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Journal of Tropical Biodiversity and Biotechnology
ISSN : 25409573     EISSN : 25409581     DOI : -
Journal of Tropical Biodiversity and Biotechnology (JTBB) is an authoritative source of information concerned with the advancement of tropical biology studies in the Southeast Asia Region. It publishes original scientific work related to a diverse range of fields in tropical biodiversity, functional biology, and biotechnology.
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Articles 327 Documents
Astaxanthin Production from Green Microalga Haematococcus pluvialis under Various Bean Sprout Media Concentrations and Duration of UV Radiations Hary, Biaggi Rakhmat Rheinan; Sidharta, Boy Rahardjo; Arsiningtyas, Ines Septi
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.73763

Abstract

Astaxanthin (AX) is known as a very strong antioxidant and has been utilised in many kinds of products such as foods, pharmaceutical, cosmetics, aquaculture, etc. One of the natural resources of AX is Haematococcus pluvialis which has been investigated by some researchers in order to enhance the AX production. However, the production of AX from the microalgae is still costly, hence, this present research is proposing low-cost methods namely bean sprout media (BSM) as an alternative growth media and UV radiation. The variations of BSM concentrations (2, 4, and 6 %) and times of UV radiation (1.5 and 3 hrs) were treated to H. pluvialis in laboratory conditions. BSM 4 % treatment showed an optimum growth of the microalga at 427 x 104 cell/ml (day 8) which also exhibited macrozooid, palmella, and aplanosore phases. UV radiation for 3 hr revealed that the concentration of AX production was as much as 17.37±0.04 mg/l. The research results were potential to be developed further in order to discover better and cheaper methods for scaling up AX production. 
Plant Growth Promoting Endophytic Microorganisms from Orchids for A Sustainable Agriculture Goh, Lucky Poh Wah; Jaisi, Benardette Lyovine; Jawan, Roslina; Gansau, Jualang Azlan
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.74403

Abstract

Conventional agriculture practice has heavily relied on chemical fertilizers to increase crop yield. However, long-term application of chemical fertilizers carries tremendous negative impact on the environment and is unsustainable. Hence, the search for an alternative source of fertilizers is required. Orchids are flowers and can be found in tropical countries. The growth and development of orchids are closely tied to the presence of plant growth promoting endophytic microorganisms (PGPM). PGPM harbours various beneficial traits such as potassium and phosphorus solubilization and indole acetic acid and siderophore production which enhance and support plant growth and development. This review article showed that PGPM isolated from orchids could be utilized in conventional agriculture to reduce dependency on chemical fertilizer. 
Extremophilic Cellulases: A Comprehensive Review Mohanta, Subham; Bahuguna, Megha; Baley, John David; Sharma, Shivika; Sharma, Vikas
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 3 (2023): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.74986

Abstract

Microbial cellulases are an important industrial enzyme having diverse applications in biotechnology, environmental challenges, industrial products and processes. Extremophiles like thermophillic bacteria are a good source of industrially important cellulases as these can withstand industrially rigorous procedures like paper deinking, fabric material softening, bio stoning, paper and pulp, biopolishing cloth material, animal feed and juice. Identification of novel cellulases or improving them through biotechnological interventions has remained a challenge for researchers. Genetic manipulation of thermophilic bacteria for increased cellulase production or synthetic biology approaches for cellulase gene/gene cluster extraction from thermophilic bacteria and expression in appropriate hosts for improved cellulase synthesis. The classic and high-throughput technologies like genomics, metagenomics and bioinformatics could be exploited to isolate cellulase genes from a variety of thermophilic bacteria and further processing. Keeping in view the ultimate requirement of extremophilic cellulases in industries, present study is a compilation of various aspects related to extremophilic cellulases their sources, production, biotechnological interventions and challenges.  
Maturation of Female Yellow Rasbora (Rasbora Lateristriata Bleeker, 1854) Using Oodev at Different Doses in Feed Rey, Juniman; Widiyanto, Slamet; Retnoaji, Bambang
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 3 (2023): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.75916

Abstract

The current high demand for Yellow rasbora (Rasbora lateristriata) is not supported by the availability of captured Yellow rasbora in nature. Aquaculture is the most rational way of utilizing biological natural resources. In intensive aquaculture, it is necessary to optimize all processes that occur in aquaculture, including hatchery. However, the common problem that often happens in hatchery activities is spawning which depends on the season. The hormonal manipulation technique is an appropriate way to stimulate gonadal maturation. Oodev is a hormonal combination of pregnant mare serum gonadotropin and anti-dopamine to stimulate gonadal maturation. The purpose of this study was to determine the effectiveness of using the Oodev with different doses in feed to accelerate gonad maturation of female Yellow rasbora. The study was carried out with four treatments and three replications in 21 days with different doses of Oodev, such as; A (Feed without Oodev), B (0.5 mL/kg feed), C (1 mL/kg feed) and D (2 mL/kg feed). The parameters observed in this study were gonad maturity level, histological structure of ovary, gonadosomatic index, fecundity, and diameter of eggs. The results showed that the dose of Oodev at 1.0 mL/kg feed was an effective dose to optimize the gonad maturity of female Yellow rasbora. This is proven by the highest results shown on all parameters, such as; the maturity level in the IV phase, histological structure of the ovary which showed the dominance of the oocyte maturation phase, gonadosomatic index of 14.014%, the fecundity of 721 eggs, and egg diameter of 0.865 mm. In conclusion, using Oodev in feed at a dose of 1.0 ml/kg of feed for 21 days is an effective dose to optimize the maturation of female Yellow rasbora. 
Scanning Electron Microscopy Analysis of Tea’s Embryo Axis Explant Cultured on Murashige and Skoog Medium Containing 2,4-Dichlorophenoxyacetic acid Eskundari, Ratna Dewi; Taryono, Taryono; Indradewa, Didik; Purwestri, Yekti Asih
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.76451

Abstract

Camellia sinensis L. is an important crop in Indonesia as healthy beverage that contains several secondary metabolism compounds, such as polyphenols and catechins. Tissue culture including somatic embryogenesis and organogenesis has been used for propagating plant for various needs. In this present short-communication, scanning electron microscopic (SEM) analysis of tea was conducted and discussed. This study aimed to investigate surface ultrastructure of TRI2025 embryo axis tea clone cultured on Murashige and Skoog (MS) medium containing 2,4-Dichlorophenoxyacetic acid (2,4-D). The results revealed two different forms of explant’s development, i.e. somatic embryo and transitional form between somatic embryogenesis and organogenesis; or called by “Globular-like Structure” (GLS). Surface ultrastructure analysis of somatic embryo and GLS revealed respectively many stages of somatic embryo development i.e. globular, torpedo, and cotyledon stage, and leaf development form GLS regeneration. 
Spatial Modelling Habitat Suitability of Javan Langur (Trachypithecus auratus É. Geoffroy Saint-Hilaire, 1812) in Bromo Tengger Semeru National Park (TNBTS), East Java Ningtyas, Ari Nadya; Aryanti, Nirmala Ayu; Susilo, Tander Scila Serata Dwi; Rahmadana, Mahmuddin; Agustin, Ika Yuni
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.76841

Abstract

Javan Langur (T. auratus) is well-known as one of endemic primates from Java, Bali and Lombok Islands. The activities of land clearing, vegetation conversing, wild hunting and illegal wildlife trading are the main causes of the extinction of the Javan Langur. It can be used as an important issue for conservation action by making prediction maps of suitable habitat potential, especially for species facing a high risk of extinction in the wild.  We were documenting an information about potential habitat for Javan langur using spatial suitability model in order to provide rigorous information as the basis for conservation activities of Javan langur in TNBTS. We used Landsat-8 TM image and geospatial data to support analysis as a representative of environmental parameters in order to develop the habitat model. We were using maximum entropy (MaxEnt) algorithm refers to Javan langur presence or absence. The results showed that the suitability of the Javan langur habitat in TNBTS has an excellent model accuracy level with an AUC (Area Under the Receiver Operating Characteristics) value of 0.964 and a standard deviation of 0.961. Parameters with the highest response values here are elevation, NDVI (Normalised Difference Vegetation Index) and temperatures. 
The Growth Response of Rendeu (Staurogyne elongata (Neese) Kuntze) to Shoot Pruning and Its Propagation by Shoot Cutting Lailaty, Intani Quarta; Astutik, Sri; Surya, Muhammad Imam
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.77078

Abstract

Rendeu (Staurogyne elongata (Neese) Kuntze) is a native Indonesian plant used as food and traditional medicine in the daily life of the people residing around Gunung Halimun-Salak National Park. Due to the potential source of herbal-based medicines and traditional food in the long-run purposes, the proper method of its propagation is required, so that Rendeu can be conserved and utilised sustainably. This study employed two research designs. First, a completely randomized design with pruning and IAA (indole-3 acetic acid) treatment was used for seedling growths. Second, plant propagation applied a factorial randomized block design: planting media types and plant growth regulator (PGR) (rootone F) treatment. Observation included the number of buds, number of leaves, number of flowers, plant biomass, root length, and relative chlorophyll content using the SPAD tool. The data were analysed using ANOVA (SPSS ver. 17.0), followed by Pearson correlation analysis. The results showed that applying IAA and leaf pruning could increase the number of buds, the number of leaves and the fresh weight of S. elongata plants compared to the control plant. The addition of rootone increased the growth of Rendeu shoot cuttings, shown in all growth parameters and chlorophyll content. Humus was the best media for Rendeu’s growth among all planting medium.  Planting media affected the increase in the number of leaves and the number of buds of S. elongata significantly. The interaction of planting media and PGR somewhat influenced root length and total leaf chlorophyll. The growth and production of S. elongata increased with the time of planting. 
Identifying Single Nucleotide Polymorphisms (SNPs) in OsFER1 and OsFER2 Genes Linked to Iron accumulation in Pigmented Indonesian Rice (Oryza sativa L.) Pratiwi, Apriliana; Bassalamah, Rizka Fahma; Elvani, I Sabila; Sebastian, Alfino; Purwestri, Yekti Asih
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.78019

Abstract

Iron (Fe) is an essential micronutrient for the well-being of plants, animals, and bacteria. In plants, iron plays a pivotal role in a myriad of metabolic processes, encompassing redox reaction, photosynthesis, respiration, chlorophyll synthesis, and nitrogen fixation. For humans, iron is indespensable for several metabolic functions, particularly in the synthesis of haemoglobin. Iron deficiency can lead to health issues on a global scale, therefore identifying key crops, such as rice for providing sufficient iron in diet intake is very important. In rice, the maintenance of iron homeostasis is orchestrated by various genes, with OsFER1 and OsFER2 acting as iron accumulator genes in leaves, stems, flowers, and grains. The primary objective of this study was to ascertain the single nucleotide polymorphisms (SNP) in the OsFER1 and OsFER2 and to assess the iron content in Indonesian local rice cultivars. To achieve this, we examined partial sequences of OsFER1 and OsFER2 to identify SNPs in the Indonesian rice cultivars used (Cempo Ireng, Pari Ireng, Hitam Kalsel, Merah Pari Eja, and Ciherang). Concurrently, the iron content in the seeds was quantified using Atomic Absorption Spectrophotometry (AAS). The analysis revealed that the OsFER1 gene sequence, specifically exon 5, exhibited a SNP in the form of a transition. In contrast, the OsFER2 gene sequences, specifically in intron 2 displayed SNPs in the form of insertions. Notably, the iron content in the seeds was highest in Cempo Ireng (black rice), while it was lowest in Merah Pari Eja (red rice) and Ciherang (non-pigmented rice). Importantly, the identified SNPs in these partial gene sequences did not exert any discernible influence on iron levels or the formation of ferritin protein. 
The Diversity of Scarabaeid Beetles (Scarabaeidae: Coleoptera) in The Lowland Rainforest Ecosystem of Sorong Nature Tourism Park, West Papua, Indonesia Fitradiansyah, La Ode; Atmowidi, Tri; Priawandiputra, Windra; Kahono, Sih
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 3 (2023): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.78230

Abstract

Scarabaeid beetles have an essential role in forest ecosystems, such as nutrient recycling, seed dispersal, forest regeneration, controlling parasite, and reducing carbon emissions. Until now, there has been no publication on scarabaeid beetle diversity in the lowland rainforest ecosystem of Papua, Indonesia. This study aims to measure the diversity of scarabaeid beetles in the lowland rainforest ecosystem of Sorong Nature Tourisme Park (SNTP), West Papua, Indonesia. Determination of study sites used in this study was using the purposive sampling method in three habitat types i.e., rehabilitation zone, conservation zone, and protection zone using baited dung traps (type A, B, and C dung traps), light trap, and active sampling. The fresh cow and human feces baits were used for dung traps that was replaced every 24 hours (68 repetitions for 68 days) in each habitat. Results showed a total of 30 individuals belonging to 13 species of scarabaeid beetles were collected. Onthophagus has the highest species richness (5 species) and the low species richness were Aphodius sp., Anomala sp., and Adoretus sp. (1 species). The protection zone has the highest diversity index (H’=2.09), followed by the conservation zone (H’=2), and rehabilitation zone (H’=0.5). Based on trap type, dung trap collected the most beetle species (9 species), followed by light trap (6 species), and active sampling (2 species). Based on Pearson correlation, soil pH significantly correlated with beetle abundance. This study was the first report of scarabaeid beetles in the West Papua, Indonesia.
Bioactivity and Metabolites Compounds of Medicinal Plants Endophytic Fungi in Indonesia Sukmawaty, Eka; Karim, Abdul; dwyana, Zarsawaty; Natsir, Hasnah; Karim, Harningsih; Ahmad, Ahyar
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 2 (2024): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.79070

Abstract

Indonesia is rich in diversity of medicinal plants, vital in traditional medicine and the pharmaceutical industry. However, overharvesting, along with population growth, land use changes, deforestation, and climate change, endanger these plants. This review investigates the potential of endophytic endosymbiont as an alternative. These endosymbionts can synthesise bioactive compounds similar to those found in medicinal plants.  This study compiled data from various sources on endophytic fungi and their bioactivity. The review aims to categorise Indonesian medicinal plants, to identify their associated endophytic fungi from different plant parts, and to assess their bioactivity. The results revealed numerous medicinal plant families and a variety of endophytic fungi isolated from fruits, leaves, twigs, bark, roots, and rhizomes. These fungi exhibited bioactivities, including antioxidant, anticancer, antidiabetic, and antimicrobial effects, with metabolites such as alkaloids, flavonoids, peptides, phenols, polyketides, quinones, steroids, and terpenoids. Fusarium and Colletotrichum were the most common endophytic fungi found. Notably, the biological activity was consistent among endophytic fungi from various host organs, but variations were observed according to the host's geographical origin. This suggests that Indonesia's diverse geography influences metabolite production and activity. However, the same host plant may harbour different species in distinct organs. These findings indicate that endophytic fungi within medicinal plants represent a promising source of bioactive compounds for future Indonesian medicine production. Future research should explore metabolite compounds and bioactivity across different geographical regions.