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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
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Akumulasi Timbal dalam Cakar Ayam Kampung Djohan -; Charles Rangga Tabbu
Jurnal Veteriner Vol 11 No 1 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Domestic chickens tend to consume lead (Pb) from their contaminated environment especially in freerangingchickens. Feet of domestic chickens are commonly consumed by many people, making them goodbioindicator to be analyzed for environmental monitoring and food safety purposes. Accumulation of leadin feet and parts of feet (tarsometatarsus bones, digiti, and skin-muscles) were investigated in this study.The average concentrations of lead in whole feet, tarsometatarsal bones, digiti, and skin-muscles were 3.4,3.8, 3.3, and 1.9 ?g.g-1 d.m., respectively. The average amount of Pb accumulated in chicken feet ranges from25.5 to 74.7 ?g. Consumption of 0.5 – 2 chicken feet. A day-1 (low – high intake) results in the exposure of 4,8to 111,5 ?g. individual-1.day-1, which was much higher than the daily exposure standar of 1 ?g. individual-1. day-1. However, very low intake (< 0.1 chicken feet.day-1) results in exposure lower than the recommendedexposure standard. More frequent monitoring and exposure assessment combined with the awareness ofgeneral public on lead contamination in the environment are important for minimizing the risk of leadexposure to human through chicken feet consumption.
Keragaman Gen Heat Shock Protein 70 pada Ayam Kampung, Ayam Arab, dan Ayam Ras (POLYMORPHISMS OF THE HEAT SHOCK PROTEIN 70 GENE IN KAMPONG, ARABIC, AND COMMERCIAL CHICKENS) Mohamad Hasil Tamzil; Ronny Rachman Noor; Wasmen Manalu; Cece Sumantri
Jurnal Veteriner Vol 14 No 3 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of the research was to identify the polymorphisms of HSP-70 gene of kampong, Arabic, andcommercial chickens. The study used whole blood samples taken from the branchial vein at the age of 12weeks from a group of chickens that were kept since DOC. Kampong chickens DOC were obtained byhatching the eggs that were collected from lowlands, medium and high altitude areas in Lombok island,while the Arabic chickens DOC were obtained from farmer. DOC of commercial chickens were purchased atthe poultry shop. The genotyping results using PCR-SSCP analysis showed that kampong and arabicchickens were polimorphic, while commercial chickens were  classified as monomorphic. Kampong chickenshad seven genotypes of HSP-70, namely AA, AB, AC, CC, AD, DD, and BC.  The AD genotypes had thehighest frequency, whereas in arabic chickens had six genotypes, namely AA, AB, AC, CC, AD and BC,which AC genotype had the highest frequencies.  However, the commercial chickens contain only onegenotype which was DD. There were three polymorphic sites found in amplificated area, namely AÆGmutation at site 617, AÆG mutation at site 628, GÆC mutation at site 646,  CÆT mutation at site 661,AÆG mutation at site 699, GÆC mutation at site 754, and AÆG mutation at site 792.  Mutations at sites628, 646 and 661 were considered as silent mutation. HSP-70 gene in kampung and arabic chickenpopulation in this study was in equilibrium. The value of Ho, He, and PIC of kampung and arabic chickenswere not different.
Kejadian dan Terapi Babesiosis dengan Clindamycin pada Kucing (THE INCIDENCE AND TREATMENT OF BABESIOSIS WITH CLINDAMYCIN IN CAT) Retno Wulansari; Raden Roro Soesatyoratih; Suryono .
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The patients : 5 cats, came to “Klinik Hewan Cimanggu”, complains from the clients were includinglistlessness, anorexia, diarrhea, and constipation. From the Physical examination they showed a palemucous membrane, hyperaemic on sclera, larger of cranial abdominal . Laboratory finding on bloodssmear showed blood parasites in red cells that suspected as Babesia sp. One of them concurrently withhaemobartonella sp infections. The general result of blood laboratory test showed anemia andthrombocytopenia. Its treated by clindamycin (10 mg/Kg BW) and multivitamin twice a day for 3 weeksand parasitemia level in 1000 red cell was count before treated. Reexamination of smear red cell wasdone during and after treatment. In general they had demonstrated the decrease of parasitemia level.Some of them didn’t showed any changes of parasitemia level, however they showed morphological changesthat indicate inactive condition of parasites. The decrease of parasitemia level or the morphologicalchanges of parasites indicates that the development of parasitemia level has been depressed, so theclinical signs decreased and the animal’s condition improved. It believed that clindamycin inhibits proteinsynthesization in ribosome causing the damage to the parasite, but it will not eliminate the parasitesrapidly from peripheral blood. The Clindamycin treatment on cats with babesiosis will not induct the sideeffects .
Studi Epidemiologi Agen Zoonosis Escherichia coli O157:H7 melalui Analisis Random Amplification of Polymorphic DNA (RAPD) I Wayan Suardana; Wayan Tunas Artama; Widya Asmara; Budi Setiadi Daryono
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Epidemiological studies of zoonotic agent Escherichia coli O157:H7 have been analyzed pheneticallyand or phylogenetically. In a phenetic classification, micoorganisms are arranged into groups (phena) onthe basis of high overall similarity using both phenotypic and genotypic methods without judgementaspect of its ancestry or evolutionary. Due to its importance to epidemiological aspect, the study of geneticvariation of isolates origin from some sources need to be conducted in order to trace the routes of infection.A total of 20 samples obtained from some sources i.e clinically human feces, non-clinically human feces,cattle feces, chicken feces, and beef feces were used in this study. The study was started by confirming allof the isolates using O157 latex agglutination test and H7 antiserum test, followed by genomic DNAanalysis by random amplification of polymorphic DNA /RAPD methods. RAPD results were analyzed using a simple matching coeficient (Ssm) and alogorhythm unweighted pair group method using arithmeticaverages (UPGMA) programe. Results showed there were range of genetic DNA from local isolates (75.1–99,6%) which was almost similar to ATCC 43894 control isolate. The highest similarity (99.6%) to ATCC43894 control was showed by SM-7(1) isolate obtained from cattle fecal and KL-68(1), isolate obtainedfrom clinically human fecal. In addition, KL-52(7) obtained from clinically human fecal had high similarity(99.6%) to MK-35 isolate obtained from chicken fecal. On the other hand, DS-21(4) and DS-16(2) isolatesthat were obtained from beef had high similarity (84.9%) to other isolates including ATCC 43894 controlisolate. The highest similarity of E. coli O157:H7 isolates that were obtained from cattle feces, beef, andchicken feces to human feces isolate indicated that there were both cattle and chicken were potentialreservoirs of the zoonotic agen which can be transmitted to human.
Perbandingan Sekuens Konsensus Gen Hemaglutinin Virus Avian Influenza Subtipe H5N1 Asal Unggas di Indonesia dengan Subtipe H5N2 dan H5N9 I Gusti Ngurah Kade Mahardika; I Nyoman Suartha; Ida Bagus Kade Suardana; I Gusti Ayu Yuniati Kencana; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 10 No 1 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Consensus sequence of hemagglutinin (HA) gene of avian influenza viruses of H5N1 subtype isolatedfrom fowl in Indonesia – hereafter named as H5N1_Indonesia – is compared with that of H5N2 and H5N9viruses. Sequence information were downloaded from the public database GeneBank. The genetic distancesand nucleotide as well as its deduced amino-acid sequence alignment were analysed. At nucleotide level,genetic distances of HA between H5N1_Indonesia to H5N2 and H5N9 are 16.2% and 9.6%, respectively.At amino-acid level, the distances are 9.7% and 6.8%. The genetic distances of HA1 fragments are 19.0%(H5N1_Indonesia – H5N2) and 10.9% (H5N1_Indonesia – H5N9). At amino-acid, level the genetic distancesof HA1 are 13.1% (H5N1_Indonesia-H5N2) and 8.8% (H5N1_Indonesia – H5N9). All three subtypes havedifferent glycosilation motive and variation of amino-acid sequence of four antigenic sites. The consequenceof those facts is discussed.
Identifikasi Koi Herpesvirus dengan Uji Imunopatologi Imunohistokimia Streptavidin Biotin pada Ikan Mas Karier (IDENTIFICATION OF KOI HERPESVIRUS USING IMMUNOPATHOLOGIC IMMUNOHISTOCHEMISTRY OF STREPTAVIDIN BIOTIN IN THE COMMON CARP CARRIERS) Raden Wasito; Hastari Wuryastuti; Bambang Sutrisno
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

In  managing the koi herpesvirus (KHV) outbreaks as a routine national program in Indonesia, testingbased on biotechnology, such as  immunopathologic immunohistochemical approach(es) using antibodythat is safe, rapid  and accurate need to be applied. This will hopely assist the Government of Indonesianin improving and enhancing the sustainability of national animal proteins program. The present studywas aimed to develop and apply the immunopathologic immunohistochemistry of streptavidin biotin (IHCSB) for detection of KHV in the apparently normal carps. The gills from 48 common carps  (Cyprinuscarpio) that appear to be healthy were prepared for  DNA-based KHV  by IHC SB.  Common carps werecollected from fish farms which had an outbreak of KHV in 2008-2009 in Yogyakarta.  All fish werenecropsied. The gills were processed histopathologically and then stained for IHC SB with monoclonalantibody anti-KHV. We demonstrated that all of the fish gills were positive for KHV antigen. Thus, it isconcluded that method is useful and consistent, very sensitive and rapid, and is a reliable method to beapplied for field condition to detect antigen KHV in the gills of normal, healthy looking carps.   In addition,and more importantly, the fish can act as a source of KHV (carriers for KHV) and may result in the spreadof diseases among susceptible fish.
Sidik Jari DNA dan Fenotipe pada Populasi Kambing Gembrong dengan Status Kritis di Karangasem, Bali (DNA FINGERPRINT AND PHENOTYPE ON THE CRITICAL POPULATION STATUS OF GEMBRONG GOAT IN KARANGASEM REGENCY, BALI) Moch Syamsul Arifin Zein; Sri Sulandari
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Gembrong goat is animal genetic resources which endemic in Bali island and only exists in Karangasemregency. The current population status of the gembrong goat is critical. Therefore, the population developmentof in-situ or ex-situ needs to be conducted. Close relative mating tend to occur in conditions such as in asmall population, critical status or limited distribution. Low degree of genetic variability is often found insuch conditions. Mating arrangements based on DNA data of fingerprint was developed in this study toavoid mating between close relatives (inbreeding). Pedigree detection was analyzed using fifteen (15)microsatellite markers as recommended by ISAP/FAO. The results showed that polymorphic alleles foundin the microsatellite markers of SRCRSP3, ILSTS005, MCM527 (4 alleles), followed BM1818 (3 alleles),and ILSTS029, BMS1494, MAF035, OARFCB20, OARE54, MAF70, ILSTS11, ETH10 (2 alleles).Monomorphic allele (1 allele) was also found in microsatellite markers of SPS113, CSRD247, INRA0132.DNA fingerprint of the gembrong goat population was created by genetic distance between individuals,and indicated six clades / haplogroups. It is suggested from this study that mating arrangements betweendifferent clades should be applied to increase genetic diversity. Description of morphologies such as haircolor and body size, were used as basic considerations in determining the authenticity of gembrong goat.Among the population of gembrong goat in this study, hair color of the goat showed white (78.95%), amixture of light brown and white (15.79%), and a mixture of brown and black (5.26%). Gembrong goat bodysize in this study was still in the range of the previous studies. The analysis results of DNA fingerprintsand phenotypes can be used as a basis for rescue and development of gembrong goats, in an attempt toform a large gene pool with high viability for the conservation, development and sustainable uses.
Penanganan Obstruksi Usus pada Anjing: Laporan Kasus Erwin Erwin; Rusli Rusli; Amiruddin Amiruddin; Deni Noviana; Raden Roro Soesatyoratih; Arni Diana Fitri; Sitaria Fransiska Siallagan
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.77 KB) | DOI: 10.19087/jveteriner.2018.19.1.137

Abstract

Veterinary Hospital of Education Faculty of Veterinary Medicine, Bogor Agricultural University, received a Golden Retriever with clinical symptoms of anorexia, abdominal pain, vomiting and constipation in April 2016. Blood profile examination showed leukocytosis, erythropenia and low hemoglobin level. Radiographic examination without contrast showed a foreign body which is characterized by a large mass radiopaque in intestinal area. Forty-five minutes after the administration of radiographic contrast, contrast material was still in gastrium and only reached partial intestinal. Endoscopy examination showed there was irritation symptoms of the esophagus to gastrium. Black colored liquid was seen while the endoscope inserted into the gastric. Enterotomy was carried out to remove foreign objects. The foreign body is consisted of bones fragments and the plastic that was eaten by the patient. One week after surgery, the animals showed clinical symptoms and had a good appetite. These case can be prevented by not giving foods that contain animal bones and keeping animals in a dirty environment.
The Effectiveness of Red Yeast Rice Antioxidant on Performance of Japanese Quail (Coturnix coturnix japonica) Induced Oxidative Stress through Dexamethasone Administration Nuri Ardiani; Koekoeh Santoso; Hera Maheshwari
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (90.411 KB) | DOI: 10.19087/jveteriner.2019.20.2.219

Abstract

Red yeast rice is a fermented product of rice and mold Monascus purpureus. Red yeast rice contains secondary metabolites such as lovastatin, phenol and flavonoids that have potential as antioxidants. This study was aimed to examine the effectiveness of red yeast rice antioxidants on performance of japanese quail administered with dexamethasone through the parameters of feed digestibility, feed consumption, growth in body weight, stress index, malondialdehyde (MDA) andsuperoxide dismutase (SOD). This study used a factorial completely randomized design. The animals used in this study were 60 quails (Coturnix coturnix japonica), with a body weight of 140195 grams. Group K0 was a control, group D were quails given only dexamethasone at a dose of 1.25 mg/g BW for 7 days after one-week adaptation. Group D+VC were quails given dexamethasone at a dose of 1.25 mg/g BW then added with vitamin C at a dose of 20 mg. Group D+K1, D+K2, and D+K3 were quails given dexamethasone then orally given red yeast rice of 18 mg, 24 mg, and 30 mg for 14 days. The feed consumption, feed digestibility and body weight shows no significant difference among group (P>0.05). The heterophyl/lympocyte ratio was elevated when dexametasone administered and tended to decrease when administered with red yeast rice. Result also showed that red yeast rice has the potential as an antioxidant in quail administered with dexamethasone as seen from the tendency of decreased level of MDA and increased level of SOD enzyme. The results also showed that quail administered with dexamethasone decreased level of MDA and elevated levels of SOD enzyme in the liver after administration of red yeast rice.
Pelacakan Ekspresi Antigen Toxoplasma gondii secara Imuno(sito)histokimia (TRACKING EXPRESSION OF TOXOPLASMA GONDII ANTIGENS USING IMMUNO(CYTO)HISTOCHEMISTRY METHOD) Ida Ayu Pasti Apsari; Ida Bagus Oka Winaya; Tjokorda Sari Nindhia; Ida Bagus Ngurah Swacita
Jurnal Veteriner Vol 18 No 4 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.568 KB) | DOI: 10.19087/jveteriner.2017.18.4.535

Abstract

The research objective want to track the expression of Toxoplasma gondii antigenes in the heart of the free-range chicken with immuno(cyto)histochemistry technique. In the present study were examined three methods to track antigen T.gondii, first method direct microscopic examination of the heart digests, the second method to detect antigen of T.gondii with immunocytochemistry technique of the chicken heart digests and the third immunohistochemistry examination of the heart free-range chicken. The number of material samples examined were 100 heart free-range chicken. Direct microscopic examination of the heart digests free-range chicken to track the bradyzoite form (inside cyst). Examination by immuno(cyto)histochemistry technique keep track T.gondii an antigen expression on cells and heart muscle tissue. The results showed that the direct microscopic examination on the heart tissue unable to detect cyst and antigen T.gondii. Immunohistochemical examination successfully detected the expression of antigenes T.gondii and was found 2% (2/100) positive. It can be concluded that T.gondii antigen expression in the heart of range chicken could be detected by immunohistochemistry.

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