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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
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KAJIAN FILOGENETIK ULAR SANCA BATIK (Malayopython reticulatus) LOKALITAS JAWA BERDASAR SEKUEN GEN SITOKROM B Slamet Raharjo; Sri Hartati; Soedarmanto Indarjulianto; Rini Widayanti; Alfarisa Nururrozi
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.009 KB) | DOI: 10.19087/jveteriner.2018.19.2.284

Abstract

Reticulated python (Malayopython reticulatus) Javan locality has different phenotype among West Java, Central Java and East Java locality. This research aim was to study the phylogenetic of reticulated python (M. reticulatus) Java locality based on the partial gene cytochrome b sequences. Fifteen males and females adult healthy reticulated pythons (5 from West Java, 5 from Central Java and 5 from East Java) were used in this study. As much as 1 mL blood samples collected from ventral coccygeal vein, put in Eppendorf with EDTA then sent to Laboratory of Biochemistry, Faculty of Veterinary Medicine, Gadjah Mada University for total Deoxyrybo Nucleic Acid (DNA) extraction and DNA amplification with Polymerase Chain Reaction (PCR) method. DNA sequencing was done in First Base Company Singapore by PT. Genetika Science Jakarta. The gene cytochrome b sequences were phylogenetically analysed using Neighbor joining (MEGA v. 5.0) method. The length of partial gene cytochrome b nucleotides sequence of M. reticulatus from West Java, Central Java and East Java locality was 819 bp. The phylogram using Neighbor joining method showed that Malayopython reticulatus Central Java locality was differentiated from West Java locality, while the East Java locality was undifferentiated from West Java and Central Java locality, namely in the same group. It could be concluded that reticulated python (M. reticulatus) Java locality has partial gene cytochrome b sequences with 819 bp. The phylogenetic of Malayopython reticulatus West Java and several from East Java were located at same branch with M. reticulatus GenBank, while the Central Java and several from East Java were located at different branch. It needs further research with more samples and validity of localities for better result.
Identifikasi Staphylococcus sciuri dan S. hominis pada Ikan Kerapu di Pasar Ikan Kedonganan dengan Analisis Sekuen 16S rRNA I Nengah Kerta Besung; Ni Komang Eka Agustiani; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 20 No 3 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (128.811 KB) | DOI: 10.19087/jveteriner.2019.20.3.345

Abstract

Grouper are reef fish which can be cultivated because highly commercial value. There are many different types of bacteria in the digestive tract of grouper, one of them is Staphylococcus. Staphylococcus sp. is a Gram positive, non spora, non motile, and facultative anaerobic. Most of Staphylococcus sp. are pathogenic in human and animals. The purpose of this study was to identify the species Staphylococcus sp. on grouper sold at the Kedonganan fish market, Kuta District, Badung Regency, Bali Province based on 16S rRNA sequence analysis. This study used grouper feces as a samples from 20 fishes. Staphylococcus sp. from feces were cultured in media Blood Agar. Sequence analysis 16S rRNA Staphylococcus sp. was conducted with PCR and sequencing method. This study can identify the bacteria Staphylococcus sciuri and Staphylococcus hominis.
Isolasi, Identifikasi, Sifat Fisik, dan Biologi Virus Tetelo yang Diisolasi dari Kasus di Lapangan (ISOLATION, IDENTIFICATION, PHISICAL, AND BIOLOGICAL CHARACTER OF NEWCASTLE DISEASE VIRUS ISOLATED FROM FIELD CASES) Michael Haryadi Wibowo; Tri Untari; Anastasia Endang Tri Hastuti Wahyuni
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.32 KB)

Abstract

native chicken farm suspected to Newcastle disease (ND) virus infection. Specimens were taken andcollected from the lung was further processed. Suspected materials were inoculated into allantoic sacc inspecific pathogenic free of 10 days embryonating egg chicken. The growth of the virus was determined withthe ability to agglutinate the chicken red blood cells or hemaglutination test. Positive hemaglutinationwas performed with hemaglutinatin inhibition test using specific antibody against ND virus. Method forND virus isolation, propagation and identification were based on the standard procedure of serologicalidentification for ND virus serological identification. 13 out of 34 samples were identified as ND viruses.Observation on the course and time of the virus to kill the chicken embryo could be differentiated intomoderate virus patho-type were 10 isolates and a virulent strains were 3 isolates. Further characterizationbased on the elution time observation indicated 11 isolates were not pathogenic strain and 2 isolates werenot virulent strain. Hemagglutinin stability study revealed that 11 isolates were sensitive being heated at560C for 30 minutes while 2 isolates were resistant. Biological characteristic of ND virus to hemagglutinateon various mammalian red blood cells indicating that most isolates were HA negative. Two isolates wereHA positive with cattle, horse and sheep red blood cell, and one isolate indicated positive HA test by usingsheep red blood cell. Control virus was lentogenic patho-type of La Sota strain showed HA and HI testpositive, elution time was 29 minutes, stability on the hemagglutinin after heating was 2 minutes and HApositive with cattle, horse and sheep red blood cell.
Efficacy of Piperazine Dihydrochlloride Against Toxocara Vitulorum in Buffalo Calves Fadjar Satrija; Yusuf Ridwan; Elok Budi Retnani
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (119.319 KB)

Abstract

A study was conducted to evaluate the efficacy of piperazine dihydrochloride against natural infectionof Toxocara vitulorum in buffalo calves. In the first trial 60 based on fecal ascarid egg count and bodyweight naturally infected calves were, allocated into four groups. Three groups (groups B, C and D) weregiven piperazine dihydrochloride per os at dose levels of 200, 300 and 500 mg per kg body weight,respectivelythe remain group is non-treated controls. Piperazine treatment reduced egg excretion levels at98 to 99 per cent within 7, 14 and 21 days after treatment. Depite no significant differences were foundamong the three doses, highest egg count reductions were observed in calves given the drug 300 mg per kgbodyweight. The second trial were conducted using 10 additional calves. Piperazine significantly reducedT vitulorum egg excretion by 93% within 7 days post the treatment.
Penambahan Glutathione pada Medium Fertilisasi Efektif Mendukung Pembentukan Pronukleus dan Perkembangan Awal Embrio Sapi (SUPPLEMENTATION OF GLUTATHIONE IN FERTILIZATION MEDIUM EFFECTIVELY SUPPORT NORMAL PRONUCLEUS FORMATION AND EARLY BOVINE EMBRYONIC DE Aras Prasetiyo Nugroho; Iman Supriatna; Mohamad Agus Setiadi
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (148.616 KB) | DOI: 10.19087/jveteriner.2017.18.3.327

Abstract

The objective of this study was to determine fertilization rate effectiveness and early embryonic development competency with glutathione (GSH) supplementation in fertilization medium and culture This study consisted of two experiments comprising each of the four treatment and six repetitions with completely randomized design (CRD) using 651 oocytes. In the first experiment, a total of 317 bovine oocytes were matured in tissue culture medium (TCM) 199 at incubator 5% CO2 with temperature 39 ºC for24 h, then fertilized with sperm separated by swim up technique. Oocyte and sperms were incubated in fertilization medium supplemented with 0.25 mM, 0.50 mM, 1.00 mM GSH. In the second experiment, bovine oocytes were matured in maturation medium and fertilized with same procedure as mentioned before, then cultured in modified synthetic oviduct fluid (mSOF) with the following treatment: supplementation GSH only in fertilization medium (T1), supplementation GSH only in culture medium (T2), and supplementation GSH in both fertilization and culture medium (T3), while control not supplementation GSH (T0). Result of the first experiment showed that supplementation 1.00 mM GSH in fertilization medium can increase higher normal pronucleus (2PN) formation (86,9%) compared to other treatments, 0.50 mM (80.3%), 0.25 mM (73.8%), and control (58.9%) (P<0.05). In the second experiment showed that early bovine embryonic development on 2nd day cultured which reached 5-8 cell on treatment T1 (56.0%) and T3 (53.6%) were higher (P<0.05) compared to treatment T2 (26.2%) and T0 (control) (31.3%). Result of the other were showed that early bovine embryonic development on 4th day cultured which reached 9-16 cell on treatment T1 (26.2%) and T3 (27.4%) were higher (P<0.05) compared to that T2 (11.9%) and T0 (control) (10.8%). In conclusion, 1.00 mM GSH supplementation in medium was more effective in supporting normal pronucleus formation and early fertilization bovine embryonic development compared to in culture medium. ABSTRAK Penelitian ini bertujuan untuk mengetahui tingkat fertilisasi dan kompetensi perkembangan awal embrio sapi dengan penambahan glutathione (GSH) pada medium fertilisasi in vitro (IVF) dan kultur in vitro (IVC). Penelitian ini terdiri atas dua penelitian yang terdiri dari masing-masing empat perlakukan dan enam kali ulangan dengan rancangan acak lengkap (RAL) menggunakan 651 oosit. Penelitian I, sebanyak 317 oosit sapi dalam tissue culture medium (TCM) 199 dimatangkan pada inkubator 5% CO2 dan suhu 39°C selama 24 jam, kemudian difertilisasi dengan spermatozoa yang telah diseleksi menggunakan teknik swim up. Oosit dan spermatozoa diinkubasi pada medium fertilisasi dengan penambahan 0,25 mM, 0,50 mM, dan 1,00 mM GSH. Penelitian II, sebanyak 334 oosit sapi dimatangkan pada medium pematangan dan difertilisasi, kemudian dikultur pada medium modified synthetic oviduct fluid (mSOF), dengan perlakuan: penambahan GSH hanya pada medium fertilisasi (T1), penambahan GSH hanya pada medium kultur (T2), dan kombinasi penambahan GSH pada medium fertilisasi dan kultur (T3). Hasil penelitian I, menunjukkan bahwa penambahan 1,00 mM GSH pada medium fertilisasi dapat meningkatkan pembentukan pronukleus normal (2PN) yang lebih tinggi (86,9%) dibandingkan dengan perlakuan yang lain yaitu 0,50 mM (80,3%), 0,25 mM (73,8%), dan kontrol (58,9%) (P<0,05). Penelitian II menujukkan bahwa perkembangan awal embrio sapi pada hari ke-2 kultur yang mencapai pembelahan 5-8 sel pada perlakukan T1 (56,0%) dan T3 (53,6%) lebih tinggi (P<0,05) dibandingkan dengan perlakuan T2 (26,2%) dan T0 (kontrol) (31,3%). Hasil penelitian lain menunjukkan bahwa perkembangan awal embrio sapi pada hari ke-4 kultur yang mencapai pembelahan 9-16 sel pada perlakuan T1 (26,2%) dan T3 (27,4%) lebih tinggi dibandingkan dengan perlakukan T2 (11,9%) dan T0 (kontrol) (10,8%) (P<0,05). Dapat disimpulkan bahwa penambahan 1,00 mM GSH pada medium fertilisasi lebih efektif dalam mendukung pembentukan pronukleus normal dan perkembangan awal embrio sapi dibandingkan pada medium kultur.
LAKTOSA-ASTAXANTHIN MENINGKATKAN KUALITAS SEMEN BEKU KAMBING GEMBRONG DALAM UPAYA KONSERVASI Wayan Bebas; Wayan Gorda; I Gusti Ngurah Bagus Trilaksana; Desak Nyoman Dewi Indira Laksmi; Tjok Gede Oka Pemayun
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (102.052 KB)

Abstract

This research was conducted to produce a formula of diluent for the manufacture of frozen semen of Gembrong goat. Yolk phosphate was used as a basic diluent with the addition of anti-cold shock, antioxidants and combination of anti-cold shock-antioxidant. The research design used a completely randomized design with three kinds of treatments; T1: the addition of lactose 0.6% (anti-cold shock), T2: the addition of astaxanthin 0.004% (antioxidant), and T3: a combination of 0.6% lactose-Astaxanthin 0.004% (combination of anti-cold shock and antioxidants). The addition of DMSO 6% was used in each treatment as intracellular cryo protectants. The freezing process was done with the gradual freezing with conventional techniques. Examination of the quality of semen by thawing prior included progressive motility, viability, and abnormalities. The results showed that anticoldshock-antioxidant combination (0.6% lactose -Astaxanthin 0.004%) produced the best semen quality with progressive motility, viability, and less abnormalities as follow 44.00 + 3.46%, 59.00 + 1.85%, and 14.00 + 0.76% respectively. It was concluded that the addition of a combination of 0.6% lactose-Astaxanthin 0.004% on the phosphate yolk diluent with 6% DMSO as intracellular produced best quality of frozen goat Gembrong semen that suitable for use in artificial insemination and in vitro fertilization.
Gabungan Ekstrak Rimpang Temulawak, Daun Tanjung, dan Daun Belimbing Manis Berdasarkan Electrocardiogram Berpotensi Sebagai Antiaritmia pada Kucing Min Rahminiwati; Widia Safitri; Deni Noviana
Jurnal Veteriner Vol 20 No 3 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (168.377 KB) | DOI: 10.19087/jveteriner.2019.20.3.409

Abstract

Mimusops elengi L. , Averrhoa carambola L. and Curcuma xanthorrihiza Roxb singly were reported to have hypothensif effect. Mechanism underlying decrease of blood pressure was suggested through modification of cardiac activity. The Effect of Mimusops elengi L., combined with Averrhoa carambola L. and Curcuma xanthorrihiza Roxb extract on cardiac activity were studied using ECG on 12 male cats that were grouped to be a control group administered aquadestilata, and treatment group administered extract of 21 mg and 82 mg/2 kg bw orally respectively. The extracts were given 3 h prior to ECG. The results showed a decrease in P wave, QRS complex and the speed of the heart rate after administration of combination of extract . However QT and PR intervals were increased. This showed that the extract can weaken a contraction of the Atria and ventricles, prolonging the onset of the occurrence of atrial contraction towards ventricular contraction, extending the onset of contraction and relaxation of the ventricles and cause a decrease in heart rate. Based the cardiogram, It was concluded that their combination is valuable for treatment of aritmia.
Aktivitas Antifibrotik Ekstrak Buah Delima Terstandar 40% Ellagic Acid pada Tikus Putih (Rattus norvegicus) sebagai Hewan Model (ANTIFIBROTIC ACTIVITY OF POMEGRANATE FRUIT EXTRACT STANDARDIZED 40% ELLAGIC ACID ON RATS (RATTUS NORVEGICUS) AS ANIMAL MODEL) Wiwik Misaco Yuniarti; Bambang Sektiari Lukiswanto
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (118.605 KB)

Abstract

The number of patients with chronic liver disease (fibrosis or cirrhosis of the liver) is increasing fromtime to time. However, until now there is no therapy that really effective to overcome that disease. Therapyfor liver fibrosis typically use substance that have antioxidant activity, anti-inflammatory or anti fibrotic.Various efforts always done to find alternative therapies for liver fibrosis. One of them is the use ofpotential plants that suspected having such activity. Various parts of pomegranate have been shown tohave various activities that beneficial for health. This study was conducted to determine the effect ofpomegranate fruit extract standardized 40% ellagic acid on the improvement degree of liver fibrosis causedby cholestasis by measuring of serum alkaline phosphatase levels (ALP) and gamma-glutamyl transferase(GGT), as a specific indicator of liver damage becaused of cholestasis. The research was conducted by using32 male rats, wistar strain, 2.5 month old, weighing between 150-200 grams. Animal models of liver fibrosis obtained by using BDL technique. Subjects were divided into a control group (P0 = without BDLand giving of pomegranate extract) and treatment groups (P1 = BDL with administration of CMC, P2 =BDL with ellagic acid 90% and P3 = BDL with pomegranate fruit extract standardized 40% ellagic acid).CMC, extract (150 mg / kg BW / PO) and ellagic acid (60 mg / kg BW / PO) administered for 21 consecutivedays in the same volume. At the end of 21 days periods, biochemical evaluation was performed to measureserum levels of GGT and ALP. The result indicated that administration of pomegranate fruit extract ( P3)significantly reduced GGT ( 10.5±9.2 mg/dl) and ALP level ( 509.0±4.2 mg/dl) close to normal level of GGTand ALP ( P0, GGT : 2.8 ± 1.4; ALP : 449.0±62.3) (p<0.05). The level of GGT and ALP in P3 group were lowercompared to the group ellagic acid (P2, GGT=48.5±4.8 and ALP = 691.0± 29.7) and group which only begiven CMC (P1, GGT 191.0±35.4 and ALP 890 ± 5.7) ( p<0.05). Extract of pomegranate fruit thatstandardized with 40% ellagic acid is potential as a antifibrotic agent.
Analisis Kadar Timbal dan Gambaran Darah Gajah Sumatera (Elephas maximus sumatranus) di Pusat Latihan Gajah Sebanga Riau Hamdani Budiman; Al Azhar; Irwandi Yusuf
Jurnal Veteriner Vol 11 No 2 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (118.828 KB)

Abstract

The aim of this research was to determine the lead concentration in blood and hematological profilesof Sumatran elephants. Eight out of 57 captivated Sumatran elephants in Sebanga Elephant TrainingCenter (ETC) Riau were examined for the lead concentration in the blood and for hematological profiles.The results showed that their lead concentration in blood was 0.3425 – 0.4633 ppm with the average of0.3905 ± 0.373 ppm. Their hemoglobin level was 9.60 – 12.10 g/dl with the average of 10.39 ± 1.20 g/dl.Hematocrit levels was 31 – 47% with the average of 37.50 ± 5.21%. In male, their average levels ofhemoglobin and hematocrit were 10.85 ± 1.28 g/dl and 41.00 ± 4.90%, respectively which were higher thanthose in female (9.93 ± 1.06 g/dl and 34.00 ± 2.53%, respectively). Their lead concentration was higher inthe younger elephant than in the older elephants. Although, the lead concentration in blood of Sumatranelephants was high, it appeared to be not related either with their hemoglobin or hematocrit levels. Therewas, however, relationship between the lead level in blood with the age of elephants.
Amplifikasi Gen Penyandi Protein Fusion Virus Tetelo dari Spesimen Lapangan dengan Metode OneStep RT-PCR. (AMPIFICATION OF FUSION PROTEIN ENCODING GENE OF NEWCASTLE DISEASE VIRUS FROM FIELD SPECIMENS BY ONESTEP RT-PCR METHOD) Aris Haryanto; David Kristiawan; Sri Handayani Irianingsih; Dini Wahyu Yudianingtyas
Jurnal Veteriner Vol 14 No 3 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (139.184 KB)

Abstract

Tetelo or Newcastle Disease (ND)  virus is belong to the family Paramyxoviridae, which has a singlestranded RNA (ss RNA) genome and it has viral envelope.  The viral envelope consists of two majorproteins, namely Haemagglutinin/Neuraminidase (H/N) and Fusion (F) protein. Molecular diagnosticmethods OneStep RT-PCR is a commonly diagnostic tool that used to diagnose of Tetelo in poultry. In thisstudy the diagnosis of Tetelo is accomplished by amplification of F protein encoding gene of Tetelo virusdirectly from field specimens without inoculation and propagation of Tetelo virus into embryonated chickeneggs. The objective of this study is to conduct rapid diagnosis of Tetelo virus directly from the field specimensbased on the amplification of the F gene by a OneStep RT-PCR method, so that the results of this study canbe used to assist in the identification of Tetelo virus directly from the field specimens. The results showedthat from the 15 samples of virus which isolated from tracheal swabs of clinical poultry showing symptomsof Tetelo virus infection, a total of 12 samples from 15 tested sampels (80%) are positive tested for Tetelovirus infection. They were indicated by the amplification product of DNA fragments in size of 362 bp.OneStep RT-PCR is a method for rapid and effective diagnosis which can be used  to diagnose of Tetelovirus directly from field specimens.

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