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Mukarlina, Mukarlina
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Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Energy Immunology & microbiology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Social Sciences

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INVENTARISASI JAMUR MAKROSKOPIS PADA HABITAT RAWA GAMBUT Di KAWASAN CABANG PANTI TAMAN NASIONAL GUNUNG PALUNG KALIMANTAN BARAT Prayogo, Ogi; Rahmawati, Rahmawati; Mukarlina, Mukarlina
Jurnal Protobiont Vol 8, No 3 (2019)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v8i3.36841

Abstract

This inventory needs to be carried out to determine the types of macroscopic fungi that are found in the Peat Swamp Habitat of the Cabang Panti Gunung Palung National Park. Taking mushroom samples using the Purposive sampling method and roaming method by tracing the path. Identification is carried out based on macroscopic characters. Based on the results of the inventory at the Cabang Panti of Gunung Palung National Park, there were 3 macroscopic fungi from the Order of Agaricales, Auriculariales and Polyporales which which has three species including Auricularia auricula, auricularia delicata, Lichenomphalia sp. and Microporus xanthopus.
KARAKTERISASI Bacillus spp. DAN Fusarium sp. DARI TANAMAN LADA (Piper nigrum L.) DI DESA JAGA Flori, Florianus; Mukarlina, Mukarlina; Rahmawati, Rahmawati
Jurnal Protobiont Vol 9, No 1 (2020)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v9i1.40569

Abstract

Pepper (Piper nigrum L.) is one of the farm crop which become commodities in West Kalimantan. This research aimed to determine the morphological characteristic of Bacillus bacteria from rhizosphere of pepper and Fusarium fungi with symptoms of yellow disease. Sampling was done in pepper farm crops, Sungai Jaga Village, Bengkayang Regency. Isolation and identification were implemented at Microbiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Science, Tanjungpura University, Pontianak. The result of isolation and identification showed 4 bacterial isolates belong to genus Bacillus, namely Bacillus sp. BRF1, Bacillus sp. BRF2, Bacillus sp. BRF3 and Bacillus sp. BRF4 and one isolate of Fusarium sp. JDF from pepper with symptom of yellow desease.
KARAKTERISASI DAN IDENTIFIKASI GENUS BAKTERI ENDOFIT DARI DAUN DAN BATANG JERUK SIAM (Citrus nobilis var. microcarpa) SEHAT DI DESA ANJUNGAN KALIMANTAN BARAT Silalahi, Lusiana Fitriani; Mukarlina, Mukarlina; Rahmawati, Rahmawati
Jurnal Protobiont Vol 9, No 1 (2020)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v9i1.40064

Abstract

Endophytic bacteria that live in the tissues of plant organs act as biological control agents and growth accelerator such as for plants Siam Orange (Citrus nobilis var. microcarpa) which is included in main commodity horticultural crops in West Kalimantan. This study was aimed to know the genera of endophytic bacteria from healthy Siam Orange stems and leaves. The study was conducted through the steps of endophyte bacteria isolation, and identification. Systematic sampling method was used and data collected were descriptively analyzed. The study results obtained nine isolates from leaves of Bacillus, Micrococcus, Pseudomonas, Erwinia, Microbacterium, Cellulomonas and one unidentified isolate, one isolate from stems of Micrococcus.
PENGARUH KOTORAN AYAM DAN MIKORIZA Glomus aggregatum TERHADAP PERTUMBUHAN KEDELAI (Glycine max) PADA TANAH GAMBUT Iqbal, Muhamad; Linda, Riza; Mukarlina, Mukarlina
Jurnal Protobiont Vol 9, No 1 (2020)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v9i1.40621

Abstract

Peat soil in West Kalimantan has been contained low of nutrient level so it affects the growth of soybeans. One effort to increase the availability of nutrients in peat soils is by using mycorrhiza and chicken manure fertilizer. This research aims to determine the growth of soybeans by administering chicken manure and mycorrhiza on peat soils. The research was conducted from January to April 2019. The research design used was a Completely Randomized Design Factorial consisting of 2 factors with 4 replications. The first factor is mycorrhiza fungi: M1 (0 gr/polybag), M2 (5 gr/polybag), M3 (10 gr/polybag), M4 (15 gr/polybag) and the second factor is chicken manure fertilizer: P1 (0 tons/ha) , P2 (5 tons/ha), P3 (10 tons/ha), P4 (15 tons/ha). The results showed that the single chicken manure application significantly affected all observed parameters with 15 tons/ha chicken manure fertilizer. Mycorrhiza fungi just a significantly effect on fresh weight and dry weight. Also, the combination of mycorrhiza fungi and chicken manure fertilizer just a significantly affected wet weight and dry weight with combination 5 g mycorrhiza and 5 tons/ha of chicken manure fertilizer.
AKTIVITAS ANTIFUNGI EKSTRAK METANOL DAUN AKASIA (Acacia mangium Willd.) TERHADAP Phytophthora sp. (Im5) SECARA IN VITRO Wulandari, Yanti; Rahmawati, Rahmawati; Mukarlina, Mukarlina
Jurnal Protobiont Vol 9, No 3 (2020)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v9i3.46101

Abstract

Phytophthora sp. are pathogenic fungi that can cause plant diseases with symptomatic rotten or known as stem base rotten disease (BPB). Acacia leaf (Acacia mangium Willd.) has secondary metabolite content so that it can be used as a source of natural antifungal substitutes for synthetic antifungal This study uses a completely randomized design (CRD) with 4 treatment levels consisting of control, extract concentration of 2 %, 3 %, and 4 %. Antifungal activity test uses food poisoning method by adding acacia leaf extract to PDA media. The results showed that the extract of acacia leaves (Acacia mangium Willd.) had an effect on inhibiting the growth of isolate Phytophthora sp. (Im5) with the decrease of fungi colony diameter. Concentration of 4 % extract is the best concentration with the very strong level of antifungal activity in inhibiting the growth of Phytophthora sp. (Im5).
ISOLASI DAN IDENTIFIKASI JAMUR RIZOSFER DARI TANAMAN DURIAN (Durio zibethinus Murr.) DI DESA BEMBAN, KECAMATAN SUNGAI KAKAP, PONTIANAK Simangunsong, Ristamora; Rahmawati, Rahmawati; Mukarlina, Mukarlina
Jurnal Protobiont Vol 8, No 3 (2019)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v8i3.36817

Abstract

Rhizosphere fungi play an important role in the process of plant growth and development. This fungi benefits the host plant in increasing it’s growth rate, resistance to pests, diseases and drought. This study aims to determine the genus members of the durian plant rhizosphere (Durio zibethinus Murr.). Sampling of rhizosphere was carried out at Durian Plantation in Bemban Village Sungai Kakap District, Pontianak. Isolation and identification were carried out in the Microbiology Laboratory, Faculty of Mathematics and Natural Sciences, Tanjungpura University in May 2018 until December 2018. The isolation results obtained 8 rhizosphere fungi isolates.There are member of genera Aureobasidium (JRSP1), Monascus (JRSP2), Trichoderma ( JRSP3), Aspergillus (JRS4), Penicillium (JRSP5), Rhizopus (JRSP6), Aspergillus (JRSP7), Penicillium (JRSP8).
PENGARUH CEKAMAN SALINITAS GARAM NaCl TERHADAP PERTUMBUHAN KACANG TUNGGAK (Vigna unguiculata L. Walp) PADA TANAH GAMBUT Junandi, Junandi; Mukarlina, Mukarlina; Linda, Riza
Jurnal Protobiont Vol 8, No 3 (2019)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v8i3.36869

Abstract

Vigna unguiculata L. Walp is one of the cultivated plants in West Borneo, usually cultivated on dry land in the dry season and can live on land that is affected by sea water intrusion. High salt levels in the soil can cause crop growth disorders. The purpose of this study was to determine the growth response of cowpea (Vigna unguiculata L. Walp) to the stress of salinity of NaCl. The study was carried out from 2019 to June 2019. Research was carried out using a Completely Randomized Design (CRD), which consisted of four treatments namely 0 ppm, 2500 ppm, 5000 ppm and 7500 ppm. The results showed a significant effect on the parameters of plant height, root length, number of leaves and wet weight, but no significant effect on the parameters of dry weight, root nodules and effective nodules.
PENGARUH CEKAMAN AIR DAN PEMBERIAN PUPUK DAUN TERHADAP PERTUMBUHAN TANAMAN SAWI (Brassica juncea L.) Nurjanaty, Netty; Linda, Riza; Mukarlina, Mukarlina
Jurnal Protobiont Vol 8, No 3 (2019)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v8i3.36700

Abstract

The availability of water for plants will affect the process of plant growth. Water stress in plants can be overcome by applying fertilizer. Fertilizing through leaves has the advantage of absorbing nutrients needed to speed up the growth process. The purpose of this research was to use a completely randomized design (CRD) with two factors, namely the A factor of water supply consisting of 100% field capacity (KL) (A1), 75% KL (A2), 125% KL (A3), B concentration factor leaf fertilizer consists of 0 g/L (B1), 1,5 g/L (B2), 2 g/L (B3), 2,5 g / L (B4), and 3 g/L (B5) so that there is 15 combinations of treatments with 5 replications. Based on the results of research on the stress conditions of 75%, 100%, and 125% KL the best leaf fertilizer for growth of mustard green plants which includes leaf area, amount of chlorophyll, number of leaves, wet weight, dry weight, and root-shoot ratio is as much as 3 g/L.
NILAI MPN (Most Probable Number) DAN DETEKSI BAKTERI Esherichia PADA MINUMAN ES JERUK PERAS di KOTA PONTIANAK Ratna, Ratna; Rahmawati, Rahmawati; Mukarlina, Mukarlina
Jurnal Protobiont Vol 8, No 3 (2019)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v8i3.36857

Abstract

Iced squeezed orange drinks is one one of processed drinks that made from orange. Good drinking water should not contain pathogenic bacteria or organisms that may harm human health. Coliform is one ofbacteria that may cause disease in humans and being used as indicator bacteria of pathogenic bacteria. This research was aimed to know the MPN value number of coliform bacteria and to identify the bacterial presence of genus Escherichia in iced squeezed orange drinks sold in Pontianak City. The sampling used random sampling method. MPN method was used in order to know the number of coliform in iced squeezed orange drinks in Pontianak City. MPN test result obtained the lowest index value of MPN/mL was 0,14/mL in sample in subdistrict Pontianak City and the highest index value in four subdistricts in Pontianak City was MPN/mL >24,00/mL. It was showed that five samples exceed normal limits in accordance with SNI standards SNI 01-3839-1995 based on Permenkes No 492/MENKES/per/IV/2010. Identification resultshowed the bacterial genus of coliform obtained was member of genus Escherichia.
INVENTARISASI JAMUR MAKROSKOPIS FILUM ASCOMYCOTA DI KAWASAN UNIVERSITAS TANJUNGPURA PONTIANAK KALIMANTAN BARAT Kristin, Repita; Rahmawati, Rahmawati; Mukarlina, Mukarlina
Jurnal Protobiont Vol 9, No 1 (2020)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v9i1.40555

Abstract

Ascomycota is a microscopic fungi and a fraction of macroscopic, unicellular and multicellular fungi. The research of Ascomycota phylum macroscopic fungi inventory research aims to determine the type macroscopic fungi of Ascomycota phylum members in Tanjungpura University Area, Pontianak, West Borneo. The sample was taken by using Cruise Method and identified by matching morphological characteristics of the specimens with the identification book. Ascomycota  identification of macroscopic fungi samples of Ascomycota phylum based on morphological characteristics with the identification books. The results study found have 3 macroscopic fungi species consisting of 2 classes, 2 orders, 2 families, and 2 genera. In addition, Ascomycota found in Tanjungpura University Area, Pontianak, West Borneo, grow mostly on the substrate originated from dead trees.
Co-Authors Abd. Hamid Adellya Nabilla Putrie Afriza, Hanafi Agus Rachmat Antoni Antoni Ari Hepi Yanti Bahari, Anggi Dwi Diah Wulandari Rousdy Elvi Rusmiyanto Pancaning Wardoyo, Suci Lestari, Mukarlina, Febia, Ayuni Firman Saputra Flori, Florianus Iliana, Iliana Indriani, Ade Irwan Lovadi Junandi, Junandi Junardi Junardi Kristin, Repita Kustiati Kustiati Larasaty, Suprih LATIFFAH ZAKARIA Lestari, Lulu Dwi Mela, Mela Muhamad Iqbal Nova Nova Nur Asiah Nurjanaty, Netty Nurul Huda pardosi, immanuel kristian Parwati, Heni Prayogo, Ogi Rafdinal Rafdinal Rahmawati, Rahmawati Ratna Ratna Rikhsan Kurniatuhadi, Rikhsan Riyandi Riyandi Riza Linda Saputra, Firman Silalahi, Lusiana Fitriani Simangunsong, Ristamora Siti Ifadatin Siti Khotimah Siti Nur Hasanah, Siti Nur Turnip, Masnur Widasari, Rizka Wulandari, Yanti Zulfa Zakiah