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International Journal of Biosciences and Biotechnology

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Published by Universitas Udayana

ISSN : 23033371 EISSN : 26559994 DOI : -

Core Subject : Science, Social,

Biochemistry, Genetics & Molecular Biology Environmental Science

International Journal of Biosciences and Biotechnology provides a unique venue for publishing original researches in biosciences and biotechnology, and ensures that authors could reach the widest possible audience. It publishes both full-length articles and short communications on all aspects of biotechnology and biosciences

Arjuna Subject : -

Articles 160 Documents

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HEXOKINASES IN BREAST CANCER S. Kharitonov; A. Zikiriahodzhaev; M. Ermoshchenkova; A. Sukhot’ko; M. Fedorova; E. Pudova; B. Alekseev; A. Kaprin; A. Kudryavtseva
International Journal of Biosciences and Biotechnology Vol 4 No 2 (2017)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Hexokinases are one of the key enzymes involved in the process of glycolysis. The level of expression of hexokinases is widely studied in breast cancer as a possible marker of unfavorable prognosis and aggressiveness of tumors. The level of expression of hexokinase may reflect the level of glycolysis activation and, thus, indicate samples with the most altered cellular metabolism.

DETECTION OF CITRUS VEIN PHLOEM DEGENERATION (CVPD) DISEASE BY POLYMERASE CHAIN REACTION (PCR) AND PROTEIN ANALYSIS USING SDS PAGE (A Review) I Gede Putu Wirawan; Ketut Srie Marhaeni Julyasih
International Journal of Biosciences and Biotechnology Vol 3 No 1 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.933 KB)

Citrus Vein Phloem degeneration (CVPD) is the most important disease and a major cause of yield loss citrus plantations in almost all countries, especially Asia and Africa. CVPD disease is caused by gram negative bacteria, Liberobacter asiaticum L. Pathogens can not be cultured in vitro, but can be detected by PCR using 16S rDNA fragment as a primer and by electron microscopy. The use of PCRusing specific primer pair can detect infected plants more accurately. Citrus plant that infected by CVPD disease contain a very specific protein that produced by the pathogen. The specific protein molecules can be detected by comparing the protein bands of infected citrus plants and the healthy one which have molecular weight of approximately 16 kDa and 66 kDa, whereas in healthy citrus plant that protein molecule was not found.

Guide for Authors Guide for Authors
International Journal of Biosciences and Biotechnology Vol 4 No 1 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Guide for Authors

MERCURY ACCUMULATION IN GOLD MINE TAILING BY SWEET SORGHUM INOCULATED WITH CHROMIUM UPTAKE ENHANCING RHIZOBACTERIA Desi Utami; Sachiko Takahi; Irfan Dwidya Prijambada
International Journal of Biosciences and Biotechnology Vol 1 No 2 (2013)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.91 KB)

Small scale gold mining provides an important source of income for miners in rural communities whereeconomic alternatives are extremely limited. However, it releases mercury which damages the environmentand poses risks to those in the nearby community. Phytoremediation is considered as a simple and costeffective method for the cleanup of heavy metal from contaminated soil. Phytoremediation is a technologythat uses plants to degrade, extract, contain, or immobilize contaminants from soil and water. In particular,phytoextraction is the uptake of contaminants by plant roots and translocation within the plants to shootsor leaves. Contaminants are then removed by harvesting the plants. The objective of this research is toexamine the ability of sweet sorghum and its inoculation using chromium uptake enhancing rhizobacteriafor phytoremediation of gold mine tailing. Two varieties of sweet sorghum were used in this experiment, i.e.FS501, and KCS105. The seed of the sweet sorghum was sown in mercury containing tailing or a mixture ofit with uncontaminated soil. Plant biomass and its mercury content were determined 35 days after sowing.Only FS501 was able to grow on tailing containing 26.94ppm of mercury. Inoculation of the sweet sorghumwith the rhizobacteria does not aff ect its biomass but increase its mercury uptake signifi cantly. Mixing thetailing material with uncontaminated soil causes an increase in biomass but reduce the mercury content inplant signifi cantly. As a result, the amounts of removable mercury decrease signifi cantly. Inoculation withthe rhizobacteria has no eff ect on mixed material

GASTROINTESTINAL PARASITE INFECTION ON BALI CATTLE RAISED IN SEMI-INTENSIVE FARMING SYSTEM IN DOMPU, SUMBAWA ISLAND: A PRELIMINARY STUDY Made Sriasih; Oscar Yanuarianto; Dahlanuddin Dahlanuddin; William Ernest Pomroy
International Journal of Biosciences and Biotechnology Vol 6 No 1 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

To date, there are very few studies have been carried out on the effect of parasite infection on Bali cattle, and more importantly, there is no published data on the epidemiology of worm infections on Bali cattle in the island of Sumbawa, Indonesia. This research aimed to undertake a preliminary study on gastrointestinal (GI) parasitic infection on Bali cattle raised under semi-intensive farming system in five different sites in district of Dompu, Sumbawa island. Fecal samples of 76 Bali cattle, from three farmer groups namely site number 1, 4 and 5, were collected, processed and examined under a microscope, with 100x magnification, for egg fecal counting or oocyst fecal counting using the modified McMaster technique. The number of eggs/oocysts observed was then determined and identified. The results show that the eggs/oocysts ranged from 50-14800. The types of GI parasites identified were Strongyloides sp, Strongyle sp, Toxocara sp, Trichuris sp, and Coccidia. Coccidia oocyst is a type of parasite which was found with the highest percentage of 23.68% followed by Strongyle sp. (18.42%) and Toxocara sp. (10.52%). The overall prevalence of GI parasitic infection in cattle accounted for 43.42%. Young calves (0-3 months old) was more susceptible to GI parasitic infection among other age groups. This preliminary data could be used as a basic data on gastrointestinal parasitic infection in Dompu district. These results may also indicate the need to develop control strategies aiming at lowering the gastrointestinal parasitism.

Agrobacterium tumefaciens-MEDIATED IN PLANTA TRANSFORMATION METHOD FOR THE SoSPS1 GENE IN CITRUS PLANTS (Citrus nobilis L.) Ni Putu Ayu Erninda Oktaviani Suputri; Rindang Dwiyani; Ida Ayu Putri Darmawanti; Bambang Sugiharto
International Journal of Biosciences and Biotechnology Vol 7 No 1 (2019)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

The SoSPS1 gene of sugar cane plants previously subjected to Agrobacterium tumefacienmediated cloning was to be transferred to citrus plants to increase metabolism of sucrose in plant. The T-DNA harbored the SoSPS1 gene under the control of the CaMV 35S promoter from the cauliflower mosaic virus and contained the NPTII gene (kanamycin resistance gene) as a selectable marker for transformant selection. Generally, gene transformation in plants is carried out by tissue culture. However, tissue culture has several disadvantages such as its being time-consuming, its sometimes resulting in somatic mutations and somaclonal variations, and the requirement of sterile conditions in the procedure of gene transfer. In planta transformation is a useful system for those plants that lack tissue culture and regeneration system. The main function of in planta transformation is to recover the advantages of tissue culture as an efficient, quick method, including its ability to produce a large number of transgenic plants and to accumulate a high concentration of total soluble protein in short time. There are two procedures of in planta transformation for the seeds of citrus plants, namely “prick and coat” and “seed tip-cutting and imbibition”. In the prick and coat method, seeds are pricked on their entire surfaces and smeared with a suspension of Agrobacterium tumefaciens. In the seed tip-cutting and imbibition method, on the other hand, seeds are cut at the tip and soaked in a suspension of Agrobacterium tumefaciens. The leaves derived from seeds treatment were taken as samples for DNA extraction and PCR using primers of the NPTII gene (Forward: 5’-GTCATCTCACCTTCCTCCTGCC-3’; Reverse: 5’-GTCGCTTGGTCGGTCATTTCG-3’). This research found that only the seed tip-cutting and imbibition plants amplified along the 550-bp band, while those of the prick and coat method did not. Additionally, the T-DNA was successfully integrated into the genome of the plants treated with the seed tip-cutting and imbibition method but not with the prick and coat.

DNA POLYMORPHISM AND GENETIC DIVERSITY OF MANGO (Mangifera sp.) GERMPLASM IN TROPICAL ISLAND Putu Suwardike; I Nyoman Rai; Rindang Dwiyani; Eniek Kriswiyanti
International Journal of Biosciences and Biotechnology Vol 7 No 1 (2019)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Creation of new superior varieties of mango through the empowerment of local genetic resources requires information about the potential properties of mango germplasm, including DNA polymorphism and its genetic diversity. This research aimed to obtain basic data on DNA polymorphism, genetic relationship, genetic similarity level, and molecular accession of Bali’s unique local mango. Sampling was conducted in four regencies in the Province of Bali. DNA preparation, PCR, and microsatellite analysis were carried out at the Laboratory of Genetics and Plant Breeding, Faculty of Agriculture, Gadjah Mada University. It found 44accessions of mangoes in Bali. The test results with 10 pairs of SSR markers showed that all primers produced polymorphic loci. There were 825 amplified DNA bands. Primer of AY31 produced the highest number of loci, which were 14 loci, while AY21 produced the fewest loci, i.e., 3 loci. Forty-four mango accessions showed a genetic similarity coefficient of 0.27 to 0.97. At a coefficient of 0.27, accessions were divided into 2 major groups: group A and group B. Group A consisted of two accessions, namely, KRA-005 and BDG-006 (Mangifera foetida Lour.), which had a similar coefficient of 0.657. Group B was divided into twosmaller groups, namely, groups B1 and B2, at a similarity coefficient of 0.342. Group B1 consisted of 39 accessions, while group B2 consisted of 3 accessions. Accessions Madu Anggur, Gading, Sambuk Mengwi, Kakul and Pakel Sulangai were identified as having unique alleles.

INDUCED IN VIVO MUTAGENESIS USING COLCHICINE IN HYDROGEN PEROXIDE ON M2 COWPEA, YARDLONG BEAN AND COMMON BEAN I Gede Ketut Susrama; Ni Luh Made Pradnyawathi
International Journal of Biosciences and Biotechnology Vol 7 No 1 (2019)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Research on doubling plant chromosome using colchicine has been carried out for years with water as its solvent. In this study, an innovation was carried out by trying to do colchicine treatment in hydrogen peroxide. In this in vivo induced mutagenesis research, colchicine in hydrogen peroxide was administered as mutagen on seeds three species of crops i.e. mutan M2 cowpea, yardlong bean, and common bean by soaking method. The results of this research showed that three times multistep mutant (M3) of cowpea was having mutant characteristics that the shape of its leaflet changed from rhombus to ovate. Besides, both yardlong bean varieties used in this research (BLR and KTR) showed indication of a mutant crop that having compound leaves with more than three leaflets in a stalk. Common bean of PTW variety was not attacked by any aphid but it was attacked by leafminer. Finally, we found “rainbow common bean” from this research.

ANALYSIS OF LIMONENE AND OTHER ANTIOXIDANTS IN COMMERCIALESSENTIAL OIL PRODUCTS COMPARED TO HOMEMADE VIRGIN COCONUT OIL Ni Made Suaniti; I Wayan Bandem Adnyana; Manuntun Manurung; Oka Ratnayani; Raisyah Anjani
International Journal of Biosciences and Biotechnology Vol 7 No 1 (2019)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Essential oil production in the market is very diverse with various brands labeled as antioxidants that have gained more attention in the society recently. The purpose of this study was to analyze limonene and other antioxidants content of the essential oils in the market compared to homemade virgin coconut oil. The method used was Gas Chromatography-Mass Spectrometry (GC-MS) and Fourier-transform infrared (FTIR) Spectroscopy. The infrared spectrogram showed the presence of alkanes and esters respectively at wave numbers of 3000-2850 and 1750-1730 cm-1. Chromatogram results showed the presence of limonenecompounds and some antioxidants with a fairly good separation in the several types of oils, which was not detected in the homemade virgin coconut oil.

THE EFFECTIVENESS OF INDIGENOUS ENDOMYCORRHIZA AND RHIZOBIUM INOCULUM IN INCREASING NUTRIENT UPTAKE AND YIELD OF SOYBEAN IN DRY LAND Yosni Kiuk; I Nyoman Rai; Anak Agung Istri Kesumadewi
International Journal of Biosciences and Biotechnology Vol 7 No 1 (2019)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Increasing the productivity of soybeans in dry land can be done by designing a package of fertilizer technology based on biological materials using indigenous endomycorrhiza and Rhizobium. The application of indigenous endomycorrhizal biofertilizer combined with Rhizobium is expected to be able to improve nitrogen and phosporus uptake so as to increase the productivity of soybeans in dry land. The experiment used two-factor factorial randomized block design. The first factor was dose of endomycorrhizal spores of Glomus that consisted of four levels, namely, treatment without endomycorrhiza inoculation, dose of 50endomycorrhizal spores, dose of 100 endomycorrhizal spores, and dose of 150 endomycorrhizal spores. The second factor was inoculation of Rhizobium inoculum that consisted of two levels, namely, treatment without Rhizobium inoculation and Rhizobium inoculation. The results showed that combination of 50 endomycorrhizal spores with Rhizobium inoculation gave the best effect on the number of effective root nodules per plant, endomycorrhizal infection per plant, nutrient uptake by plant, number of pods per plant, number of seeds per plant, and oven-dry weight of seeds per plant.

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