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Current Biochemistry
Published by Institut Pertanian Bogor
ISSN : 23557877     EISSN : 23557931     DOI : -
Core Subject : Science, Education,
Biochemistry, Genetics & Molecular Biology Education
Current Biochemistry (CB) publishes the results of original research that contribute significantly to the understanding of the chemical compound and reaction that occur within living organism. Preference will be accorded to manuscripts that develop new concepts or experimantal approaches, particularly in the advancing areas of biochemistry science. Manuscripts that are primarily theoretical in nature or in the field of bioinformatics must be directed toward explaining important results previously not understood, making important predictions that can be experimentally tested, or developing segnificant advances in theory of general interest to biochemists. Submission of manuscripts in emerging areas in biochemistry, chemical biology, biophysics, proteomics, model studies and structures, cellular and molecular biology, computational biochemistry, biotechnology, and new methods development is encouraged especially if they address basic biochemical mechanisms.
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Articles 111 Documents
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Kandungan Fitokimia, Total Fenol, dan Total Flavonoid Ekstrak Buah Harendong (Melastoma affine D. Don) Novilia Eka Syafitri; Maria Bintang; Syamsul Falah
Current Biochemistry Vol. 1 No. 3 (2014)
Publisher : IPB University

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Abstract

Melastoma affine plant has effect on health such as curing wound and toothache, also as an antimalarial drug. The fruit of this plant is purple and probably contain secondary metabolite compounds such as phenols and flavonoids. The total amount of both compounds may be different in unripe and ripe fruit. The aims of this research were to analyze secondary metabolite compounds and determine total phenol and total flavonoid of Melastoma affine fruit extract. The samples used in this study were unripe and ripe Melastoma affine fruits. Both of samples were extracted by three different solvents (water, 70% ethanol, and 96% ethanol) and obtained 6 extracts. Based on phytochemical test, each extract from unripe and ripe Melastoma affine fruit contained alkaloid, triterpenoid, flavonoid, phenol, and tanin. The extract with highest total phenols was 70% ethanolic extract from unripe fruit (189.56 mg/g GAE), while the extract with highest total flavonoids was 96% ethanolic extract from unripe fruit (225.50 mg/g CE). Based on this result, we conclude that unripe fruit of Melastoma affine has more total phenols and total flavonoids than ripe fruit.
Antimicrobial Activity of Allium chinense G. Don. Frans Grovy Naibaho; Maria Bintang; Fachriyan Hasmi Pasaribu
Current Biochemistry Vol. 2 No. 3 (2015)
Publisher : IPB University

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Abstract

This study aims to analyze antimicrobial activity of Allium chinense G. Don extract against Eschericia coli, Salmonella typhi, Staphylococcus aureus, Bacillus subtilis and Candida albicans, and to examine the active compounds. Allium chinense G. Don was extracted using maseration method and treated with ethanol 70% (v/v), ethanol 96% (v/v), ethyl acetate, n-hexane, and aquadest. Antimicrobial activity assay was conducted using agar difusion method and compounds analysis using Gas chromatography-mass­spectrometry (GC-MS). Antimicrobial activities assay showed that all extracts could inhibit microbial growth. Ethyl acetate extract has the highest antimicrobial activity against all the microbial test. Minimum inhibitory concentration (MIC) of ethyl acetate extract against C. albicans, B. subtilis, E. coli, S. aureus, S. typhi was 25, 100, 250, 250, 1000 mg ml-1 respectively. As many as 25 compounds were derived from GC-MS analysis and most of them were known as the antimicrobial compounds. This study revealed that Allium chinense G. Don contains biologically active compunds as antimicrobial agent particularly anti Candida.
Molecular Interaction Analysis of COX-2 against Curcuminoid and Xanthorizol Ligand as Anti Breast Cancer using Molecular Docking Ridho Pratama; Laksmi Ambarsari; Tony Ibnu Sumaryada
Current Biochemistry Vol. 2 No. 3 (2015)
Publisher : IPB University

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Abstract

Breast cancer is one of the most common cancer in the world that occurs in women. Thedevelopment process of cancer is regulated by a variety of pathways that involve various enzymes. COX-2 is an enzyme involved in the inflammatory process in further stages that will play importantroles in breast cancer cells progression. The uses of natural compound from plants give new hope forbreast cancer treatment with minimal side effects. Temulawak is a potential breast cancer drugs because it contains curcuminoid and xanthorizol. Curcumin and xanthorizol has been reported to have chemopreventive effect on colon cancer development. The drug that has same functions of these compounds needed to be examined with various approaches. One of the approach used in this research is molecular docking. Based on ligand analysis with Lipinski and toxicity test using ADMET, curcuminoid and xanthorizol met criteria as medicinal compounds. Curcumin had the highest binding affinity(?G) with the value -9.3 kcal/mol but still under commercial drug celecoxib binding affinity (?G) = -12,5. There were three hydrogen bonds in amino acid Arg106 and Tyr341 His75 which were amino acids in the active side of COX-2. There were 15 amino acids that have similar ties with commercial drug celecoxib. Based on the binding affinity and binding similiarity, curcuminoid and xanthorizol were predicted as compounds that have potential as competitive inhibitor of COX-2 enzyme.
The In Vitro Antibiofilm Activity of Water Leaf Extract of Papaya (Carica papaya L.) against Pseudomonas aeruginosa Ekajayanti Kining; Syamsul Falah; Novik Nurhidayat
Current Biochemistry Vol. 2 No. 3 (2015)
Publisher : IPB University

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Abstract

Pseudomonas aeruginosa is one of opportunistic pathogen forming bacterial biofilm. The biofilm sustains the bacterial survival and infections. This study aimed to assess the activity of water extract of papaya leaves on inhibition of cells attachment, growth and degradation of the biofilm using crystal violet (CV) biofilm assay. Research results showed that water extract of papaya leaves contains alkaloids, tanins, flavonoids, and steroids/terpenoids and showed antibacterial activity and antibiofilm against P. aeruginosa. Addition of extract can inhibit the cell attachment and was able to degrade the biofilm of 40.92% and 48.058% respectively at optimum conditions: extract concentration of 25% (v/v), temperature 37.5 °C and contact time 45 minutes. With a concentration of 25% (v/v), temperature of 50 °C and the contact time of 3 days, extract of papaya leaves can inhibit the growth of biofilms of 39.837% v/v.
Water Extract Activity of Papaya Leaf as Antibiofilm against Escherichia coli Livia Rhea Alvita; Syamsul Falah; Novik Nurhidayat
Current Biochemistry Vol. 2 No. 3 (2015)
Publisher : IPB University

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Abstract

Biofilms are significant hazards in the food industry. The presence of Escherichia coli biofilms in food industry is potentially causing food spoilage that will shorten the shelf life as well as lead to the spread of disease through food. The purpose of this study is to determine the phytochemical compound of aqueous extract of papaya leaves and to determine its activity on inhibition of cell attachment and growth, and on degradation of the biofilm using Micro-titter Plate Biofilm Assay. The results phytochemical test showed that papaya leave extract contains alkaloid, tanin, flavonoid, and steroid and have anti-biofilm activity. Papaya leave extract with highest activity caused biofilm degradation of 48,99%. The optimum temperature of biofilm degradation (>52,5%) by papaya leaves extract was around 35oC - 40oC. The optimum contact time and extract concentration were 57-60 minutes and 75-100%, respectively.
Delignifikasi Batang Kayu Sengon oleh Trametes versicolor Azmi Azhari; Syamsul Falah; Laita Nurjannah; Suryani Suryani; Maria Bintang
Current Biochemistry Vol. 1 No. 1 (2014)
Publisher : IPB University

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Abstract

Delignification is a lignin degradation, a preliminary process in industries that used cellulose containing substrates. Sengon logs are often used for the material in pulp industry because it has high levels of cellulose and low level of lignin. The aim of this study was delignification of sengon logs by using T.versicolor. The methods used include observation growth of T.versicolor compared with Phanerochaete chrysosporium, the rate of of lignin degradation (black liquor), delignification of sengon logs using T.versicolor and the chemical assay of sengon logs before and after delignification. The results of this study showed that delignification by T.versicolor was faster compared to P.chrysosporium based on the rate of lignin degradation (black liquor). The result showed that delignification by T.versicolor at room temperature reduced lignin of sengon logs by 37.31% within 20 days. Chemical assay performed on delignified sengon wood showed decreased level of ethanol benzene, soluble extractive substances, holocellulose, and cellulose and an increase of hemicellulose level.
Deodorization of Latex Waste and Decolorization of Textile-Coloring Agent by Omphalina sp. using Batch and Continuous Methods Erna Puspasari; I Made Artika; Tri Panji
Current Biochemistry Vol. 3 No. 1 (2016)
Publisher : IPB University

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Abstract

Generally industrial waste water is a pollutant to environment as it produces strong odor and color. Omphalina sp is one of white rot fungi that can be used as an odor and color effluent reducer. Omphalina sp has laccase enzyme that plays role in deodorization and decolorization. The aim of this research was to determine the best method in deodorization and decolorization among batch and continous (pack bed flow, biotray, and rotary contactor) methods. The results of deodorization and decolorization showed that the rotary contactor method was better than the batch, pack bed flow and biotray methods. At dye concentration of 50 ppm, after 24 hours treatment, the absorbance value for batch, pack bed flow, biotray, and rotary contactor methods was 0.520, 0.423, 0.425, and 0.357, respectively. At dye concentration of 150 ppm, after 24 hour treatment, the absorbance value for batch, pack bed flow, biotray, and rotary contactor methods was 0.709, 0.629, 0.658, and 0.592, respectively. At dye concentration of 50 ppm, percentage of dye absorption after 24 hour treatment for batch, pack bed flow, biotray, and rotary contactor methods was 20.550%, 35.447%, 35.141% and 45.531% respectively. At dye concentration of 150 ppm, percentage of dye absorption after 24 hour treatment for batch, pack bed flow, biotray, and rotary contactor methods was 7.320%, 17.843%, 13.987%, and 22.614%. The qmaks value at dye concentration of 50 ppm after 24 hour treatment for batch, pack bed flow, biotray, and rotary contactor methods was 0.020, 0.076, 0.083, and 0.124 respectively. qmaks value at dye concentration of 150 ppm after 24 hour treatmeny for batch, pack bed flow, biotray, and rotary contactor methods was 0.009, 0.077, 0.046, and 0.100 respectively. Odor scale for batch, pack bed flow, biotray, and rotary contactor methods decreased from 5 to 2.9, 1.4, 1.6 and 1.1 respectively.
Simulasi Docking Senyawa Kurkumin dan Analognya Sebagai Inhibitor Reseptor Androgen pada Kanker Prostat Arwansyah Arwansyah; Laksmi Ambarsari; Tony I Sumaryada
Current Biochemistry Vol. 1 No. 1 (2014)
Publisher : IPB University

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Abstract

Curcumin, the major compound of Curcuma longa L, has been proven to have the toxicity effect on prostate cancer cell. This research was aimed to study the affinity and interaction of curcumin and its analogs as compettitive inhibitor to androgen hormon before working in vitro/in vivo research. Curcumin and its analogs were transformed into 3D structure, then docked to androgen receptor (3B67). The data of Gibbs energy (?G) value showed stability interaction between ligand and androgen receptor residues. The docking results showed that curcumin and its analogs have potential as inhibitor on androgen receptor. Based on results ?G score, analog 4 (1,7-bis-(3,4-dihydroxy-phenyl)-hepta-1,6-diene-3,5-dione) has highest potential as the inhibitor for androgen receptor.
Characterization and Toxicity of Temulawak Curcuminoid Nanoparticles Riki Riki; Popi Asri Kurniatin; Laksmi Ambarsari; waras Nurcholis; Latifah K Darusman
Current Biochemistry Vol. 3 No. 1 (2016)
Publisher : IPB University

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Abstract

Temulawak exctract contains curcuminoids which have anticancer potential. However, clinical application of curcuminoid has been limited due to its low bioavailability. One of the efforts that can be developed to solve this problem is incorporated curcuminoids into Solid Lipid Nanoparticles (SLN) carriers system. The objective of this study was to characterize dan evaluate anticancer potential of temulawak ethanolic fraction nanoparticles. HPLC method was used to determined curcuminoids content of temulawak ethanolic fraction. Characterization indicators like polydispersity index, particle size, morpholgy, and entrapment efficiency. HPLC chromatogram has shown of curcumin, demethoxycurcumin, and bisdemethoxycurcumin were found in temulawak ethanolic fraction. The particle size of nanoparticles obtained in this study was 648.4 ± 95 nm with polydispersity index value of 0.216. A uniform size distribution of nanoparticles as observed by Transmission Electron Microscopy (TEM). The entrapment efficiency of curcuminoid in nanoparticles was about 29.8%. Based on results of BSLT obtained temulawak extract Lethal Concentration (LC50) value of 213.24 ppm and 828.78 ppm of nanoparticles.
Induksi dan Karakter Pertumbuhan Kalus Triploid dari Endosperma Avokad (Persea americana Mill.) Edy Sukmara; Lazarus Agus Sukamto; Maria Bintang
Current Biochemistry Vol. 1 No. 1 (2014)
Publisher : IPB University

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Abstract

The concentration of the Plant Growth Regulators (PGR) medium as well as endosperm viability determines the success of triploid avocado callus. This study was aimed to explore the best size of diameter fruit and the most responsive concentration for the induction of endosperm avocado callus. The study consisted of two phases. The first stage is to determine the best diameter size of avocado and concentration of the culture medium that gave the fastest response to be endosperm callus. The second stage used flow cytometer to obtain triploid of avocado endosperm callus. This study used four size of fruit group diameter, that A=(0.30-0.50), B=(0.51-1.00), C=(1.01-1.50) and D=(1.51-2.20) in cm. Factorial experiment with four replications was arranged in a completely randomized design. Murashige and Skoog medium (MS) was used in this culture with two PGR that the Picloram and 2.4-D. Each with concentration : 0.5, 1.0, 2.0 and 4.0 in mg/l. The most responsive diameter fruit size was group B (0.30 – 0.50 cm) with the fastest callus growth average was 7.67 weeks after culture (WAC). The most responsive concentration for endosperm callus induction was Picloram 2 mg/l, growth in 5.08 WAC. The endosperm callus growth successfully t done by the inclusion of avocados embryos. The best interaction between fruits diameter and concentration for callus avocado induction used fruits diameter A with picloram 2 mg/l. The endosperm callus were measured by using flow cytometer resulted in avocado triploid callus with region range (RN) Mean value wer 302.01, 296.60, and 298.51 respectively. While the avocado leaf control resulted in 198.82 diploid plant.

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