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Mukarlina, Mukarlina
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Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Energy Immunology & microbiology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Social Sciences

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AKTIVITAS ANTIFUNGI EKSTRAK METANOL DAUN BANDOTAN (Ageratum conyzoides) TERHADAP Phytophthora sp. (Im5) SECARA IN-VITRO Febia, Ayuni; Mukarlina, Mukarlina; Rahmawati, Rahmawati
Jurnal Protobiont Vol 9, No 2 (2020)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v9i2.45564

Abstract

AbstractPhytophthora sp. is a fungus that causes stem rot disease in citrus plants. One of the plants that has antifungal is the leaves of Bandotan (Ageratum conyzoides). This study aims to determine the concentration of methanol extract of Bandotan leaves (A. conyzoides) which is good in inhibiting the growth of isolates of the species Phytophthora sp. (Im5). This study uses a completely randomized design (CRD) with a treatment level consisting of concentrations of 1.5%, 2%, 2.5%, 3% and control. Test for antifungal activity using the poisoning food method. The results showed a concentration of 3% Bandotan leaves methanol extract was the greatest concentration of antifungal activity inhibiting the growth of Phytophthora sp. (Im5).
PERTUMBUHAN STEK PUCUK JABON PUTIH (Anthocephalus cadamba (Roxb.) Miq.) DENGAN PERENDAMAN MENGGUNAKAN EKSTRAK KECAMBAH KACANG HIJAU (Vigna radiata) Huda, Nurul; Mukarlina, Mukarlina; Wardoyo, Elvi Rusmiyanto Pancaning
Jurnal Protobiont Vol 8, No 3 (2019)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v8i3.36730

Abstract

White jabon (Anthocephalus cadamba (Roxb.) Miq) are plants with high economic value. It can be propagated vegetatively using its shoot cuttings. One of the way to enhance the growth of shoot cuttings by submersion in green bean sprout extract was expected to produce shoot cuttings with better growth. This study aimed to determine the submersion effect of white jabon (A. cadamba) shoot cuttings in green bean (Vigna sinensis) sprout extract. The Completely Randomized Design (CRD) consisted of 5 levels of treatment were used. The cuttings were submerged in extract concentrations of 0%, 25%, 50%, 75%, and 100%. The study parameters of number of roots and leaves, plant height, wet and dry weight were observed. The results showed that the concentration treatment with 75% green bean sprout extract produced 9,25±2,50 root strands, 5,75±0,50 leaves, plant height of 20,13±1,11 cm, wet weight of 12,39±3,62 g, and dry weight of 1,51±0,42 g.
DETEKSI Liberibacter spp. PADA JERUK SIAM BERGEJALA KLOROSIS DISERTAI BERCAK HITAM DENGAN Polymerase Chain Reaction Iliana, Iliana; Rahmawati, Rahmawati; Rachmat, Agus; Mukarlina, Mukarlina; Zakaria, Latiffah
Jurnal Protobiont Vol 9, No 1 (2020)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v9i1.42703

Abstract

Liberibacter bacteria are one of the pathogenic bacteria in citrus plants. Siam Citrus Plant in Setapok Village, Singkawang City, suspected to be infected with Liberibacter spp. bacteria by showing patterns of symptoms of chlorosis accompanied by patches. This study aims to detect Liberibacter spp. on Siam Citrus leaves with symptomatic chlorosis with patches. Leaf sampling uses the roaming method in one of the citrus plantations in Setapok Village, Singkawang City. Detection of Liberibacter spp. done by PCR (Polymerase Chain Reaction) technique. Detection of bacterial DNA through several stages namely, isolation of bacterial plasmid DNA, 16S rDNA gene amplification with a thermocycler machine, and visualization using a set of electrophoresis devices. Electrophoresis results show the presence of DNA band lines, with a size of around 1100 bp for OI1 and OI2c primers, and DNA bands of 703 bp for A2 and J5 specifics. The results showed that there were Liberibacter spp. in the symptomatic chlorosis of Siam Citrus leaves accompanied by patches.
PENGARUH TUMBUHAN KABOMBA (Cabomba aquatica Aubl.) TERHADAP KADAR TSS, DO dan COD LIMBAH CAIR LABORATORIUM KIMIA FMIPA UNTAN Afriza, Hanafi; Mukarlina, Mukarlina; Rousdy, Diah Wulandari
Jurnal Protobiont Vol 8, No 3 (2019)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v8i3.36721

Abstract

Laboratory is one of the producers of waste water with a high content of hazardous materials before being discharged into water bodies. One effort to process laboratory waste with phytoremediation is to use Kabomba plants (Cabomba aquatica Aubl). This study aims to determine the ability of Kabomba (Cabomba aquatica Aubl.) To see the influence of plants on the levels of Total Suspended Solid (TSS), Dissolved Oksigen (DO) and Chemical Oxygen Demand (COD) from the Untreated Chemistry Laboratory Liquid Waste of FMIPA. This study used a Completely Randomized Design consisting of five treatments of waste concentration: control, 25, 50, 75, 100%. Based on the results of the study, Cabomba plants (Cabomba aquatica, Aubl.) able to reduce TSS values by 37.67% and increase DO values by a percentage of 77.5% on day 5 of treatment but not yet able to reduce COD values and increase pH values.
PERTUMBUHAN KEDELAI (Glycine max [L.] Merrill) VARIETAS ANJASMORO DENGAN PEMBERIAN BIOURIN KAMBING (Capra aegagrus hircus) Hamid, Abd.; Linda, Riza; Mukarlina, Mukarlina
Jurnal Protobiont Vol 9, No 1 (2020)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v9i1.40677

Abstract

Soybean (Glycine max [L.] Merrill) Anjasmoro variety is one of the superior soybean varieties widely cultivated in West Kalimantan. The growth of soybean can be increased by applying liquid organic fertilizer namely goat biourine. The purpose of this study was to determine the goat biourine quality and the effect of the application of goat biourine on the growth of Anjasmoro variety soybean plants. This study uses a Completely Randomized Design (CRD) consisting of five treatments, namely without biourine/control, 100 mL/L biourine, 200 mL/L biourine, 300 biourine mL/L and  400 mL/L biourine. Each treatment was repeated 5 times to obtain 25 experimental units. The analysis of goat biourine showed the value of C-Organic 2.03%, pH 4.83, N 0.72%, P 0.004% and K 0.234%. The results showed that apply of goat biourine had a significant effect on plant height, number of leaves, shoot wet of weight, shoot dry of weight, number of branches, number of root nodules and the number of effective root nodules, but it had not a significant effect on root wet of weight and root dry of weight.
INVENTARISASI JENIS ANGGREK (ORCHIDACEAE) TERESTRIAL DI TAMAN WISATA ALAM BUKIT KELAM KABUPATEN SINTANG Lestari, Lulu Dwi; Rafdinal, Rafdinal; Mukarlina, Mukarlina
Jurnal Protobiont Vol 8, No 3 (2019)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v8i3.36834

Abstract

Bukit Kelam Nature Tourism Park in Sintang District is a nature conservation area that has become the habitat for a wide range of endemic flora in West Kalimantan. This study aimed to identify terrrestrial orchids (Orchidaceae) species in Bukit Kelam Nature Tourism Park in Sintang District. This study was conducted from September to November 2017. The inventory of orchids specieswas done by the cruise method. The study obtained 12 terrestrial orchid species i.e Arundina graminifolia, Bromheadia finlaysoniana, Corybas pictus, Coelogyne kelamensis, Dendrobium lobii, Dendrobium olivaceum, Dipodium pictum, Liparis sp., Paphiopedilum bullenianum, Plocoglottis lowii, Spathoglottis kimbaliana,and Thrixspermumamplexicaule.
Peningkatan Kepedulian Masyarakat Kecamatan Pontianak Timur Terhadap Kesehatan Melalui Media Leaflet Rousdy, Diah Wulandari; Linda, Riza; Kustiati, Kustiati; Yanti, Ari Hepi; Rahmawati, Rahmawati; Riyandi, Riyandi; Junardi, Junardi; Ifadatin, Siti; Rusmiyanto, Elvi; Setyawati, Tri Rima; Kurniatuhadi, Rikhsan; Rafdinal, Rafdinal; Turnip, Masnur; Zakiah, Zulfa; Mukarlina, Mukarlina; Lovadi, Irwan; Saputra, Firman
Jurnal Pengabdi Vol 4, No 1 (2021): April 2021
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/jplp2km.v4i1.44379

Abstract

Kesehatan merupakan faktor kebutuhan utama manusia untuk dapat melangsungkan aktivitas hidup dengan sempurna. Kesehatan masyarakat terganggu bila terdapat kuman yang menginfeksi tubuh. Corona  atau Severe  Acute  Respiratory  Syndrome Coronavirus  2 (SARS- CoV-2) adalah virus yang menyerang  sistem pernapasan. Penyakit ini dapat menyebabkan infeksi paru-paru yang berat, hingga kematian. Data bulan April 2020, Kecamatan Pontianak Timur dilaporkan mempunyai angka kasus positif Covid-19 paling tinggi diantara kecamatan lain di Kota Pontianak.  Kelurahan di Kecamatan Pontianak Timur yang dilaporkan mempunyai angka kasus Covid-19 antara lain Kelurahan Tanjung Hulu, Kelurahan Parit Mayor, Kelurahan Banjar Serasan, Kelurahan Tambelan Sampit, Kelurahan Dalam Bugis dan Kelurahan Tanjung Hilir. Kurangnya kesadaran masyarakat tentang kesehatan dan cara mengatasi permasalahan kesehatan sangat perlu dilakukan  suatu bentuk sosialisasi dengan cara memberikan edukasi tentang permasalahan kesehatan dan cara mengatasinya. Cara yang sangat memungkinkan pada masa pandemi ini adalah dengan tidak langsung bertatap muka namun dapat dilakukan secara tidak langsung yaitu melalui leaflet dan contoh produk.  Tujuan dari kegiatan ini adalah memberikan edukasi kesehatan dalam mencegah Covid-19 kepada masyarakat. Metode yang dilakukan dalam kegiatan ini adalah memberikan edukasi dan penyuluhan melalui leaflet yaitu cara pembuatan hand sanitizer dan masker serta contoh  produk hand sanitizer dan masker yang diberikan kepada masyarakat. Sebelum pelaksanaan kegiatan, sebagian besar masyarakat (90%) di Kecamatan Pontianak Timur belum menerapkan protokol pencegahan Covid-19 yang benar. Setelah pelaksanaan kegiatan diperoleh hasil berupa peningkatan kesadaran dan pengetahuan masyarakat (100%, n=100 orang) mengenai upaya pencegahan Covid-19 serta cara pembuatan masker dan hand sanitizer.
Pertumbuhan Biji Buah Naga (Hylocereus Polyrhizus) dengan Pemberian NAA dan Ekstrak Biji Jagung (Zea Mays) secara In Vitro Widasari, Rizka; Mukarlina, Mukarlina; Zakiah, Zulfa
JURNAL BIOS LOGOS Vol 11, No 1 (2021): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.11.1.2021.31340

Abstract

(Article History: Received 25 November 2020; Revised 5 January 2021; Accepted 10 February 2021) ABSTRAKBuah naga (Hylocereus polyrhizus) merupakan salah satu tanaman yang dibudidayakan di Indonesia. Perbanyakan bibit secara in vitro dengan teknologi kultur jaringan merupakan cara alternatif yang tepat untuk menyediakan bibit dalam waktu singkat dengan jumlah banyak. Penelitian ini bertujuan untuk menguji pengaruh dan konsentrasi terbaik penambahan ekstrak biji jagung dan NAA pada media tumbuh terhadap perkecambahan biji buah naga merah secara in vitro. Penelitian dilaksanakan di Laboratorium Kultur Jaringan, Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Tanjungpura Pontianak. Penelitian menggunakan Rancangan Acak Lengkap dengan 2 faktor perlakuan. Faktor pertama yaitu NAA dengan konsentrasi 0 M, 10-8M, 10-7M, dan 5x10-7M. Faktor kedua yaitu ekstrak biji jagung dengan konsentrasi 0%, 5%, 7,5%, dan 10%. Hasil penelitian menunjukkan pemberian ekstrak biji jagung tunggal, NAA tunggal dan kombinasi ekstrak biji jagung dan NAA memberikan pengaruh nyata terhadap tinggi planlet, namun tidak berpengaruh nyata terhadap persentase perkecambahan. Pemberian ekstrak biji jagung tunggal memberikan pengaruh nyata terhadap waktu berkecambah, sedangkan pemberian ekstrak biji jagung tunggal dan NAA tunggal berpengaruh nyata terhadap jumlah akar. Perlakuan  5.10-7M NAA + 7,5% ekstrak biji jagung menghasilkan pertumbuhan tinggi plantlet terbaik. Konsentrasi 7,5% ekstrak biji jagung merupakan konsentrasi terbaik waktu berkecambahan biji buah naga dan konsentrasi NAA tunggal 10-8M memberikan jumlah akar terbanyak.Kata Kunci: Pertumbuhan in vitro, Biji Buah Naga, Ekstrak Biji Jagung, NAA.                                     ABSTRAKDragon fruit (Hylocereus polyrhizus) is one of the cultivated plants in Indonesia. In vitro propagation of seeds with the addition of synthetic growth regulators or from growth regulators from organic matter in the media, is an appropriate alternative way to provide large quantities of seeds in a short time. This study aims to determine the effect and the best effect of corn seed extract and NAA on growing media on in vitro red dragon fruit seed germination. The research was conducted at the Tissue Culture Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Tanjungpura Pontianak. The study used a completely randomized design with 2 treatment factors. The first factor is NAA with a concentration of 0 M, 10-8M, 10-7M, and 5x10-7M. The second factor is corn seed extract with a concentration of 0%, 5%, 7.5%, and 10%. The results showed that the administration of single corn seed extract, single NAA and a combination of corn seed extract and NAA had a significant effect on plantlet height, but did not significantly affect the germination percentage. The administration of single corn seed extract had a significant effect on germination time, while the administration of single corn seed extract and single NAA had a significant effect on the number of roots. The 5.10-7M NAA + 7.5% corn seed extract treatment resulted in the best plantlet height growth. The concentration of 7.5% corn seed extract was the best concentration for dragon fruit seed germination time and a single NAA concentration of 10-8M gave the highest number of roots.Keywords: In Vitro Growth, Dragon Fruit Seeds, Corn Seed Extract, NAA.
Uji Antagonis Pseudomonas flourescens spp. Terhadap Isolat Bakteri Xanthomonas (SL3) dari Daun Padi Bergejala Hawar di Kabupaten Kubu Raya Larasaty, Suprih; Mukarlina, Mukarlina; Kurniatuhadi, Rikhsan
JURNAL BIOS LOGOS Vol 11, No 1 (2021): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.11.1.2021.30998

Abstract

(Article History: Received 21 October 2020; Revised 5 November 2020; Accepted 11 November 2020) ABSTRAKPenyakit hawar pada tanaman padi disebabkan oleh bakteri anggota genus Xanthomonas. Pengendalian penyakit tanaman dapat menggunakan bakteri sebagai Agen Pengendalian Hayati (APH) yang bersifat antagonis terhadap patogen. Pseudomonas flourescens merupakan APH yang memiliki kemampuan penghambatan terhadap bakteri patogen Xanthomonas. P. flourescens spp yang diisolasi dari rhizosfer berbeda memiliki kemampuan penghambatan yang berbeda. Penelitian ini bertujuan untuk menguji kemampuan daya hambat bakteri P. flourescens spp. yang berasal dari rhizosfer yang berbeda terhadap bakteri Xanthomonas yang diisolasi dari daun padi bergejala hawar di Kabupaten Kubu Raya. Penelitian ini dilakukan pada bulan Mei 2019 bertempat di Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Tanjungpura Pontianak. Pengambilan sampel dilakukan di sentra produksi padi Desa Limbung Kabupaten Kubu Raya. Uji antagonis dilakukan di Laboratorium Mikrobiologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Tanjungpura Pontianak menggunakan metode Kirby-bauer. Hasil uji antagonis menunjukkan kedua bakteri P. flourescens spp. mampu menghambat isolat bakteri genus Xanthomonas. Jenis P. flourescens spp (Sgu5) memiliki kemampuan penghambatan terbaik hingga mencapai 8,43 mm.Kata kunci: Agen pengendalian hayati; Penyakit Hawar; Pseudomonas flourescens; Xanthomonas (SL3) ABSTRACTBlight disease in rice plants is caused by bacteria belonging to the genus Xanthomonas. Plant disease control can use the Biological Control Agent (APH) which is antagonistic to pathogens.Pseudomonas flourescens is an APH that has the ability to inhibit the pathogenic bacteria Xanthomonas. Pseudomonas flourescens spp. isolated from different rhizosphere had different inhibitory abilities. This study aims to determine the inhibitory ability of the bacteria Pseudomonas flourescens spp. against bacteria isolated from blight with symptomatic rice in Kubu Raya Regency. The research was conducted in my 2019 at the Biology Department, Faculty of Mathematics and Natural Sciences, Tanjungpura University Pontianak. Sampling was carried out at the rice production center in Limbung Village, Kubu Raya Regency.The antagonist test was carried out at the Microbiology Laboratory of the Faculty of Mathematics and Natural Sciences, Tanjungpura University, Pontianak using the Kirby-bauer method. The antagonist test result showed that both Pseudomonas flourescens spp. able to inhibit bacterial isolates of the genus Xanthomonas. Pseudomonas flourescens spp. (Sgu5) has the best inhibition ability up to 8.43 mm.Keywords: Biological control agent; Blight disease; Pseudomonas flourescens; Xanthomonas (SL3)
AKTIVITAS EKSTRAK ETANOL KULIT BUAH JENGKOL (Pithecellobium jiringa (Jack) Prain.) SEBAGAI BIOHERBISIDA GULMA BAYAM DURI (Amaranthus spinosus L.) DAN RUMPUT PAITAN (Paspalum conjugatum Berg.) Hasanah, Siti Nur; Wardoyo, Elvi Rusmiyanto Pancaning; Mukarlina, Mukarlina
Jurnal Protobiont Vol 8, No 3 (2019)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v8i3.36727

Abstract

Weed is a plant that grow in undesirable places, especially in the location of cultivated plants. Alelochemical use as bioherbicide is one of the ways in weed control and is safe for the environment. The study was conducted to test the potential of P. jiringa fruit peel extract as bioherbicide on the germination and growth ofA .spinosus and P.conjugatum weeds. The study used a completely randomized design (RAL) with the concentration of extract (P) used consisting of 6 treatments, namely P1 = 0 (control), P2 = 0.1; P3 = 0.2; P4 = 0.3; P5 = 0.4; and P6 = 0.5 g / ml. each treatment was repeated 5 times. The results of the study showed that the concentration extract of 0.5 g / ml was an inverse concentration to inhibit the weed seeds germination ofA.spinosus and P. conjugatum. Good concentration extract to inhibit growth A. spinosus and P. conjugatum is 0.3 g / ml which results in inhibition of plant height, root length, wet weight and dry weight. Based on research conducted on fruit peel extract P. jiringa has the potential as a bioherbicide.
Co-Authors Abd. Hamid Adellya Nabilla Putrie Afriza, Hanafi Agus Rachmat Antoni Antoni Ari Hepi Yanti Bahari, Anggi Dwi Diah Wulandari Rousdy Elvi Rusmiyanto Pancaning Wardoyo, Suci Lestari, Mukarlina, Febia, Ayuni Firman Saputra Flori, Florianus Iliana, Iliana Indriani, Ade Irwan Lovadi Junandi, Junandi Junardi Junardi Kristin, Repita Kustiati Kustiati Larasaty, Suprih LATIFFAH ZAKARIA Lestari, Lulu Dwi Mela, Mela Muhamad Iqbal Nova Nova Nur Asiah Nurjanaty, Netty Nurul Huda pardosi, immanuel kristian Parwati, Heni Prayogo, Ogi Rafdinal Rafdinal Rahmawati, Rahmawati Ratna Ratna Rikhsan Kurniatuhadi, Rikhsan Riyandi Riyandi Riza Linda Saputra, Firman Silalahi, Lusiana Fitriani Simangunsong, Ristamora Siti Ifadatin Siti Khotimah Siti Nur Hasanah, Siti Nur Turnip, Masnur Widasari, Rizka Wulandari, Yanti Zulfa Zakiah