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ARI HAYATI
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Jurnal Ilmiah Biosaintropis (Bioscience-Tropic)
Published by Universitas Islam Malang
ISSN : 23382805     EISSN : 24609455     DOI : https://doi.org/10.33474/e-jbst.v7i2.305
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
Jurnal ini mengkaji fenomena dan temuan penelitian di bidang biologi dan ilmu-ilmu dasar (sains) lainnya serta bidang studi di wilayah tropis. Jurnal ini ditujukan untuk menemukan solusi alternatif dalam perkembangan ilmu biologi demi kesejahteraan masyarakat Indonesia dan Dunia.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Bioteknologi
Articles 270 Documents
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Analisis Konsentrasi Pestisida Klorpirifos pada Letal Ikan Mas (Cyprinus carpio) Rohimah, Ririn Alfiatu; Santoso, Hari; Syauqi, Ahmad
Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Vol 9 No 2 (2024): January 2024
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/e-jbst.v9i2.440

Abstract

One type of orghanophosphate pesticide used by farmers is chlorpyrifos. Uncontrolled use of pesticides has an impact on environmental pollution, especially aquatic biota. The purpose of this study was to analyze the acute concentration of chlorpyrifos pesticide against goldfish (Cyprinus carpio). The research method was experimental with preliminary research, acute toxicity test (LC50), real test (sub lethal). The concentration of treatment in experimental animals includes the sublethal test K0 (control) 0 ppm, K1 0.25 ppm, K2 0.30 ppm, K3 0.35 ppm, K4 0.40 ppm, K5 0.45 ppm. The preliminary test results obtained an upper lethal threshold value of 0.5 ppm and a lower lethal threshold value of 0.25 ppm. Acute toxicity tests ranged from K1 0.25 ppm to K5 0.45 ppm. The results of the acute toxicity test (LC50) at K1 0.25 ppm and K2 0.30 ppm showed experimental goldfish exposed to chlorpyrifos experienced abnormal behavior, namely increased operculum movement (opening and closing) with an average of K1 = 72.405; K2 = 43.130 causes goldfish to die quickly, swim on the surface, tend to approach the aerator, and move quickly without direction. The conclusion is that there is a significant relationship between the amount of pesticide concentration given to the survival of goldfish. Keywords:Goldfish (Cyprinus carpio), Chlorpyrifos pesticide
Pengaruh Variasi Konsentrasi Indole Butyruc Acid (IBA) Terhadap Pertumbuhan Anggrek (Dendrobium canaliculatum) Pada Media Tumbuh Moss Putih Fitri, Hanin Rahma; Rahayu, Tintrim; Jayanti, Gatra Ervi; Agisimanto, Dita
Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Vol 9 No 2 (2024): January 2024
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/e-jbst.v9i2.507

Abstract

Orchids are ornamental plants that are in demand by the public because of the beauty of their flowers and are very profitable prospects. However, orchids have a long growth phase, with one of the critical phases being acclimatization. The development of the acclimatization phase is a prerequisite for obtaining a high plantlet survival rate. Good plantlet survival is obtained with a good root system using a growth regulator auxin Indole Butyric Acid with white moss growing media. This study aimed to study the effect of variations in IBA concentration on faster root growth of Dendrobium canaliculatum orchids using white moss media. Plantlet Dendrodium canaliculatum seed culture from sub culture 3 and selected first, planted in white moss media, and treated twice a week for a month using 0.25, 0.50, 0.75, and 1.0 ml/L IBA and fertilizer NPK Mamigro 21-21-21 as much as 2 g/L. IBA concentration at 0.25 ml/L was the best concentration for the induction of new roots of Dendrobium canaliculatum orchids grown on white moss and had a high survival rate of 92%.
Respon Pertumbuhan Anggrek Dendrobium sp. terhadap Pemberian Ekoenzim dan Nanobubbles (NBs) CO2 Istifadah, Hayati; Rahayu, Tintrim; Jayanti, Gatra Ervi
Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Vol 10 No 1 (2024): Agustus 2024
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/ejbst.v10i1.547

Abstract

Genus Dendrobium merupakan salah satu anggrek yang banyak diminati karena mampu beradaptasi dengan berbagai kondisi lingkungan tumbuh. Faktor penting yang mempengaruhi pertumbuhan anggrek adalah pemupukan. Pemupukan dengan pupuk organik ekoenzim dan Nanobubbles (NBs) merupakan inovasi untuk meningkatkan pertumbuhan anggrek. Ekoenzim merupakan hasil fermentasi sampah dapur. Sedangkan NBs merupakan salah satu contoh teknologi nano yang memiliki diameter 1-100 nm. NBs telah banyak dimanfaatkan dalam berbagai bidang, berpeluang untuk pertumbuhan anggrek Dendrobium sp. Penelitian ini menggunakan metode eksperimental rancanan acak kelompok (RAK) dengan 10 perlakuan yaitu 0 mL/L (kontrol), 1 mL/L Ekoenzim, 2 mL/L Ekoenzim, 3 mL/L Ekoenzim, 4 mL/L Ekoenzim, 5 ml NBsCO2 dan 4 kombinasi NBsCO2 dan Ekoenzim. Parameter pengamatan meliputi jumlah daun, panjang daun, jumlah akar, panjang akar, tinggi tanaman, berat basah dan berat kering tanaman. Hasil penelitian ini menunjukkanpemberian Ekoenzim 1 mL/L memberikan respon terhadap pertumbuhan jumlah daun (9,67), panjang daun (8,30 cm), tinggi tanaman (9,63 cm), berat kering (0,40 g) dan berat basah (1,05 g) tanaman. Pemberian Ekoenzim 2 mL/L memberikan respon terhadap panjang akar (6,97 cm), sedangkan pemberian 1 mL/L Ekoenzim + 5 ml NBsCO2 memberikan respon terhadap pertumbuhan jumlah akar (14,67). Kata kunci: Pertumbuhan, Dendrobium, Ekoenzim, NBsCO2
Uji Nanoemulsi Ecoenzym Terhadap Bakteri Staphylococcus aureus anindita, Reza; Nathalia, Dede Dwi; Beandrade, Maya Uzia; Putri, Intan Kurnia; Sari, Afrinia Eka; Setyodewi, Elisabeth
Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Vol 9 No 2 (2024): January 2024
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/e-jbst.v9i2.553

Abstract

The discovery of eco enzyme as a fermented liquid from organic vegetable and fruit waste has been used as an organic plant fertilizer, disinfectant, hand sanitizer, antiseptic soap, and domestic waste degradation agent. However, trials of eco enzymes as raw materials for health preparations have not yet been carried out. Seeing this, trial research is needed on the use of ecoenzyme nanoemulsion against Staphylococcus aureus bacteria. This research aims to determine the antibacterial ability of ecoenzyme nanoemulsion against S. aureus bacteria. The design of this research is experimental. The samples used were ecoenzyme nanoemulsions with Formula 1 (F1) 20%, Formula 2 (F2) 25%, and Formula 3 (F3) 30%. This research includes making ecoenzyme, making ecoenzyme nanoemulsion, testing ecoenzyme nanoparticles, and testing ecoenezyme nanoemulsion against S. aureus bacteria using the Kirby-Baeur method. The results showed that the sizes of ecoenzyme nanoparticles in F1 20%, F2 25%, and F3 30% were 38.9 nm, 65.07 nm, and 146.2 nm, respectively. The results of the antibacterial ability test of nanoemulsion eco enzyme F1 20%, F2 25%, and F3 30% against S. aureus produced an inhibitory zone 4.3 mm (resistant), 4.3 mm (resistant), and 6.7 mm (resistant). This research concludes that the eco enzyme nanoemulsion is not effective in inhibiting the growth of S. aureus bacteria.
Efek Ekstrak Biji Kapas (Gossypium hirsutum) terhadap Kualitas dan Perkembangan Embrio Mencit (Mus musculus) Zayani, Nofri; Yulita, Ramadani
Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Vol 9 No 2 (2024): January 2024
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/e-jbst.v9i2.554

Abstract

The cottonseed contained gossypol which can reduce fertility by distrupting follicles and oocytes formation process and development. Folliculogenesis disorder can reduce quality and competence of oocytes to be fertilized. It has an impact on the quality and development of the embryo. The objective of this study was to evaluate the cottonseed extract effect on the quality and development of mice embryos. The research used a total experiment with completely randomized design consist of 4 treatments (0; 1.5; 2.1; and 2.7 g/kg BW EBK) and 6 replications. EBK are extracted maceration using ethanol 80%. EBK administration was orally injection for 24 days. Last day of EBK administration, mices were mated. Embryos were collected on the 4 days of pregnancy by flushing the uterine cornua, then development stage and quality of embryos were determined. Embryos were then cultured in vitro for 48 hours in culture medium PBS supplemented by 10% FBS. The result showed that EBK 2.7 g/kg BW dose can reduce embryos quality by finding retarded, degenerated, and unfertilized oocytes. The decreased embryos number and ability to progress to the blastocysts, expanded blastocysts, and hatched blastocysts stage occurred after culture for 48 hours. Retarded (4-8 cells) and degenerated embryos are not development in 24 hours culture. In conclusion, EBK is able reduce quality and development inhibit of mice embryos, so that it can be used as candidates for herbal contraception.
Uji Kepekaan Antibiotik Klebsiella pneumoniae dari Sampel Sputum, Darah, dan Pus Inggraini, Maulin; Ilsan, Noor Andryan; Nurfajriah, Siti
Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Vol 9 No 2 (2024): January 2024
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/e-jbst.v9i2.555

Abstract

Identifikasi Mikroplastik di Sungai Ngrowo, Tulungagung Kartikasari, Desi; Zunisnaini, Zunisnaini; Nurdianyoto, Indra
Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Vol 9 No 2 (2024): January 2024
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/e-jbst.v9i2.556

Abstract

Microplastics are synthetic organic polymers with size range 0.001-5 mm. The existance of microplastics disperse in the environment are harmful to human and natural ecosystems along with possess damage effects on aquatic organisms. The aim of the research was to determine types and abundance of microplastics in Ngrowo River Tulungagung. Sampling of microplastics was conducted at three locations, namely north city, center city and south city. The stages of the research were start from abiotic factors measurement of water continued with sampling, filtering and drying sample, purifying and separation of microplastics particle and observation using stereo microscope. The microplastics types found were fragment, fiber, filament, granule and foam with the highest average of abundance 13,450 particles.(m3)-1 at the center city. The colors of microplastics obtained were dark blue, transparent, chocolate, white, black, grey and red with the highest number was dark blue. Microplastics are known derived from run off road the plastic particle, plastic degradation of domestic waste, industry and others anthropogenic activities.
Limbah Fermentasi Kombucha Bunga Telang Sebagai Produk Bioteknologi Berupa Pupuk Cair Organik Dalam Menunjang Pertumbuhan Seledri Rezaldi, Firman; Kartina, Kartina; Susiyanti, Susiyanti; Maritha, Vevi; Susilo, Hadi
Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Vol 9 No 2 (2024): January 2024
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/e-jbst.v9i2.557

Abstract

Fertilizers produced from a mixture of materials that are easily decomposed, easily improve the physical, chemical and biological properties of the soil because they contain nutrients as an effort to increase plant growth are known as organic liquid fertilizers. The purpose of this study was to determine the potential of organic liquid fertilizer derived from the waste of butterfly pea kombucha fermentation combined with EM4 on the growth of celery plants. The research design includes T1, namely without treatment. T2 is the waste of butterfly pea flower kombucha fermentation added with 1 mL/L of EM4. T3 is the waste of butterfly pea flower kombucha fermentation added with 3 mL/L of EM4. T4 is the waste of butterfly pea flower kombucha fermentation added with 5 mL/L of EM4. The parameters of this study include plant height and number of leaves. The results of this study proved that kombucha fermented telang flower waste combined with EM4 correlated positively in supporting the growth parameters of celery plants, both plant height and number of celery plant leaves. The conclusion from this research is that the 5 mL/L treatment of 5 mL/L kombucha fermentation waste is the best treatment to support the research parameters, namely growth data on the average plant height and the number of leaves of celery plants.
Hubungan Malnutrisi Jumlah Total Albumin dan Protein Serum Terhadap Aktivitas Siswa Budiyanto, Ratno; Sumo, Maim0n; Prasetyo, Ferry Budi
Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Vol 9 No 2 (2024): January 2024
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/e-jbst.v9i2.559

Abstract

Albumin dan Protein merupakan salah satu tolak ukur terhadap penentuan malnutrisi terhadap status gizi manusia. Keduanya dapat mengidentifikasi apakah ada korelasi terhadap aktivitas siswa yang rutin sarapan pagi dan yang tidak. Penelitian ini bertujuan untuk menganalisis perbedaan tingkat konsentrasi siswa selama menempuh pendidikan dalam kelas yang diukur menggunakan observasional analitik desain cross-sectional dan kadar albumin dan protein serum siswa. Sebanyak 22 siswa skrining risiko malnutrisi menggunakan Subjective Global Assessment (SGA) dilanjutkan dengan wawancara. Kadar diambil dari serum siswa dan Analisis statistik menggunakan uji korelasi nonparametric test menggunakan SPSS 26. Terdapat perbedaan signifikan perubahan kadar albumin p=0.001 dan protein p=0.002 antara siswa yang sarapan pagi dan yang tidak (p<0.05). SGA mampu mendeteksi risiko malnutrisi pada 6 orang (27,3%) dan tidak berisiko manlutrisi pada 16 orang (48,4%), secara statistik albumin dan protein tidak signifikan berisiko malnutrisi dengan p-value 0.65 dan 0.70. Disimpulkan bahwa tidak ada hubungan bermakna antara siswa yang sering sarapan pagi dan tidak dengan kadar total protein dan albumin (p > 0.05), hanya saja ada penurunan konsentrasi pada saat kegiatan belajar mengajar terutama menjelang siang sekolah
Isolasi Dan Karakterisasi Bakteri Asam Laktat Dari Susu Kuda Bima (Equus sp.) Yang Berpotensi Sebagai Probiotik Suryani, Elsa Mega; Gaffar, Affan
Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Vol 9 No 2 (2024): January 2024
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/e-jbst.v9i2.561

Abstract

The aim of this research is to isolate and characterize lactic acid bacteria from mare's milk which have the potential to act as probiotics. Bacterial identification includes gram staining, antimicrobial activity test, hemolytic activity test, pH and bile salt tolerance test. The research results showed that C5 isolates isolated from horse milk samples had potential as probiotics. Characterization includes the form of bacillary bacteria, antimicrobial activity against the pathogenic bacteria Escherichia coli ATCC of 0.40 mm, gamma hemolysis, tolerance to pH 2, 3, 5 and tolerance to bile salts of 0.4-1%.

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