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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 2,125 Documents

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GEJALA KLINIS DAN PATOLOGI ANATOMI PASCA INFEKSI Campylobacter jejuni PADA AYAM BROILER [Clinical Symptoms and Pathology Anatomy due to Infection Campylobacter jejuni on Broilers] Andriani, Andriani; Sudarwanto, Mirnawati; Setiyaningsih, Surachmi; Kusumaningrum, Harsi Dewantari; Pisestyani, Herwin
BERITA BIOLOGI Vol 11, No 3 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Campylobacter spp. is a bacteria foodborne zoonotic agent that can infect humans and animals, especially birds. This bacteria can cause important problems in the public health since the beginning at the farm level, the level of retail and on the table. Poultry meat is the main source of contamination because the digestive tract of poultry is the predilection of Campylobacter jejuni. During this infection C. jejuni in poultry did not show typical clinical symptoms, making detection quite difficult to diagnose this disease at the farm level. Incidence infection of Campylobacter sp. in developed countries had 5-90%. Currently poultry production and consumption are predicted to still increase due to a high demand for low price protein. This research is conducted to determine the clinical and pathologic anatomy due to an infection of C. jejuni on chicken digestive tract. A total of 105-day-old chickens are divided into 3 groups, each consisting of 35 individuals. Group A was the control group (not infected) group B (infected with C. jejuni ATCC 33291), and group C (infected with field isolate of C. jejuni/C1). Infection of the chicken is done 0.5 ml peroral suspension concentration of104cfu/ml. Anova Duncan is used to analyze parameters of body weight, increase weight gain, feed consumption, FCR values. Kurskall Walis method used to analyze the parameters non-parametric form of the score changes in the macroscopic and microscopic intestinal and liver. Infection of C. jejuni changes the pathology anatomy of intestine i.e. hyperemia, enteritis and enteritis catharalis haemorrhagic. A microscopic change of intestinal is oedema,haemorrhage and infiltration of inflammatory cell. Changes of pathology anatomy liver are colour smeary, congesti, pale, fragile and necrotic. Microscopic changes of liver are degeneration and focal hepatic necrosis. Group observation infected by C. jejuni caused chicken cannot reach maximum body weight and can be detrimental to farmers because of increase of the value of the feed conversion ratio (FCR).

STRATIFIKASI SIMPANAN KARBON DIATAS PERMUKAAN TANAH PADA LAHAN GAMBUT PASANG SURUT DAN LEBAK [The Stratification of Above Ground C-Stock in Tidal Peatland and Fresh Water Swampland] Zakiah, Siti Nur; Wakhid, Nur; Nursyamsi, Dedi
BERITA BIOLOGI Vol 16, No 3 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The carbon stored in peatlands is huge not only from soil but also from vegetation. Carbon stocks can decrease when there are human activities such as land use changes. Measuring and monitoring carbon stocks are necessary as the basis for assessment of the impact of land management technology applications to conservation and carbon emissions associated with sustainable management system of peatland. The purpose of this study was to determine the stratification of above ground C-stock in tidal peatland and fresh water swampland. Above ground C-stock stratification based on the types of vegetation. The stratification was conducted to distinguish vegetation conditions based on the volume of biomass and carbon content in an observation plot. The measurement of above ground C-stock was carried out by destructive and non destructive refers to Hairiah K and Rahayu (2007), after that the estimation of carbon stockswas conducted on tidal peatland (land use rubber + pineapple, rubber folk and shrubs) and peat in fresh water swampland (land use rubber 4-5 years and 2-3 years). The results showed that the types of vegetation, plant density and management affect of carbon stocks. Carbon stocks in tree vegetation are higher than shrubs. The high of plant density affects the sunlight used for photosynthesis, through photosynthesis, CO2 is absorbed and converted by plants into organic carbon in the form of biomass. Arrangement and maintenance of the plant affects the storage of carbon in a land use.

PENGARUH FORMALIN DAN pH TERHADAP DAYA HIDUP SPERMATOZOA SAPI YANG DISIMPAN PADA SUHU RUANGAN MAUPUN 5Â°C Situmorang, Polmer; Triwulanningsih, E; Sugiarti, T; Kusumaningrum, DA; Sianturi, RG
BERITA BIOLOGI Vol 7, No 3 (2004)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The study was conducted in Research Institute for Animal Production (RIAP)-Ciawi. Semen was collected from 3 bulls twice a week by artificial vagina (AV), diluted in Tris buffer (control) and containing of 0.0625% formalin, reducing pH to 5.5 or their combination to temporally inhibit the motility of sperm.Diluted semen were store at ambient temperature for 0, 3 and 24 hours or at 5Â°C for 0, 3 and 7 days.Reactivation of motility was performed by dilution of formalin and bring the pH to 7.0. In ambient temperature,formalin and pH significantly (p<0.01) inhibit the motility of sperm, but these inhibition was reversible. The percentage of motility was significantly higher(p<0.01) in formalin than in pH 5.5 or control, following 24 hours of storage periode. The inhibition effect of combination between formalin and pH was non permanent. It is show that the inhibition effect of treatments was permanent for semen stored at 5Â°C.

IDENTIFIKASI GEN PENYANDI PIREN DIOKSIGENASE PADA ISOLAT BAKTERI PENDEGRADASI PIREN Febria, FA; Jamsari, Jamsari; Nasir, N; Nurhidayat, N
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The pyrene dioxygenase coded by gene is an indicator of bacterial isolates capabilities in pyrene degradation. The encoded gene of pyrene dioxygenase can be amplified and detected in Pseudomonas sp. PyrA2 and Burkholdoriasp. PyrA4 isolates, using primary specific Diox which designed based on PhdF gene sequence, the coding gene of pyrene dioxygenase in Mycobacterium vanbalenii PYR-1. The sequence alignment of pyrene dioxygenase putative gene in both of bacterial isolates with the sequence of pyrene dioxygenase coding gene in M. vanbalenii PYR-1 shows the similarity percentage of 41 % and 42% with Pseudomonas sp. PyrA2 and Burkholdoria sp.PyRA4.

ANALISIS RAGAM GENOTIP RAPD DAN FENOTIP TRUSS MORFOMETRIK PADA TIGA POPULASI IKAN GABUS [Channastriata(Bloch, 1793)] [Analysis of Genotype Variation and Truss Morphometricof Three Populations of Snakehead Fish [Channa striata (Bloch, 1793)] Gustiano, Rudhy; Oktaviani, Tia; Soelistyowati, Dinar Tri; Kusmini, Irin Iriana; Wahyutomo, Wahyutomo; Huwoyon, Gleni Hasan
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

In order to manage genetic resources for aquaculture development of snakehead fishChannastriata(Bloch, 1793), genetic variability of three populations from different geographical areas is needed to be understood. The purpose of this study was to identify the genotype and fenotype of snakehead fish from Jawa, Sumatera and Kalimantan using RAPD and âTrussâ morphometric. RAPD method used OPA-02, OPA-04 and OPA-07 primers.While twenty one measurement of truss morphometric was done on the body of fish observed. The results showed that population from Java had higher percentage of polymorphism and heterozygosity than those of Sumatera and Kalimantan, accounted for 83.33% and 0.3655 respectively. Population from Kalimantan and Sumatera had the lowest genetic distance of 0.1170.Meanwhile,the highest genetic distance (0.1908) was observed between population from Kalimantan and Java.Interpopulation relation based on the similarity of truss morphometric population from Sumatera and Kalimantan was 50%. However, those populations had similarity of 24.96% with population from Java. Coefficient variation of morphometric data showed that variation of population from Kalimantan was higher than those of Jawa and Sumatera.

SUKSESI SEKUNDER PASCA TEBANG PILIH HUTAN PAMAH DIPTEROCARPS DIDESA TAILELEU, TAMAN NASIONAL PULAU SIBERUT, SUMATERA BARAT Simbolon, Herwint
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

A study on the tree diversity and secondary succession after selective logging of lowland mixed dipterocarps forest has been conducted at three sites (Mongakngai, Kaloat, and Taipah) of Taileleu Village, Siberut Island National Park, West Sumatra. Plot size at each Mongakngai, Kaloat and Taipah site was: 100 x 30m; 100 x 40m; and 100 x 50m,respectively. Tree plants with DBH of more than 10 cm at each site were 50, 47 and 42 (or 101 species in total), while small tree plants of 2-10cm in DBH were 78, 11 and 98 (or 203 species in total), respectively. The most dominant species were Ficus subcordata, Aphorosa sphaeridophora and Castanopsios rhamnifolia at each site, respectively. Based on the similarity indices, those sites were clustered. Species diversity and structure of the forest at each site were also discussed in relation to the natural succession of forest.

PENGETAHUAN LOKAL DAN PEMANFAATAN TUMBUHAN OLEH MASYARAKAT LOKAL PULAU KABAENA- SULAWESI TENGGARA [Local knowledge and Plant Utilization by Local People of Kabaena Island Southeast Celebes] Rahayu, Mulyati; Rugayah, Rugayah
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

An ethnobotanical research focusing on the local people Moronene of the Wumbu Buro village, eastern part of Kabaena island,South East Sulawesi, was carried out using semi structural interviews and field observation. The local people practiced a traditional agricultural system. The results indicated maize Zea mays L., rice Oryza sativa L. and some other vegetable plants becoming the first priority species planted before cultivating horticultural plants such as cashew Anacardium occidentale L., cacao Theobroma cacao L. and coconut Cocos nucifera L. A total of 65 plant species were used by the local people for their daily needs, such as for food, utensil, roof, waving, medicine & cosmetic purposes including a plant species used for harvesting the palm sugar.The indigenous knowledge owned by the local people in utilizing plant species is discussed.

STATUS INFEKSI VIRUS INFLUENZA PADA BEBERAPA SPESIES HEWAN SEBELUM WABAH AVIAN INFLUENZA H5N1 PADA UNGGAS DI INDONESIA Sendow, Indrawati; Adj id, RM Abdul; Selleck, Paul
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

After outbreak of Avian Influenza HPAI in chicken in mid 2003 in Indonesia, there was a question whether Avian Influenza HPAI was already presence in animals before the outbreak. A retrospective study was conducted to gain information on the presence of Influenza A virus infection in a range of animal species that could be infected by the virus. A total of 1529 animal sera, from 8 species from 12 different provinces which were stored at the Bbalitvet (Research Institute for Veterinary Science) Serum Bank were tested against matrix antigen of Influenza A using the Agar Gel Immunodiffusion (AGID) test. The results indicated that only 0.6% of animal tested which consisted of 4% of duck sera and 0.4% of pig sera were reacted in the AGID test with weak reaction. Those sera were then tested against Influenza A group viruses using an Enzyme Linked Immunosorbent Assay (ELISA), indicated that Influenza A viruses were not detected in either duck and pig positive sera. Those sera which were also tested by HI test against antigens of HI, H3 and H7, also indicated that none of those sera were reacted. In addition, 134 lung of pigs from an abattoir were collected for virus isolation. The viral isolation on chicken embryonated eggs resulted in 12 samples that contained viruses with agglutinated goose and chicken red blood cells. Identification of viruses isolated was done by agglutination test and ELISA. The results showed that none of those isolates were Influenza Type A virus. This study showed that influenza A virus group infection was not detected in animal species sampled before outbreak of AI H5N1 in 2003 in Indonesia.

PERBANYAKAN TANAMAN WIEP {Grevillea papuana) SECARA KULTUR JARINGAN [Tissue Culture of Wiep Plant {Grevilleapapuana)] Sukamto, L Agus
BERITA BIOLOGI Vol 6, No 5 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The population of wiep (Grevillea papuana) originated from Papua, decreased because of over cutting or over exploitation for timber, ornamental or medicine. Experiment was carried out to propagate wiep by using tissue culture method.Hypocotyls,cotyledons and nodes from seedlings grown in vitro, were used as explants. These explants were grown on Murashige & Skoog medium which macro and micronutrients were half strength, with or without hormones (BA 1 mg/1, BA 1 mg/1 + NAA 0,5 mg/1, BA2 mg/1 + NAA 0,5 mg/1, BA 1 mg/1 + 2,4-D 0,5 mg/1, and BA 2 mg/1 + 2,4-D 0,5 mg/1). All explants did not produce callus on medium without hormones, but produced callus with hormones except BA treatment on cotyledon explants. Bigger callus were produced by explants that were treated by combination BA with NAA or 2,4-D compare to those treated by BA only. Nodes and hypocotyls could produce shoots, but cotyledons did not. Three kinds of explants did not produce any roots. Node is the best explants for producing shoots. Combination of BA 1 mg/1 and 2,4-D 0,5 mg/1 caused synergistic effect to stimulate shoot production of wiep hypocotyls explants.

ANALISIS FRONT SALINITAS BERDASARKAN MUSIM DI PERAIRAN PANTAI BARAT SUMATERA [Analysis of Salinity Front by Season in the Coastal West of Sumatra] Supiyati, Supiyati; Suwarsono, Suwarsono; Astuti, Nissa
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Technology of the marine sciences, especially the potential Â resources of fish can be evalute from front area identification. The aim of this research is to identify front salinity area based on seasonal variation in the west coastal of Sumatra. The method involved modeling by ODV Â (Ocean Data View), Fortran, Transform, and Matlab software. We used salinity data what obtained from NOAA (National Oceanic and Atmospheric Administration). The result showed that vertically, front areas in coastal West of Sumatera for all off season was in depth of Â± 41.6 meters, 83.2 meters and 124.8 meters. In the North West Monsoon, salinity front area was around of the Nias, Simeulue, Northwest and southeast Â of Enggano islands. On the first of transition period was in West of Simeulue, southeast of Nias, and East Enggano Islands. In the South East Monsoon was in the Northeast of Simeulue, the East of Mentawai, Southeast and Northwest of Enggano Islands. On the second transition period was in the Eastern of Nias and Mentawai Islands. Salinity changes of fourth monsoons on this front area is Â± 0,5 psu.

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