Garuda - Garba Rujukan Digital

Kandungan Senyawa Bioaktif dan Aktivitas Biologis Ekstrak Daun Rhizophora apiculata Asal Perairan Teluk Awur, Jepara Mirsa Septiana Mutik; Mada Triandala Sibero; Widianingsih Widianingsih; Subagiyo Subagiyo; Rudhi Pribadi; Dwi Haryanti; Ambariyanto Ambariyanto; Retno Murwani
Jurnal Kelautan Tropis Vol 25, No 3 (2022): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v25i3.14287

Rhizophora apiculata is a type of mangrove that has the ability to adapt to extreme environmental conditions such as temperature, low oxygen levels, and high salinity. This adaptability affects the production of secondary metabolites. Information about the antibacterial activity of this mangrove against MDR (Multi-Drug Resistant) bacteria is still very limited. The content of secondary metabolites produced by mangrove R. apiculata is also expected to affect antioxidant activity against free radicals. The purposes of this study were to examine the presence of bioactive compounds by phytochemical tests and to evaluate the antibacterial activity against MDR bacteria such as Pseudomonas aeruginosa, Escherichia coli, Bacillus cereus, and Bacillus subtilis; and antioxidants property of R. Apiculata leaves that were collected from Teluk Awur, Jepara. The leaves were extracted using the multilevel maceration method with solvent sequence n-hexane, ethyl acetate and methanol. Metabolite finger printing using TLC method was carried out to detect the presence of alkaloids, flavonoids, saponins, phenolics, quinones, steroids, and triterpenoids. The antibacterial test was carried out using agar well diffusion method while the antioxidant test was carried out using the DPPH method. The results of the phytochemical test showed that there were groups of alkaloids and steroids in the n-hexane solvent; alkaloids, phenolics, and steroids in ethyl acetate solvent; as well as alkaloids, flavonoids, phenolics, and saponins in methanol solvents. The results of this study indicate that R. apiculata from Teluk Awur Coastal Waters, Jepara had no potential as an antibacterial against MDR (Multi-Drug Resistant) bacteria, however the methanol extract has the potential to be used as an antioxidant with an IC50 value of 85.999 ppm. The bioautography showed that compounds from the phenol group, flavonoids, triterpenoids and b-carotene pigments acted as antioxidant agents in the leaf extract of R. apiculata. Rhizophora apiculata merupakan salah satu jenis mangrove yang memiliki kemampuan beradaptasi terhadap kondisi lingkungan ekstrem seperti suhu, kadar oksigen rendah dan salinitas tinggi. Kemampuan beradaptasi tersebut mempengaruhi produksi matabolit sekunder. Informasi mengenai kemampuan aktivitas antibakteri mangrove jenis ini melawan bakteri MDR (Multi Drug Resistent) masih sangat terbatas. Kandungan metabolit sekunder yang dihasilkan mangrove R. apiculata ini juga diharapkan dapat mempengaruhi aktivitas antioksidan melawan radikal bebas. Tujuan dari penelitian ini adalah untuk mengkaji kandungan senyawa bioaktif serta aktivitas biologis berupa antibakteri melawan bakteri MDR Pseudomonas aeruginosa, Escherichia coli, Bacillus cereus dan Bacillus subtilis; dan antioksidan dari ekstrak daun mangrove R. apiculata asal perairan Teluk Awur, Jepara. Sampel diekstraksi menggunakan 3 pelarut berbeda (n-heksana, etil asetat dan metanol) dengan metode maserasi bertingkat. Analisis metabolit sidik jari dilakukan menggunakan plat KLT untuk mengetahui kandungan senyawa alkaloid, flavonoid, saponin, fenolik, kuinon, steroid dan triterpenoid. Uji antibakteri dilakukan menggunakan metode sumuran sedangkan uji antioksidan dilakukan menggunakan metode DPPH. Hasil uji fitokimia menunjukkan terdapat golongan senyawa alkaloid dan steroid pada pelarut n-heksana; alkaloid, fenolik dan steroid pada pelarut etil asetat; serta alkaloid, flavonoid, fenolik dan saponin pada pelarut metanol. Hasil penelitian ini mengindikasikan bahwa R. apiculata asal perairan Teluk Awur, Jepara tidak potensial sebagai antibakteri melawan bakteri MDR (Multi Drug Resistent), akan tetapi ekstrak metanol potensial digunakan sebagai antioksidan dengan nilai IC50 85,999 ppm. Tahapan bioautografi menunjukkan bahwa senyawa dari golongan fenol, flavonoid, triterpenoid dan pigmen b-karoten berperan sebagai agen antioksidan pada ekstrak daun R. apiculata.

Perubahan Luas Terumbu Karang di Pulau Panjang, Jepara Suryono Suryono; Ambariyanto Ambariyanto; Munasik Munasik; Raden Ario; Ibnu Pratikto; Nur Taufiq-SPJ; Syahrial Varrel Canavaro; Tiara Anggita; Prayogi Prayogi; Eny Supryhatun
Jurnal Kelautan Tropis Vol 25, No 3 (2022): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v25i3.10249

The coral reefs of Panjang Island, Jepara have been under pressure both by natural factors and by human (anthropogenic) activities, which have resulted in the degradation coral reef area. Research on changes in coral reefs is needed, but the constraints are time, cost, and labor. Remote sensing interpretation of satellite image data is an alternative to determine changes in the area of coral reefs. The use of multitemporal image data can be applied to analyze changes that occur, in this study using four Landsat image data with the acquisition of 2001,2005, 2010, and 2019. The purpose of this study was to determine changes in the area of coral reefs in Panjang Island for the period 2001-2019. The research material used is Landsat 7 and Landsat 8 imagery. The research method is a survey method with a multispectral classification of the maximum possibilities for extracting coral reef cover data from multitemporal images that have been corrected geometrically and radiometrically. A field survey (ground check) was conducted to determine the percentage mapping accuracy. Data on coral reef cover were overlaid to obtain the results of changes in the area of coral reefs for the period 2001-2019. The results showed that the coral reefs in Panjang Island had undergone extensive changes, namely: The area of living coral reefs decreased to 64,34%, with a change rate of 0.17 HaYear-1from 2001-2019. In 2001, the distribution of live coral reefs was seen on the northeast, east, south, and west sides of Panjang Island then reduced coral reefs area, finally left on the northeast and south sides of the island in 2019. Terumbu karang di Pulau Panjang ,Jepara telah mengalami tekanan baik oleh peristiwa alam maupun oleh aktivitas manusia (antropogenik), yang menyebabkan terjadinya degradasi luasan areal. Penelitian tentang perubahan luas terumbu karang sangat diperlukan, namun kendaalanya adalah waktu lama, biaya serta tenaga yang banyak. Penginderaan jauh interpretasi data citra satelit adalah salah satu alternatif guna mengetahui perubahan luas terumbu karang.Pemanfaatan data citra multitemporal dapat diterapkan untuk menganalisis perubahan yang terjadi, dalam penelitian ini menggunakan empat data citra landsat dengan akuisisi tahun 2001,2005, 2010 dan 2019. Tujuan penelitian ini adalah untuk mengetahui perubahan luas terumbu karang di perairan Pulau Panjang perode tahun 2001-2019. Materi Penelitian yang dipergunakan adalah citra Landsat 7 dan Landsat 8.Metode penelitian adalah metode survei dengan klasifikasi multispektral dari kemungkinan maksimum untuk ekstraksi data tutupan terumbu karang dari citra multitemporal yang telah dikoreksi secara geometris dan radiometrik.survei lapangan (ground check) dilakukan untuk mengetahui persentase akurasi pemetaan. Data luas tutupan terumbu karang dioverlay guna mendapatkan hasil perubahaan luas terumbu karang periode tahun 2001-2019. Hasil penelitian menunjukan bahwa Terumbu karang di Pulau Panjang telah mengalami perubahan luas yaitu 64,34% dengan laju penurunan sebesar 0,17 HaTh-1dari Tahun 2001-2019. Pada tahun 2001 sebaran terumbu karang hidup terlihat di sisi timur laut, timur, selatan dan dan barat dari Pulau Panjang, kemudian semakin mengecil areanya, hingga tersisa pada sebelah sisi timur laut dan selatan pulau saja pada tahun 2019.

Alginate from Sargassum sp. Improve the Hematology Performance and Oxygen Tolerance Exposure of Lates calcarifer Ervia Yudiati; Ali Djunaedi; Nuril Azhar; Rabia O. Alghazeer
Jurnal Kelautan Tropis Vol 25, No 3 (2022): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v25i3.16217

Indonesia's fish production is abundant, especially in aquaculture. Lates calcarifer is farmed fish species. The failure of L. calcarifer cultivation due to disease problems. We are utilizing a natural compound derived from tropical Sargassum sp. extracts, namely alginate. This study aims to determine and analyse the supplementation of alginate in the diet by oral administration to improve the fish's hematological performance and oxygen stress tolerance. There were one control and three treatments (2 g.kg-1, 4 g.kg-1, and 8 g.kg-1 alginate/feed). The experiment was a completely randomized design with three replications. Fish were reared in a 350 L fiber tank for 12 days at a density of 20 ind.tank-1. Stress oxygen tolerance was applied by rearing the 10 fish in 12 L fully plastic-wrapped containers. The parameters test analyzed were phagocytic activity and phagocytic index, red blood parameters which are hematocrit and hemoglobin. The survival rate after 5 hr anoxic exposure was also recorded. The best treatments were achieved at a dose of 6 g.kg-1 and 8 g.kg-1in all parameters, except the phagocytic index. The alginate addition at all oxygen stress treatments also performed a better survival rate compared to the control. Adding alginate to feed as feed supplementation by oral administration can boost hematological performance and higher tolerance from oxygen stress exposure.

Pengaruh Tingkat Kerapatan dan Kedewasaan Hutan Mangrove dalam Memerangkap Sedimen di Muara Sungai Langsa Kota Langsa Erlangga Erlangga; Helmi Gusnita; Syahrial Syahrial; Cut Meurah Nur Akla; imamshadiqin imamshadiqin; Riri Ezraneti; Rian Firdaus
Jurnal Kelautan Tropis Vol 25, No 3 (2022): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v25i3.14009

In November 2021, a study on the effect of mangrove density and maturity level on sediment trapping in the Langsa River estuary, Langsa City, was conducted with the objective of determining the density, maturity, and rate of sediment deposition around the mangrove forest, as well as the relationship between mangrove density and maturity level and sediment deposition rate. Mangrove vegetation was collected using a 40 m line transect parallel to the river flow and a 10 x 10 m plot, while sediment samples were collected using a sediment trap. The study's findings indicated that mangrove density varied between 675.00 and 1541.67 ind/ha, that mangrove maturity was uneven, with trunk diameters ranging between 4.14 and 9.83 cm and basal areas ranging between 30.45 and 72.93 m2/ha. Meanwhile, sediment deposition rates ranged between 26.94 and 277.73 mg/cm2/day, with a strong correlation between density and maturity of mangrove vegetation (98% and 99%, respectively). Penelitian terhadap pengaruh tingkat kerapatan dan kedewasaan hutan mangrove dalam memerangkap sedimen di muara Sungai Langsa Kota Langsa dilakukan pada bulan November 2021 dengan tujuan untuk mengetahui tingkat kerapatan, kedewasaan dan laju pengendapan sedimen di sekitar hutan mangrovenya serta untuk mengetahui keterkaitan antara tingkat kerapatan dan kedewasaan hutan mangrove terhadap laju pengendapan sedimennya. Vegetasi mangrove dikumpulkan dengan menarik transek garis sepanjang 40 m yang sejajar dengan aliran sungai dan dibuat plot berukuran 10 x 10 m, sedangkan sampel sedimennya diambil menggunakan sediment trap. Hasil penelitian memperlihatkan bahwa kerapatan mangrovenya berkisar antara 675.00 – 1541.67 ind/ha, kedewasaan mangrovenya tidak merata dengan diameter batangnya berkisar antara 4.14 – 9.83 cm dan basal areanya berkisar antara 30.45 – 72.93 m2/ha. Sementara untuk laju pengendapan sedimennya berkisar antara 26.94 – 277.73 mg/cm2/hari, dimana keterkaitan antara kerapatan dan kedewasaan vegetasi mangrovenya memiliki hubungan yang kuat (masing-masing 98% dan 99%).

Gastropoda Laut dan Chiton di Mikrohabitat Bangunan Pelindung Pantai Pelabuhan Krueng Geukueh Provinsi Aceh Cut M. N. ‘Akla; Fitra Wira Hadinata; Nur Ikhsan; Welmar Olfan Basten Barat; Hendrik V. Ayhuan; Muhammad Hatta; Riri Ezraneti; Syahrial Syahrial
Jurnal Kelautan Tropis Vol 25, No 3 (2022): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v25i3.14950

The study was carried out in March 2022 at 8 observation stations in the Krueng Geukueh Harbor coastal protection facility in Aceh Province, using the visual encounter method for 120 minutes. The Shannon-Weaver diversity index, Simpson dominance, and Shannon-Weaver uniformity were used to generate the gastropod ecology index, while PCA was used to examine dominance by specific species and CA was used to analyze similarities in species composition. The study's findings included 20 species, 7 families, and a group of chitons. Gastropods Littorina angulifera, Echinolittorina puncatata, Nerita chamaeleon, Mauritia arabica, Thais mancinella, Morula musiva and Herba corticata are classified as rare (relative frequency < 20%); Littorina undulata, Nerita planospira, Cypraea mauritiana and Chiton sp. classified as occasional (relative frequency 21 – 40%); Littorina brevicula and Echinolittorina placida are relatively common (relative frequency 41 – 60%); Nerita albicilla, Nerita costata, Tylothais virgata and Tenguella granulata are relatively common (relative frequency 61 – 80%); and Thais (Thalesa) aculeata, Trochus radiatus and Cellana radiata were found to be relatively abundant (relative frequency > 80%). The gastropod diversity and uniformity indices were both low (1.49 and 0.48, respectively), where as the dominance index was moderate (0.52). The PCA analysis revealed that the dominant gastropod species were C. mauritiana, M. arabica, and M. musiva, and the CA analysis revealed the establishment of three groups based on the species composition discovered as a result of human activities near the study site. Kajian dilakukan pada bulan Maret 2022 di 8 stasiun pengamatan pada bangunan pelindung pantai Pelabuhan Krueng Geukueh Provinsi Aceh menggunakan metode visual encounter selama 120 menit. Indeks ekologi gastropoda dihitung dengan indeks keanekaragaman Shannon-Weaver, dominansi Simpson dan keseragaman Shannon-Weaver, sedangkan dominansi oleh spesies tertentu dianalisis menggunakan PCA dan kesamaan komposisi spesiesnya dianalisis menggunakan CA. Hasil kajian mendapatkan 20 spesies dan 7 famili serta ditemukan juga kelompok chiton. Gastropoda Littorina angulifera, Echinolittorina puncatata, Nerita chamaeleon, Mauritia arabica, Thais mancinella, Morula musiva dan Herba corticata tergolong langka ditemukan (frekuensi relatif < 20%); Littorina undulata, Nerita planospira, Cypraea mauritiana dan Chiton sp. tergolong sesekali ditemukan (frekuensi relatif 21–40%); Littorina brevicula dan Echinolittorina placida tergolong sering ditemukan (frekuensi relatif 41–60%); Nerita albicilla, Nerita costata, Tylothais virgata dan Tenguella granulata tergolong umum ditemukan (frekuensi relatif 61–80%); serta Thais (Thalessa) aculeata, Trochus radiatus dan Cellana radiata tergolong melimpah ditemukan (frekuensi relatif >80%). Indeks keanekaragaman dan keseragaman gastropodanya tergolong rendah (1.49 dan 0.48), sedangkan indeks dominansinya tergolong sedang (0.52). Dari analisis PCA didapatkan spesies gastropoda yang mendominasi adalah C. mauritiana, M. arabica dan M. Musiva, kemudian hasil analisis CA memperlihatkan adanya pembentukan tiga kelompok berdasarkan

Korelasi Konsentrasi Mikroplastik dengan Material Padatan Tersuspensi (MPT) di Perairan Delta Sungai Bodri, Kendal, Jawa Tengah Sri Yulina Wulandari; Bambang Yulianto; Ocky Karna Radjasa; Dwi Haryo Ismunarti; Sri Sedjati
Jurnal Kelautan Tropis Vol 25, No 3 (2022): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v25i3.16121

The Bodri river flow in Kendal Regency is a transportation route for sedimentary materials, waste disposal or garbage resulting from human activities on land to sea waters. One type of waste that is often found is plastic packaging. Until now, the use of plastic products is still high in society. In the environment, plastic waste (including that used for packaging) is difficult to decompose or degrade, but only fragmented into smaller sizes called microplastics. Microplastics (particle size < 5 mm) are widely distributed throughout aquatic ecosystems. Microplastics are one of the pollutants that can affect the food cycle for organisms in coastal and marine areas. This study was aimed to determine the relationship between the concentration of microplastics and suspended solids (MPT) at high tide and low tide. A total of 16 water samples were taken from eight (8) purposively determined stations. Sampling was carried out in July 2019 (dry season) during high and low tides. The research method used was descriptive quantitative. The concentration of microplastics was determined by the method recommended by NOAA, while the determination of the concentration of MPT was carried out by the gravimetric method. The results showed that the average concentration of microplastics at high tide was 2.207 mg/L compared to 2.615 mg/L at low tide. The average MPT concentration at high tide is 193 mg/L lower than at low tide, which was 419 mg/L. It could be said that the high mean concentration of microplastic and MPT at low tide indicated that more microplastic and MPT sourced from the mainland. By the statistical test using the Pearson correlation method, the relationship between microplastics and MPT was in the weak category. MPT affected the presence of microplastics only by 3.19% (with a coefficient of determination of 0.0319 and a p-value of 0.508). The presence of microplastics was not only caused by the presence of MPT, but was mostly thought to be influenced by other factors. Aliran sungai Bodri di Kabupaten Kendal menjadi jalur transportasi bahan sedimen, buangan limbah atau sampah hasil dari aktivitas manusia di daratan menuju ke perairan laut. Salah satu jenis buangan sampah yang banyak dijumpai adalah plastik kemasan. Sampai sekarang, penggunaan produk plastik masih tinggi di masyarakat. Di lingkungan, sampah plastik (termasuk yang digunakan sebagai kemasan) sulit terurai, tetapi hanya terfragmentasi menjadi ukuran yang lebih kecil yang disebut mikroplastik. Mikroplastik (ukuran partikel < 5 mm) tersebar luas di seluruh ekosistem perairan. Mikroplastik menjadi salah polutan yang dapat mempengaruhi siklus makanan bagi organisme di wilayah pesisir dan laut. Penelitian ini bertujuan untuk mengetahui hubungan antara konsentrasi mikroplastik dengan material padatan tersuspensi (MPT) pada saat pasang dan surut. Sebanyak 16 sampel air diambil dari 8 stasiun yang ditentukan secara purposif. Pengambilan sampel dilakukan pada bulan Juli 2019 (musim kemarau) saat pasang dan surut. Metode penelitian yang dilakukan adalah deskriptif kuantitatif. Konsentrasi mikroplastik ditentukan dengan metode yang direkomendasikan oleh NOAA, sedangkan penentuan konsentrasi MPT dilakukan dengan metode gravimetri. Hasil penelitian menunjukkan, bahwa rerata konsentrasi mikroplastik saat pasang lebih rendah yaitu sebesar 2,207 mg/L dibandingkan saat surut yaitu sebesar 2,615 mg/L. Rerata konsentrasi MPT saat pasang lebih rendah yaitu sebesar 193 mg/L dibandingkan saat surut yaitu sebesar 419 mg/L. Dapat dikatakan bahwa tingginya nilai rerata konsentrasi mikroplastik dan MPT pada saat surut mengindikasikan kalau mikroplastik dan MPT lebih banyak berasal dari daratan. Dari uji statistik dengan metode korelasi Pearson, hubungan antara mikroplastik dengan MPT termasuk kategori lemah. MPT mempengaruhi keberadaan mikroplastik hanya sebesar 3,19% (dengan koefisien determinasi sebesar 0,0319 dan p-value 0,508). Keberadaan mikroplastik tidak hanya disebabkan oleh adanya MPT, tetapi sebagian besar diduga dipengaruhi oleh faktor-faktor lain.

Komunitas Spons (Porifera) pada Ekosistem Terumbu Karang di Manokwari, Indonesia Nelly Sayori; Tresia S. Tururaja; Duaitd Kolibongso
Jurnal Kelautan Tropis Vol 25, No 3 (2022): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v25i3.14098

Sponges are one of the most influential benthic organisms in coral reef ecosystems. Many studies on sponge communities have been carried out globally, from the tropics to the sub-tropics. However, in Indonesia, the sponge community has not been sufficiently observed, especially its diversity and interactions with habitats. Manokwari, a developing city north of the Bird's Head Seascape region, Papua has a lack of information on benthic communities and no reports of sponges. This study is to examine the sponge community (diversity and distribution) in coral reef ecosystem. This study found that sponge richness (species and morphology) was categorized as “low”, with only 11 species with 8 morphological forms. The most common species included Niphates erecta, Stylissa carteri, and Pseudoceratina purpurea, while the most common growth forms were massive and encrusting, accounting respectively for 27.3% and 18.2% of the total number of species. The highest diversity was found on the island of Kaki (5 species) with the island of Nusmapi having an uneven distribution of sponges. The results of our study found that there was no relationship between sponge diversity and morphology. This baseline information is essential for management of marine biodiversity hotspots in taking decisions for marine life conservation. Spons merupakan salah satu organisme bentik yang paling berpengaruh dalam ekosistem terumbu karang. Banyak penelitian tentang komunitas spons telah dilakukan secara global, dari daerah tropis hingga sub tropis. Namun di Indonesia, komunitas spons belum cukup diamati, terutama keanekaragaman dan interaksinya dengan habitat. Manokwari, kota berkembang di utara dari wilayah Bentang Laut Kepala Burung (BLKB), Papua memiliki kekurangan informasi tentang komunitas bentik dan tidak ada laporan tentang spons. Studi ini untuk mengkaji komunitas spons (keanekaragaman dan distribusi) pada ekosistem terumbu karang. Penelitian ini menemukan kekayaan spons (spesies dan morfologi) yang dikategorikan “rendah”, dengan hanya 11 spesies dengan 8 bentuk morfologi. Spesies yang paling umum termasuk Niphates erecta, Stylissa carteri, dan Pseudoceratina purpurea, sedangkan bentuk pertumbuhan yang paling umum adalah massif dan encrusting dengan menyumbang masing-masing 27,3% dan18,2% dari total jumlah spesies. Keanekaragaman tertinggi ditemukan di pulau Kaki (5 spesies) dengan pulau Nusmapi memiliki sebaran spons tidak merata. Hasil penelitian kami menemukan tidak ada hubungan antara keanekaragaman spons dengan bentuk morfologi. Informasi dasar ini sangat penting untuk pengelolaan hotspot keanekaragaman hayati dalam perumusan keputusan untuk konservasi biota laut.

Dinamika Perubahan Luasan dan Kerapatan Ekosistem Mangrove Di Kawasan Taman Nasional Sembilang Menggunakan Citra Satelit Landsat 8 Sigit Febrianto; Hanan Azzahra Syafina; Nurul Latifah; Max Rudolf Muskananfola
Jurnal Kelautan Tropis Vol 25, No 3 (2022): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v25i3.14909

The Mangrove Ecosystem is the most extensive forest in the Sembilang National Park (TNS) area. This area is located on the east coast of South Sumatra Province. The Sembilang National Park area has a natural mangrove forest of 88,555 ha. This area is known as the largest natural mangrove forest on the island of Sumatra. The purpose of this study was to determine changes in the area and density of mangroves in a period of 5 years using Landsat 8 satellite imagery. The results of the analysis show that the mangrove area in 2014 was 92,731 ha and the mangrove area in 2019 was 88,586 ha, the area decreased by 4,145 ha or about 4.5%. The main factors influencing changes in mangrove area and density are human activities such as land clearing near mangrove forest areas. In addition, there are natural factors that are dominantly influenced by abrasion and river sedimentation. Ekosistem Mangrove merupakan hutan paling luas yang terdapat di kawasan Taman Nasional Sembilang (TNS). Kawasan ini terletak di pesisir timur Provinsi Sumatera Selatan. Kawasan TN Sembilang memiliki hutan mangrove alami seluas 88.555 ha. Kawasan ini dikenal sebagai hutan mangrove alami terluas di Pulau Sumatera. Tujuan penelitian ini adalah untuk mengetahui perubahan luasan dan kerapatan mangrove dalam kurun waktu 5 tahun menggunakan citra satelit Landsat 8. Teknik analisis spasial yang digunakan untuk mengkelaskan mangrove dan non mangrove serta kerapatan dengan menggunakan Supervised Classification dan NDVI. Hasil analisa menunjukkan bahwa luasan mangrove pada tahun 2014 seluas 92.731 ha dan luasan mangrove tahun 2019 seluas 88.586 ha, luasan mengalami penurunan sebesar 4.145 ha atau sekitar 4,5%. Faktor-faktor yang mempengaruhi perubahan luas dan kerapatan mangrove yang utama adalah aktivitas manusia seperti pembukaan lahan di dekat area hutan mangrove. Selain itu, terdapat faktor alam mempunyai yang dominan dipengaruhi oleh abrasi dan sedimentasi sungai.

Fraksinasi Flavonoid Spirulina platensis dengan Metode Kromatografi Lapis Tipis dan Aktivitas Inhibisi Enzim α-Glukosidase Hartoyo Notonegoro; Heder Djamaludin; Iriani Setyaningsih; Kustiariyah Tarman
Jurnal Kelautan Tropis Vol 25, No 3 (2022): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v25i3.13905

Spirulina platensis is a type of Cyanobacterium microalgae that forms multicellular helicoidal filaments. Spirulina platensis contains primary and secondary metabolites. The type and amount of the active compound Spirulina platensis depends on the method of extraction, fractionation, and isolation. So far, there are not much research data related to the active compound of Spirulina platensis extract cultured on Walne. The purpose of this study was to use of thin layer chromatography (TLC) method to separate flavonoids from Spirulina platensis biomass extract cultured on Walne media and the activity of a-glucosidase enzyme inhibition using biomass, crude extract, active fraction of flavonoids and phycocyanins from Spirulina platensis. This research method is descriptive experimental, which the Spirulina platensis is cultured on 80 g/L NaNO3 modified Walne media, extracted by maceration, fractionated by TLC and isolated the active compound by Preparative TLC (PTLC). The analysis included fraction and isolation of flavonoids from Spirulina platensis. The results showed that the TLC could be used to identify the active compound of Spirulina platensis extract cultured on Walne. Fractionation of Spirulina platensis extract using stationary phase silica gel Si 60 GF254 and the best mobile phase with a combination of chloroform:ethyl acetate (6:4) and an Rf1 value of 0.58; Rf2 0.71; and Rf3 0.83, as well as yellow-orange spots. Isolation of the active compound of Spirulina platensis extract using PLTC stationary phase silica gel Si 60 PF254 and the best mobile phase combination eluent chloroform:ethyl acetate (9:1). RF value of Rf2 0.57; Rf3 0.86; and Rf4 0.93 with dark yellow-brown spots. The color of the spots from the PLTC results shows that the active compounds of Spirulina platensis extract are flavonoid compounds. Biomass, crude extract, phycocyanine extract and flavonoids from Spirulina do not have inhibitory activity against α-glucosidase enzyme. Spirulina platensis merupakan jenis mikroalga Cyanobacterium yang membentuk filamen helicoidal multiseluler. Spirulina platensis mengandung senyawa metabolit primer dan sekunder. Jenis dan jumlah senyawa aktif Spirulina platensis tergantung pada metode ekstraksi, fraksinasi dan isolasi. Sejauh ini belum banyak data hasil penelitian terkait senyawa aktif ekstrak biomassa Spirulina platensis yang dikultur pada media Walne. Tujuan penelitian ini yaitu penggunaan metode kromatografi lapis tipis (KLT) untuk memisahkan flavonoid ekstrak biomassa Spirulina platensis yang dikultur pada media Walne serta aktivitas inhibisi enzim α-glukosidase menggunakan biomassa, ekstrak kasar, fraksi aktif flavonoid dan fikosianin dari Spirulina platensis. Metode penelitian ini adalah eksprimental deksriptif dimana mikroalga Spirulina platensis dikultur pada media Walne modifikasi 80 g/L NaNO3, diekstraksi dengan maserasi, difraksinasi dengan KLT dan diisolasi senyawa aktif dengan KLT Preparatif (KLTP). Analisis yang dilakukan meliputi analisis fraksi dan isolasi flavonoid dari Spirulina platensis. Hasil penelitian menunjukkan metode KLT dapat digunakan untuk mengindetifikasi senyawa aktif ekstrak Spirulina platensis yang dikultur pada media Walne. Fraksinasi ekstrak Spirulina platensis menggunakan fase diam silika gel Si 60 GF254 dan fase gerak kombinasi eluen terbaik kloroform:etil asetat (6:4) dan nilai Rf1 0,58; Rf2 0,71; dan Rf3 0,83, serta bercak berwarna kuning-oranye. Isolasi senyawa aktif ekstrak Spirulina platensis menggunakan KLTP fase diam silika gel Si 60 PF254 dan fase gerak kombinasi eluen terbaik kloroform:etil asetat (9:1). Nilai Rf2 0,57; Rf3 0,86; dan Rf4 0,93 dengan bercak berwarna kuning-cokelat gelap. Warna bercak hasil KLTP menunjukkan komponen senyawa aktif ekstrak Spirulina platensis berupa senyawa golongan flavonoid. Biomassa, ekstrak kasar, ekstrak fikosianin dan flavonoid dari Spirulina tidak memiliki aktivitas penghambatan terhadap enzim α-glukosidase.

Logam Berat Mercury (Hg) dan Arsen (As) pada Hasil Tangkapan Nelayan Pesisir Semarang dan Tegal Jawa Tengah Chrisna Adhi Suryono; Agus Indardjo
Jurnal Kelautan Tropis Vol 25, No 3 (2022): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v25i3.16279

The Semarang and Tegal coastal waters are traditional fishing ground areas. However, the increasing use of coastal areas for various activities will increase pollutants in the coastal waters. Heavy metals are pollutants that always appear in waters and accumulate in marine organisms. These study aims to determine As and Hg metals contained in the tissues of several marine organisms caught on the coast of Semarang and Tegal, Central Java. The analysis of As and Hg concentrations in organismal tissues using ICPMS. The results showed that As and Hg were found in biota such as white shrimp (P. merguensis), blue crab (P. pelagicus), shellfish (A. inaequivalvis) and gastropods (H. ternatanus). Arsenic concentrations in the four biotas caught in Tegal (1.6 – 5.3 ppm) and Semarang (2.58 – 4.9 ppm). Meanwhile, Hg metal (0.02 – 0.15 ppm) in Tegal and (0.034 – 0.15 ppm) in Semarang. The concentration level of As based on the biota caught in the Tegal waters are sequentially Shellfish > White shrimp > Gastropods > Blue swimming crab. Meanwhile, the concentration of Arsenic in the biotas in Semarang waters are sequentially shellfish > white shrimp > blue crab > gastropods. However, Hg metal in the biota obtained in these two areas is very low Pesisir Semarang dan Tegal merupakan daerah penangkapan perikanan tradisional. Namun dengan semakin meningkatnya pemamnfaatan wilayah pesisir untuk berbagai kegiatan akan meningkatkan polutan dalam perairan. Logam berat merupakan polutan yang selalu muncul dalam perairan dan terakumulasi organisme laut. Penelitian ini bertujuan untuk mengetahui logam As dan Hg yang terdapat dalam jaringan beberapa biota yang ditangkap pesisir Semarang dan Tegal Jawa Tengah. Analisa konsentrasi As dan Hg dalam jaringan organisme menguunakan ICPMS. Hasil penelitian menunjukan logam As dan Hg ditemukan dalam biota seperti udang putih (P. Merguensis), rajungan (P. pelagicus), kerang bulu (A. inaequivalvis) dan gastropoda (H. ternatanus). Konsentrasi Arsen di keempat biota yang ditangkap di Tegal (1,6 – 5,3 ppm) dan Semarang (2,58 – 4,9 ppm). Sedangkan logam Hg (0,02 – 0,15 ppm) di Tegal dan (0,034 – 0,15 ppm) Semarang. Tingkat konsentrasi logam As berdasarkan biota yang tertangkap di perairan Tegal secara berurutan Kerang bulu > Udang putih > Gastropoda > Rajungan. Sedangkan konsentrasi Arsen dalam biota di perairan Semarang secara berurutan Kerang bulu > Udang putih > Rajungan > Gastropoda. Namun logam Hg dalam biota yang didapat di kedua daerah tersebut sangat rendah.

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kota semarang,

Jawa tengah

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name -

Contact Email -

Phone -

Journal Mail Official -

Editorial Address -

Location Kota semarang, Jawa tengah INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kota semarang,

Jawa tengah

INDONESIA