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All Journal Journal of Food and Pharmaceutical Science Pharmaciana: Jurnal Kefarmasian Majalah Obat Tradisional Abditani : Jurnal Pengabdian Masyarakat Jurnal Karinov JUNCTO: Jurnal Ilmiah Hukum Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences Jurnal Ilmiah Medicamento COMSERVA: Jurnal Penelitian dan Pengabdian Masyarakat Jurnal Locus Penelitian dan Pengabdian Usadha
Putra, I Made Agus Sunadi
Program Studi S1Fakultas Farmasi Universitas Mahasaraswati Denpasar
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Public Health Social Sciences

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Uji Aktivitas Antibakteri Sediaan Gel Hand Sanitizer Ekstrak Etanol Daun Kaktus Pakis Giwang (Euphorbia milli) Terhadap Bakteri Pseudomonas aeruginosa Putra, I Made Agus Sunadi; Putri, Ni Putu Devi Nirmala; Santoso, Puguh
Usadha Vol 4 No 2 (2025): Usadha : Jurnal Integrasi Obat Tradisional
Publisher : Fakultas Farmasi Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36733/usadha.v4i02.7434

Abstract

The Covid-19 pandemic has heightened public awareness of hand hygiene thus creating a demand for safe and effective natural-based hand sanitizers. This study aimed to determine the antibacterial activity of hand sanitizer gel containing ethanol extract of Euphorbia milli leaves against Pseudomonas aeruginosa. The extract was obtained through maceration of 500 g of powdered leaves in 70% ethanol and formulated into gels with concentrations of 5%, 10%, and 15% using CMC-Na, glycerin, propylene glycol, and methyl paraben as base ingredients. Physical quality tests included organoleptic properties, homogeneity, pH, and spreadability, while antibacterial activity was assessed using the disc diffusion method on Mueller Hinton Agar. All formulations showed brown colour, homogeneous texture, and pH averaging 5.5, meeting standard requirements. The spreadability ranged between 5.0–6.0 cm, indicating good application consistency. Inhibition zone diameters against P. aeruginosa increased with extract concentration: 6.52 ± 0.26 mm (5%), 7.51 ± 0.30 mm (10%), and 8.39 ± 0.35 mm (15%), whereas the positive control (Ciprofloxacin 5 µg) produced 29.92 ± 1.06 mm and the negative control showed 0 mm. Based on inhibition zone classification, all formulations exhibited moderate antibacterial activity. In conclusion, the ethanol extract of Euphorbia milli leaves demonstrates antibacterial potential against Pseudomonas aeruginosa, with effectiveness increasing proportionally to concentration, suggesting its potential as a natural active ingredient for hand sanitizer formulations
Production of Solid Soap from Arabica Coffee Grounds (Coffea arabica L.) with Antibacterial Properties Sanjiwani, Ni Made Sukma; Ariani, Komang; Sunadi Putra, I Made Agus; Rahadi, I Wayan Surya; Mirah Mariati, Ni Putu Ayu; Sudiarsa, I Wayan; Udayani, Ni Nyoman Wahyu
Journal of Food and Pharmaceutical Sciences Vol 14, No 1 (2026): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.25035

Abstract

Coffee shops are now a favourite hangout for people of all ages, so many entrepreneurs are developing coffee shop businesses because of their high profit potential. Arabica coffee is the type of coffee most commonly used in coffee shops. The coffee-making process produces waste in the form of coffee grounds. Coffee grounds can be used as an ingredient in beauty products such as soap. Soap is the result of saponification, which is a reaction between a base or alkali and fatty acids, which acts as a skin cleanser. This study aims to examine the presence of secondary metabolite compounds in Arabica coffee grounds (Coffea arabica L.), test the physical quality of solid soap made from Arabica coffee grounds (C arabica L.), and assess the soap's ability to inhibit the growth of Staphylococcus aureus bacteria. Arabica coffee grounds were first analysed through qualitative phytochemical screening tests using test tubes and various reagents, then formulated into solid soap with different concentrations, namely 4%, 7%, and 9%. After that, physical properties testing and antibacterial activity evaluation against Staphylococcus aureus were carried out using the disc diffusion method. The research results data were presented using descriptive analysis. The tests revealed that Arabica coffee residues contain secondary metabolites in the form of alkaloids, flavonoids, tannins, saponins, and triterpenoids. Solid soap made from Arabica coffee grounds meets the physical quality test standards in accordance with SNI 3532:2021 and that solid soap made from Arabica coffee grounds with a percentage of 7% (formula 2) and 9% (formula 3) has the potential to inhibit the activity of Staphylococcus aureus bacteria with a moderate category. It can be concluded that Arabica coffee grounds contain secondary metabolites such as alkaloids, flavonoids, tannins, saponins, and triterpenoids. Solid soap with Arabica coffee grounds as an ingredient meets the physical quality requirements in accordance with SNI 3532:2021. Soap formulas with a coffee grounds concentration of 7% (F2) and 9% (F3) have the potential to inhibit the growth of Staphylococcus aureus with moderate efficacy.
Co-Authors Agung Nur Cahyanta Ariani, Komang Danayasa, I Kadek Debby Juliadi Eko Mugiyanto Erna Cahyaningsih Herleeyana Meriyani I Gusti Ayu Putri Kartika, I Gusti Ayu I Gusti Ngurah Parikesit Widiatedja I Putu Tangkas Suwantara I WAYAN SUDIARSA Ika Widi Astuti, Ika Widi Ketut Agus Adrianta Megawati, Fitria Nggaba, Ellenoire Rambu Kabeba Hammu Ni Luh Kade Arman Anita Dewi Ni Made Dwi Mara Widyani Nayaka Ni Made Sukma Sanjiwani Ni Nyoman Wahyu Udayani Ni Putu Agustini Ari Dewi NI PUTU AYU MIRAH MARIATI Ni Putu Leona Yorita Dewi Ni Putu Udayana Antari Partomuan Simanjuntak Puguh Santoso puguh santoso, puguh Putra Hilmi Prayitno Putri, Ni Putu Devi Nirmala Putu Era Sandhi Kusuma Yuda Rahadi, I Wayan Surya Riastini, Ni Wayan Rika, Putu SARTIKAWATI, NI KADEK AYU Sasadara, Maria Malida Vernandes Siswa Setyahadi Sri Budiasih, Putu Eka Wardani, I Gusti Agung Ayu Kusuma Yudianti Mendra, Ni Nyoman Yuwono, Moch.