cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota surakarta,
Jawa tengah
INDONESIA
Nexus Biomedika
Published by Universitas Sebelas Maret
ISSN : -     EISSN : -     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 292 Documents
 21   22   23   24   25 
Diuresis Effect Comparison between Leaf Extract of Orthosiphon aristatus and Hydroclorotiazidto Male White Rat (Rattus novergicus) ANGGIE HERWANLISTANTO; Endang Ediningsih; Siti
Nexus Biomedika Vol 6, No 1 (2017): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.864 KB)

Abstract

Introduction: Kumis kucing leaf containing flavonol which can generate diuresis effect with increasing the excretion of natrium and chlorine. The purpose of this research was to know how did the diuresis effect of kumis kucing leaf extrack compared to hydroclorotiazid. Methods: The research was a laboratory experimental analytic study with posttest only control group design. The research subject was a number of 30 male white rats of wistar strain, aged about 2-3 months old and 150-200 grams of weight, which divided into five treatment groups. Group 1was given 2 ml CMC 1% as negative control, group 2 was given 0.32 mg hydroclorotiazid in 2 ml aquadest as positive control, group 3 was given kumis kucing leaf extract dose I (50 mg in 2 ml CMC 1%), group 4 was given kumis kucing leaf extract dose II (100 mg in 2 ml CMC 1%), group 5 was given kumis kucing leaf extract dose III (200 mg in 2 ml CMC 1%). Rats urine volume was measured every 6 hours for 24 hours observation. Result:The average measurements of rat urine volume for 24 hours showedthat group negative control had average volume3.3871.435 ml, the average volume of positive control was7.3070.607 ml,the average volume group dose I was 2.9000.350 ml, the averagevolume group dose II was 3.4900.311 mlanddose III had average volume 5.4330.641 ml. Result of anova statistical calculation showed there are significant difference urine volume in those five groups (p <0.05). Conclution:The research conclude that kumis kucing leaf extract had diuresis effect lower than hydroclorotiazid therapeutical dose. Keyword: kumis kucing leaf extract, hydroclorotiazid, diuresis
Deteksi Gen P16 pada Sampel Blok Parafin Lidah Penderita Karsinoma Sel Skuamosa Rongga Mulut di RSUD Dr. Moewardi. Coraega Gena Ernestine; Pradipto Subiyantoro; Vita Nirmala
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Oral squamous cell carcinoma is a part of the cancer in the head and neck which ranks 10th most cancers in the world with a wide geographical distribution. It is usually found in the late stage. P16 gene (CDKN2a/INK4a) is an important tumor suppressor gene in carcinogenesis. This study aimed to detect the expression of p16 gene on paraffin block samples of the tongue of the oral squamous cell carcinoma patients in RSUD Dr. Moewardi. Methods: DNA product used in this study was isolated from 14 samples on Paraffin Blocks of the tongue of the oral squamous cell carcinoma patients in RSUD Dr. Moewardi. The DNA products, then, were used as a template for PCR amplification of the p16 gene. Primers were designed with the base sequences deposited in Gene Bank. PCR amplification products were run in agarose gel electrophoresis and visualized to detect the presence of the p16 gene. Later, the results were analyzed and presented in descriptive data. Results: Only 6 of 14 samples were diagnosed as oral squamous cell carcinoma. From 6 samples only one was detected positive expression of p16 gene. DNA bands were detected in 3% agarose gel. The intensity of DNA bands was visualized lowly and there was a smear. On the 5 other samples, DNA bands were detected under the marker, thus they showed a negative result or the expression of p16 gene was not detectable. Conclusions: Among 6 samples of the tongue of the oral squamous cell carcinoma patients, only one showed positive expression of p16 gene. Errors in the molecular and tumor differentiation level analysis was able to cause the negative results in this study. Therefore further research that detected expression of p16 gene on the tongue of the oral squamous cell carcinoma patients was needed. Keywords:P16 gene, Paraffin Block, oral squamous cell carcinoma.
Perbandingan Pengaruh Pemberian Ekstrak Daun Pepaya (Carica papaya L.) dan Kurkuma terhadap Kerusakan Struktur Histologis Ginjal Tikus Putih (Rattus norvegicus) yang Diinduksi Parasetamol Dosis Toksik Alfian Noor Hakim Kusumawan; Endang Listyaningsih S; Bambang Widjokongko
Nexus Biomedika Vol 3, No 2 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Papaya (Carica papaya L.) is an interesting plant because its leaves contain chlorophyll total, chlorophyll a and chlorophyll b those are higher than the chlorophyll in the leaves of other plants. In addition, this plant also contains antioxidant components such as vitamin C, beta carotene, and iron (Fe). This study aims to compare the renoprotection power between papayas leaves extract and curcuma. Methods: This study was a laboratory experimental research. The samples were twenty four male Wistar rats those were 8-12 weeks old and 160-250 grams of body weight. Samples were divided into 6 groups randomly, each group consisted of 4 rats. The KK group was not given any treatments. The KP1 group was induced by paracetamol but it was not given any renoprotector treatments. The KP2 group was induced by paracetamol and given curcuma. The KP3, KP4, and KP5 groups were induced by paracetamol and given papaya's leaves extract at dose of 150 mg, 300 mg, and 600 mg per kg body weight respectively. Renal histological structure were assessed by counting the cell nucleus that was pyknosis, karyorrhexis, and karyolysis. Data were analyzed by One-Way ANOVA test, then followed by Least Significance Difference (LSD) test (?=0.05). Results: One-Way ANOVA test showed that p value was 0.000 (p <0.05). The results of LSD test showed the significant differences in the pair of groups: KK-KP1, KK-KP2, KK-KP3, KK-KP4, KK-KP5, KP1-KP2, KP1-KP3, KP1-KP4, KP1-KP5, KP2-KP3, KP2-KP4, KP2-KP5, KP3-KP4, KP3-KP5, and there was no significant difference in a pair of group KP4-KP5. Conclusions: Papaya's leaves extract has a better renoprotection than curcuma and increasing dose of papaya's leaves extract can improve the renoprotection. Keywords: papayas leaves, curcuma, paracetamol, damaging kidney.
The Effect of Propolis Ethanolic Extract on Serum Malondialdehyde level of mice Balb/c induced by cigarette smoke Riyan Angga Putra; Diding Heri Prasetyo; Riza Novierta Pesik
Nexus Biomedika Vol 4, No 1 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Propolis' ethanolic extract contains many kind of potent antioxidant substances, such as resveratrol, chrysin and caffeic acid in abundant quantities. These cause Propolis owns prosperity to be antioxidant and antiinflamation agent which are able to fight against free radical effect of cigarette smoke, thus lipid peroxidation won't exist and the Malondialdehyde (MDA) formation can be banned. The objective of this research was to prove the effect of Propolis' ethanolic extract on serum MDA level of mice balb/c which were induced by cigarette smoke. Methods: This was a laboratory experimental research the post test only control group design. Samples were twenty five mice, Swiss webster type (age 6-8 weeks; weight 20-30 gram) divided into 5 groups, each group had five mice. Mice for Control Group (KK) and Induction Group (KI) were administered by distilled water. While, the First Treatment Group (KP1), the Second Treatment Group (KP2) and the Third Treatment Group (KP3) were administered Propolis' ethanol extract by dose 5.6 mg, 11.2 mg and 16.8 mg per 20 g of body weight of mice respectively. All groups except KK, were induced by smoke of one cigarette stick per day before extract administration. This research was done for 14 days. On day 15th, all mice were sucked the blood, then the MDA level were measured. The data were analyzed by using One-Way ANOVA (? = 0.05) and continued by Post Hoc test (? = 0.05). Results: The results of One-Way ANOVA test showed that there was significant difference among four-groups. Post Hoc test results showed there was significant difference among all groups. Conclusion: The administration of Propolis' ethanolic extract of dose 5.6 mg/20 g of body weight of mice can decrease the serum malondialdehyde level of mice Balb/c which are induced by cigarette smoke significantly. Keywords: Propolis, Cigarette smoke, Malondialdehid level
Pengaruh Pemberian Ekstrak Etanol Buah Naga Putih (Hylocereus undatus) terhadap Kerusakan Histologis Sel Hepar Mencit (Mus musculus) yang Diinduksi Parasetamol Ardelia Kurniawan; Muthmainah .; Bhisma Murti
Nexus Biomedika Vol 1, No 1 (2012): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.858 KB)

Abstract

Background: Antioxidants in white dragon fruit are vitamin A, C, E, and polyphenol which are estimated having hepatoprotective function. The purpose of this research is to know the effect of ethanol extract of white dragon fruit (Hylocereus undatus) to the histological damage of the hepar cells of mice (Mus musculus) induced by paracetamol. Methods : This research was Randomized Controlled Trial design with sample of 28 Swiss Webster mice, male, 2-3 months old, and 20 grams of weight each. The sample was divided into 4 groups by randomization. The control group (KK) and the treatment group 1 (KP1) were fed aquadest orally 0,3 ml/20 g body weight a day for 14 days. The treatment group 2 (KP2 ) and 3 (KP3) were fed 70 and 140 mg/20 g body weight the extract of white dragon fruit a day for 14 days. On the 12th to 14th days, mice of KP1, KP2, and KP3 were treated by 5,07 mg paracetamol per 20 g body weight. On the 15th day, hepars of mice were taken and stainned with HE for histopathological study. The hepar cells of mice were observed microscopically by counting the number of necrosis cells on the centrolobuler zone. The data were analyzed by Oneway ANOVA test and Post Hoc test. Results: The average damage of hepar cells in the KK, KP1, KP2, KP3 was 21,792,69; 63,796,41; 32,072,97; 19,361,91 consecutively. The results of Oneway ANOVA test showed a significant difference in all group (p<0.001). The results of Post Hoc test showed significant differences between KK-KP1, KK-KP2, KP1-KP2, KP1-KP3, KP2-KP3 with p<0.001, except KK-KP3 with p=0.06. Conclusions : The ethanol extract of white dragon fruit (Hylocereus undatus) can prevent the histological damage of the hepar cells of mice (Mus musculus) induced by paracetamol. Keywords : ethanol extract of white dragon fruit, paracetamol, damage of hepar cells
The Correlation between Inflammatory Cells Density and NF-kB Expression in Fibroinflammatory Nasal Polyps Krisnawati Intan Suwignyo; Dyah Ratna Budiani; Fikar Arsyad Hakim
Nexus Biomedika Vol 5, No 2 (2016): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.864 KB)

Abstract

Introduction: Asian nasal polyps have more neutrophil-dominant type than Europeans (>50%), the difference of histopathology feature may develope because different etiopathogenesis so they need to treat differently. NF-kB is a transcription factor that plays an important role in corticosteroid resistance mechanism in nasal polyps, while corticosteroid is the first choice of medical treatment for nasal polyps. The aim of this research was to determined the correlation between inflammatory cells density and NF-kB expression in fibroinflammatory nasal polyps. Methods: This analytic observational study had cross-sectional design which was done on 15 paraffin embedded fibroinflammatory nasal polyps tissues from patients who had their first nasal polyps surgery and fixed with buffered formalin. The blocks were taken from Anatomical Pathology Department of RSUD Dr. Moewardi and quota sampling was used to collect the samples. Each samples was stainned using hematoxylin-eosin and NF-kB immunohistochemistry technique, both inflammatory cells and NF-kB nuclear positive were counted, then analyzed using Spearman correlation. Result: Coeficient correlation (r) between lymphocytes and nuclear NF-?B in this study was 0.129 (p = 0.647), plasma cells and nuclear NF-?B was 0.404 (p = 0.135), macrophages and nuclear NF-?B was 0.367 (p = 0.178), while neutrophils and nuclear NF-?B was 0.285 (p = 0.301). Conclusions: There is no significant correlation between inflammatory cells density and NF-?B expression in fibroinflammatory nasal polyps. Key Words: nasal polyps, nuclear NF-kB, inflammatory cells
Perbedaan Efektivitas antara Sterilisasi Dialyzer secara Manual dan Otomatis di Rumah Sakit DR.Moewardi Dezca Nindita; . Hudiyono; Endang Sutisna Sulaeman
Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Dialyzer is a critical medical equipment having high risk of microorganism contamination. Because of economical reason, dialyzer reuse is performed as first choice in Indonesia. The making of dialyzer reuse starts from rinsing, priming test and sterilization itself. Sterilization method of dialyzer that has been carried out in Rumah Sakit Dr. Moewardi is manual and automatic sterilization. Method: This was an observational study using cross sectional design. Samples taken by consecutive sampling technique were collected from the last rinsing of NaCl right before the dialyzer was used. This NaCl samples were collected from dialyzer which were sterilized manually and automatically, each, 15 samples. The difference of posistive growth of bacteria cultures of both methods was analyzed by Chi square test, while the difference of the number of colony forming unit was analyzed by independent T test. Result: Fifteen samples of the last rinsing of NaCl from dialyzer sterilized manually showed there were 10 samples positive contaminated and 5 samples negative. While from dialyzer sterilized automatically showed there were 8 samples positive contaminated and 7 samples negative. All positive samples were contaminated by positive Gram bacteria. The statistical analyses showed no significant difference of both based on quality (p = 0.456) and quantity (p = 156). Conclusion: There was bacterial contamination as many as 10 samples (66.6%) of dialyzer which was sterilized manually and 8 samples (53.3%) of dialyzer which was sterilized automatically. There was no significant difference statistically on the effectiveness between manual and automatic sterilization. Keywords: dialyzer reuse, manual sterilization, automatic sterilization.
Pengaruh Ekstrak Kulit Manggis dalam Mencegah Peningkatan Kadar Blood Urea Nitrogen pada Tikus Putih (Rattus norvegicus) Galur Wistar yang Diinduksi Gentamisin Putri Ayu Winiasih; Endang Sri Hardjanti; Nur Hafidha Hikmayani
Nexus Biomedika Vol 3, No 1 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Gentamicin, which is often used for infection treatment against aerobic Gram negative bacteria, is known having nephrotoxic effects through the inflammation mechanisms. Mangosteen pericarp has antioxidant activity and antiinflammation properties which attenuates oxidative stress, inflammatory and fibrotic pathways, hence it potentially prevents renal damages. Gentamicin-induced renal damages can be measured from elevation of blood urea nitrogen (BUN) levels. The aim of this study was to evaluate the effect of mangosteen pericarp extract on BUN levels in Wistar rats induced by gentamicin. Methods: This was an experimental study with pre- and posttest controlled group design. Thirty white male Wistar rats aged 8-12 weeks and weighed 135-170 grams were randomly divided into three groups. Normal control group (P1) was administered orally with Natrium Carboxymethylcellulose (Na CMC) 0.5%. Negative control group (P2) got intraperitoneal (i.p.) injection of gentamicin at total dose of 100 mg/kgBW. Treatment group (P3) was induced by gentamicin and 125 mg/kgBW mangosteen pericarp extract. The extract was administered orally thirty minutes after gentamicin injection for ten consecutive days. Blood samples were collected from retroorbital veins and BUN levels were measured at day 0 and day 11. Data were analyzed statistically by one-way ANOVA followed by Tukeys multiple comparison tests (?=0.05). Results: One-way ANOVA test showed no significant difference in pretest BUN levels among three groups (p=0.902). At day 11, BUN level in P2 group was significantly higher than those in P1 and P3 groups. Tukeys multiple comparison test further showed significant difference between P1-P2 and P2-P3 (p<0.001). No significant difference of BUN level was found between P1-P3 (p=0.386). Conclusions: Mangosteen pericarp extract could significantly prevent the elevation of BUN levels in Wistar rats induced by gentamicin. Keywords: mangosteen pericarp extract, gentamicin, BUN
Koinfeksi Virus GB Tipe C (GBV-C) Pasien Human Immunodeficiency Virus (HIV) Rumah Sakit Umum Daerah Dr. Moewardi di Surakarta Ratna Sariyatun; Afiono Agung Prasetyo; Yulia Sari
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Epidemiological data of GB Virus-C (GBV-C) in Indonesia are limited. Information about GBV-C coinfection among HIV patients in Dr. Moewardi General Hospital in Surakarta has also been unknown. Thus, this study is aimed to know the status of GBV-C coinfection among HIV patients in Dr. Moewardi General Hospital in Surakarta. Methods: Blood samples from 44 HIV patients in Dr. Moewardi General Hospital were subjected to viral RNA isolation. Complementary DNA (cDNA) was synthesized from the isolated RNA and used as template for nested polymerase chain reaction (PCR) detecting the 5'-non-translated region of GBV-C. The amplicons were electrophoresed in 2% agarose gel and then visualized under UV and interpreted. Results: GBV-C RNA was detected in seven samples from HIV patients (15.9%, 7/44). All patients with GBV-C coinfection were women. Conclusions: The frequency of GBV-C RNA coinfection among HIV patients in Dr. Moewardi General Hospital in Surakarta is 15.9% (7/44). Keywords: GBV-C, HIV, Surakarta
Screening The Effectiveness of Menirans Leaf Extract (Phyllanthus niruri L.), Pepaya (Carica papaya L.), and Kunyit (Curcuma longa L.) as Antidengue In Vitro Hutami Sri Ummiyati; Ratih Puspita Febrinasari; Leli Saptawati
Nexus Biomedika Vol 5, No 1 (2016): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.864 KB)

Abstract

Introduction: Dengue Haemorrhagic Fever (DHF) is an infectious disease that is caused by dengue virus. There were 71.668 DHF cases with 641 mortalities in Indonesia which was recorded from early January to mid-December 2014. Recently, DHF doesnt have a specific therapy. The aim of this study was screening about natural extracts in Indonesia as antidengue. Methods: This study was a laboratory experimental research with dengue virus serotype 2 strain New guinea C (DENV2 NGC) as a subject. This virus was infected into Huh-7 cell line. The study was conducted in 2 parts. First, dengue virus solution was mixed with natural extract and then this solution was infected to Huh-7 cell monolayer to obtain percent infectivity by FFU assay. Second,the natural extract was exposed to Huh-7 cell monolayer to obtain percent viability by MTT assay. The effective extract was selected if the extract had average percent infectivity 20% and average percent viability > 50%. Results: The percentage of viability of menirans leaf extract (Phyllanthus niruri L.), papaya (Carica papaya L.), and turmeric (Curcuma longa L.) were 96.2%, 97.5%, and 88.7% respectively. While their average percentage of infectivity were 63.9%, 10.5%, and 53.6%. Conclusion: The papayas leaf extract (Carica papaya L.) was effective as antidengue in vitro. Keywords: DENV2 NGC,dengue, meniran, papayas leaf, turmeric

Page 23 of 30 | Total Record : 292

 21   22   23   24   25 

Filter by Year

2012 2017


Reset
Filter By Issues
All Issue Vol 6, No 2 (2017): Nexus Biomedika Vol 6, No 2 (2017): Nexus Biomedika Vol 6, No 1 (2017): Nexus Biomedika Vol 6, No 1 (2017): Nexus Biomedika Vol 5, No 2 (2016): Nexus Biomedika Vol 5, No 2 (2016): Nexus Biomedika Vol 5, No 1 (2016): Nexus Biomedika Vol 5, No 1 (2016): Nexus Biomedika Vol 4, No 3 (2015): Nexus Biomedika Vol 4, No 3 (2015): Nexus Biomedika Vol 4, No 2 (2015): Nexus Biomedika Vol 4, No 2 (2015): Nexus Biomedika Vol 4, No 1 (2015): Nexus Biomedika Vol 4, No 1 (2015): Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika Vol 3, No 2 (2014): Nexus Biomedika Vol 3, No 2 (2014): Nexus Biomedika Vol 3, No 1 (2014): Nexus Biomedika Vol 3, No 1 (2014): Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika Vol 1, No 2 (2012): Nexus Biomedika Vol 1, No 2 (2012): Nexus Biomedika Vol 1, No 1 (2012): Nexus Biomedika Vol 1, No 1 (2012): Nexus Biomedika More Issue