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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
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Real Time Polymerase Chain Reaction : Perangkat Diagnostic Alternatif untuk Melacak Virus Nipah (REAL TIME POLYMERASE CHAIN REACTION : AN ALTERNATIVE DIAGNOSTIC TOOL TO DETECT NIPAH VIRUS) Indrawati Sendow; Atik Ratnawati; Raden Mas Abdul Adjid; Muharam Saepulloh
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Nipah is a dangerous zoonotic disease with a high social, economical and psychological impact. Fruitbat Pteropus sp. is one of the nipah virus  reservoir host. As the virus is categorized as a dangerous zoonoticdisease that cause fatal in human, all works related to live virus should be conducted in a laboratory withBSL4 facilities. The detection of nipah virus using real time PCR to replace virus isolastion can thereforebe conducted in a laboratory without BSL4 facilities. The results was further  confirmed at referencelaboratory at   Australian Animal Health Laboratory ( AAHL) Geelong, Australia, indicated that nipahvirus can be detected in saliva of fruit bat P. vampyrus in Medan North Sumatera.
Antigenisitas, Sensitivitas, dan Spesifisitas Protein Toxocara canis pada Pemeriksaan Antibodi Serum Mencit dengan Indirect-ELISA Sri Subekti Bendryman; Kusnoto Kusnoto; Tutik Juniastuti
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this research were to determine antigenicity, sensitivity, and specificity of Toxocara canisprotein used as antigen in indirect-ELISA for the detection antibody against the worm in the infected hostin order to proper diagnose kit. The design used was true experimental, with Post-test Only ControlGroups Design. Mouse was immunized with various worm homogenates used to antigenicity, sensitivityand specificity tests of T. canis protein with indirect-ELISA technique. The independence variable werevarious immunogens (homogenates); the dependence variables were antigenicity, sensitivity and specificityvalues interpreted by optical density (OD) value of mouse sera; and controlled variable were mouse strain,feed and retrieval time of sera. The result showed that OD values of mouse sera immunized with T. canisand T.cati homogenate were signicantly difference (p<0.01) as compared to those immunized withAncylostoma spp., Dipylidium caninum and control sera. Using the diagnosis based on the finding ofToxocara, the sensitivity of OD value by ELISA result from mouse sera immunized with Toxocara spp.homogenate were 100%. Using negative OD value by ELISA from mouse sera immunized with Ancylostomaspp. and D. caninum homogenate, the specificity of the test was 87.5%. In conclusion, protein of T.canishas the same antigenicity against anti-T. canis and anti-T. cati sera, but they had the lower antigenicityagainst anti-Ancylostoma spp. and anti-D.caninum sera. As the sensitivity value of 100% and specificityvalue of 87.5%, in detecting antibody against toxocariasis, the possibility of obtaining false positive was12.5%.
Kualitas Tulang Tikus Betina Normal yang Diberi Ekstrak Sipatah-patah pada Masa Pertumbuhan Sabri Mustafa; Nurhidayat -; Koeswinarning Sigit; Bambang Pontjo Priosoeryanto; Wasmen Manalu
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The experiment was designed to study the effects of sipatah-patah extract (ESP) on bone growth ingrowing female rats in order to prevent osteoporosis during postmenopausal period. Twenty growing femaleSprague Dawley rats in similar body weight were used with the average of age was 20 days old. Theexperimental rats were randomly divided into 5 (five) groups: control group (NOV-0, administered carboxylmethyl cellulose 1%) and groups administered ESP at the age of 30 days (NOV-1), 60 days (NOV-2), 90days (NOV-3), and 120 days (NOV-4) with dosage of 750 mg/kg body weight daily. Blood samples werecollected every 30 days to analyze serum calcium and phosphate concentrations. At the end of the treatment(180 day old), all of the animals were sacrificed to measure and analyze bone growth histologically. Ostibia-fibula slice was stained by using Hematoxylin Eosin (HE) method to observe osteoblast and osteoclastdensities, while Masson trichrome was used to observe trabeculae structure. The results showedadministration of ESP in rat during growing period improved bone growth. NOV-1 with longer duration(150 days) showed better growth rate with longer femur, optimum serum calcium, and phosphateconcentrations, and higher number of osteoblast with lower osteoclast densities compared to control rats(NOV-0). It was concluded that giving ESP at earlier age with longer duration during growth period couldimprove bone growth. This condition was expected could also improve the bone conditions during growingperiod.
Diet Fosfor Tinggi Penyebab Osteodistrofia Fibrosa pada Tikus (HIGH PHOSPHOROUS DIET CAUSED OSTEODISTROFIA FIBROSA IN RATS) Hartiningsih .; Raden Wasito
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this research was to study the impact of high phosphor diet on the femur of ratsconsuming soybean. Ten female rats at 4 weeks of age were randomly divided into two groups of five, NP(rats fed with normal phosphor diet) and HP (rat fed with high phosphor diet). Each rats was placed intoindividual cages at 22-25°C. All  rats were given normal diet and water which were  provided ad libitum.The rats were also adaptation for three weeks before the treatment was given.  At seven weeks of age, ratsin NP group were fed normal diet (calcium:phosphor=0,6%:0,4%), while rats in HP group were fed highphosphorus diet (calcium:phosphor=0,6%:3,6%) for 6 weeks. At the end of the study, blood was collectedfrom plexus retroorbitalis for calcium and phosphor analysis, while right femur was taken forhistopathological examination using hematoxylin and eosin stain. The research results showed that bloodof calcium was significantly reduced (P<0.01) in HP group compared with NP group, while blood phosphorwas significantly increased (P<0.01) in HP group. Histopathological analysis of the proximalis femur inNP group showed the osteogenic zone of physis and the trabecular bone speculum of metaphysis werenormal, while the osteogenic zone of physis and the trabecular bone speculum of metaphysis in HP groupwere irregular.  Fibroblast in  trabecular bone speculum of bone marrow were also observed.  It can beconcluded that high phosphorus diet may cause osteodystrophia fibrosa in rats.
Praktek Sanitasi Higiene pada Usaha Pengolahan Dangke Susu Sapi di Kabupaten Enrekang, Sulawesi Selatan (THE PRACTICES OF HYGIENIC SANITATION IN PROCESSING INDUSTRIES OF COW MILK DANGKE IN ENREKANG DISTRICT, SOUTH SULAWESI) Wahniyathi Hatta; Mirnawati Bachrum Sudarwanto; Idwan Sudirman; Ratmawati Malaka
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Lack of hygienic sanitation conditions in food processing industry could contribute to the food safety.The aim of this study was to assess the implementation of hygienic sanitation practices in the processingindustries of cow milk dangke in Enrekang District, South Sulawesi. Total of 60 respondents wereinterviewed using questionnaire and observation on hygienic sanitation practices was carried out usingcheck-list.  Respondents were producers and also workers in the processing units in Cendana Sub-district,Enrekang which had been selected with simple random sampling technique.  Scoring of hygienic sanitationcondition were based on the application of hygienic sanitation practices on the checklist.  None of workerswore special clothes, put dangke molds in closed container, and wiped packaging of dangke with specialclothes.  Less than 50% of the workers  wore a head coverings, prepared papaya latex with boiled water,and covered dangke when molding.  A total of 50% of the workers immediately washed the molds afterused.  More than 50% of the workers washed their hands with soap before dangke processing, washed themolds with soap, wiped the packagings with a cloth before it used, stored the milk in a closed container,and cleaned papaya fruit before preparing papaya latex.  All of the workers made dangke while doinghousework. Hygienic sanitation condition of dangke processing industries that had good category was 3%while another 57% in moderate category and poor category was 40%.  The hygienic sanitation practices hadnot been fully implemented on the processing industries of cow milk dangke in Enrekang district, which ledto the category of hygienic sanitation condition of largely of them was moderate .
Pengimbuhan Ragi Roti dalam Pakan Meningkatkan Respons Imun Nonspesifik dan Pertumbuhan Ikan Nila (SUPPLEMENTATION OF BAKER’S YEAST IN FEED ENHANCE NONSPECIFIC IMMUNE RESPONSE AND GROWTH OF NILE TILAPIA) Henky Manoppo; Magdalena EF Kolopita
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study was carried out to evaluate the efficacy of Baker’s yeast (Saccharomyces cereviciae) to enhancenonspecific immune response and growth of Nile tilapia fish (Oreochromis niloticus). The fish were obtainedfrom Freshwater Hatchery Tateli (BP3I), Marine and Fisheries Office, North Sulawesi. After acclimatizationfor two weeks in 1000 L fiberglass tank, fish with an average weight of 9 g were put in five 45 L-aquaria ata density of 15 fish/aquarium. During the experiment, fish were fed with feed pellet supplemented withfive different doses of baker’s yeast (10, 20, 30, 40 g yeast/kg pellet) for four consecutive weeks at 5% bw/day, twice a day. Fish in different aquarium received different dose of baker’s yeast. Immune parametersincluding total leucocyte count and phagocytosis activity and growth of fish were measured at two weeksinterval. After four weeks of feeding, total leucocyte count and phagocytosis activity of phagocyte cells offish fed pellet supplemented with 10 g yeast/kg pellet increased significantly as compared to that ofcontrol fish (p<0.01). Growth of fish fed pellet supplemented with 10 g yeast/kg pellet also increasesignificantly as compared to control group (p=0.01). Average weight gain of fish fed pellet supplementedwith 10 g yeast/kg pellet was 15.00±1.00 g while control fish was 8.33 g. As conclusion, supplementationof baker’s yeast in feed could enhance nonspecific immune response and growth of Nile tilapia fish.
Cemaran Escherichia coli pada Tepung Telur yang Diimpor Melalui Pelabuhan Tanjung Priok, dan Resistensinya Terhadap Antibiotik (ESCHERICHIA COLI CONTAMINATION ON EGG POWDER IMPORTED THROUGH PORT OF TANJUNG PRIOK AND ITS RESISTANCE AGAINST ANTIBIOTICS) Kamil Riski Sidik; Denny Widaya Lukman; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Eschericia coli contamination was found on egg powder imported to Indonesia during microbialexamination. The study was conducted to describe the presence of E. coli and its resistance to antibioticsin egg powder imported to Indonesia through The port of Tanjung Priok. Samples was determined usingcross sectional study and the sample size was calculated based on assumption of confidence level of 95%with margin of error of 10% and predicted prevalence of 50%. Total of 100 egg powder samples wascollected in August 2014 from two exporting countries, Ukraine (whole egg powder, n=30) and India (wholeegg powder, n=40 and egg yolk powder, n=30). Examination was performed by packaging and label inspectionof the product followed by samples collection and testing for bacterial contamination using rapid test andconventional isolation and identification methods. Detected E. coli was isolated and then tested forantibiotic resistance. Examination results showed that 4 samples were positive to E. coli. E. coli isolatesshowed resistance against 6 types of antibiotics and 75% of the isolates had resistance against minimumof 3 types of antibiotics. These conditions should be taken into consideration since antibiotic resistance inE. coli would cause negative impacts on human, animal and environmental health.
Keterkaitan antara Turbiditas Serum dan Laju Endap Darah dengan Kerusakan Hati pada Sapi Bali Iwan Harjono Utama; Yanne Yanse Rumlaklak; Dewa Ayu Dwita Karmi; Anak Agung Sagung Kendran; Sri Kayati Widyastuti; I Ketut Berata; Luh Eka Setiasih
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research was aimed to observe serum turbidity and blood sedimentation rate (ESR) as a predictorof hepatic damage in bali cattle. Two hundred whole blood and sera from 80 male and 120 female bullswere sampled from Mambal abbatoir, Badung, Bali. All blood were examined for their ESR and sera werefor their turbidity using ZnSO4 solution, besides we observed some hepatic damages pathology anatomicallywithout incision such as : internal bleeding, formation of connective tissue, swelling, and bile-ductenlargement. all of those damages were scaled graded from 0 (without abnormality) to 4 (more than 75%liver surface has abnormalities). Results showed all 86% bulls (male 32% and female 54%) have their seraturbidity ranged from 1,01-2,00, besides, 89% bulls (45% male and 44% female) have their ESR rangedfrom 3-8 mm in 24 hours. Most of liver abnormalities were : swelling (58%), bile-duct enlargement (50,5%),connective tissue formation (73%), and bleeding (59%) most of them were falling in scale 1 (less than 25%of liver surface area). Also, ESR has positive correlation (P<0,05) with connective tissue formation andserum turbidity value could be used to predict internal bleeding and connective tissue formation. It couldbe concluded ESR value could be used as a predictor of connective tissue formation and serum turbidityvalue could be used as a predictor for internal bleeding and connective tissue formation in bali cattle.
Bioaktivitas Forbazol-E terhadap Kerusakan Ultrastruktur Dinding Sel Staphylococcus aureus Ni Putu Ristiati; Ketut Suata; Dewa Ngurah Suprapta
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The bioactivity role of phorbazol-E in inhibiting bacterial protein synthesis is unknown. Onthe contrary, phorbazol-A, B, C, and D have been proved to inhibit the bacterial protein synthesis.The effect of forbazol-E on Staphylococcus aureus ATCC25922 cell wall ultrasctucture destructionwas observed. The study used randomized-post test-only control group design which consist of threetreatment (control, treatment I and II) with 9 repeatation. Treatment I: 1 ml overnight culture ofS. aureus was cultured into a mixed of 20 ml Mueller Hinton (MH) broth and 37.5 mg/L phorbazol-E; whereas in treatment II phorbazol-E used was 75.0mg/L. The growth curve of S. aureus wasmonitored using spectrophotometer; whilst the destruction of cell wall ultrastructure was observedusing Transmission Electron Microscope (TEM) at Eijkman Insitute, Jakarta. Phorbazol-E at75.0mg/L significantly caused the destruction of the bacteria cell wall ultrastructure and inhibitedthe bacteria growth in comparison to phorbazol-E at 37.5 mg/L (p<0.05).
PREGNANT MARES SERUM GONADOTROPHIN INCREASES NUMBER OF EGGS AND ACCELERATES LAYING PERIOD OF BALI DUCK WITH DELAYED EGG PRODUCTION Made Kota Budiasa; Wayan Bebas
Jurnal Veteriner Vol 9 No 1 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study was carried out to determine the effect of Pregnant Mares Serum Gonadotrophin (PMSG) on the weigth of ovary, the number of developing follicles and the onset egg production in ducks with delayed egg production. In this study, as many as 40 Bali ducks were used and they were divided into 4 groups in a completelty randomized design i.e. control group (TO) treated with NaCl 0,9%, group T1 treated with PMSG dose 5 IU per duck, group T2 treated with PMSG dose 10 IU per ducks and group T3 treated with PMSG dose 20 IU per duck intramuscularly. The weight of ovary increased significantly with the increasing dose of PMSG, i.e dose 0 IU (21,04 + 3,90 gram), dose 5 IU (25,40 + 5,31 gram), dose 10 IU (49,52 + 5,05 gram) and dose 20 IU (59,30 + 4,66 gram). The number of developing folilicles also increased significantly with the increasing dose of PMSG, i.e dose 0 IU (1,4 + 0,5 follicles), dose 5 IU (2,4 + 1,34 follicles), dose 10 IU (5,8+2,07 follicles) and dose 20 IU (8,6+1,67 follicles). In addition, the onset of egg production was shortened significantly with the increasing dose of PMSG, i.e. dose 0 IU (19,0+1,58 days), dose 5 IU (16,8+1,3 days), dose 10 IU (14,2+1,92 days) and dose 20 IU (9,6+14 days). PMSG treatment was proven to increase the egg production capability of ducks with delayed egg production.

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