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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
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Peningkatan Kadar Asam Lemak Tidak Jenuh Susu Sapi Friesian Holstein melalui Suplementasi Minyak Jagung Terproteksi dengan Berbagai Level Protein Ransum Danes Suhendra; Sudjatmogo Sudjatmogo; Widiyanto Widiyanto
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (108.333 KB) | DOI: 10.19087/jveteriner.2018.19.1.100

Abstract

This study was aimed to examine level supplemenation of corn oil (CO) as a source of protected poly unsaturated fatty acids (PUFA) and various crude protein (CP) levels in diets to ruminal iodin number and milk fatty acids of Friesian Holstein. The research done through two stages, using in vivo method and in vivo method. The corn oil protection is performed by saponification using KOH and then tranformed using CaCl2 to calcium salt. Research use two treatment factors with three replications, the first factor was supplementation of PUFA (L) with details L0 (Without protection), L1 (supplementation 75% Protected CO), and L2 (Supplementation 80% Protected CO) and the second factor is the P1 crude protein level (CP 12%) and P2 (CP 16%). The results showed that there was no interaction effect between the supplementation of protected CO with protein level to the ruminal iodin number, saturated fatty acid (SFA), unsaturated fatty acid (UFA), linoleic acid (LA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acid (PUFA) milk. The parameters are iodin number and milk fatty acids. Result of this research show that supplementation of protected CO increased the ruminal iodin number (P<0.01), UFA (P=), LA (P=) and milk PUFA (P=). Supplementation protected CO decrease milk SFA (P=). It can be concluded that supplementation of protected CO increases milk UFA of FH.
Molecular Detection of Toxoplasmosis Using Specific Primers P30, B1, and rDNA Wisnu Nurcahyo; Joko Prastowo; Ana Sahara
Jurnal Veteriner Vol 13 No 1 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (137.706 KB)

Abstract

Study in order to develop molecular techniques using specific primers for the early diagnosis oftoxoplasmosis have been conducted. Detection of Toxoplasma gondii genome was performed usingpolymerase chain reaction (PCR) technique. The primers used in this study were rDNA, P30, and B1. ThePCR products were further run using gel electrophoresis (gel 1.5% – 2.0%) and the band was documented.Toxoplasma was detected at 500 bp and 600 bp using primer P30 and B1, respectively. Whereas usingprimer rDNA no band was observed. It was assumed that primer rDNA was not sensitive since the targetamplification was 88 bp.
Kompetensi Perkembangan Oosit Kambing Kacang dengan Diameter Berbeda pada Medium yang Disuplementasi Cairan Folikel (DEVELOPMENTAL COMPETENCE OF KACANG GOAT OOCYTES WITH DIFFERENT DIAMETER ON MEDIUM WITH FOLLICULAR FLUID SUPPLEMENTATION) Ali Harris; Sri Rahayu; Gatot Ciptadi
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (86.052 KB)

Abstract

The purpose of this study was to determine the developmental competence of local goats oocytes on thebasis of their diameter in the medium supplemented with follicular fluid. Ovaries obtained fromSlaughterhouse, Malang City were collected and washed with NaCl containing streptomycin and penicillinat 32-35oC. Oocytes were obtained by follicular aspiration using a 10 ml syringe and needle 18 G needle.Oocytes were then divided into 3 groups on based at their diameter, group 1 (<160,5 ?m), group 2 (160,5-170,0?m) and group 3 (> 170,0?m). Follicular fluid was obtained by pooling the goat follicular fluid fromfollicles which have 3-8 mm in diameter. Maturation medium used was TCM-199 + 10% follicularfluid.Oocytes matured for 26 hours at 39oC and 5% CO2 with maximum humidity. Observation of oocytedevelopmental competence includes level cumulus cell expansion and maturation level of the nucleus. Theresults showed that the oocytes in groups 2 (160,5-170,0?m) and 3 (> 170,0?m) have a maximum ofcumulus expansion level 1 (63,8 and 74%) and metaphase II (56,6% and 68,5%).It is concluded that thediameter of oocytes had an influence on oocyte developmental competence (p <0,05), and can be consideredfor oocytes selection criteria.
Faktor-Faktor yang Mendorong Kejadian Rabies pada Anjing di Desa-Desa di Bali (FACTORS ENCOURAGING THE INCIDENCE OF RABIES IN DOGS IN VILLAGES IN BALI) I Wayan Batan; I Ketut Suatha
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (84.512 KB)

Abstract

Rabies has been spread to all over part of Bali, caused 160 people and thousands of dogs’ death. Theeffort for preventing rabies have been done, many villages after six months of eradicating rabies programmedhave been free, but a few villages still infected with the rabies. The aim of the study was to find the factorsthat make a number of villages are still infected with rabies. The study was conducted by doing a fieldsurvey to the nine districts of Bali. The survey was aimed at villages that remain infected despite effortsto control rabies has been done in the village. The device of survey is equipped with open ended and closedended questionnaire to be asked to the respondents. Characteristic villages infected with rabies will befigured out after the data were analyzed descriptively. The risk factors that prominent that make Baliinfected rabies include: dogs kept by releasing freely; presence of rabies carrier animals other than dogs;dog free contact with other dogs; the dogs were kept without having fed; puppies obtained from others; theflow of the dogs in and out to the village; And many communities have not obtained adequate counselingregarding the rabies. The conclusion that may be drawn is that there are some of factors that make Baliremain infected rabies.
Cover, Redaksi, Daftar Isi JVet 17 (3) Jurnal Veteriner
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (499.455 KB)

Abstract

Cover, Redaksi, Daftar Isi JVet 17 (3)
Prevalence of Intestinal Worm in Free Ranging Domestic Cats in Bali (PREVALENSI CACING USUS PADA KUCING PELIHARAAN YANG BEBAS BERKELIARAN DI BALI) I Made Subrata; Ida Bagus Made Oka; Kadek Karang Agustina
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (87.504 KB) | DOI: 10.19087/jveteriner.2017.18.3.441

Abstract

The aims of this study were to identify and to measure the prevalence of intestinal worm infections in free-ranging domestic cats in Bali. As many as 133 cat fecal samples were collected from Bali and preserved in sodium acetic formaldehyde solution. Coproscopy method (sedimentation concentration and flotation techniques) was used to identify the eggs of helminthes. Based on fecal examination, eggs of four helminthes species : Toxocara, Ancylostoma, Cestoda and Capillaria were identified. This result indicates the prevalence of intestinal worms in free ranging domestic cats were high, consisting of Toxocara sp (71.43%), Ancylostoma sp (37.59%), Cestoda (19.55%) and Capillaria sp (0.75%). Therefore, it is needed to conduct programs to reduce and eradicate that helminthes. ABSTRAK Penelitian ini bertujuan untuk mengidentifikasi jenis cacing dan mengukur prevalensi infeksi kecacingan pada kucing yang diliarkan di Bali. Sebanyak 133 sampel feses kucing yang berasal dari seluruh Bali dikumpulkan dan disimpan dalam larutan sodium acetic formaldehide. Seluruh sampel diperiksa secara koproskopi dengan dua metode berbeda yaitu metode konsentrasi sedimentasi dan metode pengapungan untuk mengidentifikasi telur cacing yang terdapat pada feses kucing. Pada penelitian ini teridentifikasi empat jenis cacing yang menginfeksi kucing yang diliarkan di Bali yaitu Toxocara, Ancylostoma, Cestoda dan Capillaria. Hasil penelitian ini menunjukkan bahwa prevealensi infeksi kecacingan pada kucing yang diliarkan di Bali masih tinggi, yaitu Toxocara sp (71,43%), Ancylostoma sp (37,59%), Cestoda (19,55%) dan Capillaria sp (0,75%). Untuk itu diperlukan program pemberantasan dan pencegahan terhadap infeksi kecacingan pada kucing di Bali.
Ekstrak Daun Singkong Berpotensi Sebagai Antioksidan pada Burung Puyuh yang Mendapat Cekaman Panas Singkat La Jumadin; Aryani Sismin Satyaningtijas; Zulvia Maika; Lili Darlian; Warysatul Ummah; Koekoeh Santoso
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The purpose of this study was to explore the potential of cassava leaves extract as an antioxidant in quail (Coturnix coturnix japonica) females as a result of heat exposure. The parameter are total erythrocyte count, hematocrit value, hemoglobin concentration and erythrocyte index (MCV, MCH, MCHC). Quails were devided into six groups. The control group (K0) was not given cassava leaves extract and heat. Treatment group (KL) was only given chlorophyll cassava leaves extract dose 5,29 mg/g. Group P was only given heat exposure. Group P+KL1, P+KL2, and P+KL3 were exposed to heat and given the doses of cassava leaves extract 5,29 mg, 10,58 mg, and 21,16 mg/168 g body weight, respectively orally for 28 days after a week of adaptation. Parameters such as the total erythrocyte count, hematocrit value, hemoglobin concentration and erythrocyte index (MCV, MCH, MCHC) were observed every two weeks. Results showed that the mean of eritrosit ranged from 8,64 x 106/mm3 in K0 to 10,47 x 106/mm3 in P+KL3. Hematocrit value in all treatment groups did not increase. Temperature exposure and paste of cassava leaves with different level of dosages were not significantly different from MCV (P>0,05). The highest level of MCV was obtained in treatment K0 (0,48 fl). The highest level of MCH ranged from 5,83-6,33 pg. The highest level of MCH was obtained in treatment P (6,33 pg). The highest level of MCHC was obtained in treatment P (63,38 %). The mean of MCHC level in quail treated with the paste of cassava leaves tended to decrease compared to those were not treated with the paste. Conclusion of this study is cassava leaf extract has potential as an antioxidant in adult quail given short heat exposure. The application of chlorophyll extracted from cassava leaves did not significantly affect the quantity of blood cells, haemoglobin level, hematocrit value and eritrosite index in adult quail treated by short thermal stress.
Tingkat Proliferasi Primordial Germ Cells secara In Vitro dalam Medium Kultur dengan Penambahan Leukemia Inhibitory Factor (IN VITRO PROLIFERATION RATE OF MICE PRIMORDIAL GERM CELLS IN THE CULTURE MEDIUM WITH ADDITION OF LEUKEMIA INHIBITORY FACTOR) Wahono Esthi Prasetyaningtyas; Ni Wayan Kurniani Karja; Srihadi Agungpriyono; Mokhamad Fahrudin
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.304 KB) | DOI: 10.19087/jveteriner.2019.20.4.526

Abstract

Primordial germ cells (PGCs) are precursors for gamete cells. The totipotency of PGCs allows them to be used as a model for studying cancer and infertility. The study aimed to examine the characteristics of the mice fetus as a source of PGCs, proliferation rate of PGC and the role of LIF in vitro culture of PGCs. This study used genital ridges from 26 fetuses at 13.5 days post-coital (dpc) to isolate the PGCs. Genital ridges dissociation using 0.1% of trypsin and in vitro culture was carried out using the Dulbecco Modified Eagle Medium (DMEM) and incubated at 37 0C and 5% CO2 atmosphere. The fetus was measured and weighed to determine the normal development of the fetus and continued with the identification of the genital ridges after laparotomy performed under a stereomicroscope. Proliferation rate was measured by calculating Population Doubling Time (PDT), and cell viability was observed after in vitro culture for six days. The effect of adding 1000 IU/ml of leukemia inhibitory factor (LIF) was evaluated from two types of treatment in the medium, 1) DMEM added with 15% of fetal calf serum (FCS) (DMEM + S15%) and 2), DMEM was supplemented with 15% of FCS and 1000 IU/ml LIF (DMEM + S15% + LIF1000 IU/ml). Immunohistochemistry staining was carried out on the third-, sixth- and ninth-day of culture to detect the expression of Oct-4 in the PGCs, then cells were counted. The results showed that the fetus as a source of PGCs had normal development. The fetal sizes were 11 mm, and male and female genital ridges could be distinguished by morphology at the age of 13.5 dpc. The proliferation of PGCs was relatively slow with a 1.3 day PDT value with the viability of around 85%. Culture of PGCs with DMEM + S15% treatment showed the percentage of PGCs that expressing Oct-4 decreased from the third day of culture to the ninth day of culture. The culture of PGCs in DMEM + S15% + LIF 1000 IU / ml treatment showed that the percentage of PGCs that expressed Oct 4 increased on the sixth day of culture and decreased on the ninth day of culture. It can be concluded that the addition of LIF can maintain the number of PGCs until the sixth day of culture. LIF is thought to play a role in the regulation of proliferation of PGCs through receptors of LIF (RLIF) and glicoprotein (gp) 130 receptors.
PHYLOGENETIC AND ANTIGENIC STRUCTURE OF AVIAN INFLUENZA VIRUS OF H5N1 SUBTYPE ISOLATED FROM WATERFOWLS R Susanti; Retno Damajanti Soejoedono; I Gusti Ngurah Kade Mahardika; I Wayan Teguh Wibawan; Maggy Thenawidjaja Suhartono
Jurnal Veteriner Vol 9 No 3 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (542.593 KB)

Abstract

A study was carried (1) to analyze the phylogenetic relationship of fragment hemaglutinin (HA) geneof avian influenza viruses (AIV) subtype H5N1 isolated from apparently healthy backyard waterfowls inWest Java with representative of animal and human isolates from Indonesia and some countries in Asia;(2) to find out cross-reactivity of those viruses with a standard Indonesian strain. Nucleotide sequences ofHA gene of AIV H5N1 from backyard waterfowls along with other H5N1 isolates of Indonesian and Asianorigin were aligned using with ClustalW of MEGA 3.1 program. Estimation of genetic distance and theconstruction phylogenetic tree were conducted by Neighbor Joining method and calculation of distancematrix using Kimura 2-parameter. Antigenic analysis was conducted using hemagglutination inhibition(HI) test. Result of phylogenetic analysis indicated that all viruses from backyard waterfowls form threedistinct sublineages. One lineage was located in Indonesia cluster and two lineages in Asia cluster. In thephylogenetic analysis, it was concluded that multiple introductions of AIV H5N1 to Indonesia have occurred.Six AI H5N1 viruses from backyard waterfowls (IPB1-RS to IPB6-RS) appeared to be different ancestorsthose isolated previously in Indonesia. Cross-antigenic analysis showed that nine viruses isolates used inthis study were antigenically different to Legok 2003 chicken strain of AIV H5N1. The HI titer of anti-Legok 2003 antibody with all newly isolated viruses is up to 6 log lower then the HI titer using homologstrain.
Kecernaan In Vitro Ransum Berbasis Rumput Kumpai (Hymenachne acutigluma) Fermentasi Disuplementasi Legum Berbeda(IN VITRO DIGESTIBILITY OF FERMENTED HYMENACNE ACUTIGLUMA-BASED RATIONS SUPPLEMENTED WITH DIFFERENT LEGUMES) Riswandi Riswandi; Langgeng Priyanto; Afnur Imsya; Meilia Nopiyanti
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (113.472 KB) | DOI: 10.19087/jveteriner.2017.18.2.303

Abstract

The aim of this research was to study the effects on different supplementation legumes in the digestibility of fermented kumpai grass (Hymenachne acutigluma) based rations through in vitro technique. This study was conducted in Animal Feed and Nutrition Laboratory of Agriculture Faculty, Sriwijaya University. This study was done in 3 months. A completely randomized design with four treatments and four replicates was used in this study. The treatments were R0= 70% fermented kumpai grass + 30% concentrate + 0% legume, R1= 55% fermented kumpai grass + 7,5% lamtoro leaves + 7,5% water mimmosa + 30% concentrate, R2= 55% fermented kumpai grass + 7,5% acacia leaves + 30% concentrate , and R3=55% fermented kumpai grass + 5% lamtoro leaves + 5% acacia leaves + 5 % water mimmosa + 30% concentrate. Variables measured were dry matter digestibility, organic matter digestibility, volatile fatty acid (VFA), N-ammonia and pH. The result indicated that The adding of different legumes in the ration significantly (P<0.05) affected the dry matter digestibility, organic matter digestibility, N-ammonia and VFA. Duncan Multirange Range Test showed that treatment of control (R0) had the highest digestibility in dry matter (65,88%) and organic matter (65,34 %). The highest content of N-Ammonia, VFA was obtained in the treatment of adding lamtoro, acacia and water mimmosa (R3), namely 11 mM N-ammonia, and 158 mM VFA. It was concluded that the treatment R3 with adding lamtoro, acacia and water mimosa had the lowest digestibility but increased the ammonia and VFA of ration. ABSTRAK Tujuan penelitian ini adalah untuk mempelajari pengaruh suplementasi legum yang berbeda pada ransum berbasis rumput kumpai terhadap nilai kecernaan in vitro. Penelitian dilaksanakan selama tiga bulan di Laboratorium Nutrisi dan Makanan Ternak Fakultas Pertanian, Universitas Sriwijaya. Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan empat perlakuan dan empat ulangan. Masing-masing perlakuan adalah Perlakuan R0 = Ransum Kontrol (70% rumput kumpai fermentasi + 30% Konsentrat + 0% leguminosa (kontrol), R1 = 55% rumput kumpai fermentasi + 7,5% lamtoro + 7,5% kemon air + 30 % Konsentrat, R2 = 55% rumput kumpai fermentasi + 7,5% daun akasia + 7,5% kemon air + 30 % Konsentrat, R3 = 55% rumput kumpai fermentasi + 5% lamtoro + 5% kemon air + 5% daun akasia + 30 % konsentrat. Peubah yang diamati adalah koefisien cerna bahan kering (KCBK), koefisien cerna bahan organik (KCBO), N-Amonia,Volatile Fatty Acid (VFA), dan pH. Hasil penelitian memperlihatkan bahwa perlakuan berpengaruh nyata (P<0,05) terhadap KCBK, KCBO), N-Amonia dan VFA, sedangkan pH tidak nyata (P>0,05). Hasil uji lanjut menunjukkan bahwa perlakuan R0 memiliki nilai kecernaan bahan kering dan bahan organik tertinggi; KCBK 65,88 %, KCBO = 65,34 %. Kandungan N-Amonia dan VFA tertinggi terdapat pada perlakuan R3 yaitu N-Amonia: 11,00 mM dan VFA: 158 mM. Simpulan dari penelitian ini adalah penambahan kombinasi daun lamtoro, kemon air, dan akasia dapat menurunkan nilai KCBK, KCBO, sedangkan kadar N-Amonia dan VFA terjadi peningkatan.

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