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Majalah Obstetri dan Ginekologi

MOG
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Published by Universitas Airlangga

ISSN : 08540381 EISSN : 25981013 DOI : 10.20473/mog.V27I32019.90-93

Core Subject : Health,

Health Professions Medicine & Pharmacology

Majalah Obstetri & Ginekologi (MOG) or the Journal of Obstetrics & Gynecology Science is a scientific journal published by the Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia, in collaboration with the Indonesian Obstetrics and Gynecology Association (POGI) of Surabaya branch.

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Articles 286 Documents

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Penurunan progesterone-induced blocking factor (PIBF) sebagai penanda preeklampsia Achmad Salman; Budi Wicaksono; Erry Gumilar Dachlan; Widjiati Widjiati
Majalah Obstetri dan Ginekologi Vol. 24 No. 1 (2016): Januari - April
Publisher : Universitas Airlangga

Background: Pathogenesis of preeclampsia still a controversial research objects. Progesterone induced blocking factor has protective effects in pregnancy.Objectives: To compare PIBF serum level and placental weight and investigate correlation between both in preeclampsia.Methods: This experimental study used 16 Mus musculus with normal pregnancy and 16 Mus musculus that were injected with anti Qa-2 to create a preeclampsia model. Terminations of were performed in day 16th, followed by ELISA examination for PIBF serum level and placental weight measurement.Result: PIBF serum level significantly reduced on preeclampsia model compared with control (33,21±29,36 ng/ml vs 105,76±59,69 ng/ml; p<0,05). There was no different of placental weight in preeclampsia model compared with control (97,75±50,74 mg vs 116,09±44,45 mg; p>0,05). There was no significant correlation between PIBF serum level and placental weight.Conclusion: Decreased of PIBF serum level could be used as a marker of preeclampsia. Decreased of PIBF serum level may be correlate with placental pathology in preeclampsia.

Nutritional status of third trimester pregnant women correlates positively with birth weight Ratna Ernawati; Bangun Trapsila Purwaka; Budi Prasetyo
Majalah Obstetri dan Ginekologi Vol. 25 No. 2 (2017): August
Publisher : Universitas Airlangga

Objectives: to identify correlation between nutritional status (energy, macronutrient and fluid intake) of the third trimester pregnant women and birth weight.Materials and Methods: correlative analytic prospective cohort’s study in Public Health Center Jagir, Surabaya with sample comprising healthy pregnant women aged 20-35 years in the third trimester and their newborns (n=34). Respondents noted fluid intake every day for 7 days (record method) and food for 24 hours (recall method). Adequacy of energy intake, macronutrient and fluid (mean) was assessed based on AKG 2012. LBW when <2500 g. Statistical analysis used Pearson correlation's and Spearman's rho with test with significance level of p<0.05.Results: in third trimester pregnant women energy intake (r: 0.854, p: 0.0001) and carbohydrates (r:0.912, p:0.0001) had strong positive correlation, protein intake had moderate positive correlation (r: 0.519, p: 0.0001), fat intake (r: 0.425p: 0.012) and fluid (r: 0.469 p: 0.005) had positive correlation with birth weight. The prevalence of LBW in Public Health Center Jagir Surabaya was 14.7%.Conclusion: Nutritional status of third trimester pregnant women correlates positively with birth weight.

The awareness of urinary tract infection management in pregnant women. A qualitative study Budi Iman Santoso; Raymond Surya; Farah Asyuri Yasmin; Rima Irwinda
Majalah Obstetri dan Ginekologi Vol. 25 No. 3 (2017): December
Publisher : Universitas Airlangga

Objectives: to identify knowledge, attitude, and practice of health providers including GPs and gynecologists to the implementation of the national guideline on the treatment of UTI among pregnant women.Materials and Methods: A cross-sectional study design was used. Subjects were women who attended the International Sym-posium of UTI. Questionnaires consisting ofregarding demo-graphic characteristics, knowledge, attitude, and practice were given to the subjects. The questionnaires had been tested for valid-ity and reliability by applying the Pearson correlation and Cronbach’s alpha test. Statistical analyses were performed using SPSS 23.0 for Windows.A two-tailed p value less than 0.05 was considered to be statistically significant.Results: A total of 140 subjects were recruited in this study. Of these, 104 subjects (74.3%) returned the questionnaire, and 99 subjects (70.7%) were eligible for this study. Nine (9.1%), 69 (69.7%), and 21(21.2%) subjects had good, fair, and poor know-ledge, respectively. Sixty-five (65.7%) and 64 subjects (64.6%) showed a positive attitude and had positive practice, respectively.Conclusion: Knowledge, attitude, and practice among respon-dents are good enough, despite only a few of them have read the updated guideline. Continuous medical education through online update or symposium may be one effective method to disseminate new update in guidelines.

Effect of oocyte vitrification before and after in vitro maturation towards Bcl-2, Bax and Bcl-2/Bax ratio expression Zakiyatul Faizah; R. Haryanto Aswin; Hamdani Lunardhi
Majalah Obstetri dan Ginekologi Vol. 24 No. 2 (2016): May - August
Publisher : Universitas Airlangga

Objectives: to compare the expression of Bcl-2, Bax and Bcl-2/Bax ratio in cumulus cell and oocyte between vitrified oocyte pre and post in vitro maturation.Materials and Methods: Maturation was operated in medium TC 100 µl for 24 hours. Vitrification begins with washing oocyte in PBS basic medium supplemented of 20% serum for 1-2 minutes, followed by equilibration medium PBS + 20% serum + 10% ethylene glycol for 10-14 minutes, then transferred to 20% serum + PBS + 0.5 M sucrose + 15% ethylene glycol + PROH 15% for 25-30 seconds. Thawing is processed by submerging the oocytes in the media: 1). PBS + 20% serum + 0.5 M sucrose, 2). PBS + 20% serum + 0.25 M sucrose, and 3). PBS + 20% serum + 0.1 M sucrose. Imunocytochemistry observed the expression of Bcl-2, bax and Bcl-2/bax ratio.Results: Bcl-2 expression on oocyte in control group differed significantly with treatment group, Bcl-2 expression on cumulus in control group differed significantly with treatment 1 group. Bax expression on oocyte in control group differed significantly with treatment group. Bax expression on cumulus in control group differed significantly with treatment group. Bcl-2/Bax expression ratio on oocyte and cumulus did not differ significantly in all groupConclusion: No difference Bcl-2/Bax expression ratio on oocyte and cumulus between vitrified oocyte pre and post in vitro maturation.

The comparison of creatinine and cystatin C value in preeclampsia severity and neonatal outcome John Wantania; Abraham Winarto
Majalah Obstetri dan Ginekologi Vol. 24 No. 3 (2016): September - December
Publisher : Universitas Airlangga

Objectives: to compare the levels of creatinine and cystatin C with the severity of preeclampsia, and assess neonatal outcomes.Materials and Methods: Creatinine, cystatin C, and neonatal outcomes were assesed in 17 normotensive samples, 17 samples of mild preeclampsia and 17 samples of severe preeclampsia. Analysis of data with statistical tests of ANOVA and t test differences between 2 proportions.Results: The mean levels of creatinine in the normotensive group, mild preeclampsia, severe preeclampsia are 0.56 mg/dL, 0.67 mg/ dL, and 0.75 mg/dL, p=0.138; While on cystatin C are 0.82 mg/L, 1.03 mg/L and 1.32 mg/L, p=0.000. The adverse neonatal out-come wasn’t found in the normotensive group. In mild pre-eclampsia obtained 1 preterm birth and 1 intrauterine fetal death (IUFD), whereas in severe preeclampsia obtained 3 babies born preterm, 1 IUFD, and 1 intrauterine growth restriction (IUGR).Conclusion: levels of cystatin C was increased significantly in line with increased severity of preeclampsia, whereas creatinine was not increased significantly. Cystatin C is better than crea-tinine as a marker of renal dysfunction in preeclampsia patients. There was an increase in adverse neonatal outcomes in the group of preeclampsia.

Pengaruh Monoklonal Antibodi Bovine Zona Pelusida 3 (bZP3) terhadap Diameter dan Atresia Folikel Ovarium Mencit (Mus musculus) Annisa Trissatharra; Sri Ratna Dwiningsih; Ratna Sofaria Munir
Majalah Obstetri dan Ginekologi Vol. 24 No. 1 (2016): Januari - April
Publisher : Universitas Airlangga

Objectives: To identify the effect of monoclonal antibody bZP3 at ovarian follicles that undergo atresia and diameter of various ovarian follicles.Materials and Methods: This is a true experimental research with post only control group design. Samples were 36 female mices (Mus musculus) which is divided into 6 groups, there are 3 control groups (group 1, 2, and 3) injected by Phospatase Buffer Saline (PBS) 50µl and 3 treatment groups (group 4, 5, and 6) injected by Mab bZP3 50µl. Group 1 and 4 terminated at 5th day, group 2 and 5 terminated at 10th day, and group 3 and 6 terminated at 20th day. Evaluation of atretic ovarian follicles and diameter of ovarian follicles performed by hematoxylin eosin (HE) and the data processed by parametric statistic.Results: There are no significant in different among groups in the aspect of atretic follicles and diameter of folicles (p>0.05), but descriptively, number of follicles undergo atresia of the follicle primary, secondary, and tertiary treatment group was higher than the control group, except on the 20th day of observation time.Conclusion: administration of Mab bZP3 had no effect to amount of atretic follicles and diameter of folicles during observation time.

Oxytocin massage enhanced breast milk production in post-partum women Widia, Lidia; Meihartati, Tuti
Majalah Obstetri & Ginekologi Vol. 25 No. 2 (2017): August
Publisher : Universitas Airlangga

Objectives: To evaluate the effect of oxytocin massage on breast milk production and maternal comfort in postpartum women. Materials and Methods: This analytical cross-sectional study utilized primary data collected through a structured questionnaire. A total of 36 postpartum mothers were recruited using a non-probability sampling technique. Data were analyzed using the Chi-square test with a significance level set at 0.05. Results: The Chi-square analysis demonstrated a significant association between oxytocin massage and breast milk production (p = 0.001). Conclusion: Oxytocin massage significantly improved breast milk production among postpartum mothers at the Batulicin 1 Public Health Center, Tanah Bumbu District, South Kalimantan.

Human pappilomavirus genotype in cervical tissue of patients with Cervical Intraepithelial Neoplasia (CIN) 1, CIN 2, and CIN 3 Gondo Mastutik; Rahmi Alia; Alphania Rahniayu; Anny Setijo Rahaju; Renny I’tishom; Suhartono Taat Putra
Majalah Obstetri dan Ginekologi Vol. 24 No. 3 (2016): September - December
Publisher : Universitas Airlangga

Objectives: to determine the genotype of HPV in patients with precancerous lesions of cervical tissue.Materials and Methods: An observational study with cross sectional study of patients paraffin block CIN1, CIN2, CIN3 was conducted in Dr Soetomo Hospital. HPV DNA was extracted from paraffin blocks, then performed PCR and genotyping of HPV. The sample consisted of 28 patients with cervical tissue paraffin blocks CIN1, CIN2 and CIN3. Patients aged between 26-74 years (standard deviation 10,12).Results: HPV genotypes that infect patients with CIN1 were HPV16 and 18, CIN2 were HPV16 and 52 and CIN3 were HPV16, 67, and combined infection HPV16/67 and HPV52/67. HPV genotypes in a single infection were 26/28 (HPV16, HPV18, HPV52 and HPV67), and multiple infections were 2/28 (HPV16/67 and HPV52/67).Conclusion: The most dominant HPV genotypes infect patients with precancerous lesions of the cervix were HPV16, HPV67, HPV52, and HPV18.

VEGF 121 Rekombinan Dapat Memperbaiki Endoteliosis Gromerular pada Mencit Bunting Model Preeeklampsia Sri Sulistyowati; Donny Irawan; Eric Edwin; Soetrisno Soetrisno
Majalah Obstetri dan Ginekologi Vol. 24 No. 1 (2016): Januari - April
Publisher : Universitas Airlangga

Preeklampsia masih merupakan penyebab utama morbiditas dan mortalitas maternal maupun perinatal. Pada preeklampsia terjadi gangguan ginjal berupa endoteliosis glomerular. Ketidakseimbangan faktor proangiogenik VEGF dan antiangiogenik sFlt-1 diduga terlibat dalam patogenesis preeklampsia. Pemberian VEGF 121 rekombinan memiliki kemampuan untuk memperbaiki fungsi endotel sehingga dapat memperbaiki endoteliosis glomerular. Penelitian ini bertujuan untuk membuktikan pengaruh VEGF 121 rekombinan terhadap perbaikan endoteliosis glomerular pada mencit model preeklampsia menggunakan metode eksperimental analitik dengan RCT, yang dilakukan pada bulan Januari – Mei 2015 di Kandang Hewan Percobaan dan Laboratorium Biomedik Fakultas Kedokteran Hewan Universitas Airlangga. Sampel dari replikasi Steel dan Torrie adalah 30 mencit bunting yang terbagi dalam 3 kelompok yaitu 10 mencit bunting normal, 10 mencit bunting model preeklampsia dan 10 mencit bunting model preeklampsia dengan terapi VEGF 121 rekombinan. Semua sampel dilakukan pemeriksaan pada endotel ginjalnya dengan metode imunohistokimia. Parameter endoteliosis glomerular menggunakan kriteria Stillman (2007) dan Wide-Swensson (2007). Analisa data menggunakan uji Kruskal Wallis dan Mann Whitney. Rerata endoteliosis gromeruler pada mencit bunting normal 1,60 dan mencit bunting model preeklampsia 1,90 dengan nilai p=0,28 (p>0,05). Rerata endoteliosis gromeruler pada mencit bunting model preeklampsia 1,90 dan mencit bunting model preeklampsia dengan terapi VEGF rekombinan 121 adalah 1,30 dengan nilai p=0,02 (p<0,05). Rerata endoteliosis gromeruler pada mencit bunting normal 1,60 dan mencit bunting model preeklampsia dengan terapi VEGF rekombinan 121 adalah 1,30 dengan nilai p=0,28 (p>0,05). Simpulan, VEGF 121 rekombinan dapat memperbaiki endoteliosis gromerular pada mencit bunting model preeeklampsia.

Inadequate fluid intake can increase the risk of constipation among pregnant women Dina Fitriana; Budi Prasetyo; Bangun Trapsila Purwaka
Majalah Obstetri dan Ginekologi Vol. 25 No. 2 (2017): August
Publisher : Universitas Airlangga

Objectives: To analyze the association between the fiber and fluid consumption with constipation in pregnancy.Materials and Methods: An analytic cross-sectional study conducted at Jagir Public Health Center Surabaya, with the samples of healthy pregnant women aged between 20-35 years old (n=99). Respondents were asked to record the amount of daily fluid intake, defecation frequency, and type of faeces, everyday for 7 days, also made a food recall during 24 hours. The recom-mendation of adequate fluid intake in first trimester was 2180 ml/day and 2300 ml/day for second and third trimester. The value of adequate fiber consumption was 25-30 g/day. Constipation diagnosed by the frequency of defecation <2x/week, or based on Bristol Stool Form Scale included as type 1, type 2, or type 3.Results: Pregnant women whose adequate fluid intake was 40,4% and those with adequate fiber intake was 4.1%. The percentage of constipation among them was 29.5%. There was significant association between fluid intake and constipation (p: 0,000; RR:1,85). There was no significant association between fiber intake and constipation p:0,317 (p<0.05). There was also no significant association between parity, gestational age, and level of education with constipation (p<0.05).Conclusion: Inadequate maternal fluid intake can increase the risk of constipation by 1.85 folds, and the adequate fluid intake may prevent constipation during pregnancy.

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