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INDONESIA
Journal of Tropical Biodiversity and Biotechnology
Published by Universitas Gadjah Mada
ISSN : 25409573     EISSN : 25409581     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
Journal of Tropical Biodiversity and Biotechnology (JTBB) is an authoritative source of information concerned with the advancement of tropical biology studies in the Southeast Asia Region. It publishes original scientific work related to a diverse range of fields in tropical biodiversity, functional biology, and biotechnology.
Arjuna Subject : -
Articles 327 Documents
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Effectivity of Spodoptera littoralis Nucleopolyhedrovirus (SpliMNPV) and Natural Additives Mixtures against Spodoptera litura Fab. (Lepidoptera: Noctuidae) on Cabbage Plants Sukirno Sukirno; Bellia Alda Ayu Prasetya; Aryo Seto Pandu; Siti Sumarmi; Hari Purwanto; Ignatius Sudaryadi; Suparmin Suparmin; RC Hidayat Soesilohadi
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 2 (2022): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.71134

Abstract

Armyworm (Spodoptera litura Fab.) is one of the agricultural pests that can cause huge losses especially for Indonesian farmers because it is damaging various crops, especially cabbage (Brassica oleifera L.). Spodoptera littoralis nucleopolyhedrovirus (SpliMNPV) is one of the biological agent which is effective for the management of the Spodoptera litura. However, because of UV radiation it easily degraded when applied in the fields. This study was aimed to determine the effectivity of several indigenous plants for UV protectant of SpliMNPV for controlling armyworm at greenhouse scale. Extracts of 2% (w/v) of turmeric rhizome, red betel leaf, moringa leaf, and clove flower, were formulated with SpliMNPV and sprayed evenly onto two-month- old cabbages. The experiment used five replicates with six periods of sunlight exposures (0, 1, 3, 5, 7, and 15 days). A commercial product of deltamethrin was used as a comparison. The sprayed leaves were then used as a bioassay by using 25 individuals of one day old 1st larval instar by five replicates. The results showed that the turmeric additive was the most effective as a UV protectant and effectively prolonged the half-life of SpliMNPV to 4.12 days, while for clove, moringa leaf, and red betel was 2.48, 2.15, and 2.28 days, respectively.
Java Sparrow Lonchura oryzivora at Bali Barat National Park: Do They Still Persist? Luh Putu Eswaryanti Kusuma Yuni; I Made Saka Wijaya; Mas Untung; Gde Oka Widiyavedanta; Egmont Congdenjit; Pramana Yuda
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 2 (2022): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.71503

Abstract

The main purpose of the establishment Bali Barat National Park was to conserve the endemic endangered Bali Starling. However, based on data on 2004, one endangered species, Java Sparrow Lonchura oryzivora also resided in there. Current official report of sighting is ultimately required since it acts as reference in the management of a conservation area. We reported four sightings of Java Sparrow flocks occurred in June, 2021 at the Prapat Agung Peninsula, 28 individuals in total consisted of 13 adults and 15 juveniles, in a transition area of monsoon forest and savannah. The biggest flock sighted was eleven individuals. This study therefore confirmed that the Java Sparrow was still persisted at the Bali Barat National Park in 2021, in Prapat Agung Peninsula in particular. 
Leaf Morphometric and Chlorophyll Content Study of Bisbul (Diospyros discolor Willd.) at the Bogor Botanical Garden Irvan Fadli Wanda; Arief Noor Rachmadiyanto; Ade Ayu Oksari
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 2 (2022): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.72565

Abstract

Bisbul (Diospyros discolor Willd.) is one of the collections from the Bogor Botanical (BBG). The wide distribution of this plant in Southeast Asia's tropical forests creates many morphometric variations of this species. The diversity of leaf morphometric variations of a plant species can be the basis for grouping various species. This study aimed to determine the morphometric diversity of Bisbul leaves from various locations of origin. The study took samples of bisbul leaves from Bogor Botanical Gardens from three accessions, namely the Philippines, West Java, and Papua. All the trees are over 30 years old. The leaves are taken based on a horizontal position, vertical crown, and leafage. The results showed that the origin of the location gave different multiplier values to calculate the area, namely the Philippines y = 0.733x + 0.034; West Java y = 0.765x-2,949; and Papua y = 0.758x-1.389. The length to leaf width ratio also has differences, namely, the Philippines, which is 2.64, West Java 2.65, and Papua 2.81. The chlorophyll content in young leaves increases in old leaves. The samples from Papua also have the highest chlorophyll content compared to the Philippines and West Java. This difference indicates morphometric variations between the three, even within one species. Some environmental conditions may affect shade areas and tree age. In addition, DNA research from accession D. discolor is also needed to determine the cause of the morphometric variation. 
Analysis of Soil Bacterial Diversity from Tropical Rainforest and Oil Palm Plantation In Jambi, Indonesia by 16S rRNA-DGGE Profiles Risky Hadi wibowo; Nisa Rachmania Mubarik; Iman Rusmana; Maggy Thenawidjaya Suhartono; Sipriyadi Sipriyadi; Masrukhin Masrukhin
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 2 (2022): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.68820

Abstract

Oil palm plantations are the most invasive land use changes in Southeast Asia. It must have affected unique natural biodiversity. This study aimed to investigate the diversity of soil bacteria based on 16S rRNA gene profiles from tropical forest and oil palm plantation in Jambi Province, Indonesia. Soil sample was taken from tropical forest and oil palm plantation from Jambi province, Indonesia. The forest site is in Bukit Duabelas National Park, and the nearby oil palm plantation is in Sarolangun District, Jambi Province, Indonesia. The diversity of bacterial communities from topsoil was studied using Denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene and common biodiversity indices. PCR amplification of 16S rRNA gene was successfully conducted primers-using 33F/ 518R primers. Phylogenetic approach was used for revealing the community shift of bacterial phyla and genera in both areas. Phylogenetic analysis showed there were 4 phyla of bacteria i.e., Firmicutes, Alphaproteobacteria, Gammaproteobacteria, and Actinobacteria, respectively. Actinobacteria was the most dominant group in both areas. The composition of soil bacterial community in the oil palm plantation, based on total number of bands 16S rRNA generated from DGGE was richer than that in the Bukit Dua Belas National Park. It was probably caused by plantation year circle more than 10 years and routine activities during the plantation management, such as applications of agricultural lime, herbicide and fertilizer. 
The Effect of Liquid Organic Fertilizer “Bio Ferti” Application on the Growth Rate of Spirulina platensis by Using Haldane Model M Iqbal Maulana Ginting; Eko Agus Suyono; Mochammad Donny Koerniawan; Lucia Tri Suwanti; Ulfah Juniarti Siregar; Arief Budiman
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 2 (2022): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.68944

Abstract

This experimental research was performed to observe the influence of an agricultural liquid organic fertilizer called Bio Ferti on the growth and biomass of Spirulina platensis, aiming at replacing inorganic fertilizer with the liquid organic one. The cultivation of the microalgae was conducted over seven days at Nogotirto Algae Park. The liquid organic fertilizer, namely Bio Ferti, was obtained from the Faculty of Biology, Universitas Gadjah Mada, and prepared to have doses of 2, 4, 6, 8, and 10 mL. For comparison, an inorganic fertilizer with the same doses was also prepared. The variables to be observed were cell density, dry cell weight, and growth kinetics. The culture medium conditions observed were temperature, pH, and salinity (the optimum salinity was 20 ppt). The growth kinetic analysis was performed mathematically using numerical simulations using the Contois and the Haldane models. This research’s results showed that Bio Ferti affected the growth rate of Spirulina platensis. With a dose of 2 mL, it became the optimum medium which produced the highest density and dry weight of 1.78x106 cells/mL and 160 mg/mL, respectively. Meanwhile, the inorganic fertilizer with a dose of 10 mL produced the highest density and dry weight of 2,13x105 and 80 mg/mL, respectively. The temperature ranged from 28 to 31°C, while the pH ranged from 8.01 to 9.02 for each medium. The suitable model to describe the growth kinetics of Spirulina platensis was the Haldane model.
Rediscovery of Bombus rufipes Lepeletier 1835 (Hymenoptera: Apoidea: Bombidae) on Mount Slamet Imam Widhiono; Trisno Haryanto; Eming Sudiana; Elly Proklamasiningsih; Edy Yani
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 2 (2022): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.70620

Abstract

Bombus rufipes Lepeletier 1835 (Hymenoptera: Bombidae) is the only species of Bombidae found in Java. Recent information suggests that it occurs in Java on Mounts Salak 1200 m asl., Mt. Halimun, Mt. Pangrango Gede Complexes, Mt.Cermai, (West Java) Mt.Slamet , Mt. Merapi, Mt. Merbabu, Mt Telomoyo (Central Java) and Mt Argopuro (East Java) , at altitudes above 1,500 m asl. We sought to rediscover this species on Mount Slamet by surveying natural forests at altitudes of 1,500–2,500 m asl on the eastern slopes of Mount Slamet, from August to October 2020 and August to October 2021. Descriptive and morphometric data were obtained and analyzed. The survey revealed five colonies nesting on the ground at an average depth of ca. 70 cm; the colonies contained 18–24 individuals and 22–36 cells (brood, honey, and pollen cells). Based on morphometric measurements and the description of Frison (1930), the species was identified as Bombus rufipes Lepeletier 1835. Therefore, Bombus rufipes still occurs on Mount Slamet. These results provide basic information that should aid further research on this species. 
Bycatch of Amboina Box Turtle (Cuora amboinensis) by Fishermen in Rawa Aopa, Southeast Sulawesi Harnum Nurazizah; Mirza Dikari Kusrini; Ani Mardiastuti
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 2 (2022): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.72113

Abstract

Rawa Aopa is a permanent swamp ecosystem that serves as one of the suitable habitats for amboina box turtles (Cuora amboinensis). Some of the area is part of a national park (Rawa Aopa Watumohai National Park) and is designated as a traditional zone for fishing activities, where local fishermen sometimes reported accidental catch C. amboinensis in their fishing gear. The aims of this study were to record the existence of C. amboinensis bycatch, size and age structure of bycatch, characteristic of fishing activity, and to discuss the conservation implication of bycatch.  The number of bycatch was recorded by direct observation of 7 selected fishermen in 14 days. The C. amboinensis caught accidentally were measured and weighed. A total of 38 individuals of C. amboinensis were accidentally caught by fishermen during the study, having a size ranged of 7.4 to 18.5 cm (juveniles, young adults, and old adults; no hatchling), and weighed 248 to 996 g. Based on sex, there was no significant difference between morphological size of male and female, although bycatch for females (59%) tend to be slightly higher than males (41%). Most of fishermen lives in Pewutaa Village and used bubu traps to catch fishes. C. amboinensis are the most common bycatch compared to other species. In order to minimize the impact of bycatch of the C. amboinensis by fishermen, we need to ensure that the turtle that accidentally trapped in the fishermen’s fishing gear would be released unharmedly to their habitat. 
Morphometric Analysis of Sumatran, Kalimantan, and Javan Cyrtodactylus, which were Labelled as Cyrtodactylus marmoratus, Revealed Undescribed Species Muhammad Alif Fauzi; Awal Riyanto; Nia Kurniawan
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 3 (2022): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.66688

Abstract

Cyrtodactylus marmoratus is originally described based on the specimen from Java. Due to the marbled body colour pattern and the similarity in morphology, many specimens from outside of Java have been identified as C. marmoratus. The ongoing research, both molecular and morphological studies, showed that C. marmoratus is restricted to Java. The taxonomic status of specimens labelled as C. marmoratus from outside Java remains unresolved. Here, we study the morphometric and meristic of Javan, Sumatran, and Kalimantan Cyrtodactylus which were labelled as C. marmoratus to reveal their taxonomic status. We examined 11 morphometric and 19 meristic characters in 51 specimens at Museum Zoologicum Bogoriense (MZB) originally from, Java, Sumatra, and Kalimantan labelled as C. marmoratus as well as other recognized species. Principal Component Analysis (PCA) results show that C. marmoratus from Java differs from previous specimens which labelled as C. marmoratus from Sumatra and Kalimantan. The PCA results also show that C. marmoratus from Martabe differs from C. marmoratus Java which is supported by statistical analysis on interorbital, HeadW, HeadD, tubercle on the ventrolateral fold, dorsal tubercle and ventral scales. We hypothesized that Cyrtodactylus from Martabe is a suspected undescribed species-await formal description, and overall molecular analyses are needed for future study. 
Immunonutrition and Hepatoprotectant Aspects of Moringa Oleifera Leaf Nanoemulsion Syrup as an Antituberculosis Adjuvant for Children with Tuberculosis Nyoman Budhi Wirananda Setiawan; Agus Indra Yudhistira Diva Putra; Made Indira Dianti Sanjiwani; Agung Wiwiek Indrayani; Ida Ayu Ika Wahyuniari
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 3 (2022): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.68291

Abstract

Tuberculosis in children is a global health problem that decreases the quality of life of children. Based on data from the Indonesian Ministry of Health in 2016, nearly 69.000 children had tuberculosis and the case keeps increasing every year. Moringa oleifera leaf nanoemulsion syrup has immunonutrition and hepatoprotectant effects in children with tuberculosis. Moringa oleifera leaf nanoemulsion syrup contains proteins, micronutrients, and minerals which have a biological role as an immunity agent and prevent toxic effects of tuberculosis drugs. Until now, the use of Moringa oleifera leaf nanoemulsion syrup has been carried out for the immunomodulatory and hepatoprotective aspects. Immunomodulatory and hepatoprotective aspects will be discussed further in this literature review. The sources of articles in this literature review are pubmed.com, ncbi.com, plosone.com, sciencedirect.com, and googleschoolar.com from 2010-2020, except when there is no new research against the article. The authors searched for the keywords: "immunonutrition", "tuberculosis in children", "hepatoprotectant", and "Moringa oleifera". As an immunomodulator, Moringa oleifera leaf nanoemulsion syrup stimulate activation of polimorphonuclear (PMN) cells. As a hepatoprotectant, Moringa oleifera leaf nanoemulsion syrup work by reducing the side effects of conventional tuberculosis drugs such as rifampicin by suppressing the action of cytochrome p450 (CYP1A2 and CYP2B), thus decreases the production of toxic hydrazine which causes liver toxicity in tuberculosis patient. Seeing the various interests in the immunomodulatory and hepatoprotective aspects, Moringa oleifera leaf nanoemulsion syrup can be used as an adjuvant therapy in overcoming tuberculosis in children by stimulating the activation of immunity cell such as PMN, increasing nutrient absorption, and suppressing the action of cytochrome p450 (CYP1A2 and CYP2B).
The Effectiveness of Stingless Bees on Pollination of Bitter Melon Plants Momordica charantia L. (Cucurbitaceae) Andi Gita Maulidyah Indraswari Suhri; RC Hidayat Soesilohadi; Ramadhani Eka Putra; Rika Raffiudin; Hery Purnobasuki; Ali Agus; Sih Kahono
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 3 (2022): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.69124

Abstract

This study aimed to measure the effectiveness of stingless bee Tetragonula cf. biroi pollination on the fruit formation of bitter melon Momordica charantia plants. We used hoods on the observed bitter melon plants. In the first hood, stingless bees are inserted to help pollinate 100 bitter melon plants, while in the other hoods, stingless bees are not inserted so that there is no assistance in pollinating the other 100 bitter melon plants. The method used is the focal sampling method for 25 days of observation. Based on the results of the study, stingless bee pollination assistance increased the percentage of the number of flowers that became fruit by 390%, the weight of seeds/fruit by 64%, number of seeds/fruit by 260%, fruit weight by 163%, fruit diameter by 91%, and fruit length by 86%. In addition to the size of the fruit, the shape of the bitter melon pollinated by bees is standard (long and straight). In contrast, the bitter melon that does not get pollination assistance grows with a bent shape resembling the letter "C." Bitter melon is an agricultural commodity that needs pollinating agents such as stingless bees because of its monoecy. 

Page 17 of 33 | Total Record : 327

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