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Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
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Articles 40 Documents
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Search results for , issue "Vol. 14 No. 1 (2024)" : 40 Documents clear
Characterization of Starch Granaule of Local Water Yam (Dioscorea alata L.) from Lermatang, Tanimbar Island District, Maluku: Characterization of Starch Granule of Dioscorea alata L. Sinay, Hermalina; Karuwal, Ritha Lusian; Fauziah, Fauziah; Anaktototy , Helena
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.18

Abstract

Most tuberous crops, for instance, the yam, whose scientific name is Dioscorea alata, have food reserves in the form of starch in tubers. Characterization of starch grains of a plant species is pertinent to uncover information on plant taxonomic traits and identify their potential use as food and industrial raw materials. The study aimed to determine the characteristics of starch grains of water yam from Lermatang village, Tanimbar Islands Regency. This study isolated and submitted six accessions of water yam in Lermatang Village. The morphological inspection of the plants used the Descriptor for Yam from IPGRI, and sample preparation for observing starch grains employed the fresh section method. The six accessions of D. alata found in Lermatang village, Tanimbar Islands, were Uwi Merah, Uwi Gula, Uwi Pingingsian, Uwi Babulu, Uwi Akiakab, and Uwi Petatas. These yam varieties show differences in their tubers' shape and colors. The study found that starch granules of D. alata in each accession varied in terms of granule shape and hilus type. The starch granules revealed dominant triangular, oval, and elliptical shapes and eccentric and concentric hilus types. The starch grain diameter in the six accessions of Uwi was categorically large (44.88 – 57.5 µm), with Uwi Petatas being the largest. Previous reports have shown that accessions having large starch granule types could be further developed into an array of foods, thus conveying the promising use of the Uwi Petatas for such a purpose.
Soil Properties and Macrofauna Community in a Converted Intensive Rice Field into an Organic Polyculture in Malang Regency, Indonesia: Soil Properties and Macrofauna Community in a Converted Intensive Rice Field Inayah, Durrotul; Mustafa, Irfan; Arisoesilaningsih, Endang
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.13

Abstract

Farmers in Malang cultivated rice intensively since 2019 due to water availability but reduce yields. It might also reduce soil productivity and increase pest attacks, so the conversion field to organic polyculture was needed using sorghum and legumes. The research aims were to evaluate soil fauna dynamics and soil properties in the three, six, and 12 months after converting (mac) into organic polyculture. Soil and micro-climate factors were recorded including air temperature (°C), day length (hours), rainfall (mm), water content (%), organic matter content (%), electrical conductivity (mS.m-1), pH, and soil bulk density (g.cm-3). Soil macrofauna was sampled using hand sorting (20 x 20 x 10 cm) with five plots at each field. Identified soil macrofauna was used to determine the density, frequency, Important Value Index (IVI), Shannon-Wiener Diversity Index (H'), Evenness Index (E), Simpson Dominance Index (D), Diversity t-test, and Indicator Species. The Canonical Correspondence Analysis (CCA) was used to analyze the interaction among abiotic factors and macrofauna using PAST 4.05. Results showed that the improvement of soil properties including soil organic matter and soil macrofauna was recorded at 6 mac compared to the intensive rice field, and continuously at 12 mac. The richness, diversity, and evenness of soil macrofauna taxa were higher in the converted field than in the intensive one due to organic polyculture. Moreover, we recorded a better proportion of detritivores and predators in the converted field after 12 months. Based on Indicator Species analysis, the dominant fire ants (Solenopsis sp.) in the intensive rice field might be considered as a potential indicator of unhealthy soil in the intensive rice fields. Whereas in the converted field the dominancy of these ants greatly decreased. We concluded that within 6 months conversion using the organic polyculture improved soil properties.
The Effect of VP3 Biofertilizer and Compost Application on Red Spinach (Amaranthus dubius) and Green Spinach (Amaranthus viridis) Yield and Quality: The Effect of VP3 Biofertilizer and Compost Application on Red Spinach (Amaranthus dubius) and Green Spinach (Amaranthus viridis) Yield and Quality Arfarita, Novi; Masyhuri, Masyhuri; Sugiarto , Sugiarto; Higuchi, Takaya
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.04

Abstract

VP3 biofertilizer is a biological agent that has been formulated in previous research. It was applied once with compost and has been tested on red spinach and green spinach in the greenhouse to see its effect on yield and quality (chlorophyll content, anthocyanins, and shelf life at room temperature). Red spinach and green spinach had shown a significantly different growth response to applying biological fertilizers. VP3 biofertilizer with compost had the highest yield effect but was not significantly different from the recommended Nitrogen Phosphate Potassium (NPK) and Effective Microorganism4(EM4) treatment with compost. The application of VP3 Biofertilizer with NPK fertilizer showed a darker color (higher chlorophyll and anthocyanin content) than the application of biological fertilizers, but the values ​​were not significantly different. The application of VP3 biofertilizer with compost affected the shelf life of both red spinach and green spinach. Spinach plants remained fresh at room temperature for three days of storage, while the other treatments lost freshness on the 3rd day after harvesting and even loosened the leaf on the 4th day after harvesting. Therefore, VP3 biofertilizer has potential to increase the yield and quality of spinach and other vegetable crops. The addition of the application period of VP3 biofertilizer needs to be carried out and observed to increase the yield and quality of plants further. Canonical Variate Analyses (CVA) can distinguish the grouping of treatments based on the selected parameter. Biplot assessment provides information on the strong relationship between the yield of green spinach and red spinach and a number of leaves, leaf area, and plant height. The application of VP3 biofertilizer with compost had a significant effect on the yield and quality and produced different effects between treatments, both in the case of green spinach and red spinach.
The Cytotoxic Activity of Marine Sponge-Derived Fungus Aspergillus nomius NC06 Against HT29 Colon Cancer Cells: Cytotoxic Activity of A. nomius NC06 Against HT29 Cancer Cells Artasasta, Muh. Ade; Djamaan, Akmal; Yanwirasti, Yanwirasti; Taher, Muhammad; Djamaludin, Heder; Siswanto, Siswanto; Handayani, Dian
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.05

Abstract

The study of natural products from marine-derived fungi has been interesting tense to researchers as drug discovery sources. Marine fungus from West Sumatera, Indonesia repeatedly showed their potential for cytotoxic and antimicrobial activities.  This study aims to determine the cytotoxic activity against HT29 colon cancer cells of each fraction of ethyl acetate extracts from Aspergillus nomius NC06 derived from marine sponge Neopetrosia chaliniformis. A. nomius was cultivated with rice as a growth medium and extracted with ethyl acetate solvent and evaporated in vacuo to obtain ethyl acetate extract. Furthermore, the compounds of ethyl acetate extract were separated with the VLC (Vacuum Liquide Chromatography) method. Five fractions were obtained, which further investigated their cytotoxic activity against HT29 colon cancer cells by using an MTT assay. The result showed that fractions I and III were categorized as potential fractions due to their IC50 value of 13.12 ± 0.39 μg/mL and 2.59 ± 0.19 μg/mL, respectively. It was also supported by ANOVA to measure the effect of each concentration (0.1; 1; 10; 100 μg/mL) of each fraction on the viability percentage of HT29 cells with p < 0.005.
An Overview of Natural Inert Dust Utilization Against Stored-Product Pests as Part of Integrated Pest Management: An Overview of Natural Inert Dust Utilization Against Stored-Product Pests Mario, M. Bayu; Patasik, William Yeremia; Tang, Muh. Ridha Taqwa; Muhammad, Mukhti; Rosyada, Amrina; Jamal, Ahmad Arisandi; Nurwahida; Kafle, Leknath; AM, Samir; Brugman, Eirene; Fernando, Ito
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.15

Abstract

Natural inert dust has been used as a grain protectant since the ancient Aztecs of Mexico to this modern era. Natural inert dust is divided into three groups: the first group includes sand, kaolin, paddy husk ash, wood ash, and clay; the second group includes mineral dust; and the third group includes natural silicas such as diatomaceous earth and zeolite. Natural inert dust has a unique mechanism for killing insect pests. Inert dust particles penetrate the insects’ exoskeleton, causing dehydration through the cuticle. Relative humidity is a crucial factor affecting the efficacy of inert dust application. Inert dust has been traditionally used by farmers, which impacts the insects, such as decreasing population, no insect resistance, and being environmentally friendly. Problems of using inert dust include visible residues on grain, airborne dust, reduced flowability, bulk density reduction, and adverse effects on downstream processing machinery. Moreover, inert dust is a very light product, thus it may cause human respiratory illness. The inert dust can be applied to the smaller or larger storage containers. Natural silica can be readily integrated into modern agriculture as a pest management solution.
Natural Product Testing: Selecting in vivo Anticancer Assay Model : In vivo anticancer assay model Saad, Dr. Norazalina; Chia, Suet Lin; Che Abdullah , Che Azurahanim; Sulaiman, Fadzil
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.16

Abstract

Phytochemicals and other natural products have been suggested to be effective adjuvants to conventional therapy to reduce potential side effects that arise from cancer treatments. Various natural compounds and synthetic analogues have been studied for their potential anti-cancer properties in the last decade. Prior to administering them to patients in a clinical setting, drug development necessitates a sequence of preclinical testing, beginning with in vitro and progressing to in vivo studies. While numerous drugs and plant extracts were reported to be active in vitro, only several of them had reproducible activity at the tested doses in vivo. Furthermore, breakthroughs in manipulating gene editing have aided scientists in accurately replicating human diseases in animal models.  Therefore, during drug development, in vivo studies are of tremendous help in evaluating a drug candidate's safety, toxicity, and efficacy in complex physiological and biochemical states. Hence, for this purpose, it is crucial to apply and select the animal model that is the most suitable to represent the studied disease or biological process. The current review summarizes various animal models that have been considered for in vivo testing, their advantages and disadvantages, and the experimental guidelines for conducting animal studies.
In Vitro Study of Garcinia dulcis (Roxb.) Kurz Leaves Extract against Hepatitis C Virus: Garcinia dulcis Leaves Extract against Hepatitis C Virus Apriyanto, Dadan Ramadhan; Hartati, Sri; Sri Wahyuni, Tutik; Inge Lusida, Maria
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.20

Abstract

Garcinia dulcis is a plant commonly used in traditional medicine in tropical regions. It has anti-bacterial, anti-inflammatory, anti-tumor, and anti-malaria properties. Despite its potential benefits, studies on its effectiveness as an antiviral are limited. This study investigated the antiviral properties of G. dulcis leaf extract against the JFH-1 strain genotype 2a as a treatment for Hepatitis C Virus (HCV). Huh7it-1 cells infected with the HCV genotype 2a strain JFH-1 were used to determine the antiviral effect of methanol extracts of G. dulcis leaves (GD-LE). GD-LE antiviral activity was investigated using a focus-forming assay for anti-HCV and 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay for cytotoxicity. A time-of-addition study of anti-HCV was also performed to determine the mode of action of GD-LE. The results showed that GD-LE inhibited HCV replication with an IC50 of 17.06 µg/mL without giving any toxicity to the cells. The mode-of-action of GD-LE was found to inhibit HCV replication in the co-addition and post-infection stages. These findings suggest that GD-LE could be a promising candidate for anti-HCV treatment. However, further study regarding GD-LE bioactive compound isolation needs to be done to prepare an effective antiviral.
Extract of Black Rice (Oryza sativa L. ‘Sembada Hitam’) Bran Protect Cytotoxicity of Hydrogen Peroxide on Vero Cells in a Short Time Incubation: Black rice bran ‘Sembada Hitam’ protect cytotoxicity of H2O2 Aprilianti, Suci Hari; Purwestri, Yekti Asih; Saragih, Hendry T.S.S.G; Nuriliani, Ardaning
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.01

Abstract

Oxidative stress induced by hydrogen peroxide (H2O2) can lead to cellular damage, contributing to degenerative diseases and aging. Black rice bran is a functional food known for its antioxidant properties, which are crucial in reducing the adverse effects of oxidative stress and maintaining redox balance. In this study, we aimed to investigate the protective effect of the extract of black rice bran (EBRB) 'Sembada Hitam' on Vero cells against H2O2 toxicity. To evaluate the protective effect, a co-culture method was employed, and cell viability was assessed using the MTT assay. Additionally, cell growth was examined through trypan blue staining. Vero cells were exposed to different concentrations of H2O2 and EBRB for a 24-hour period. The results demonstrated that EBRB at concentrations of 15.625, 250, and 500 μg/mL exhibited a protective effect on Vero cells exposed to H2O2 at concentrations of 100, 200, and 400 μM, respectively. Notably, when Vero cells were treated with EBRB at concentrations of 250 or 500 μg/mL for five days in conjunction with H2O2 exposure at concentrations of 200 or 400 μM for 24 hours, a significant decrease in cell viability was observed on day 3. Based on the collective findings, it can be concluded that EBRB has the potential to protect Vero cells against H2O2 -induced toxicity, but primarily during a short-term incubation period. Overall, this study highlights the protective properties of EBRB against H2O2 -induced cellular damage and emphasizes the importance of further investigations to fully elucidate the underlying mechanisms and potential long-term effects of EBRB on cell viability.
Chemical Compounds and Antibacterial Activity of Thymus Vulgaris Leaves’ Ethanolic Extract Against Salmonella typhimurium: Thymus vulgaris Against Salmonella typhimurium Abousouh, Emad Khaleefah; Endharti, Agustina Tri; Santoso, Sanarto; Santosaningsih, Dewi
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.09

Abstract

Invasive non-typhoidal Salmonella is an important pathogen that causes life-threatening bloodstream infections. Antibiotic resistance in non-typhoidal Salmonella has emerged as a result of the increasing use of antibiotics; therefore, an alternative source of therapeutic agents is required. This study aimed to investigate the active compound and antibacterial activity of Thymus vulgaris leaves’ ethanolic extract against Salmonella typhimurium. Thin-layer chromatography was performed to identify the active compound in the ethanolic extract of T. vulgaris leaves. The antibiotic effect of the extract was carried out by agar dilution assay using the following concentrations: 0%, 2.5%, 5.0%, 7.5%, 10.0%, 12.5%, and 15.0% (w/v). Thymol and carvacrol were detected in the thin-layer chromatography. The minimum inhibitory concentration of the extract was 10.0% (w/v). In conclusion, T. vulgaris leaves’ ethanolic extract demonstrated antimicrobial activity against S. typhimurium. Further investigation is required to analyze the role of thymol and carvacrol as active compounds against S. typhimurium.
The Correlation of Avifauna Diversity and Its Habitat: An Observation Study at Mount Kawi Slope Waterfall, East Java: Correlation of Avifauna Diversity and Its Habitat Lutfita Fitriana; Nia Kurniawan; Amin Setyo Leksono
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.11

Abstract

Avifauna inhabiting mountain forest ecosystems on Java Island face severe threats from anthropogenic disturbances. Despite this, the diversity of avifauna in specific areas, such as the Hutan Lindung Pegunungan Putri Tidur (HLPPT) on the slopes of Mount Kawi, remains poorly monitored. This study focuses on assessing avifauna diversity and communities in the tourist area of Mount Kawi slope waterfalls, situated on both the southeast side in Malang Regency and the north side in Batu City. The research, conducted from January to March 2022, examined eight waterfall locations (Baung, Glotak, Luksongo, Parangtejo, Manten, Tengah, Rondo, and Sumberpitu) using the roaming method with Visual Encounter Survey (VES) and Audiovisual Encounter Survey (AES) techniques. Data analysis included considerations of conservation status, local distribution, feed specialists, diversity indices (Taxa Richness, Shannon-Wiener Diversity Index (H’), Simpson Index (D), Evenness Index (E)), and Important Value Index (IVI) as an assessment of avifaunal diversity. Researchers employed Microsoft Excel and Principal Component Analysis (PCA) to determine preferences for each character diversity index and abiotic factors in each location. Across the site, 84 species from 33 families were identified. Notably, seven species were classified as at risk (4 near-threatened, two vulnerable, and one endangered), and nine avifauna were protected. The correlation of avifauna diversity to biotic and abiotic factors was positive. These results provide essential data for further conservation management, emphasizing the protection of the forest area, tourists, waterfalls, and slopes of Mount Kawi. Additionally, studying spatial and temporal distribution is crucial for future avifauna species re-inventory efforts.

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