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INDONESIA
METAMORFOSA Journal of Biological Sciences
Published by Universitas Udayana
ISSN : 23025697     EISSN : 26558122     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
METAMORFOSA is an electronic scientific journal published periodically by the Master of Biology Udayana University, which includes scientific works in the field of Biology.
Arjuna Subject : -
Articles 316 Documents
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PEMANFAATAN TUMBUHAN PEKARANGAN SEBAGAI BAHAN OBAT ALTERNATIF DI DESA JIMBARAN, KECAMATAN KUTA SELATAN, KABUPATEN BADUNG, BALI Irawati Irawati; Eniek Kriswiyanti; A. A. Ketut Darmadi
Metamorfosa: Journal of Biological Sciences Vol 5 No 1 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i01.p10

Abstract

Penelitian ini bertujuan untuk mengetahui jenis-jenis tumbuhan pekarangan yang dapat dimanfaatkan sebagai obat alternatif, bagian tumbuhan yang digunakan, cara pengolahan dan penggunaan serta untuk mengobati penyakit apa saja yang. Penelitian dilaksanakan pada Oktober 2016 sampai Januari 2017 di 13 banjar di Desa Jimbaran dan identifikasi tumbuhan di Laboratorium Sruktur Perkembangan Tumbuhan Program Studi Biologi FMIPA Universitas Udayana. Prosedur penelitian meliputi observasi lapangan, wawancara dengan metode purposive sampling dan snow ball dalam pemilihan responden, dokumentasi dan identifikasi. Data dianalisis secara deskriptif kualitatif. Dari hasil penelitian diperoleh 65 jenis (36 suku) dan suku yang paling banyak dimanfaatkan adalah Zingiberaceae. Bagian tumbuhan yang paling banyak digunakan adalah daun (47,1%), kemudian diikuti rimpang (12,9%), buah (11,4%), akar (7,1%), kulit batang (7,1%), bunga (4,3%), getah (2,9%), umbi (2,9%), biji (2,9%)dan akar gantung (1,4%). Cara pengolahan tumbuhan obat yang paling banyak adalah dengan cara direbus (44,8%), lalu diminum. Penyakit yang dapat diobati yaitu sebanyak 47 penyakit dalam dan 21 penyakit luar.
RAGAM ALEL DNA MITOKONDRIA MASYARAKAT SOROH PANDE DI BALI DENGAN METODE PCR-RFLP Ni Putu Senshi Septiasari; I Ketut Junitha; Ni Nyoman Wirasiti
Metamorfosa: Journal of Biological Sciences Vol 4 No 2 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i02.p12

Abstract

Masyarakat Bali mengelompokkan diri berdasarkan soroh atau garis keturunan tetapi bukti genetik pengelompokkan tersebut belum diketahui. Salah satu soroh/ klan yang memiliki persebaran yang luas di seluruh Bali adalah soroh/ klan Pande. Penelitian ini dilakukan untuk mengetahui ragam alel DNA mitokondria masyarakat soroh Pande di Bali dengan metode Polymerase Chain Reaction-Fragment Length Polymorphism (PCR-RFLP) yang berguna untuk kepentingan forensik. Sampel sel epitel mukosa mulut diambil dengan cara purposive sampling yang terdiri dari 35 sampel warga Pande. Tahapan penelitian meliputi ekstraksi DNA dengan metode fenol-kloroform yang telah dimodifikasi, amplifikasi DNA pada daerah D-loop DNA mitokondria (mtDNA), digesti produk PCR dengan lima macam enzim restriksi dan elektroforesis dengan menggunakan gel agarose 1 % dan PAGE 10%. Penelitian ini mendapatkan sembilan ragam alel. Digesti dengan enzim Hae III, Hinf I, Mbo I dan Dde I mendapatkan masing-masing dua tipe pemotongan sedangkan enzim Hind III tidak memiliki situs pengenalan pada daerah D-loop DNA mitokondria. Enzim Hind III tidak dapat digunakan untuk membedakan profil DNA antar individu karena bersifat monomorfik.
Metode Deteksi Carbapenem Resistant Enterobacteriaceae Ni Wayan Eka Putri Gayatri Kastawa; Yan Ramona; N.N. Dwi Fatmawati.
Metamorfosa: Journal of Biological Sciences Vol 7 No 1 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i01.p07

Abstract

Carbapenem Resistant Enterobacteriaceae (CRE) is a group of Enterobacteriaceae resistant to Carbapenem antibiotics (Imipenem, Ertapenem, Meropenem, Doripenem). One species of Enterobacteriaceae frequently showing resistant characteristic to Carbapenem is Klebsiella pneumoniae. Early detection methods on the CRE existence (phenotypically or genetically) are urgently needed. The methods should be able to detect carbapenemases or genes encoding these types of enzymes. In the recent years, phenotypic characteristics can be done by applying methods, such as Modified Hodge Test (MHT), Carba Nordmann-Poirel (Carba NP), or Modified Carbapenem Inactivation Method (MCIM). To detect the presence of genes encoding production of carbapenemases, Polymerase Chain Reaction (PCR) is the most reliable method available in the recent years. Keywords: Carbapenem Resistant Enterobacteriaceae (CRE), Phenotype, Genothype, Modified Carbapenem Inactivation Method (MCIM), Modified Hodge Test (MHT), Carba Nordmann-Poirel, Polymerase Chain Reaction (PCR).
Induksi Akar dan Pertumbuhan Stek Pucuk Jirak (Eurya acuminata DC.) dengan Pemberian Beberapa Zat Pengatur Tumbuh Pada Berbagai Media Tanam Tressa Pratywi Gupitasari; Zozy Aneloi Noli; Suwirmen Suwirmen
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p19

Abstract

The research about roots induction and growth of shoot cutting of Jirak (Eurya acuminata DC.) by adding some growth regulator solutions in various planting medium, was conducted from May until August 2017 in Greenhouse and Plant Physiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Science, Andalas University. The aims of the research to find out effect of providing growth regulator solution with different types, used of planting medium and interaction of both factors the best to roots induction and growth Jirak (E. acuminata DC.) by cutting. The research used Complete Randomized Design (CRD) factorial with two factors and three replications. Factors A were growth regulator solution (a0: control, a1: IBA 100 ppm, a2: NAA 100 ppm, a3: IAA 100 ppm) and factors B were various planting medium (b0: garden soil, b1: sand, b2: charcoal husk). The research result were adding some growth regulator solutions and various planting medium significanly affected roots induction and growth of jirak and there is interaction on the high of plant. The treatment of 100 ppm IAA and treatment using sand medium obtain the highest result in increasing roots induction and growth of shoot cutting of Jirak.
PEMANFAATAN SAMPAH ORGANIK KOTA SEBAGAI BAHAN DASAR PUPUK ORGANIK CAIR (POC) UNTUK PERTUMBUHAN Lactuca sativa L.var. crispa DENGAN SISTEM VERTIKULTUR Eka Muliani; Zozy Aneloi Noli; Periadnadi Periadnadi
Metamorfosa: Journal of Biological Sciences Vol 4 No 2 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i02.p03

Abstract

Pemanfaatan kembali sampah organik sebagai bahan dasar pupuk organik cair (POC) merupakan salah satu cara dalam mengatasi permasalahan sampah terutama di perkotaan. POC yang dihasilkan dapat digunakan untuk meningkatkan pertumbuhan tanaman, seperti Lactuca sativa var. crispa (selada merah). Penelitian ini dilakukan untuk mengetahui pengaruh konsentrasi POC berbahan dasar sampah organik kota terhadap pertumbuhan selada merah yang ditanam dengan sistem vertikultur. Penelitian dilakukan dengan mengunakan Rancangan Acak Kelompok (RAK) yang terdiri dari 5 perlakuan dan 5 kali ulangan. Perlakuan adalah konsentrasi POC (0%, 10%, 20%, 30% dan 40%). Hasil penelitian menunjukkan bahwa konsentrasi POC memberikan pengaruh yang berbeda nyata terhadap tinggi tanaman, jumlah daun baru, luas daun, berat basah dan kadar klorofil total selada merah tetapi tidak berpengaruh nyata terhadap berat kering tanaman. Konsentrasi POC terbaik untuk pertumbuhan selada merah adalah 10%.
Produksi Virgin Coconut Oil (VCO) yang Dikatalis oleh Lactobacillus plantarum dan Enzim yang Terkandung dalam Sari Nanas Helda Thorikul Affan; Yan Ramona; Ni Luh Suriani
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p03

Abstract

Indonesia is a tropical country with an area of coconut plantation of 3.7 Ha. The fruits of this plant have been used as materials in the production of Virgin Coconut Oil (VCO). Such oil is known to contain higher Medium Chain Fatty Acid (MCFA) or Medium Chain Triglycerides (MCTs) than original frying oil. The MCTs of this oil is very useful to avoid obesity, to reduce total cholesterol as well as to function as anti-microbes. These have been considered as a cheap solution to solve health related problems. The main objective of this research was to produce VCO catalyzed by pure culture of Lactobacillus plantarum isolated from a commercial VCO (Vici’O) combined with enzymes extracted from pineapple fruits. The study was conducted at the laboratory of Microbiology, Faculty of Mathematics and Sciences, Udayana University. The study was started with isolation and identification of Lactobacillus sp. from a commercial VCO. The VCO production method combined the two starters of Lactobacillus isolates (at concentrations of 0 to 10% v/v) and pineapple juice with various volumes (0 to 60 mL). In the first stage of the project, 3 potential Lactobacilli isolates (Sp1, Sp2, and Sp3) were successfully isolated and were confirmed to be Lactobacillus sp. In the main experiment, combination of 10% v/v Lactobacillus starter and 60 mL pineapple juice produced the highest VCO yield (43,17% v/v) with improved organoleptic properties, lower water content, lower saphonification, and lower peroxide than that specified by SNI No 7381: 2008.
KEANEKARAGAMAN JENIS TUMBUHAN DAN PENDUGAAN CADANGAN KARBON DI ATAS PERMUKAAN TANAH DI KAWASAN HUTAN BUKIT BARISAN BAGIAN BARAT KOTA PADANG . Yastori; . Chairul; . Syamsuardi; . Mansyurdin; Tesri Maideliza
Metamorfosa: Journal of Biological Sciences Vol 3 No 2 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i02.p02

Abstract

Indonesia has a vast forest area. The extent of Indonesia's forests is one of the natural resources are prone to damage due to human interests in meeting their needs. One of the damage that often occurs when current is forest fires. Forest destruction accounts for 20-25% of global CO2 emissions that contribute to climate change or global warming. Unspoiled forest with a diversity of plant species are long-lived and litter is a place to store a lot of carbon stocks (C) the highest. The aim of this study was to determine the diversity of plants and the amount of carbon stock above ground level in the forests of the Bukit Barisan of Padang, West Sumatra. Tree biomass was calculated on a plot of 20x20 m, 10x10 m pole, stake 5x5 m, for counting down plant biomass and litter on the plot with a size of 2x2 m (National Standardization Agency, 2011). Biomass calculated by the Ketterings et al. formula (2001). In Bukit Barisan Forest Area, West Sumatra, derived carbon content was 16.029,70 ton/ha. Diversity type was highest at tree level on Station 1, classified as very high diversty with diversity index (H’) 3.10.
Kadar Hormon Estrogen Dan Ketebalan Endometrium Tikus (Rattus norvegicus) Betina Ovariektomi Setelah Pemberian Ekstrak Daun Lamtoro (Leucaena leucocephala Lam. De Wit) Maria Antonia Margaretha Fernandez; Ngurah Intan Wiratmini; Ni Made Rai Suarni
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p15

Abstract

Lamtoro (Leucaena leucocephala Lam. De Wit) merupakan tanaman yang digunakan sebagai obat dan pakan ternak. Lamtoro mengandung zat aktif dari golongan senyawa streroid, alkaloid, flavonoid, polifenol dan tannin. Zat aktif yang terkandung pada lamtoro bersifat fitoestrogen karena mengandung golongan senyawa steroid, alkaloid dan flavonoid. Tujuan penelitian ini untuk mengetahui pengaruh pemberian ekstrak daun lamtoro terhadap kadar hormon estrogen dan ketebalan endometrium tikus (Rattus norvegicus) betina yang telah diovariektomi. Penelitian ini menggunakan Rancangan Acak Lengkap dengan empat perlakuan yaitu K- (kontrol negatif diberi minyak jagung), K+ (kontrol positif diberi estrogen sintetik 2 mg), P1dan P2 diberi diberi ekstrak daun lamtoro sebanyak 200 mg/kg BB dan 250 mg/kg BB). Masing masing perlakuan terdiri dari 6 ulangan sehingga digunakan 24 ekor tikus betina. Ekstrak daun lamtoro diberikan secara oral sebanyak 2 ml/ekor/hari selama 15 hari. Data yang diperoleh dianalisis dengan One Way ANOVA dan dilanjutkan dengan uji Duncan Multiple Range Test (DMRT). Hasil penelitian menunjukkan pemberian ekstrak daun Lamtoro (Leucaena leucocephala Lam. De Wit) dosis 250 mg/kg BB dapat meningkatkan kadar hormon estrogen serum darah dan ketebalan endometrium tikus betina yang diovariektomi. Kata kunci: Leucaena, Ovariektomi, Estrogen, Endometrium, Tikus
Toksisitas dan Repelensi Minyak Sereh Dapur [Cymbopogon flexuosus (Nees ex Steud.) W. Watson] terhadap Kecoak Jerman (Blattella germanica L.) Syafri Yana; Resti Rahayu; Mairawita Mairawita
Metamorfosa: Journal of Biological Sciences Vol 5 No 2 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i02.p13

Abstract

The purposes of this study are to find out the toxicity and repellency of lemongrass oil (Cymbopogon flexuosus) to the German cockroach (Blatella germanica). Study was conducted from August to October 2016 in Research Laboratories of Animal Physiology, Department of Biology, Faculty of Mathematics and Natural Sciences Andalas University Padang. This study used the glass jar method (contact) with three strains of German cockroaches (VCRU-WHO, KRS-BDG and HHB-JKT) and lemongrass oil. The average of lethal time after 6 hour exposure ware counted with a probit analysis until found value LC90. The results showed that based on value LC90 strain VCRU-WHO 15,20%, strain KRS-BDG 22,42% and strain HHB-JKT 19,28% after 6 hour exposure.
OPTIMASI DIGESTI ENZIM RESTRIKSI SacII PADA ISOLAT Mycobacterium tuberculosis H37Rv UNTUK DETEKSI MUTASI PROMOTER inhA PADA KASUS MDR-TB DENGAN METODE PCR-RFLP Ida Ayu Ratih Dwi Nugraha Putri; Sagung Chandra Yowani; I Nengah Wirajana
Metamorfosa: Journal of Biological Sciences Vol 4 No 1 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i01.p14

Abstract

The aim of this study was to optimize the digestion process of SacII restriction enzyme in Mycobacterium tuberculosis H37Rv isolate. SacII enzyme that have CCGC^GG restriction site could be used to detect the mutation at -24 promoter region of inhA M. tuberculosis. The mutation in this position is one of the causes of isoniazid resistance. The methods of this study were conducted in several step, respectively: DNA isolation of M. tuberculosis H37Rv, amplification of inhA promoter region of M. tuberculosis H37Rv using PCR method, and digest optimization using SacII. In this study, optimization of SacII digestion was conducted for formulaion and incubation time. The formulation was optimized by GM-ONE Buffer and GM-Buffer IV, meanwhile the incubation time was optimized in 1 hour, 2 hour, and 3 hour. The result of this study showed that SacII work optimally in GM-Buffer IV compared to GM-ONE Buffer and incubate for 3 hours. Under the optimal digest condition, SacII enzyme could be used to detect the mutation at -24 promoter region of inhA in the case of MDR-TB using PCR-RFLP method.

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