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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 2,125 Documents
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PURIFIKASI DAN KARAKTERISASI ENZIM PEKTINASE DARI ASPERGILLUS USTUS BL5 Yopi, Yopi; Rahmani, Nanik; Andriani, Ade; Dewi, Fitria; Meryandini, Anja
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i3.646

Abstract

Pectinase is an enzyme that could hydrolyze pectin into galacturonic acid. Natural pectinase was produced by microbes such as bacteria, yeast, fungi and Actinomycetes. Application of pectinase in industry were mainly in juice industry, textile, pulp, tea, cocoa and coffee fermentation. In this research, we conducted purification and characterization of pectinase produced by Aspergillus ustus BL5 in submerged fermentation using commercial pectin. The result showed that the optimum of pectinase production was reached at 120 hours fermentation process with specific activity 0.59 U/mg. The crude extract of pectinase was then concentrated using PEG 6000 and purified by Sephadex G-75 gel filtration chromatography. There were 2 fractions contained pectinase which the activity was 4.15 U/mg (pectinase A) and 3.3 U/mg (pectinase B), respectively. Compare to crude extract, the yield product of pectinase A and B increased 6.94 and 5.53 times, respectively. The purified pectinase A have optimum temperature at 50 oC and optimun pH at 5.
INTERAKSI GALUR × LINGKUNGAN, POTENSI HASIL DAN STABILITAS HASIL GALUR HARAPAN KEDELAI {GLYCINE MAX (L.) MERR.} HITAM Adie, M Muchlish; Krisnawati, Ayda; Susanto, GWA
BERITA BIOLOGI Vol 12, No 1 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i1.520

Abstract

Recent demand of black soybean {Glycine max (L.) Merr.} seed has been rising for industrial raw material. A total of five promising lines of black soybean (9837/K-D-8-185, 9837/K-D-3-185-195, W/9837-D-6-220, 9837/K-D-3-185-82 and 9837/W-D-5-211) were evaluated for the potential yield at 18 soybean production centrals in West Java, East Java, Bali and West Nusa Tenggara, from 2004 until 2006..Variety of Cikuray (black soybean), Wilis (famous variety with high yield) and Burangrang (large seed size and short maturation day) were used as check varieties.The field experimental design for each location was completely randomized design with four replicates.The treatment consist of eight lines/variety with 2,4 m × 4,5 m plot size, 40 cm × 15 cm plant distance, two plants/hill.Fertilizer of 50 kg Urea, 100 kg SP36 and 75 kg KCl per ha were applied before sowing time. Weed, insect and disease were controlled intensively.Stability analysis was according to Eberhart and Russell (1966), and adaptability was by Finlay dan Wilkinson (1963). Location, genotype and interaction between 2genotype x environment (G × L) were significant for seed yield. Estimate of environment variance component (d L = 0.0604) was the 2 2 biggest one, and followed by error variance value (d E = 0.0470), genotype variance (d G = 0.0258) and the smallest was estimate of 2 interaction variance G × L (d GL = 0.0225).Significant interaction of G × L showed that each promising lines have specific reaction to certain location quality. Five promising lines were able to produce 2.44 until 2.51 t/ha, or 17% higher than black soybean variety of Cikuray (2.03 t/ha); or 10% higher than Burangrang and 3% more higher than Wilis. Genotype of 9837/K-D-3-185-195, W/9837-D-6-220, 9837/K-D-3-185-82 and 9837/W-D-5-211 were indentified stable. Genotype of 9837/K-D-3-185-195, W/9837-D-6-220, 9837/K-D-3-185-82 and 9837/W-D-5-211 were promising to be high yielded and adaptable across location; on the contrary, genotype of 9837/K-D-8-185 was more adaptable in optimal location.
FUNGI MIKORIZA ARBUSKULA PADA RIZOSFER PERICOPSIS MOONIANA (THW.) THW. DI SULAWESI TENGGARA Husna, Husna; Budi R, Sri Wilarso; Mansur, Irdika; Kusmana, Cecep; Kramadibrata, Kartini
BERITA BIOLOGI Vol 13, No 3 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i3.670

Abstract

The research was conducted with the aim to identify species of arbuscular mycorrhizal fungi (AMF) associated with nedum [Pericopsismooniana (Thw.) Thw.]. Soil sampling was conducted in 6 locations of nedum habitat in South-east Sulawesi. The results showed that nedum associated with 15 species of AMF that belonging to 5 families and 9 genera, Acaulosporaceae (Acaulospora scrobiculata and A. delicata), Claroideoglomeraceae (Claroideoglomus etunicatum), Glomeraceae (Glomus aggregatum, G. boreale, G. canadense, G. halonatum,G. versiforme, Rhizophagus diaphanus, R. fasciculatus, Sclerocystis clavispora and Septoglomus constrictum), Gigasporaceae (Racocetragregaria and Scutellospora auriglobosa), Ambisporaceae (Ambispora appendicula). The four species of AMF were found on nedum, i.e Glomus boreale, G. canadense, G. halonatum and Racocetra gregaria which were a new record for mycological collection of Indonesia.
PENGUJIAN PENCEMARAN DAGING BABI PADA BEBERAPA PRODUK BAKSO DENGAN TEKNOLOGI PCR: PENCARIAN SISTEM PENGUJIAN EFEKTIF Margawati, Endang Tri; Ridwan, Muhamad
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i1.2055

Abstract

Entering globalization market, Indonesian government could not reject any import of food products from overseas. To anticipate the possibility of porcine contaminants into processed meat products of imported food such as meat or chicken ball, sausage, tin meat etc., it is important to apply laboratory research on such particular matter in regard to ethical and certain religious concern. This study was intended to identify the possibility of porcine contaminants into either processed meat products or fresh meat.A technique of polymerase chain reaction (PCR) was applied and PCR optimizing was conducted in advanced to obtain the right annealing temperature.Positive control of fresh pork meat was amplified to get porcine Leptin size which is 152bp fragment. Five samples of 4 meat balls and one fresh beef meat were individually collected for their DNA by either from minced or mashed after liquid nitrogen exposure then followed with a series of DNA extraction steps. PCR was assigned by using a specific primer of Leptin gene for porcine identification.Visualization of Leptin fragment was applied either on 1%, 2% of agarose gel or 10-20% gradient acrylamide gel.The result showed that all sample applied were not identified for containing porcine contaminants while positive control was on the right size of 152bp of Leptin gene. Specific primer used in this study was proved that there was not identified porcine Leptin gene on the negative control (fresh beef meat). This study suggests that a method of PCR is a simple analytical method for identification of porcine contaminants and visualization on 2% agarose gel is a cheaper and quicker method while by gradient acrylamide gel showing more clear band however this method is time consuming and expensive.
PERSPEKTIF GENDER SUKU OSING DI BANYUWANGI DALAM PENILAIAN KEMANFAATAN TANAMAN Prasetyo, Budi; Chikmawati, Tatik; Walujo, Eko Baroto; Zuhud, Ervizal A.M
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.3689

Abstract

The different roles and responsibilities of male and female of the Osing tribe influence the knowledge of the use of plants. The aims of research is to analyze the values of the benefits of plants based on gender perception. The research used a qualitative and quantitative methods. Data collection for the value of plant species utilization were carried out  using Pebble Distribution Method (PDM) and analysis used Index of Cultural Significance (ICS). The Osing community recognised 86 plant species that were grouped in to eight beneficial groups. The male in three villages have a better understanding of ethnobotanical knowledge and practice than female on crops as staple foods, medicines, fruits, and firewood, while understanding of ornamental plants are relatively the same for both male and female. Female have a broader and more detailed understanding on vegetables, plant species used as building construction, and traditional rituals. The high PDM value in eight beneficial groups was supported by the high value of each ICS.  
KERAGAMAN KERAPATAN KAYU BATANG DAN CABANG KOMUNITAS POHON DI HUTAN GUNUNG PAPANDAYAN, JAWA BARAT Tihurua, Eka Fatmawati; Sulistyawati, Endah
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.3519

Abstract

Wood density is the functional character which has important role in the function of ecosystem. Stem and branch wood density have different trait and effect on its ecosystem processes. The objectives of this research are to know the diversity of stem and branch wood density and to analyze whether branch wood density could be used to estimate stem wood density in the Mount Papandayan. Six plots of 0.1 ha at different sites (three plots each in interior and edge forest) were established. Branches were collected from trees with diameter at breast height larger than 10 cm, while stem wood density data were obtained from some wood density sources. Research results showed that stem wood density ranges were 0.35?0.82 g/cm3, while branch wood density ranges were 0.33?0.61 g/cm3. Average of branch wood density was lower (0.48 ± 0.09 g/cm3) than stem wood density (0.61 ± 0.1 g/cm3). Linear regression analysis indicated that branch wood density could be used to estimate stem wood density of trees in Mount Papandayan which is showed by R2 value and correlation coefficient of 0.28 and 0.55 (p value < 0.001) respectively. 
KARAKTERISASI REPRODUKSI DAN NILAI HETEROSIS HASIL PERSILANGAN IKAN GURAME BASTAR DAN BLUESAFIR Radona, Deni; Nafiqoh, Nunak
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.689

Abstract

Hybridization is one alternative of genetic improvement to increase the production of commercialize commodity. The aim of this study was to determine the character of reproductive performance and the value of heterosis from cross-breeding of giant gouramy (Osphronemus gouramy) strain Bastar and Bluesafir. Crosses were performed in both directions (reciprocal) with naturally spawning system. Two pairs of giant gouramy of each strain were used, each pair contained 4 ? and 1 ?. Every pairs were reared in 3 x 3 m of pond.Reproductive characterization, such as Fecundity, Ovo Somatic Index, egg diameter, egg weight, Fertilization Rate (FR), Hatching Rate (HR), the latency time of hatching, and yolk sack absorbtion period, were observed. Other parameters including larval lenght and Survival Rate (SR) were also observed. Heterosis (H) value was calculated from FR, HR and SR. Result showed that each population and its cross-breed had no different in reproductive characters. Latency time of hatching, egg diameter, egg weight and yolk sac absorbtion period values were 3 ± 0 days; 0.74 ± 0.05 cm; 11 ± 4 mg dan 9 ± 1 days, respectively. Meanwhile fecundity value showed that Blusafir strain had higher fecundity (3661 ± 624), and Ovo Somatic Index percentage (21.96 ± 3.74). The highest HR was presented by ? Bluesafir x ? Bastar (97.95 ± 0.47 %), meanwhile ? Bastar x ? Bluesafir presented the highest FR and SR (99.15 ± 0.34 % and 92.57 ± 0.40 %). Heterosis value showed that cross-breeding between ? Bastar x ? Bluesafir had better HR and SR (1.20 and 2.37), white hand cross-breeding between ? Bluesafir x ? Bastar presented better FR (0.69).
KEANEKARAGAMAN KOMUNITAS KRUSTASEA DI KEPULAUAN MATASIRI KALIMANTAN SELATAN Pratiwi, Rianta; Wijaya, Nirmalasari Idha
BERITA BIOLOGI Vol 12, No 1 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i1.525

Abstract

Studies on the community diversity of crustaceans have been conducted in the waters of Matasiri Islands, South Kalimantan, from 19th November to 1st December 2010. This research was conducted to determine the density, diversity and the presence of crustacean fauna in the waters of South Kalimantan, especially in the Matasiri Islands. Samples were collected using trawl gear operated by KR (Research Ship) BarunaJaya VIII in four Stations and three Stations of free collecting along the islands. Free sampling was conducted along the beach and the reef edge by breaking live and dead rocks, and digging sand and mud in the mangrove or using hand net around the beach of Matasiri Islands. Collection is also done using the gillnet gear which is installed in shallow water during high tides about 4 hours.The samples were collected during low tide. The results obtained were 1882 individuals covering 86 species and 19 families. The diversity index ranges between 0.97 (the lowest at Station 3 of free collecting) and 3.74 (the highest in Station 3 of trawl). While the similarity index ranged from 0.36 (the lowest in Station 1 of trawl) to 0.97 (the highest in Station 3 of free collecting). Penaeidae prawn has the highest density in each observed station. Portunidae crab has the second highest density. South Kalimantan waters especially Matasiri Islands is still in good condition for crustacean life.
LUMUT SEJATI DI KAWASAN CAGAR ALAM GUNUNG PAPANDAYAN GARUT, JAWA BARAT Windadri, Florentina Indah
BERITA BIOLOGI Vol 13, No 3 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i3.675

Abstract

Research on moss diversity in the Papandayan Nature Reserve has been conducted covering the western and eastern areas of the Nature Reserve. Fifty one species of mosses from 14 families and 30 genera were recorded and collected with Dicranaceae as the dominant family.Four species of mosses (Papillaria crocea (Hampe) Jaeg., Barbella rufifolioides (Broth.) Broth., Ctenidium luzonense Broth. and Trichostomum brachydontium Bruch ex Mull.Hal are reported here as new records to Java.
IDENTIFICATION AND PATHOGENICITY TEST OF SOME BACTERIA ISOLATED FROM WILD AND FARMED SPINY LOBSTER PANULIRUS HOMARUS Sudewi, Sudewi; Widiastuti, Zeny; Mastuti, Indah; Mahardika, Ketut
BERITA BIOLOGI Vol 18, No 3 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i3.3578

Abstract

The bacterial populations in the farming of spiny lobster could have either beneficial or harmful effects depending on the prevailing conditions. We designed this study to identify and to perform a pathogenicity test of some bacteria isolated from wild and farmed spiny lobsters Panulirus homarus. The adult farmed lobsters were obtained from Pangandaran and Lombok coastal areas, while the wild lobsters were collected from Lombok, with five lobsters for each location. The bacteria were isolated from the midgut, gill, hepatopancreas, and muscle tissues of the lobsters. The identification of the bacteria was carried out by molecular methods. Pathogenicity test was performed by intramuscular injection of 0.1 ml bacterial suspensions at the density of 7×106cfu/ml into each three adult apparently healthy lobsters for every eight bacterial isolates. Our study identified six bacterial isolates that exhibited high homology of a nucleotide sequence with Shewanella algae, Bacillus firmus, Vibrio alginolyticus, Tenacibaculum lutimaris, Pseudomonas sp. and Vibrio sp., while two isolates were reminded unidentified due to low nucleotide similarities (< 97%). The pathogenicity test showed that there was no mortality of lobsters injected with those bacterial isolates. This may because the dose of injection was too low to induce bacterial infection particularly for Vibrio, or the bacteria were not pathogenic for lobster or even have the potency as probiotic bacteria.   

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