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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
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Isolasi dan Identifikasi Spesies Bakteri Asam Laktat Penghasil Senyawa Antimikrob Asal Kolon Sapi Bali (ISOLATION AND IDENTIFICATION OF LACTIC ACID BACTERIA SPECIES PRODUCING ANTI-MICROBIAL SUBSTANCE ISOLATED FROM COLON OF BALI CATTLE) Sri Anggreni Lindawati; I Wayan Suardana
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Bali cattle as one of the local cattle are known have uniquely genetic characteristics. They are easy toadapt at a less favorable environment, so that they are known as a pioneer cattle. According to theiruniquely, it may allow for the discovery of specific types of acid lactic bacteria compared with others. Thishypothesis is based on the assumption that the types of bacteria as a normal flora in the intestine tract ofcattle are highly dependent on several factors, and one of which is a feed factor. Based on the abovebackground, this study was conducted. The aim of study was to isolate and identify of a specific species oflactic acid bacteria that has anti-microbial substances. The study began by isolation of acid lacticbacteria originated from 20 fecal samples of colon of bali cattle through the growth on selective medium i.e.deMann, Rogosa, Sharpe (MRS) medium followed by Gram staining and catalase test. The screening ofantimicrobial activity of each isolate was performed by culturing of isolates again indicator bacterial on blood agar medium. The selected isolates were continuously tested on medium contains 15% NaCl ,medium with ph 9.6, and medium with temperature 10°C, respectively in order to identification genus ofbacteria. The final stage of identification in order to know the specific isolate, which has antimicrobialsubstances, was confirmated by using the API 50 CHL kit. The results of study showed that one of theisolates that known have widely antimicrobial activities was isolate with 3A code. This isolate hasinhibitory zone to indicator bacterial i.e. Staphylococcus aureus ATCC 29213 and Escherichia coli ATCC25922. This isolate is known as Lactococcus lactis ssp lactis 1 with its similarity value 65.7%. This isolateis potentially to continuously study in order to know the potency of isolate as a probiotic candidate and oras a food preservative.
Phenotypic and Serotypic Characterization of Staphylococcus aureus Strains from Subclinical Mastitis Cattle (KARAKTERISASI SECARA FENOTIPE DAN SEROTIPE STAPHYLOCOCCUS AUREUS YANG BERASAL DARI MASTITIS SUBKLINIK PADA SAPI) Siti Gusti Ningrum; Wyanda Arnafia; Sylvia Oscarina; Retno Damajanti Soejoedono; Hadri Latif; Mohammad Ashraf; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Staphylococcus aureus is known as a major causative agent of mastitis in dairy cattle. In the presentstudy, 104 isolates of Staphylococcus originated from subclinical mastitis cattle characterized for thephenotypic properties and the presence of Staphylococcal protein A (Spa). Some bacteria were resistancesagainst several antibiotics were also studied, such as erythromycin, streptomycin, tetracycline, cefepime,nitrofurantoin, amikacin, chloramphenicol, and ciprofloxacin. About 78% of the isolated were moderatelysensitive to nitrofurantoin, while 89% were highly resistant to cefepime and ciprofloxacin. Using thevarious mammals’ sera, seven isolates out of 104 revealed the presence of Spa.
Karakterisasi Morfometrik dan Jarak Genetik Rumpun-Rumpun Kelinci di Jawa Barat (MORPHOMETRIC CHARACTERIZATION AND GENETIC DISTANCE OF RABBIT BREEDS IN WEST JAVA) Rudi Dedi Iskandar; Bram Brahmantiyo; Rudi Priyanto
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objectives of this study were to assess morphometric characteristics, breeds relationship andvariables that distinguished among breeds of rabbits raised in West Java. This research used 419 rabbitsconsisted of Angora (AG), Dutch (DT), Flemish Giant (FG), Lop (LP), Netherland Dwarf (ND), Composite(PX), Rex (RX), Satin (ST), Reza (XA) and New Zealand White (ZW). Head length (PK), head width (LK), earlength (PTL), ear width (LTL), chest width (LD), chest depth (DD), chest circumference (LKD), body length(PB ), hips width (LP), length of the scapula bone (PS), humerus length (PH), radius-ulna length (PRU),femur length (PF) and tibia length (PT) were observed. Data were analyzed with analysis of variance,discriminant and canonical analysis using SAS program ver. 9.1.3 and MEGA5 program to get theconstruction of phenogram tree. FG and ST rabbits were generally larger in size and shape than the otherrabbits breeds, while ND rabbit had the smallest morphological size than other rabbits breeds, except forLK, LD and DD. Results of discriminant analysis showed that LP, RX, ND and XA had a high similarityvalue, otherwise DT, FG, ST, PX, AG and ZW had no the value. The closest genetic distance matrix valueindicated by PX-ZW breeds (1,53) and the farthest genetic distance indicated by FG-ND breeds (6,62).Phenogram tree construction showed that the breeds rabbits divided into five clusters, namely cluster ND,DT; ST clusters; FG cluster; cluster LP, PX, ZW and cluster AG, XA, RX. Phenotypic size that had stronginfluence on the differentiation of rabbit breeds were PTL, LTL, PRU, PH and PF on the canonical 1 alsoPT and PS on canonical 2.
PRODUCTION OF EMBBRYONIC STEM CELLS FROM INNER CELL MASS OF BLASTOCYST ISOLATED BY ENZYMATIC AND IMMUNOSURGERY METHODS Thomas Mata Hine; Iman Supriatna; Dondin Sajuthi; Arief Boediono
Jurnal Veteriner Vol 9 No 1 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of the research is determining the ICM isolation method to produce ESC. Blastocyst stage of DDy mice embryos were used in this study. Zona pellucida of blastocysts were removed by 0.25% pronase, the ICM isolation were done by enzimatic or immunosurgery method, and then they were cultured in DMEM-high glucose supplemented with mercaptoethanol, gentamycin, fetal bovine serum, and cumulus cells as feeder layer. The result of the research indicated that immunosurgery method yielding attachment rate and number ESC colony 93.85% and 43.08%, respectively, higher (P<0.05) than enzimatic method that weree 79.63% and 18.52%, respectively, but the viability of ICM cells were equal (P >0.05) that are 93.59% in enzymatic method and 98.56% in immunosurgery method. This research concluded that immunosurgery more effective method for isolation of ICM and ESC production than enzymatic method.
Aktivitas Antibakteri Ekstrak Jeruju (Acanthus ilicifolius) terhadap Pertumbuhan Vibrio harveyi Secara in vitro (ANTIBACTERIAL ACTIVITY OF JERUJU (ANACTHUS ILICIFOLIUS) EXTRACTS ON THE GROWTH OF VIBRIO HARVEYI IN VITRO) Gina Saptiani; Slamet Budi Prayitno; Sutrisno Anggoro
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this research was to study the potential of jeruju (Acanthus ilicifolius) extract as anantibacterial for alternative therapy and control of bacterial diseases in prawn nurseries. Ethanol extractionwas prepared from jeruju’s leaves, trunks, fruits, and flowers..Each extract was prepared at differentconcentrations (50 ppm, 100 ppm, 200 ppm, 300 ppm, 400 ppm, 500 ppm, 600 ppm, 700 ppm, 800 ppm,900 ppm, and 1000 ppm, respectively) and further tested its antibacterial activity against Vibrio harveyiusing the agar disc diffusion method. The results showed that A. ilicifolius is a potential antibacterial,extract of the leaves seemed to be more effective in inhibit the growth of V. harveyi compared to other partsof the plant.
Maltosa Mempertahankan Viabilitas Spermatozoa Epididimis Kerbau Belang yang Disimpan dalam Bentuk Cair Yulnawati -; Hera Maheshwari; Muhammad Rizal; Herdis -
Jurnal Veteriner Vol 11 No 2 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study to determine the effect of maltose supplement in andromed based semen extender on theviability of buffalo’s epididymal spermatozoa following 12 and 24 hour storage as liquid semen. Epididymalspermatozoa were collected by combined methods of slicing, flushing and tissue pressure. The spermatozoawas then diluted with three different extenders, i.e Andromed only (A) as control, Andromed + 0.2% w/vMaltose (M1) and Andromed + 0.4% w/v Maltose (M2) as treatments. The percentages of progressivelymotil spermatozoa in the liquid semen in the following treatment of A, M1 and M2 were 48.33%; 53.33%and 55% respectively (after 12h of storage), and 45%; 46.67% dan 45% respectively (after 24h of storage).Besides, the percentage of live spermatozoa in A, M1 and M2 after 12h of storage were 70.33%; 72 % and72.33 % and after 24h of storage were 66.33%; 70% and 70.67 %. In conclusion, the addition of 0.2% and0.4% w/v maltose into Andromed extender could maintain the life of the spotted buffalo epididymalspermatozoa after storage for 4°C up to 24 hours.
Hitung Diferensial dan Kelainan-Kelainan Sel Darah Sapi Bali (DIFFERENTIAL COUNT AND ABNORNALITIES OF BALI CATTLE BLOOD CELLS) Iwan Harjono Utama; Anak Agung Sagung Kendran; Sri Kayati Widyastuti; Putri Virgania; Sherliyanti Maria Sene; Widarta Dwi Kusuma; Baiq Yunita Arisandi
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Research on blood smear and various disorder in Bali cattle have been conducted at 124 oxen and 76cows. Blood specimens taken from cattle slaughtered cattle in Slaughterhouse Mambal Badung Regency,Bali and blood smear were performed   by a method described elsewhere.Results showed the mean of differential count of lymphocytes, monocytes, neutrophils, eosinophils, basophilsand bands in female Bali cattle bands respectively (average + standard deviation): (47.76 ± 12.13)%, (9.84± 5, 32)%, (39.09 ± 12.55)%, (2.25 ± 2.40)%, (0.33 ± 1.18)%, (0.21 ± 1.01)%. In bali cattle males, thoseresults were : (46.48 ± 8.18)%, (9.84 ± 4.35)%, (40.51 ± 8.29)%, (1.48 ± 1.69)%; (0.45 ± 0.84)%, (0.57 ±0.99)%. Abnormalities that were found are: eosinophenia, monocytosis, Neutrophilia, lymphopenia andlymphocytosis, anisocytosis, poikilocytosis (the presence of akhantocytes, dacrocyt), and hypochromasia.In summary Bali cattle are observed to have abnormalities in the blood cells.
Potensi Air Liur Sebagai Perantara dalam Pemeriksaan Noninvasive pada Hewan Piaraan (POTENTIAL OF SALIVA AS A MEDIATOR FOR THE NONINVASIVE EXAMINATION OF DOMESTIC ANIMALS) Sulaiman Ngongu Depamede; Anwar Rosyidi; Made Sriasih; Dahlanuddin .; Enny Yulianti; Suparman .
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Saliva as unique biological components of the oral cavity has potential as a mediator for noninvasivebiological test. For future livestock industry the application of noninvasive test is strongly necessary dueto animal welfare porpuse. Until now the research and development of the using saliva as a mediator forinvasive test is predominant for humans, while for livestock or domestic animals is still limited. Techniquesand methods that are commonly used are related to proteomics analysis. With this method a lot of thingsthat can be brought about proteomics of saliva are beneficial to the development of saliva-based biomarkers.Although this method is expensive, researches on the use of saliva in the field of livestock industries areurgently needed. It is expected that noninvasive biological test methods based on saliva as a mediator canbe performed immediately.
POTENSI Avicenia marina UNTUK MENGHAMBAT MICROBA DAN MELINDUNGI BENUR UDANG WINDU Gina Saptiani; Andi Noor Asikin; Fikri Ardhani; Esti Handayani Hardi
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (170.175 KB) | DOI: 10.19087/jveteriner.2018.19.1.45

Abstract

The research aims to study potency of Avicennia marina leaf extract to inhibit pathogens in vitro and in vivo on post larvae of tiger prawn. The leaves we re chopped, dried and extracted with water solvents, sea water andethanol. In vitro inhibitory tests were performed using agar disc diffusion (ADD) and minimal inhibitory concentration (MIC) methods. The in vivo test on tiger prawn PL-8 was given by submersion, which then tested with Vibrio harveyi and Saprolegnia sp. The in vitro test showed A. marina extract can inhibit V.harveyi and Saprolegnia sp. In vivo tests of V. harveyi and Saprolegnia sp. infections, showed 60- 88% survival and 57.33-86.67%, with protective ability of the highest pathogen infection of 80.80% and 77.80%. The best A. marina extract that can inhibit microbial and protect the tiger prawn from pathogen infection is ethanol extract with concentration 1,250-1,500 ppm, followed by sea water extract 1,500 ppm and water extract 1,500 ppm respectively
Variasi dan Filogeni Kancil dan Napu (Tragulus Sp.) di Indonesia Menggunakan Gen 12s rRNA Mitokondria (VARIATION AND PHYLOGENI ON GENUS TRAGULUS (TRAGULUS SP.) IN INDONESIA USING MITOCHONDRIAL 12s rRNA GENE) Wirdateti .; Raden Taufiq Purna Nugraha
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Genus Tragulus in Indonesia is consisted of three species, namely Tragulus javanicus, T. napu, and T.kanchil. These three species are distributed in Kalimantan (Borneo), Java, Sumatra and Lesser Sundaislands. Taxonomically, based on morphological characteristic,T. javanicus is the same species as T. kanchil,while T. napu have differences in body size compared to the previous species. The status of genus Tragulusin Indonesia are protected by the law and categorized as least concern for T. napu and data deficient for T.javanicus in the IUCN Redlist. The aims of this study was to look at genetic variation among species andkinship based on mitochondrial DNA gene genus Tragulus. Genes used in this study was 12S ribosomalRNA with 300 bp long, using eight samples orginated from Singkep Islands (four individuals), MountHalimun, West Java (three individuals) and East Kalimantan (one individual). As a comparisons, publishednucleotide sequence of each species from GeneBank database were used. The results showed that thegenetic distances (d) between individuals is high d = 0.322 ± 0.023 with the nucleotide diversity (ð) =0.2402 which indicates that samples was consisted of samples from different species. Phylogenetic treebased on the nucleotide analysis indicatedthat these samples composed of three species namely T. javanicus,T. kanchil and T. napu. Samples from Mt. Halimun and East Kalimantan are clustered in one group,namely T. kanchil (Borneo) and T. javanicus (Mt. Halimun). Two samples from Singkep are clustered indifferent groups with the possibility as T. napu.

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