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Jurnal Biosains Pascasarjana
Published by Universitas Airlangga
ISSN : 14121433     EISSN : 26214849     DOI : http://dx.doi.org/10.20473/jbp.v22i1.2020.1-10
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology
Jurnal Biosains Pascasarjana is published not only for the publication of research results from graduates, as one of the graduation requirements but also for public that contains a discussion of the natural content, responses of living things, and their environment.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 9 Documents
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Search results for , issue "Vol. 24 No. 1SP (2022): JURNAL BIOSAINS PASCASARJANA: SPECIAL ISSUE" : 9 Documents clear
A CYTOKINE RESPONSE IN BRUCELLA ABORTUS BOVINE INFECTION: LITERATURE REVIEW septyana eka rahmawati; RIAN ANGGIA destiawan; novarina sulsia ista'in ningtyas; Mutia Hariani Nurjanah
Jurnal Biosains Pascasarjana Vol. 24 No. 1SP (2022): JURNAL BIOSAINS PASCASARJANA: SPECIAL ISSUE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v24i1SP.2022.1-12

Abstract

Brucella Abortus infection in cattle is an infection that has a strategic effect on society, especially the economic sector. This disease is caused by gram-negative bacteria, namely Brucella abortion. Treatment of Brucella Abortus Infection in cattle is still not effective. Therapy based on the immune response caused by Brucella Abortus Infection, especially the cytokine response, is an effective choice considering that each type of cow has a different immune response in dealing with Brucella Abortus Infection. The purpose of writing this literature review is to identify cytokines that play a role in Brucella Abortus Infection in cattle. Method: automated PUBMED database search using keywords “Brucella Abortus Bovine”, “Cytokine”, and “Brucellosis”. Results: 11 articles that are relevant and meet the inclusion criteria to analyze the cytokines that play a role in Brucella Abortus Infection in cattle. Conclusion: Cytokines that play a role in Brucella Abortus infection in cattle are generally IL-1, IL-6, IL-12, IL-8, TNF-α, and IFN-γ.
DETECTION Escherichia coli IN DRINKING WATER SOURCES IN CHICKEN FARMING IN NARMADA DISTRICT, LOMBOK BARAT REGENCY novarina sulsia ista'in ningtyas; alfiana laili dwi agustin; septyana eka rahmawati
Jurnal Biosains Pascasarjana Vol. 24 No. 1SP (2022): JURNAL BIOSAINS PASCASARJANA: SPECIAL ISSUE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v24i1SP.2022.74-83

Abstract

Water plays an important role in human life. One of the sources of water used by the community is a source of water that comes from wells. The water source in the poultry area in Narmada is not only used for livestock needs but is utilized and consumed by the community especially farmers around the water source. The distance between the water source and the poultry area is less then 15 meters which is the cause of contamination. Research the aimed to detect contaminating bacterial in water source in Poultry of Narmada Regency. The study used a survey method with cross sectional study approach which was carried out in September 2022. Sample were taken using sterile bottles which were then cultured in EMBA, NA and biochemical test. The result of this study showed that there were four samples contaminated with E.coli which was marked with a metallic green on EMBA and the show basil and red colors under microscope.
EFFECT OF BLACK GRAPE EXTRACT ON CD4+ and CD8+ EXPRESSION IN MICE INFECTED WITH Salmonella typhimurium rian anggia destiawan; septyana eka rahmawati; anas fadli wijaya; Ahdiah Imroatul Muflihah; Mutia Hariani Nurjanah; Sholihatil hidayati; Ni Ketut Yuliana sari
Jurnal Biosains Pascasarjana Vol. 24 No. 1SP (2022): JURNAL BIOSAINS PASCASARJANA: SPECIAL ISSUE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v24i1SP.2022.54-63

Abstract

Salmonelosis is a disease caused by the Salmonella sp. that causes a decrease in CD4+ expression. Black grape can boost the immune system through CD4+/CD8+ proliferation. The purpose of this study was to evaluate the activity of black grape extract to CD4+ and CD8+ expression in mice infected with Salmonella typhimurium. Research method is extract of black grapes, Salmonella typhimurium 108 infection, bacterial evaluation, extract therapy, flowcytometery examination, and 95% Anova test. The results of Anova test showed that the expression of CD4+ and CD8+ is not different. Biologically, an increase in CD4+ and CD8+ expression at doses of 100 mg/KgBB. A decrease in CD4+ and CD8+ expression at doses of 200 mg/KgBB and 400 mg/KgBB. Conclusion, biologically, black grape extract can increase the expression of CD4+ and CD8+ at low doses, as well as may decrease the expression of CD4+ and CD8+ at moderate to high doses.
THE RELATIONSHIP BETWEEN GASTROINTESTINAL WORMS PREVALENCE AND AGE OF CIDOMO DRILLING HORSE IN MATARAM CITY syafindri syafindri; kholik kholik; kunti tirtasari; maratun janah
Jurnal Biosains Pascasarjana Vol. 24 No. 1SP (2022): JURNAL BIOSAINS PASCASARJANA: SPECIAL ISSUE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v24i1SP.2022.30-38

Abstract

Kuda penarik cidomo yang digunakan sebagai alat transportasi di Kota Mataram sangat rentan terhadap penyakit cacingan karena hidup di jalan dengan pemeliharaan secara tradisional yang akan berdampak pada kesehatan kuda. Telah dilakukan penelitian cross-sectional pada kuda penarik cidomo di Kota Mataram pada bulan Februari sampai Maret 2021. Penelitian ini bertujuan untuk mengetahui hubungan prevalensi cacingan saluran cerna dengan umur kuda penarik cidomo di Kota Mataram. Sebanyak 74 feses kuda penarik cidomo dikumpulkan langsung dari dubur kuda untuk pemeriksaan telur cacing guna mengukur prevalensi cacingan saluran cerna. Pemeriksaan telur cacing pada feses dilakukan di Laboratorium Equine Clinical Skill Center Fakultas Kedokteran Hewan, Universitas Pendidikan Mandalika dengan metode sedimentasi dan flotasi. Hubungan prevalensi cacingan saluran cerna dengan umur kuda penarik cidomo dianalisis dengan uji Pearson dengan α = 0,05. Hasil penelitian menunjukkan bahwa prevalensi cabut cidomo yang terinfeksi cacing adalah 76,1% (53/7). Telur cacing yang teridentifikasi adalahStrongylus spp, Trichostrongylus spp, Cyathostoma spp, Ostertagia spp, dan Parascaris equorum . Hubungan prevalensi infeksi cacing saluran cerna dengan umur kuda penarik cidomo tidak bermakna dengan p = 0,22.
PHENOTYPIC DETECTION OF Escherichia coli PRODUCING Extended Spectrum Beta Lactamases (ESBLs) IN THE REPRODUCTIVE TRACT BALI COW Lalu Purnama Tasyakusuma; kholik kholik; maratun janah; alfiana laili dwi agustin; septyana eka rahmawati
Jurnal Biosains Pascasarjana Vol. 24 No. 1SP (2022): JURNAL BIOSAINS PASCASARJANA: SPECIAL ISSUE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v24i1SP.2022.64-73

Abstract

Escherichia coli bacteria that are resistant to β-lactam antibiotics and may be able to produce Extended-spectrum β-lactamase (ESBLs) have been found in the reproductive tract of Bali cattle. Escherichia coli that produce ESBL will cause antimicrobial resistance (AMR) which has become a global health problem. The purpose of this study was to detect phenotypically the presence of Escherichia coli that produces ESBL from the reproductive tract of Bali cattle. This research was conducted in February 2022 in Lando Village, Terara District, East Lombok Regency using 8 female Bali cattle on smallholder farms. Reproductive fluid samples were collected using a plastic sheet gun which was inserted into the Brain Infusion Heart (BHI) media and incubated at the Public Health and Calibration Laboratory of West Nusa Tenggara Province. The samples were then cultured in EMBA media for the isolation of Escherichia coli. The isolated Escherichia coli were then identified by gram staining and biochemical tests based on Bergey's manual of determinative bacteriology. The identified Escherichia coli were screened using the disc diffusion method using the antibiotics Penicillin G10U, Ceftazidime 30 μg, and Cefotaxime 30 μg followed by a confirmation test using the double disk synergy test (DDST) method. The results of the screening test showed that only 12.5% of Escherichia coli (1 out of 8 samples) were resistant to Penicillin G, but they had not been detected to produce ESBL phenotypically.
POTENTIAL OF SEAWEED (Eucheuma cottonii) EXTRACT AS A HEPATOPROTECTOR IN RABBIT (Oryctolagus cuniculus) INDUCED ORGANOPHOSPHATE PESTICIDES Alza Hamdi Putra; kholik kholik; novarina sulsia ista'in ningtyas
Jurnal Biosains Pascasarjana Vol. 24 No. 1SP (2022): JURNAL BIOSAINS PASCASARJANA: SPECIAL ISSUE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v24i1SP.2022.21-29

Abstract

The use of organophosphate pesticides in developing countries is already widespread and can contaminate animal feed or human food ingredients which has an impact on liver damage and other organs. The use of seaweed containing polyphenols as a hepatoprotector for organophosphate poisoning has not been widely used by the public. The purpose of this study was to determine the potential of seaweed extract as a hepatoprotector in organophosphate-induced rabbits as seen from serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) levels. This study used a complete randomized design with 20 male rabbits as experimental animals which were divided into 4 treatments with 5 repetitions, namely: P0 was the control group which was only given distilled water, P1 was the group given seaweed extract at a dose of 200 mg/Kg BW, P2 is the group given seaweed extract at a dose of 400 mg/KgBB and P3 is the positive control group which is not given seaweed extract and distilled water. On the 15th day the P1, P2, and P3 groups were given profenofos pesticide at a dose of 50 mg/kg BW. The results showed a very significant difference (p-value = 0.02, α = 0.05) in SGOT levels between the P0 control group (135.4 ± 67.00 U/L) and the P1 treatment group (41.8 ± 17.45 U/L), P2 (25.8 ± 8.75 U/L), and the positive control group P3 (70 ± 29.04). The results of the study also found that there was no significant difference in SGPT levels in the P0 control group and the P1, P2, and P3 control groups. Giving seaweed extract with different doses provides a hepatoprotector effect by reducing the release of SGOT into the blood.
DETECTION OF CD4+ T-LYMFOCYTE ADAPTIVE IMMUNITY CELL EXPRESSION IN MICE INFECTED WITH Salmonella typhimurium Mutia Hariani Nurjanah
Jurnal Biosains Pascasarjana Vol. 24 No. 1SP (2022): JURNAL BIOSAINS PASCASARJANA: SPECIAL ISSUE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v24i1SP.2022.39-43

Abstract

Salmonella typhi is a gram-negative, intracellular facultative bacterium that can live and even reproduce in macrophages, resistant to lysosomal enzymes, which has the ability to prevent phagosome-lysosome fusion, making it difficult to kill. stimulates the immune response in the host (Abbas et alll, 2016; Tores et alll, 2000). White turmeric (Curcuma zedoaria) is one of the herbal plants used as an immunomodulator. Immodulators in white turmeric substances function to increase the function of phagocytosis in macrophages to destroy Salmonella typhimurium bacteria. The purpose of this study was to determine the difference between white turmeric extract as an immunomodulator on the number of CD4+ T lymphocytes in BALB/c mice infected with Salmonella typhimurium. This research is a true experimental research with a posttest only control group design. Mice were divided into 5 groups consisting of negative control group, positive control and test group. Analysis of CD4+ lymphocyte data showed a significant difference p=0.013, this indicated that administration of white turmeric extract (Curcuma zedoaria) had an effect on the number of CD4+ T lymphocytes in BALB/c mice infected with Salmonella typhimurium
DETECTION OF ANTIBIOTIC RESISTANT IN Escherichia coli FROM THE REPRODUCTIVE TRACT OF BALI CATTLE ON SMALLHOLDER FARM Kholik Kholik
Jurnal Biosains Pascasarjana Vol. 24 No. 1SP (2022): JURNAL BIOSAINS PASCASARJANA: SPECIAL ISSUE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v24i1SP.2022.44-53

Abstract

Escherichia coli originating from animals, humans, and the environment can spread antibiotic-resistant genes and can encourage antimicrobial resistance (AMR) which is a global health problem. The purpose of this study was to detect the presence of Escherichia coli which is resistant to several antibiotics from the reproductive tract of Bali cattle on smallholder farms on the island of Lombok. This research is a cross-sectional study conducted from March to June 2021 using 8 female Bali cattle that experience reproductive disorders on community farms in Lando Village, East Lombok Regency to collect their reproductive tract fluids using an artificial insemination plastic sheet gun. Reproductive fluid samples are placed on BHIB (Brain Heart Infusion Broth) medium. Escherichia coli cultures were carried out on Eosin Methylene Blue Agar (EMBA) and identified by Gram staining and biochemical tests conducted at the Public Health and Calibration Laboratory, West Nusa Tenggara Province. Antibiotic sensitivity test on isolated Escherichia coli was carried out by disc diffusion method using 5 antibiotics including Penicillin G 10U, Oxytetracycline 30 µg, Gentamicin 10 µg, Tetracycline 30 µg, and Cefotaxime 30 µg. The results showed that 2 (two) 25% Escherichia coli bacteria were successfully cultured on EMBA media and isolated from 8 samples of the reproductive fluids of Bali cattle that were collected. The results of the Escherichia coli sensitivity test to antibiotics found that Escherichia coli samples were 100% resistant to Penicillin G, 100% resistant to Oxytetracycline, 100% resistant to Gentamicin, and 50% resistant to Tetracycline, and 100% resistant to Cefotaxime from 2 isolated Escherichia coli. This explains that Bali cattle in community farms have the potential to spread Escherichia coli which has an impact on the emergence of AMR.
RELATIONSHIP BETWEEN HbA1C AND eGFR IN DIABETES MELLITUS (DM) PATIENTS FOLLOWING PROLANIS AT ULTRA MEDICA TULUNGAGUNG CLINIC LABORATORY taufik salis syaifuddin; Mutia Hariani Nurjanah; Nurul Chamidah Kumalasari; Wimbuh Tri widodo
Jurnal Biosains Pascasarjana Vol. 24 No. 1SP (2022): JURNAL BIOSAINS PASCASARJANA: SPECIAL ISSUE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v24i1SP.2022.13-20

Abstract

Diabetes mellitus is a condition of abnormal blood glucose metabolism characterized by prolonged hyperglycemia and elevated HbA1c levels as a standard test for diabetes mellitus. HbA1c describes the level of sugar in the blood for the last 3 months. One of the complications of diabetes mellitus is kidney damage. The state of kidney function can be described from the GFR (Glomerular Filtration Rate) examination as a standard examination for abnormalities in kidney disease. The purpose of this study was to determine the relationship between HbA1c levels and eGFR values ​​in people with diabetes mellitus. The design of this study was a cross-sectional approach to 30 samples of DM patients who took Prolanis at the Ultra Medica Tulungagung Clinical Laboratory. The results showed that the average HbA1c level in all samples was 6.2% and the average eGFR value was 88.7 ml/min/1, 73m2. The Pearson correlation test obtained a ρ value = 0.000 so that it can be concluded that there is a relationship between HbA1c levels and eGFR values ​​in people with diabetes mellitus who take Prolanis at the Ultra Medica Clinical Laboratory Tulungagung.

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