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All Journal Jurnal Kedokteran Syiah Kuala Indonesian Journal of Cancer Chemoprevention JURNAL FARMASIMED (JFM) Indonesian Journal of Pharmaceutical and Clinical Research International Journal Of Science, Technology & Management (IJSTM) Unri Conference Series: Community Engagement
Hasibuan, Poppy Anjelisa Zaitun
Universitas Sumatera Utara
Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Public Health Transportation

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Journal : JURNAL FARMASIMED (JFM)

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Cytotoxic Potential of Extract Methanol and Fraction of African Leaf (Vernonia amygdalina Del.) on B16F10 Cell Line in Melanoma Cancer Risqina Lubis, Sahwani; Hasibuan, Poppy Anjelisa Zaitun; Satria, Denny
Jurnal FARMASIMED (JFM) Vol 8 No 2 (2026): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/dyf31640

Abstract

Melanoma is a highly aggressive malignancy derived from melanocytes and is considered the most severe form of skin cancer. Natural products have increasingly attracted attention as potential sources for the discovery of novel anticancer agents. The purpose of this study is to assess the cytotoxic activity of Vernonia amygdalina Delile leaf methanol extract and chloroform fraction against B16F10 melanoma cells. Vernonia amygalina Del. Leaves extracted using reflux method and liquid-liquid fractionation. Identification of the contained compounds were identified using thin layer chromatography. B16F10 cells were cultured under standard conditions and treated with various concentrations of the chloroform fraction and methanol extract of Vernonia amygdalina Delile leaves and the cytotoxicity assay was performed using the MTT method to determine cell viability and IC₅₀ values. The analysis revealed IC₅₀ values of chloroform fraction (81.80±4.18) and followed by n-hexane fraction (187.33±12.48), etil acetate fraction (204.35±8.39), methanol extract (344.37±25.12) and residual fraction (4032.38±921.22). All fraction and the methanol extract demonstrated dose-dependent cytotoxicity, with the chloroform fraction exhibiting a more pronounced inhibitory effect. These results suggest that Vernonia amygdalina leaves contain bioactive constituents with significant anticancer potential against melanoma. Future studies should focus to the isolation of active compounds and comprehensive in vivo validation to strengthen the therapeutic prospects.
Anticancer Potential of Methanol Extract and Fractions of African Leaf (Vernonia amygdalina Del.) Against HeLa Cervical Cancer Cells Suryati Cintia Gultom; Poppy Anjelisa Zaitun Hasibuan; Denny Satria
Jurnal FARMASIMED (JFM) Vol 8 No 2 (2026): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/yd7znx87

Abstract

Globally, cervical cancer is one of the leading causes of cancer-related mortality among women. African leaves (Vernonia amygdalina Del.) are known to contain active terpenoid compounds, such as sesquiterpene lactones, which can be utilized for cancer therapy. The aim of this study is to evaluate the cytotoxic potential of methanol extracts and leaf fractions from the African plant as anticancer agents against the HeLa cervical cancer cell line in vitro. The crude African leaf extract was prepared using a reflux method with methanol, followed by liquid-liquid fractionation to obtain n-hexane, chloroform, ethyl acetate, and residual fractions. Cytotoxicity testing was performed using the MTT Assay on HeLa cervical cancer cell lines; samples exhibiting the strongest cytotoxic activity underwent further testing, including cell cycle analysis and apoptosis assays using flow cytometry. The cytotoxicity test results showed that the methanol extract and fractions from African leaves exhibited cytotoxic effects with IC50 values ranging from 25.45 to 4163.63 μg/mL, with the chloroform fraction (IC50 = 25.45 ± 0.97 μg/mL) demonstrating the strongest cytotoxic activity. Cell cycle assay results showed that the chloroform fraction could inhibit HeLa cell proliferation in the G0-G1 phase. Cell apoptosis results indicated that the chloroform fraction could increase total apoptosis by 3.4%. Therefore, African leaves have potential as anticancer candidates, with anticancer active compounds tending to be concentrated in the chloroform fraction. Therefore, African leaves show potential as anticancer agents, with the active anticancer compounds tending to be concentrated in the chloroform fraction. Further studies are urgently needed to isolate and identify the pure compounds from the chloroform fraction of African leaves.
Co-Authors . Marianne Angkat, Robbani Syahfitri Bestari, Khansa Candra Denny Satria Denny Satria Denny Satria, Denny Ginting, Junius Gian Hanum, Tengku Ismanelly Harefa, Herman Syukur Haro, Ginda Hartini, Putri Tri Hasibuan, Wan Fadilla Hendri Asrin Illian, Didi Nurhadi Ilyas , Syafruddin Irma Yanti Rangkuti Juwita, Nur Aira lubis, muhammad fauzan M. Ichwan Mardiana Marianne Marianne Nasution , Pandapotan Nasution, Pandapotan Panal Sitorus Prayoga, Andre Risqina Lubis, Sahwani Rizka Damela Sibuea Rosidah Rosidah Rosidah Said Aldi Palevi Siregar, Dedek Harianti Sitanggang, Rosa Gloria Suci, Nurul Suharyanisa Sumaiyah Sumaiyah, Sumaiyah Suryati Cintia Gultom Tri Widyawati Urip Harahap Yuandani