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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
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Ekstrak Kulit Buah Manggis (Garcinia mangostana Linn) Mampu Meminimalkan Efek Imunosupresif Monosodium Glutamate (EFFECTIVENESS OF MANGOSTEEN PEEL EXTRACT (GARCINIA MANGOSTANA LINN) IN MINIMIZING THE IMMUNOSUPPRESSIVE EFFECT OF MONOSODIUM GLUTAMATE) Anak Agung Bagus Bramardipa; Anak Agung Ayu Mirah Adi; I Gusti Agung Arta Putra
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (564.491 KB) | DOI: 10.19087/jveteriner.2019.20.2.211

Abstract

Monosodium glutamate (MSG) is commonly used as a food additive or flavoring worldwide, unfortunately MSG is reported to have a toxic effect to the immune system, that can lead to an immunosuppressive conditions. One of immunosuppressive signs in chickens is atrophy of bursa Fabricius (BF). Mangosteen peel extract contains active substances and other components that can act as an immunomodulator. The aim of this study was to evaluate the immunosuppressive effects of MSG and the effectiveness of mangosteen peel extract in minimizing the immunosuppressive effects of the MSG. This study used a completely randomized design with four treatments namely A, B, C and K consisted four chickens each. The chickens in treatment A, B, C and K were given feed containing MSG 20% W/W and mineral water for drinking, commercial feed and mineral water containing 2% W/V mangosteen peel extract, MSG 20% W/W and mineral water containing mangosteen peel extract 2% W/V and commercial feed and drinking water without mixture. (control), respectively. Based on the results of this study, it was found that the administration of feed containing 20% MSG could result in bF atrophy with a very low bF index (p <0.01) if compared with B, C, and K-treatment. Prominet histopathological (HP) features of bF of chickens under treatment A are follicular atrophy and interfollicular edema. Treatment with feed containing 20% MSG along with the administration of drinking water containing 2% mangosteen peel extract keeps on maintaining the bF under normal condition even microscopically was found macrophages proliferation inside the lymphoid follicle. Based on clinical symptoms, index of bF and histopathological features, it can be concluded that MSG has immunosuppressive effects on chicks and mangosteen peel extract in drinking water can minimize this effect.
Pemakaian Duddingtonia flagrans dan Saccharomyces cerevisiae dalam Mereduksi Larva Infektif Haemonchus contortus (THE STUDY OF DUDDINGTONIA FLAGRANS AND SACCHAROMYCES CEREVISIAE USE ON REDUCING OF INFECTIVE HAEMONCHUS CONTORTUS LARVAE) Riza Zainuddin Ahmad; Fadjar Satrija; Nampiah Sukarno; Fachriyan Hasmi Pasaribu
Jurnal Veteriner Vol 13 No 1 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.832 KB)

Abstract

The use of Duddingtonia flagrans as the biological control of nomatode infections has been widelyreported. However, no report is available on the use of yeast Saccharomyces cerviciacae for such purpose.The aim of this study was to ivestigate the use of both fungi to reduce the number of Heamoncus contortusinfective larvae. Agar and fecal media containing the spore of the fungi was inoculated with infected H.contortus larvae (3rd stage). Fecal media containing the fungi was prepared by oral inoculation of sheepwith liquid containing 106, 107 spores of D. flagrans, and 106, 107 spores of D. flagrans, and 106, 1012 sporesof S. cerviciae. The number of larvae trapped in the fungi was counted. The result showed both fungi wereable to reduce the number of infective lave. However, for D. flagrans, beside it able to kill the larvae, it alsoable to trap the larva which did not occur in S. cerviceae. The combination of both fungi can be used to reduceof the number of invected H. contortus larvae.
Kekerabatan Genetik Caplak Rhiphicephalus (Boophilus) microplus Asal IndonesiaBerdasarkan Sekuen Internal Transcribed Spacer-2 (GENETIC RELATIONSHIP INDONESIAN RHIPHICEPHALUS (BOOPHILUS) MICROPLUS TICK BASED ON INTERNAL TRANSCRIBED SPACER-2 SEQUENSE ) Ana Sahara; Joko Prastowo; Rini Widayanti; Kurniasih .; Wisnu Nurcahyo
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.228 KB)

Abstract

Rhiphicephalus (Boophilus) microplus is important obligatory blood feeding ectoparasites transmittingmany different viral, bacterial and protozoan and plays a role as a vector of Babesia sp., The leria sp. andAnaplasma sp. in cattle. The accuracy in identifying and distinguishing interspecies and intraspeciesdiversity among parasites is needed to understand the epidemiology, biology and capacity as a vector.Variations in the DNA base sequence of the internal transcribed spacer region2 (ITS 2) has been used asa molecular marker for identification in an effort to determine phylogenetic relationships. The aim of thisstudy was to determine the ITS 2 gene nucleotide sequence of R. microplus, which was expected to beuseful for accurate identification the parasite diversity and phylogenetic relationship among many differentspecies. DNA amplification was conducted using BOO2 forward dan BOO2 reverse primers. The DNAsamples containing ITS2 region fragment of 1099 nt were derived from the nucleotide sequence multiplealignments of R.microplus and other ticks genes obtained from Gene bank using Clustal W software, andthen analyzed using the MEGA program version 6. Genetic distances based on nucleotide sequence weredetermined with Kimura 2-parameter method producing the smallest genetic distance of 0 % and 1.2 %.Construction of phylogenetic trees using the Neighbor joining method showed that ticks from variousregions in Indonesia was species complex which have a closer with R.microplus isolates from India, Laos,South Africa, China and Australia R.australis origin.
Anjing Kintamani sebagai Model Pada Penelitian Biomedik: Aspek Hematologi (THE KINTAMANI DOGS AS A MODEL FOR BIOMEDICAL RESEARCH : HEMOTOLOGICAL ASPECTS) I Ketut Puja
Jurnal Veteriner Vol 1 No 1 (2000)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1799.079 KB)

Abstract

Anjing Kintamani sebagai Model Pada Penelitian Biomedik: Aspek Hematologi (THE KINTAMANI DOGS AS A MODEL FOR BIOMEDICAL RESEARCH : HEMOTOLOGICAL ASPECTS)
Case of Entamoebiasis in Pigs Raised with a Free Range Systems in Bali, Indonesia (KASUS ENTAMOEBIASIS PADA BABI YANG DIPELIHARA DENGAN CARA DIUMBAR DI BALI, INDONESIA) Kadek Karang Agustina; Anak Agung Gde Oka Dharmayudha; Ida Bagus Made Oka; I Made Dwinata; I Made Kardena; Nyoman Sadra Dharmawan; I Made Damriyasa
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (125.329 KB)

Abstract

This research aims were to measure the prevalence of Entamoeba in pigs in Bali and to identify thezoonotic potential species of Entamoeba. A total of 183 pig stool samples from Bali have been examined.The method being used in this study were combination between coproscopy and molecular techniques.Concentration sedimentation with Sodium Acetic Formaldehide (SAF) as a solution was used in thecoproscopy method, while the Polimerase Chain Reaction method was used to amplify DNA of Entamoeba.Extracted sample’s DNA examined by using primers that specifically for Entamoeba: Entam 1 (F) (5’-GTTGAT CCT GCC AGT ATT ATA TG-3’) and Entam 2 (R) (5’-CAC TAT TGG AGC TGG AAT TAC-3), and toidentify the zoonotic potential species of Entamoeba, samples that produce 550 bp in first amplificationcontinued by primers Epolecki1 (F) (5’-TCG ATA TTT ATA TTG ATT CAA ATG-3’) and Epolecki2 (R) (5’-CCT TTC TCC TTT TTT TAT ATT AG-3’). The results showed that 76.6% of samples were positive incoproscopical examination, but 84.7 % produced 550 bp bands on PCR amplification by using generalprimers. All positive samples on the first PCR continued to second PCR used specific primers for E.poleckii as a potential zoonotic disease and all of the samples showed negative results. This datademonstrated that the prevalence of Entamoeba in a traditional pig scavenging systems in Bali was 84.7%but no specific infection infection caused by E. polecki was found.
Profil Protein Trypanosoma evansi dari Daerah Geografis Berbeda di Indonesia Tahun 2012-2014 dengan Sodium Dodecil Sulphate Polyacrylamide Gel Electrophoresis (TRYPANOSOMA EVANSI PROTEIN PROFILE OF DIFFERENT GEOGRAPHICAL AREAS ORIGIN IN INDONESIA Fitrine Ekawasti; Ichwan Yuniarto; Sulinawati Sulinawati; Didik Tulus Subekti
Jurnal Veteriner Vol 18 No 4 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.624 KB) | DOI: 10.19087/jveteriner.2017.18.4.526

Abstract

Surra outbreak in 2012 has led to more than 1,700 animals have died in the province of East Nusa Tenggara (NTT) Indonesia. Surra case sporadically continues throughout the year in various areas, especially Kalimantan, Banten as well as other areas. Some reports reveal differences in protein profiles among multiple isolates of T. evansi. Therefore the purpose of this research were to find out the protein profile of each isolate T. evansi in Indonesia and the possible biological differences among them. Eleven isolates originating from the province of East Nusa Tenggara, South Kalimantan and Central Kalimantan, Banten, Lampung and Bengkulu has been isolated and purified Using DEAE. Trypanosoma isolate were frezeethawing repeatedly to obtain soluble protein. Furthermore, soluble protein is treated with heating or without heating and then each was run on SDS PAGE with Coomassie Blue staining. The protein profiles of all isolates were compared each other. The results showed that eleven isolates of T. evansi in Indonesia has a very diverse protein profile. Then for the purposes of development of diagnostic kit can be used whole lysate cell (WCL) as stock antigen in serological test process.
Variasi Genetik Populasi Monyet Ekor Panjang (Macaca fascicularis) di Pulau Nusa Penida, Klungkung, Bali (GENETIC VARIATION OF LONG TAIL MACAQUE (MACACA FASCICULARIS) POPULATION IN NUSA PENIDA ISLAND, KLUNGKUNG, BALI) Elisabeth Yulia Nugraha; I Nengah Wandia; I Gede Soma
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (118.91 KB) | DOI: 10.19087/jveteriner.2018.19.4.531

Abstract

The purpose of this study is to discover about heterozygosity, migration between populations, and mating habits of Macaca fascicularis among the population in Nusa Penida Island, Klungkung, Bali. Fourteen blood samples from Macaca fascicularis were taken from Nusa Penida Island that consist of eleven samples from Puncak Mundi Temple and three samples from Paluang Temple were used in the study. Samples were extracted using QIAamp DNA Blood Mini Kits produced by Qiagen. Five loci microsatellite (D10S611, D11S1366, D13S765, D17S1290, and D18S536) were amplified using the Polymerase Chain Reaction (PCR) technique. Thirty cycles of PCR were carried out with an annealing temperature of 500C. Alleles were separated by electrophoresis on eight percent polyacrylamide gel and presented with silver staining. The results showed high heterozygosity is 0.762 of Macaca fascicularis populations on Nusa Penida Island with random mating habits. Low genetic differentiation is 0.05. High the number of migrations between populations (genetic flow) of Macaca fascicularis populations on Nusa Penida Island is 4.75 with migration between populations of five individual/generation. Based on the results of the study it was concluded that the genetics of the Macaca fascicularis population on Nusa Penida Island varies.
Studi Kasus: Ascariosis Disertai Migrasi Larva pada Hati dan Paru-paru Babi Landrace (CASE STUDY: ASCARIOSIS ACCOMPANIED BY MIGRATION OF LARVAE IN LIVER AND LUNGS OF LANDRACE PIG) I Ketur Berata; Ida Bagus Oka Winaya; Anak Agung Ayu Mirah Adi; I Made Kardena; Ida Bagus Windia Adnyana
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (401.797 KB) | DOI: 10.19087/jveteriner.2019.20.4.603

Abstract

Cases of ascariosis due to infection with Ascaris suum are still prevalent in the world, including Indonesia. Apart from causing economic losses, ascariosis can also threaten human health because it is zoonotic. Case studies of ascariosis with typical white spot lesions in the landrace pig liver is described in this paper. Pigs aged 60 days old, sick for 10 days, thin and coughing gathered from the village of Suwung, Denpasar City. After necropsy and post mortem examination were done, tens of A. suum worms were found in the intestine and white spot lesions observed on the livers. Tissues of small intestine, large intestine, liver, lungs, kidney, spleen, urinary vesicles and brain were taken and put into 10% of formalin neutral buffer before they then processed in making histopathological preparations. Histopathological preparation was carried out using paraffin block method and hematoxylin eosin (HE) staining technique. The results of histopathological examination of the small intestine, large intestine, liver and lungs were found to be infiltrated by eosinophil cells. Eosinophil cells infiltration indicates that there are parasites A. suum or the larvae migrant in the tissues. Some of the tissues in livers were also found to have fibrosis, which is suspected that the infection has been chronic. It can be concluded that cases of the landrace pigs were chronically infected by A.suum and accompanied by migration of the larvae to the livers and lungs. More attention is needed to prevent the increase of ascariosis in pigs in order to minimize economic losses and transmission to humans.
REACTIVITY OF ANTI-CAPSID MONOCLONAL ANTIBODIES WITH NATIVE AD RECOMBINANT PROTEIN OF JEMBRANA DISEASE VIRUS REAKTIVITAS ANTIBODI MONOKLONAL ANTI-KAPSID DENGAN PROTEIN NATIF DAN PROTEIN REKOMBINAN VIRUS PENYAKIT JEMBRANA Nyoman Mantik Astawa
Jurnal Veteriner Vol 8 No 2 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Reactivity of anti-capsid monoclonal antibody (mAbs) with both native and recombinant protein of Jembrana Disease Virus (JDV) was examined. The monocloncal antibodies were produced by fusion of my.loma cells with lymphocytes of mice immunized with JDV antigen. Ten mAbs were produced and they designated as CC12, BB7, AH7, BD2, DB2, AF9, BC10, AG7, AH4 and CB11. In ELISA test, all mAbs reacted with recombinant proteins (glutation-8-transferase-capsid/GST-Ca and histidine tagged capsid/His-Ca) but not with GST alone.
Sekuensing 16S DNA Bakteri Selulolitik Asal Limbah Cairan Rumen Sapi Peranakan Ongole (SEQUENCING OF 16S DNA OF CELLULOLYTIC BACTERIA FROM BOVINE RUMEN FLUID WASTE ONGOLE CROSSBREED) Widya Paramita Lokapirnasari; Adriana Monica Sahidu; Tri Nurhajati; Koesnoto Supranianondo; Andreas Berny Yulianto
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.233 KB) | DOI: 10.19087/jveteriner.2017.18.1.76

Abstract

This study aimed to identified cellulolytic inoculant code WPL 214 isolated from bovine rumen fluid waste of Ongole Cross Breed of Surabaya Slaughter house. A single colony of isolates celulolytic grown on 5 mL of liquid media Luria Bertani (LB) consist of 1 % NaCl , 1% tripton , 0.5 % yeast extract, containing1 % carboxymethyl cellulose (CMC) at temperature 37°C, using a shaker of incubator during 16-18 hours. That isolate determined by 16S DNA gen analysis using High Fidelity Platinum Taq DNA Polymerase with primer forward PB36 5’-AGR GTT TGA TCM TGG CTC AG-3’ and primer reverse PB38 5’-GMT ACCTTG TTA CGA CTT-3’ for PCR. Nucleotide sequence of 16S DNA fragment was determined through the sequencing method. The result was then compared with GenBank database to recognize the type of the sample bacteria. DNA isolation and 16S DNA coding genes amplification were carried out using Kit High Fidelity Platinum Taq DNA Polymerase. Afterward, BLAST was applied to identify the phylogenetic tree. The bacteria was capable of indicating the existence of clear zone in a media CMC by congo red staining. The existence of the clear zone associated with the activity of microbes to degrade cellulose. The conclusión of this research based on the results was the sequencing nucleotides genome 16S DNA showed that cellulolytic inoculant was identified as Enterobacter cloacae WPL 214. ABSTRAK Penelitian ini bertujuan untuk mengidentifikasi lebih lanjut isolat selulolitik kode WPL 214 yang telah diisolasi dari cairan rumen sapi peranakan ongole dari limbah Rumah Potong Hewan Surabaya. Koloni tunggal dari isolat selulolitik ditumbuhkan pada 5 mL media cair Luria Bertani (LB) dengan komposisisi 1% NaCl, 1% tripton, 0,5% yeast ekstrak, yang mengandung 1% substrat carboxymethyl cellulose (CMC) pada suhu 37°C, dengan pengocokan menggunakan shaker incubator selama ±16-18 jam. Penelitian ini terdiri dari dua tahap, tahap pertama dilakukan isolasi DNA, tahap kedua dilakukan identifikasi gen penyandi 16S DNA, amplifikasi DNA dengan polymerase chain reaction (PCR). Amplifikasi gen penyandi 16S DNA menggunakan Kit High Fidelity Platinum Taq DNA Polymerase dengan primer forward PB36 5’-AGR GTT TGA TCM TGG CTC AG-3’ dan primer reverse PB38 5’-GMT ACC TTG TTA CGA CTT-3’ yang digunakan untuk PCR. Hasil sekuensing nukleotida dari 16S DNA selanjutnya dibandingkan dengan urutan nukleotida dari GenBank database untuk dilakukan BLAST untuk mengidentifikasi berdasarkan pohon filogeni. Bakteri tersebut mampu menunjukkan adanya zona bening pada media Carboxymethyl cellulose (CMC) dengan pewarnaan congo red. Adanya zona bening tersebut berhubungan dengan aktivitas mikrob untuk mendegradasi selulosa. Simpulan penelitian menunjukkan bahwa berdasarkan hasil urutan nukleotida genom 16S DNA serta pohon filogeni, maka isolat selulolitik tersebut diidentifikasi sebagai Enterobacter cloacae WPL 214.

Page 43 of 112 | Total Record : 1116

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