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Current Biochemistry
Published by Institut Pertanian Bogor
ISSN : 23557877     EISSN : 23557931     DOI : -
Core Subject : Science, Education,
Biochemistry, Genetics & Molecular Biology Education
Current Biochemistry (CB) publishes the results of original research that contribute significantly to the understanding of the chemical compound and reaction that occur within living organism. Preference will be accorded to manuscripts that develop new concepts or experimantal approaches, particularly in the advancing areas of biochemistry science. Manuscripts that are primarily theoretical in nature or in the field of bioinformatics must be directed toward explaining important results previously not understood, making important predictions that can be experimentally tested, or developing segnificant advances in theory of general interest to biochemists. Submission of manuscripts in emerging areas in biochemistry, chemical biology, biophysics, proteomics, model studies and structures, cellular and molecular biology, computational biochemistry, biotechnology, and new methods development is encouraged especially if they address basic biochemical mechanisms.
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Articles 115 Documents
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Sprague Dawley Rat Spermatozoa Quality Due to Bee Pollen Consumption Hasan, Akhmad Endang Zainal; Moerfiah; Handayani, Septa Fitri
Current Biochemistry Vol. 9 No. 2 (2022)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.9.2.4

Abstract

Bee Pollen is a food that has a complete nutritional content including as a source of steroid hormones, helping to improve concentration and brain performance. This study aims to determine the effect of bee pollen on the sperm quality of male rats. Administering 1 ml of water as control (-), 1 ml/kg of Eurycoma longifolia capsule as control (+), 1 ml/kg of nano bee pollen, and bee pollen with concentrations of 0.33 ml/kg, 0.66 ml/kg, 1 ml/kg, and 1.33 ml/kg 34 times in Sprague Dawley male white rats. The mice were then operated on and examined for testicular weight, epididymal weight, sperm pH, sperm motility, sperm concentration, and sperm abnormalities. The results showed that bee pollen and nano bee pollen did not significantly affect the quality of rat spermatozoa. In examining the testicular weight of rats, 0.33 ml/kg of bee pollen had an average testicular weight greater than the control group. For sperm weight and pH of the rat epididymis, the administration of bee pollen showed the average weight and pH were lower than the control. In the examination of sperm motility, 0.33 ml of bee pollen had a higher percentage than the control, while 1 ml of nano bee pollen had the lowest percentage of motility. The results of the concentration and abnormalities of rat spermatozoa from a dose of 1 ml/kg of bee pollen had an average sperm count higher than the control which was 2,129.7 million/ml and the lowest percentage of sperm abnormalities was 8.73%.
In Silico Analysis of Active Compounds of Avocado Fruit (Persea americana Mill.) as Tyrosinase Enzyme Inhibitors Puspita, Puspa Julistia; Liliyani , Ni Putu Peggy; Ambarsari, Laksmi
Current Biochemistry Vol. 9 No. 2 (2022)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.9.2.3

Abstract

Melanocytes are an important component of skin pigmentation through their ability to produce melanin assisted by the enzyme tyrosinase which is a core enzyme in melanogenesis. The high phenolic and flavonoid compounds in the leaves, seeds and skin of avocados have potential as ligands (inhibitors) of tyrosinase related protein-1. This study aims to determine the active compounds from the leaves, seeds, and skin of avocados that play a role in inhibiting the activity of the tyrosinase enzyme in humans so that it can inhibit the formation of melanin. The research was conducted by molecular anchoring method using YASARA Structure software. The results showed that of the 68 active compounds of avocado leaves, seeds, and skins, there were 11 active compounds that had binding affinity for tyrosinase related protein-1, namely quarcetin, epicatechin, apigenin, 3-Hydroxytyrosol, scopoletin, abscisic acid, citric acid, coumaric acid. , quinic acid, vanillin, and caffeic acid. The active compound abscisic acid is the ligand with the best inhibitory potential with a bond free energy (∆G) of -2,645 kcal/mol which interacts with 17 amino acid residues. The research results can be used as a reference in in vitro and in vivo studies of the human tyrosinase enzyme.
Acetylcholinesterase Enzyme Inhibitor and Antioxidant Activities from A Mixture Extracts of Black Tea, Red Betel, Cinnamon and Curcuma Huda, Aziz Syamsul; Hasan, Akhmad Endang Zainal; Safithri, Mega
Current Biochemistry Vol. 9 No. 2 (2022)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.9.2.2

Abstract

Alzheimer's Disease (AD) is one of the consequences of impaired functioning of acetylcholine which can be hydrolyzed by the enzyme acetylcholinesterase. Alzheimer's treatment is carried out using two approaches, namely compounds (acetylcholinesterase inhibitors) and noncholinergics (antioxidants). There are commercial drugs that can slow the progression of Alzheimer's, but their use can cause excessive side effects. The use of herbal plants as a cure for Alzheimer's disease has been proven to be safer and does not cause excessive side effects. Herbal plants that can be used and developed are black tea, red betel, curcuma, and cinnamon. This study determined that extracts of black tea, red betel leaf, curcuma, and cinnamon's formula have the best antioxidant activity and level of inhibition of the acetylcholinesterase enzyme. F1 (cinnamon), F5 (cinnamon, black tea, red betel, and curcuma), F6 (cinnamon and black tea), and F11 (cinnamon, black tea, and curcuma) were the best formulations in each type of sample based on inhibitory AChE enzymes and antioxidants activity. F1 (cinnamon) is the most effective extract out of all formulations analyzed in this study. Keywords: Acetylcholinesterase; Alzheimer; Black Tea; Cinnamon; Red Betel; Curcuma
Phytochemical Screening, Total Flavonoids and Antioxidants of Kalanchoe Pinnata Linn. Leaves Qomaliyah, Eka Nurul; Indriani, Nurul; Rohma, Atika; Islamiyati, Ricka
Current Biochemistry Vol. 10 No. 1 (2023)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.10.1.1

Abstract

Kalanchoe pinnata Linn. or in Indonesia known as cocor bebek, this plants grow well in temperate and tropical regions. Cocor bebek is used to treat several diseases including skin diseases, wound healing, kidney stones, gallstones, diabetes. The pharmacological activities of cocor bebek leaves have been reported as anti-inflammatory, anti-oxidant and anti-microbial. The objective of this study is to determine the metabolite content of cocor bebek leaves, total flavonoid levels and antioxidant activity. Observations of secondary metabolites of cocor bebek leaves were carried out through qualitative secondary metabolite screening, while total flavonoid levels were carried out quantitatively UV-VIS with quercetin as a comparison, atioksidan activity through DPPH molecular inhibition testing. The results of this study indicate that cocor bebek leaves contain alkaloids, flavonoids, saponins, triterpenoids, tannins and phenols. 200gr of cocor bebek leaves obtained % yield extract is 14.63%. The total favonoid content obtained was 8.77% of quercetin, while the antioxidant inhibition of DPPH molecules obtained IC50 of 97.42 ppm.
Antibacterial Test of Endophytic Bacteria Isolated from Jawer Kotok (Coleus scutellarioides L. Benth) Against Bacillus cereus and Salmonella enteritidis Kusumawati, Dwi Endah; Bintang, Maria
Current Biochemistry Vol. 10 No. 1 (2023)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.10.1.2

Abstract

The use of synthetic antibiotics in small doses and long term against pathogenic bacteria can cause negative effects, such as resistance of pathogens and killing of good bacteria. We needs an innovation to substitute the use of synthetic antibiotics with natural antimicrobial compounds. Jawer kotok (Coleus scutellarioides L. Benth) has been used as medicinal plant and shown to be efficacious as antimicrobial because it contains flavonoids, tannins, alkaloids and saponins. Endophytic bacteria are symbiotic microorganisms that live in healthy plant tissue without harming to the host plant and may produce natural antimicrobial compounds which similar to the host plant. The aim of this study are to isolating endophytic bacteria from jawer kotok and screening their antibacterial activity against two pathogenic bacteria, namely Bacillus cereus and Salmonella enteritidis. A total of 22 bacterial isolates were obtained from C scutellarioides [L.] Benth. Based on antibacterial activity test, nine isolates shown antibacterial activity against B. cereus and S.enteritidis. BJ7 is the most potential isolate because it shown the biggest inhibition. It great possible that endophytic bacteria from jawer kotok may synthesize the same antimicrobial compounds like their host plant.
Stress Simulation Analysis on 316L Stainless Steel Bone Plate for Biomaterial Applications Ahmad Fikri; Agam Muarif; Rizka Mulyawan; Nursakinah
Current Biochemistry Vol. 10 No. 1 (2023)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.10.1.3

Abstract

Biomaterial adalah material yang digunakan untuk mengganti struktur biologis yang hilang atau sakit. Material ini harus dapat diterima oleh tubuh. Salah satu biomaterial yang paling banyak digunakan untuk aplikasi bone plate adalah stainless steel 316L. Saat diaplikasikan pada tubuh pasien, material ini akan mendapatkan beban sejajar permukaan. Beban ini merupakan beban yang cukup dominan diterima oleh bone plate stainless steel 316L. Beban yang diterima bone plate stainless steel 316L akan berubah dan bervariasi. Sehingga perlu dilakukan simulasi tegangan degan memberikan gaya tekan pada bone plate stainless steel 316L. Simulasi tegangan dilakukan dengan menggambar dan menganalisis tegangan dengan menggunakan Autodesk Fusion 360. Selanjutnya material tersebut diberikan beban berupa static load mulai dari 150 N sampai 2300 N. Hasil analisis tegangan menunjukkan distribusi tegangan terdapat ditengah dan ujung bone plate. Regangan dan perubahan bentuk menunjukkan peningkatan seiring dengan kenaikan nilai tegangan. Gaya tekan 150 N sampai 600 N merupakan beban yang aman karena masih dibawah titik luluh 170 MPa. Gaya tekan 700 N sampai 2300 N merupakan beban yang tidak aman karena sudah melebihi titik luluhnya. Hal ini akan menyebabkan material mengalami deformasi plastis dan patah.
Molecular docking: Bioactive compounds of Mimosa pudica as an inhibitor of Candida albicans Sap 3 Gholam, Gusnia Meilin; Firdausy, Iman Akhyar; Artika, I Made; Abdillah, Ramadhani Malik; Firmansyah, Ridwan Putra; Irsal, Riyan Alifbi Putera; Zahra, Hafizh
Current Biochemistry Vol. 10 No. 1 (2023)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.10.1.4

Abstract

Candida albicans (C. albicans) is a commensal microbiota that resides in humans. However, in certain cases, C. albicans can infect and cause several diseases to humans. This study aimed to investigate the interaction between Mimosa pudica bioactive compounds and C. albicans Sap 3. Molecular docking analysis was carried out using YASARA structure. The procedures involved preparation of ligands and target receptor, molecular docking, data analysis and visualization. All 3D ligands were downloaded from PubChem NCBI, while target receptor was downloaded from RCSB PDB. The interaction between Mimosa pudica bioactive compounds against Sap 3 resulted in a binding energies ranges from 5,168 – 7,480 kcal/mol and most of the interactions formed were relatively strong. Furthermore, the test ligands had contact with the catalytic residues and substrate binding site pockets S1/S2/S3/S4 on the target receptor. Bioactive compounds of Mimosa pudica have relatively good interactions in inhibiting C. albicans Sap 3
Peredaman Radikal Bebas DPPH dan Aktivitas Inhibisi α-Glukosidase oleh Ekstrak Air Jahe Merah Safithri, Mega; Syaefudin; Putri, Azka Adzkya Emalia
Current Biochemistry Vol. 10 No. 2 (2023)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.10.2.5

Abstract

Red ginger (Zingiber officinale Roxb. var. Rubrum) contains phenolic compounds that have potential to reduce blood glucose levels (antihyperglycemic) in patients with diabetes mellitus. This study aimed to determine the inhibitory activity toward α-glucosidase, antioxidant activity in reducing DPPH free radicals, and total phenolic content of aqueous extract of red ginger. The red ginger was extracted using water solvent at 100 oC for 15 minutes. Analysis of antioxidant activity, inhibitory activity of α-glucosidase, and total phenolic content using the spectrofotometry. Antioxidant activity showed that the extracts from the first, second and third replicates were not significantly different (p<0.05). α-glucosidase inhibition activity showed that the 1st replicate extract had the highest value of 97.729% and was significantly different (p<0.05) from the 2nd and 3rd replicate extracts and acarbose. The total phenolic content showed that the 2nd replicate extract had the highest value, namely 27,624 mg GAE/g extract and was significantly different (p<0.05) from the 1st and 3rd replicate extracts. The 1st, 2nd, and 3rd replicates of red ginger aqueous extract has the same antioxidant activity and equivalent glucosidase inhibitory activity as acarbose.
A Novel Plaster Dressing: A Combination of Chicken Eggshell Hydrogel Plus Button-Fungus Extract Enhances Wound Healing in Diabetic Patients Rafiqi, Akbar; Nabilatusyifa, Alivianisa; Putri, Oyi Candra Santoso; Hasanah, Ikromatul; Rahmah, Sabrina Firdayani; Andrianto, Dimas
Current Biochemistry Vol. 10 No. 2 (2023)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.10.2.1

Abstract

Diabetic patients are susceptible to severe wound conditions due to high blood sugar levels and bacterial infections. This study aims to determine the formulation and effectiveness of the hydrogel plaster combination of laying hen eggshells with button mushrooms in curing diabetic wounds. The research methods consisted of making the hydrogel, coagulation test, and antibacterial tests. Hydrogels were made with 5%, 10%, and 20% eggshell concentrations. The coagulation activity test used rabbit blood, which was added with glucose. Antibacterial test was performed using Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). This research shows that the coagulation activity of F2 and F3 formulations significantly differed from the control, with an average blood clotting time of 64.67 minutes and 63.00 minutes. The antibacterial test results produced a clear zone for S. aureus and E. coli with diameters of 10.27 mm and 9.18 mm for F2, 6.90 mm, and 11.66 mm for F3. This research concludes that hydrogel formulation 3, with 20% eggshell and 6.4% button mushroom, had the best blood clotting effect and antibacterial activity.
Peredaman Radikal DPPH oleh Minuman Mikroenkapsulasi dan Nanoenkapsulasi Sirih Merah (Piper crocatum) Tiana Fitrilia; Mega Safithri; Dinar Mutiara Hikmah
Current Biochemistry Vol. 10 No. 2 (2023)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.11.1.2

Abstract

Formulasi minuman fungsional ekstrak sirih merah dengan penambahan berbagai ekstrak rempah telah diteliti memiliki aktivitas antioksidan, namun hasil penerimaan konsumen masih rendah pada uji sensori karena rasanya pahit. Teknologi mikroenkpasulasi dan nanoenkapsulasi diketahui dapat melindungi komponen bioaktif dan dapat meningkatkan kualitas sensori pangan. Penelitian ini bertujuan untuk mengembangkan minuman fungsional berdasarkan ekstrak daun sirih merah menggunakan teknik mikroenkapsulasi dan nanoenkapsulasi dengan membandingkan ukuran partikel, kadar fenol, aktivitas antioksidan dan sensori dengan minuman ready to drink (RTD). Maltodekstrin 10% digunakan sebagai penyalut pada pembuatan minuman mikroenkapsulasi, sedangkan gum arab dan maltodekstrin digunakan untuk penyalut pada minuman nanoenkapsulasi. Ukuran partikel diuji menggunakan metode Particle Size Analyzer (PSA), total fenolik diuji dengan metode Folin- Ciocalteu, aktivitas antioksidan diuji dengan metode DPPH, dan evaluasi sensori diuji dengan tingkat hedonik (kesukaan). Hasilnya menunjukkan bahwa minuman fungsional minuman nanoenkapsulasi memiliki rata-rata ukuran partikel paling kecil yaitu sebesar 107,9 nm. Kandungan fenolik tertinggi pada minuman RTD sebesar (782,30 ± 2,54 mg GAE / g). Aktivitas antioksidan tertinggi terdapat dalam minuman mikroenkapsulasi yaitu (107,54 ± 0,83 μg / mL). Minuman fungsional nanoenkapsulasi memberikan tingkat hedonik (2,73 ± 1,19) yang lebih disukai dibandingkan minuman mikroenkapsulasi dan RTD. Teknik mikroenkapsulasi dapat meningkatkan aktivitas antioksidan dan teknik nanoenkapsulasi dapat menurunkan aktivitas antioksidan.

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