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The increasing of enamel calcium level after casein phosphopeptideamorphous calcium phosphate covering Widyasri Prananingrum; Puguh Bayu Prabowo
Dental Journal (Majalah Kedokteran Gigi) Vol. 45 No. 2 (2012): June 2012
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Caries process is characterized by the presence of demineralization. Demineralization is caused by organic acids as a result of carbohydrate substrate fermentation. Remineralization is a natural repair process for non-cavitated lesions. Remineralization occurs if there are Ca2+ and PO43- ions in sufficient quantities. Casein-amorphous calcium phosphate phosphopeptide (CPP-ACP) is a paste material containing milk protein (casein), that actually contains minerals, such as calcium and phosphate. The casein ability to stabilize calcium phosphate and enhance mineral solubility and bioavailability confers upon CPP potential to be biological delivery vehicles for calcium and phosphate. Purpose: The aim of this study was to determine the calcium levels in tooth enamel after being covered with CPP-ACP 2 times a day for 3, 14 and 28 days. Methods: Sample were bovine incisors of 3 year old cows divided into 4 groups, namely group I as control group, group II, III and IV as treatment groups covered with CPP-ACP 2 times a day. All of those teeth were then immersed in artificial saliva. Group II was immersed for 3 days, while group III was immersed for 14 days, and group IV was immersed for 28 days. One drop of CPP-ACP was used to cover the entire labial surface of teeth. The measurement of the calcium levels was then conducted by using titration method. All data were analyzed by One- Way ANOVA test with 5% degree of confidence. Results: The results showed significant difference of the calcium levels in tooth enamel of those groups after covered with CPP-ACP 2 times a day for 3, 14 and 28 days (p = 0.001). There is also significant difference of the calcium levels in tooth enamel of those treatment groups and the control group (p = 0.001). Conclusion: The calcium levels of tooth enamel are increased after covered with CPP-ACP 2 times a day for 3, 14 and 28 days.Latar belakang: Proses terjadinya karies gigi ditandai oleh adanya demineralisasi. Demineralisasi terjadi oleh asam organik sebagai hasil fermentasi substrat karbohidrat oleh bakteri. Remineralisasi adalah proses perbaikan alami untuk lesi non cavitated. Remineralisasi terjadi jika terdapat ion Ca2+ dan PO43- dalam jumlah cukup. Casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) adalah bahan berbentuk pasta berisi suatu protein susu (kasein). Pada kasein terkandung mineral kalsium dan fosfat. Kemampuan kasein untuk menstabilkan kalsium fosfat dan meningkatkan kelarutan dan bioavailabilitas sehingga CPP memiliki potensi menghantarkan kalsium dan fosfat. Tujuan: Tujuan penelitian ini untuk mengetahui kadar kalsium enamel setelah pengulasan CPP-ACP pada permukaan enamel 2 kali sehari selama 3,14 dan 28 hari. Metode: Sampel adalah gigi insisif sapi, usia 3 tahun. Pada penelitian ini sampel (n = 24) dibagi menjadi 4 kelompok yaitu kelompok I sebagai kelompok kontrol, dan kelompok II, III, IV sebagai kelompok perlakuan dengan pengulasan CPP-ACP 2 kali sehari. Semua kelompok direndam dalam saliva buatan. Kelompok II direndam 3 hari, kelompok III direndam 14 hari, kelompok IV direndam 28 hari. Pengulasan CPP-ACP sejumlah 1 tetes diratakan pada seluruh permukaan labial gigi. Pengukuran kadar kalsium dilakukan dengan metode titrasi. Semua data dianalisa dengan uji One-Way ANOVA dengan taraf kemaknaan 5%. Hasil: Hasil penelitian menunjukkan perbedaan yang signifikan kadar kalsium enamel gigi di antara kelompok (p = 0,001). Hasil penelitian menunjukkan perbedaan yang signifikan kadar kalsium gigi pada kelompok kontrol dan perlakuan antara lama waktu pengulasan 3, 14, dan 28 hari (p = 0,001). Kesimpulan: Pengulasan CPP-ACP selama 3, 14 dan 28 hari mampu meningkatkan kadar kalsium pada enamel.

The increasing of odontoblast-like cell number on direct pulp capping of Rattus norvegicus using chitosan Widyasri Prananingrum
Dental Journal (Majalah Kedokteran Gigi) Vol. 43 No. 4 (2010): December 2010
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Pulpal perforation care with direct pulp capping in the case of reversible pulpitis due to mechanical trauma was performed with chitosan which has the ability to facilitate migration, proliferation, and progenitor cell differentiation. Purpose: The purpose of this study was to determine the increasing number of odontoblast-like cells in direct pulp capping dental care of Rattus norvegicus using chitosan for seven and fourteen days. Methods: Samples were molars of male Rattus norvegicus strain wistar, aged between 8–16 weeks, divided into two treatment groups, namely group I given chitosan and group II as a control group given Ca(OH)2. Those Rattus norvegicus’ occlusal molar teeth were prepared with class I cavity, and then chitosan and Ca(OH)2 were applied as the pulp capping materials. Afterwards, glasss ionomer cement type IX was used as a restoration material. Their teeth and jaw were then cut on the seventh day and the fourteenth day. Next, histopathological examination was carried out to observe the odontoblast like cells. All data were then analyzed by t test. Degree of confidence obtained, finally, was 95%. Results: The results obtained showed that the significant differences of odontoblast like cells on the seventh day observation was 0.001 (p = 0.001), and on the fourteenth day observation was 0.002 (p = 0.002). Conclusion: The number of odontoblast-like cells in direct pulp capping dental care of rattus norvegicus using chitosan is higher than the one using Ca(OH)2 for seven and fourteen days.Latar belakang: Perawatan perforasi pulpa pada kasus pulpitis reversible karena trauma mekanis bur dilakukan direct pulp capping dengan cara pemberian bahan secara topikal pada daerah perforasi. Kitosan memiliki kemampuan untuk memfasilitasi migrasi, proliferasi dan diferensiasi sel progenitor pulpa. Tujuan: Tujuan penelitian ini adalah untuk menentukan jumlah peningkatan odontoblas-like cell pada perawatan direct pulp capping gigi Rattus norvegicus menggunakan kitosan selama 7 dan 14 hari. Metode: Sampel adalah gigi molar Rattus norvegicus jantan strain wistar, berusia antara 8–16 minggu, dibagi menjadi 2 kelompok perlakuan yaitu kelompok I yang diberi kitosan dan kelompok II sebagai kontrol yang diberi Ca(OH)2. Oklusal gigi molar Rattus norvegicus dipreparasi kelas I kemudian kitosan dan Ca(OH)2 diaplikasikan sebagai bahan pulp capping. Glass ionomer cement tipe IX digunakan sebagai bahan restorasi. Gigi beserta rahang tikus dipotong pada 7 dan 14 hari. Pemeriksaan histopatologi dilakukan untuk mengamati odontoblas-like cell. Semua data dianalisis dengan uji t. Tingkat kepercayaan = 95%. Hasil: Hasil penelitian menunjukkan perbedaan yang signifikan dalam odontoblas like cell pada pengamatan hari ke-7 (p = 0,001) dan pengamatan hari ke 14 (p = 0,002). Kesimpulan: Jumlah odontoblas like cell pada perawatan direct pulp capping gigi Rattus norvegicus menggunakan kitosan lebih tinggi dibandingkan dengan Ca(OH)2 selama 7 dan 14 hari.

The effects of Anadara granosa shell-Stichopus hermanni on bFGF expressions and blood vessel counts in the bone defect healing process of Wistar rats Rima Parwati Sari; Sri Agus Sudjarwo; Retno Pudji Rahayu; Widyasri Prananingrum; Syamsulina Revianti; Hansen Kurniawan; Aisah Faiz Bachmid
Dental Journal (Majalah Kedokteran Gigi) Vol. 50 No. 4 (2017): December 2017
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Bone damage can be caused by various factors with treatment usually involving graft materials being applied to the defective area. Moreover, in the bone defect healing process, blood vessels are also considered to be an important energy source for cell proliferation. One of the angiogenic factors playing an important role in blood vessel formation is basic fibroblast growth factor (bFGF). Furthermore, synthesized hydroxyapatite derived from Anadara granosa (AG) shells constitutes one of the potential materials for use in bone graft. The gold sea cucumber genus Stichopus hermanni (SH) possesses the ability to stimulate endothelial progenitor cells inducing bFGF. Purpose: This study aims to investigate the effects of AG shells and SH on bFGF expressions and blood vessel counts within the bone healing process. Methods: Twenty four male Wistar rats were divided into three groups, namely: a control group (C), a treatment group was administered with blood cockle shell (AG), and a treatment group with blood cockle shell and golden sea cucumber (AG+SH). Defects were made on their femurs measuring half the diameter of a circular, no. 018. bur These rats were subsequently sacrificed on day 7 after surgery. The expressions of bFGF were measured by means of IHC technique, while the number of blood vessels was quantified using HE technique. The resulting data was subjected to statistical analysis using an Anova test followed by an LSD test (p < 0.05). Results: The one-way Anova test results combined with those of an LSD test showed there to be significant differences in bFGF expressions and blood vessel counts between the control group (K) and the treatment group (AG) as well as between the treatment group (AG) and the treatment group (AG+SH). Conclusions: A combination of Anadara granosa shell and Stichopus hermanni can increase the expression of bFGF and the number of blood vessels on day 7 during the bone healing process in Wistar rats.

The effect of various concentrations of HA-TCP derived from cockle shell synthesis on scaffold porosity Reyhan Alvaryan Ferdynanto; Priska Evita Setia Dharmayanti; Putu Tahlia Krisna Dewi; Widyasri Prananingrum
Dental Journal (Majalah Kedokteran Gigi) Vol. 51 No. 3 (2018): September 2018
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Porosity is an important property that must be possessed by scaffold due to its role in new bone growth. Hydroxyapatite is a scaffold material with a composition resembling that of bone that can be synthesized from cockle shell (Anadara granosa). Purpose: This research aimed to determine the effects of various HA-TCP concentrations (wt%) derived from cockle shell synthesis on scaffold porosity. Methods: HA-TCP was synthesized from cockle shells using a hydrothermal method at 200o C with a 12-hour sintering process period. An XRD test was subsequently carried out to determine the composition of hydroxyapatite (HA) and tricalcium phosphate (TCP) compounds. Eighteen scaffold samples (n=6) were then produced using a freeze dry method and divided into three groups, namely; Group 1 (K1) treated with 5% HA-TCP, Group 2 (K2) treated with 25% HA-TCP and Group 3 (K3) treated with 50% HA-TCP. Thereafter, a scaffold porosity test was conducted using liquid displacement method. Scaffold porosity was observed by means of an SEM image. A One-Way ANOVA test was subsequently performed, followed by an LSD Post-Hoc test (p <0.05). Results: The results of the XRD test showed that the percentage of HA was 51.5%, while TCP was 16.8%. The porosity of the scaffolds was within the range of 67.24% - 80.17%. The highest porosity was found in Group 1, while the lowest occurred in Group 3. There were significant differences in all groups. Conclusion: The concentration of HA-TCP derived from the synthesis of cockle shells affects the porosity of scaffold. The lower the concentration of HA-TCP, the higher the scaffold porosity.

Variations of gelatin percentages in HA-TCP scaffolds as the result of 6- and 12-hour sintering processes of blood cockle (Anadara granosa) shells against porosity Desak Putu Sudarmi Ari; Firda Dean Yonatasya; Gita Saftiarini; Widyasri Prananingrum
Dental Journal (Majalah Kedokteran Gigi) Vol. 51 No. 4 (2018): December 2018
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Porous scaffold is one type of biomaterial primarily employed as a bone substitute material which demonstrates superior osteoconductive and osteointegrative properties than solid scaffold since it can stimulate and accelerate the growth of new tissue. For the purposes of this study, porous scaffold was produced using hydroxyapatite-tricalcium phosphate (HA-TCP) powder derived from a synthesis of blood cockle (Anadara granosa) shells and gelatin. Purpose: The aim of this study was to reveal the effects of the percentage of gelatin in HA-TCP scaffolds derived from 6- and 12-hours sintering processes involving blood cockle shells on porosity. Methods: HA-TCP powder was derived from a synthesis of Anadara granosa shells using a hydrothermal method at 200oC with sintering periods of 6 and 12 hours. A XRD test was subsequently conducted to reveal the compositions of HA-TCP powder. The 24 scaffold samples (n=6) employed were manufactured using a freeze dry method before being divided into four groups, namely; Group 1 using 25% HA-TCP powder (a six-hour sintering process) combined with 20% gelatin, Group 2 using 25% HA-TCP powder (a six-hour sintering process) combined with 10% gelatin, Group 3 using 25% HA-TCP powder (a twelve-hour sintering process) combined with 20% gelatin; and Group 4 using 25% HA-TCP powder (a twelve-hour sintering process) combined with 10% gelatin. A scaffold porosity test was subsequently carried out using a liquid displacement method. A one-way ANOVA test was performed using SPSS, followed by a Post-Hoc LSD (p<0.05). Results: The statistical results for scaffold porosity were within the range of 67.21 -77.51%. The highest porosity was found in Group 3, while the lowest was in Group 4. Significant differences were also present in all groups. Conclusion: Variations in the percentage of gelatin can affect the porosity of HA-TCP scaffolds derived from 6-and 12 hours sintrering processes blood cockle shells. The smaller the percentage of gelatin, the higher the porosity.

The Effect of Calcium Compounds Bone Graft Synthesized from Blood Cockle Shells (Anadara granosa) with Sintering Time Variation in Fibroblast Proliferation on Socket Healing Process Firda Dean Yonatasya; Widyasri Prananingrum; Meinar Nur Ashrin
Denta Journal Kedokteran Gigi Vol 13 No 1 (2019): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar

Backgorund: Anadara granosa shells can be converted into calcium compounds in the form of hydroxyapatite (HA), tricalcium phosphate (TCP), and calcium carbonate (CaCO3) by hydrothermal method. These calcium compounds have the potential to increase the proliferation of fibroblast cells in the socket healing process. Purpose: The aim of this study was to determine the effect of calcium compounds from the synthesis of Anadara granosa with variations in sintering time against proliferation of fibroblast cells in socket healing. Materials and Methods: 36 Wistar rats were divided into control group (K1, K2), and treatment group (P1a, P1b, P2a, P2b) which was applied by bone graft with the sintering time 6 and 12 hours on the socket incisor of the left mandible. On the 7th and 14th days, the mandibular bone was cut transversely and stained. Observation of the number of fibroblast cells use 400x magnification with 3 visual fields and the mean was calculated. Data were analyzed using the Kruskal-Wallis test. Results: The mean number of fibroblast cells in groups K1, K2, P1a, P1b, P2a and P2b respectively is 23.50; 30,16; 28.94; 34.22; 30,16; and 37.50. There were significant differences in all groups, except K2-P1a, K2-P2a, and P1a-P2a. Conclusion: Calcium compounds bone graft from the synthesis of Anadara granosa with sintering time of 6 and 12 hours affect the proliferation of fibroblast cells in socket healing. Bone graft with a sintering time of 12 hours can increase fibroblast cell proliferation more effectively.

The effect of effervescent acanthus ilicifolius l. extract on antifungal properties and flexural strength acrylic Widyasri Prananingrum; Fitria Rahmitasari; Dwi Andriani; Arifzan Razak
Odonto : Dental Journal Vol 9, No 2 (2022): December 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.9.2.310-318

Background: Acanthus ilicifolius L. has antifungal potential and makes it possible to be a candidate for denture cleanser. This study aims to evaluate the effect of effervescent Acanthus ilicifolius L. extract on antifungal properties and flexural strength acrylic after immersion for three duration times.Method: Extracts were made by maceration method with ethanol. For the antifungal test, effervescent Acanthus ilicifolius L extract was prepared by combining the Acanthus ilicifolius L extract 4% and sodium perborate with two ratios, 1:7 and 1:14. The microdilution method was carried out. Effervescent Acanthus ilicifolius L extract 4% with ratio 1:7 was prepared for the flexural strength test. The fifty-four heat-cured acrylic resin (20x10x2.5mm) were fabricated and divided into 3 groups. Group 1 was immersed in aquadest (control); Group 2 was immersed in sodium perborate; Group 3 was immersed in effervescent Acanthous ilicifolius L. extract. Each group was immersed for 15 min, 8, and 56 hours (n=6). The flexural strength test was performed using Universal Testing Machine.Result: Effervescent Acanthus ilicifolius L. extract exhibited antifungal activity which increased in an effervescent Acanthus ilicifolius L extract-dependent manner significantly (0.57 to 0.50 OD). There were no significant differences between sodium perborate, effervescent_AI1 (1:7) and effervescent_AI2 (1:14). The flexural strength was found to be within the range of 66.1 - 68.3 MPa. There were no significant differences between groups (p < 0.05).Conclusion: Effervescent Acanthus ilicifolius L extract with the ratios 1:7 had a good antifungal effect and appropriate flexural strength after immersion for 15 min, 8, and 56 hours.

The effect of effervescent acanthus ilicifolius l. extract on antifungal properties and flexural strength acrylic Widyasri Prananingrum; Fitria Rahmitasari; Dwi Andriani; Arifzan Razak
Odonto : Dental Journal Vol 9, No 2 (2022): December 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Acanthus ilicifolius L. has antifungal potential and makes it possible to be a candidate for denture cleanser. This study aims to evaluate the effect of effervescent Acanthus ilicifolius L. extract on antifungal properties and flexural strength acrylic after immersion for three duration times.Method: Extracts were made by maceration method with ethanol. For the antifungal test, effervescent Acanthus ilicifolius L extract was prepared by combining the Acanthus ilicifolius L extract 4% and sodium perborate with two ratios, 1:7 and 1:14. The microdilution method was carried out. Effervescent Acanthus ilicifolius L extract 4% with ratio 1:7 was prepared for the flexural strength test. The fifty-four heat-cured acrylic resin (20x10x2.5mm) were fabricated and divided into 3 groups. Group 1 was immersed in aquadest (control); Group 2 was immersed in sodium perborate; Group 3 was immersed in effervescent Acanthous ilicifolius L. extract. Each group was immersed for 15 min, 8, and 56 hours (n=6). The flexural strength test was performed using Universal Testing Machine.Result: Effervescent Acanthus ilicifolius L. extract exhibited antifungal activity which increased in an effervescent Acanthus ilicifolius L extract-dependent manner significantly (0.57 to 0.50 OD). There were no significant differences between sodium perborate, effervescent_AI1 (1:7) and effervescent_AI2 (1:14). The flexural strength was found to be within the range of 66.1 - 68.3 MPa. There were no significant differences between groups (p < 0.05).Conclusion: Effervescent Acanthus ilicifolius L extract with the ratios 1:7 had a good antifungal effect and appropriate flexural strength after immersion for 15 min, 8, and 56 hours.

Penaeus monodon Shell Powder Extract as A Candidate for Dental Material Restoration Moh Basroni Rizal; Widyasri Prananingrum; Dian Widya Damaiyanti
Insisiva Dental Journal: Majalah Kedokteran Gigi Insisiva Vol 12, No 1 (2023): May
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/di.v12i1.16299

The prevalence of dental caries in Indonesia is still high. One of the dental caries treatments is direct restoration. Biomaterial, often used as a restorative material, is glass ionomer cement which has a silica content of 35%. Silica is found in Penaeus monodon shells which have been a waste of production. This research aims to study the characteristic of Penaeus monodon shell powder extracts as candidates for dental materials restoration. The research sample used was Penaeus monodon shell powder extract which was then carried out by a depigmentation process by mixing acetone 1:10 (w/v) ≤ 20 hours. Samples were tested with the XRD test (x-ray diffraction). SEM assessment was performed to evaluate the morphology of the particle. Data were analyzed using Mann-Whitney. The result demonstrated a significant silica content in Penaeus monodon shell powder extract before and after the depigmentation process with a p-value of 0,00 (p0,05). The depigmentation process can decrease silica content in Penaeus monodon shell powder extract. There was a different comparison of silica content in Penaeus monodon shell powder extract before and after depigmentation as a candidate for dental materials restoration.

SCHOOL HEALTH PROGRAM AS AN EDUCATIONAL FACILITY IN INCREASING KNOWLEDGE ABOUT DENTAL AND ORAL HEALTH AT BUNGA BANGSA ELEMENTARY SCHOOL Amelia Elizabeth Pranoto; Yessy Andriani Fauziah; Pambudi Rahardjo; Lita Agustia; Paulus Budi Teguh; Budi Santoso; Sularsih Sularsih; Widyasri Prananingrum; Dyah Ayu R; Nur Tsurayya; Kharinna Widowati; Yongki Hadinata
Abdi Dosen : Jurnal Pengabdian Pada Masyarakat Vol. 7 No. 3 (2023): SEPTEMBER
Publisher : LPPM Univ. Ibn Khaldun Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32832/abdidos.v7i3.1700

Dental and oral health in Indonesia is still a health problem that requires attention. To address dental and oral health problems in Indonesia, particularly in school-age and adult groups, it is necessary to carry out promotive-preventive activities by inviting local universities and business entities that focus on dental and oral health. The most common dental and oral diseases are caries and periodontal disease. One of the strategies of the Ministry of Health of the Republic of Indonesia in reducing the number of dental caries sufferers is by declaring the "Carry Free Indonesia 2030" program. The lack of knowledge and awareness of the Indonesian population regarding dental hygiene and the difficulty of reaching information due to the geographical conditions in several regions in Indonesia which make it impossible to hamper the distribution of education. The Training of Trainer Program targets teachers and parents for 21 days of morning and evening toothbrushes, teaching children and adults about the benefits of good oral hygiene and showing them how to brush their teeth twice a day with fluoride toothpaste. This program aims to provide comprehensive, applicable and sustainable education and knowledge about dental and oral health to school-age children in Indonesia.

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