata, M. geminata, M. paleacea, and M. treubii; two of the species are new records for Sumatra (M. polymorpha and M. rubribarba). An identification key of the genus Marchantia from Mount Sibayak, North Sumatra is provided.Key words: Marchantia, Mount sibayak, North Sumatra
Carbon Sequestration of Mangrove Ecosystem in Segara Anakan Lagoon, Indonesia Hilmi, Endang; Sari, Lilik Kartika; Cahyo, Tri Nur; Kusmana, Cecep; Suhendang, Endang
BIOTROPIA Vol. 26 No. 3 (2019): BIOTROPIA Vol. 26 No. 3 December 2019
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2019.26.3.1099

Abstract

Carbon conservation programs in mangrove ecosystems focus on the growth of mangrove vegetation that is measured based on the amount of carbon present at different tree stages, particularly the seedlings, saplings, and mature mangrove trees. This study was aimed to determine carbon percentage of mangrove ecosystems using the SNI 06 — 3730 — 1995 and TAPPI T 211 om 85 methods, and to analyse the mangrove clustering based on carbon percentage. The results showed that (1) Avicennia spp., Sonneratia spp., Bruguiera spp., Rhizophora spp., Aegiceras spp., Lumnitzera spp., Ceriops spp., Excoecaria agallocha and Xylocarpus granatum had carbon percentages between 45.01% and 55.54%; (2) the carbon percentage of mangroves at different growth stages were as follows; seedlings at 16.3-21.2%, saplings at 19.0-28.1%, trees with diameter at breast height (DBH) of 10-20 cm at 38.1-46.30%, trees with DBH of 20-30 cm at 40.2-51.1% and trees with DBH of 30-40 cm at 49.1-55.20%. The carbon conservation is positively correlated with the carbon sequestration ability and growth of the mangroves.
Litter Fall in A Primary and Two Logged-over Lowland Tropical Rainforests in Pasirmayang, Jambi. WASRIN, UPIK ROSALINA; PUTERA, AGUS EKA
BIOTROPIA No. 14 (1999)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.1999.0.14.156

Abstract

Litter accumulation in a  primary and a logged-over  lowland dipterocarp  forest at Pasirmayang, Jambi was measured using the litter trap method. In the primary forest, traps were placed in four distinct areas, reflecting the succession stages of the forest from building to maturation. In the logged-over forest, litter production was measured at two different sites, one cut in 1979/1980 and the second in 1983/1984. In the primary forest, average litter production during the observation period was 925 g m"2yr'. In the logged-over forest, average litter production was 721 g m'2 yr1 for the site cut in 1979/1980 and 706 g m'2 yr1 for the site cut in 1983/1984. Leaves comprised the major contributor of litter with 67% of total litter produced in the primary forest, 67% of total litter in the 1979/1980 cut logged-over forest, and 65% of total litter in the 1983/1984 cut logged-over forest. The purpose of the study was  to use litter fall as a measure of forest productivity  to assess the recovery of logged-over forests and, to provide a basis for comparison of forest-derived land practices for appropriate forest management strategies. Key words: Litter production/primary forest/logged-over forest/forest productivity/Pasirmayang/Jambi.
The Expansion of Merremia peltata (L.) Merrill in Fragmented Forest of Bukit Barisan Selatan National Park Enhanced by Its Ecophysiological Attributes Yansen, Yansen; Wiryono, Wiryono; Deselina, Deselina; Hidayat, Muhammad F.; Depari, Efratenta K.
BIOTROPIA Vol. 22 No. 1 (2015): BIOTROPIA Vol. 22 No. 1 June 2015
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2015.22.1.353

Abstract

Forest opening and fragmentation may affect the composition of vegetation by permitting the expansion or domination of one or two species in the area. This study found that , an invasive climbing creeping Merremia peltata/species, has dominated some area of Bukit Barisan Selatan National Park (BBSNP). Domination of this species was favoured by forest fragmentation, indicated by the significantly higher number of individuals of in the open M. peltataarea than in intact forest. The ecophysiological characters of support the ability of this species to become a M. peltatastrong invader. As the expansion of this species may negatively affect the biodiversity and vegetation health, the application of appropriate ecological approaches to control the domination of in the conservation area is M. peltatanecessary.
cDNA ENCODING GROWTH HORMONE FROM HUMPBACK GROUPER (Cromileptes altivelis) Syaifudin, Mochamad; Alimuddin; Widyastuti, Utut; Sudrajat, Agus Oman; Sumantadinata, Komar; Aliah, Ratu Siti
BIOTROPIA Vol. 14 No. 2 (2007): BIOTROPIA Vol. 14 No. 2 December 2007
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2007.14.2.14

Abstract

Growth hormone (GH) that plays an important role in growth, reproduction, seawater adaptation, and immune function was isolated and sequenced from humpback grouper, Cromileptes altivelis. The cDNA was isolated from pituitary using RT-PCR. The 618 bp open reading frame encodes a 205 amino acid (aa) protein, which represents an 18 aa signal peptide followed by a 187 aa mature GH polypeptide. The fragment contained conserved domain of somatotropin–1, somatotropin–2, casein kinase II phosphorylation, protein kinase C phosphorylation, N–myristoylation and N–glycosilation. The similarity of deduced protein of humpback grouper GH was 65.0 - 89.5% with other fishes
INFLUENCE OF MEVALONIC ACID AND LINALOOL ON LIMONENE ACCUMULATION IN CALLUS TISSUES OF CITRUS GRANDIS OSBECK ZARINA ZAKARIA; NIK NORULAINI NIK AB RAHMAN; MOHD OMAR ADD KADIRS
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 20 (2003)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2003.0.20.183

Abstract

Effect on callus growth was studied for Citrus grandis  cultured with feeding of exogenous mevalonic acid (MVA) at concentrations of 0.04, 0.08, 0.38, 0.77, 1.15 and 1.54 mM. Similar effect with linalool ranging from 10 to 200 nl was studied under various incubation periods. The growth was proportional to the concentrations of precursors used meaning that higher precursors concentrations influenced more growth on C. grandis callus culture. Mevalonic acid and linalool showed quite similar precursor feeding effects on limonene accumulation of C. grandis callus cultures. It was revealed that limonene production was triggered with the introduction of MVA and linalool even at low concentration. Limonene accumulation was detected as early as week four and continued to increase at about 0.0030 and 0.0032 mg/g with MVA and linalool, respectively, after the seventh week incubation. In comparison to the unfed cultures, no limonene was detected from the callus up to eight weeks in incubation. Keywords :   C/rrusgrarafo/Limonene/Linalool/Mevalonic acid Abbreviations: 2,4-D: 2,4 — Dichlorophenoxyacetic acid, ABA: Abscisic acid, MVA : Mevalonic acid
Seed Germination of Avicennia marina (Forsk.) Vierh. By Pericarp Removal Treatment Purnobasuki, Hery; Utami, Edy Setiti Wida
BIOTROPIA Vol. 23 No. 2 (2016): BIOTROPIA Vol. 23 No. 2 December 2016
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2016.23.2.349

Abstract

Avicennia marina is one species used for building coastal ecosystem stabilization on the Surabaya shores because of its tolerance to high salinity levels and resistance to wave energy. Its existence is threatened by various factors, including land conversion, pollution, and the construction of inter-island bridges. The growth of A. marina is currently limited by seed dormancy. To increase propagation efficiency, seed viability and vigor were compared based on collection dates and pericarp removal methods. The results showed no relationship between the salinity levels of soaking water or seed buoyancy and seed viability or vigor. Collection dates may influence seed viability and vigor, as seeds collected in October and November germinated more quickly than those collected in December, although there were no differences in total germination. Hand-peeled seeds germinated more slowly than soaked seeds for all collection dates, but with no differences in total germination. Seeds collected in October and November grew more vigorously than those collected in December.
KARAMUNTING (Melastoma malabathricum) EXTRACTS ON WHITE SHRIMP (Litopenaeus vannamei) MATURITY Ahmad Ridwan; Awaludin Awaludin
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 28 No. 2 (2021): BIOTROPIA Vol.28 No.2, Agustus 2021
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.0.0.0.1297

Abstract

Lanosterol is a phytosterol that is used by crustacea to form animal steroid hormone that plays a role in the reproduction of the shrimp. This research aims to determine the response of ovary development in white shrimp parent after given Karamunting ethanol extract. This research was carried out in several stages: 1. Injecting the white shrimp every 3 days, at the base of the 5th leg, for 15 days with variable control dose 0 (C), 10 mg / kg BW (T1), 7.5 mg / kg BW (T2), 5 mg / kg BW (T3), 2 mg / kg BW (T4) and 1 mg / kg BW (T5), (2). Isolation of white shrimp parent ovary, (3). Measurement of progesterone level in the ovary using the Radioimmunoassay (RIA) method, (4) Histology of white shrimp parent ovary, and (5). Data analysis. The statistical results of measuring the increase in progesterone showed that administration of ethanol extract of karamunting at the end of maintenance had a significant difference (P˂0.05). Histology observations of gonadal development in control, T5 and T4 showed cells to develop to previtellogenesis oocytes whereas in treatment T1, T2 and T3 ovary cells developed into endogenous vitellogenesis oocytes and only in T1 ovarian cells developed to form exogenous vitellogenesis oocytes.  Statistical results of oocyte size after administration of the karamunting extract at the end of maintenance were significantly different (P˂0.05). The average oocyte size at the beginning of maintenance (15.57 ± 3.15 µm), C (25.29 ± 2.69 µm) indicating that at the end of the ovarian treatment development occurred both in control and treatment. T1 (65.65 ± 2.64 µm), T2 (63.98 ± 3.06 µm), T3 (39.12 ± 6.01 µm), T4 (28.08 ± 0.84 µm) and T5 (27.65 ± 0.71 µm) also appear to be greater than the oocyte size at the beginning of maintenance and control. Based on the results of this study it can be concluded that the content of lanosterol in karamunting plants can increase the hormone progesterone which indicates an acceleration of gonadal maturity and enlargement of oocyte size in the parent shrimp of White shrimp.

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