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Koloni Bakteri pada Hasil Pencetakan Hidrokoloid Ireversibel setelah Direndam Rebusan Rimpang Lengkuas (Alpinia galanga) (Bacterial Colonies on Irreversible Hydrocolloid Impressing Produce after Immersion in Alpinia galanga Solution) Mohamad Basofi; Dwi Warna Aju Fatmawati; FX Ady Soesetijo
Pustaka Kesehatan Vol 3 No 1 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Background: Alpinia galanga is an herbal plant that contains antibacterial substances. The content of the antibacterial substance is useful as a disinfectant on irreversible hydrocolloid impressing produce to avoid the risk of cross infection. Objective: The objective of this study was to determine the number of bacterial colonies on irreversible hydrocolloid impressing produce after immersion in Alpinia galanga solution. Methods: The study is experimental laboratory by post-test only control group design. The sample size was 24, which was divided into 4 groups (1 control group and 3 treatment groups). Each group consisted of 6 samples. Disinfection method was by immersion for 15 seconds. Results and Conclusions: The decline levels in the number of bacterial colonies on irreversible hydrocolloid impressing produce after immersion in Alpinia galanga solution were 37.19% and 49.23%. The conclusion is that there are differences in the number of bacterial colonies on irreversible hydrocolloid impressing produce after immersion in various treatments. Alpinia galanga solution can effectively decrease the number of bacterial colonies on irreversible hydrocolloid impressing produce although descriptively the mean of it is lower than alcohol 70%.   Keywords: Alpinia galanga, irreversible hydrocolloid, bacterial colonies  
Daya Antibakteri Air Rebusan Bunga Rosella (Hibiscus sabdariffa Linn) dan Daun Teh Hitam (Camellia sinensis varian Assamica) Terhadap Penurunan Jumlah Koloni Bakteri Pada Sikat Gigi (Antibacterial Activity of Rosella Flower (Hibiscus sabdariffa Linn) a Sisca Rizkia Arifianti; Dwi Warna Aju Fatmawati; Achmad Gunadi
Pustaka Kesehatan Vol 4 No 3 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Background: Rosella flower and Black Tea Leaves is an herbal plant that contains antibacterial use full for decontamination materials toothbrush to prevent cross infection. Objective: The objective of this research was to identify the number of bacterial colonies on the water immersion results and more effective between the two materials. Method: This researchis an experimental laboratory by the posttest only control group design in 9 samples were divided into 3 groups (one control groups and the 2 treatments groups). Each group consisted of 3 samples. Decontamination method was by immersion toothbrush for 10 minutes. Result and Conclusion: The decline levels in the number of bacterial colonies on the toothbrush immersion is greatest in the Rosella group, decrease in the number of bacterial colonies after the immersion Rosella solution higher than Black Tea leaves, Rosella solution more effective as a material decontamination toothbrush. Keywords: toothbrush, Hibiscus sabdariffa, Camellia sinensis, bacterial colonies
Prevalensi Indikasi Perawatan Tumpatan pada Pasien yang Berkunjung ke RSGM Universitas Jember Tahun 2015 (The Indication Prevalence of Restoration Treatments in Patients Who Attended Dental Hospital University of Jember in 2015) Ilonavia Satiti; Dwi Warna Aju Fatmawati; Sri Lestari
Pustaka Kesehatan Vol 5 No 1 (2017)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Introduction:Teeth are hard tissue that serve as the process of mastication in humans. In the event of tooth damages, they can not perform their functions optimally. Dental hard tissue damages include caries, attrition, abrasion, erosion and fracture.The damage of tooth hard tissues can change the anatomical shape of the tooth which make them fail to function properly. Restoring the damaged anatomical shape can be achieved by restoration or fillings.Objective: To know the indication of restoration treatments in patients who attended RSGM University of Jember in 2015.Methods:This research was a descriptive study with Cross Sectional Study approach. The study was conducted at RSGM University of Jember. The number of respondents were 100 which was obtained by using the Slovin formula.Result and Conclusion: The prevalence of restoration treatments indication in patient who attended RSGM University of Jember in 2015 was 89% . It was distributed based on sex was 52,8% for women and 47,2% for men, based on age groups, 21-26 year-old-group had the highest prevalence, while 33-44 year-old-group had the lowest prevalence.  Keywords: restoration treatments, prevalence
Aktivitas Antibakteri Kombinasi Vitamin C dan Amoksisilin sebagai Bahan Alternatif Intrakanal Medikamen terhadap Enterococcus faecalis secara In Vitro Julia Eka Putri Ayuningtyas; Pudji Astuti; Dwi Warna Aju Fatmawati
Pustaka Kesehatan Vol 9 No 1 (2021): Volume 9 No.1, 2021
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v9i1.19794

Abstract

Bacteria that are often found in cases of pulp necrosis and root canal treatment are Enterococcus faecalis. Elimination of microorganisms in the root canal can use intracanal medicament. The intracanal medicament can be used from antibiotics such as amoxicillin. The antibacterial power of amoxicillin can be increased by combination with vitamin C to prevent resistance. This research aimed to determine the zone of inhibition of vitamin C and amoxicillin against Enterococcus faecalis. The method used in this study is well diffusion method. Inhibition is shown through the clear zone around the wellbore at 24 hours, 48 ​​hours, 72 hours after incubation. The number of research samples is 16 consisted of 4 groups consisting of amoxicillin, vitamin C, a combination of vitamin C and amoxicillin, and 3 MIX MP. The data were tested by non-parametric statistical analysis Kruskal Wallis and Mann Whitney. The result showed that vitamin C and amoxicillin's antibacterial activity as alternative material intracanal medicament is equivalent to antibacterial 3 MIX MP.
Uji Sitotoksisitas Ekstrak Pektin Limbah Kulit Buah Kopi Robusta (Coffea canephora) pada Kultur Sel Fibroblas Pulpa Gigi Manusia (Cytotoxicity Effect of Pectin Extract from Coffea Robusta (Coffea canephora) Fruit Peels on Human Dental Pulp Fibroblasts Cel Kharishah Muslihah; Agus Sumono; Dwi Warna Aju Fatmawati
Pustaka Kesehatan Vol 6 No 1 (2018)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v6i1.7154

Abstract

Background: Coffee has a by-product (waste product) that generated from coffee processing. Coffee fruit's peels contains active compound, that is pectin. Pectin has several benefits in health, as a regeneration of hard and soft tissue that can be applied in dentistry and pectin has the potential as an anti-cancer substance. Pectin as a biomaterial must have biocompability. Biocompatibility of the material can be tested by cytotoxicity test by MTT method on human dental pulp fibroblasts cell culture. Objective: To know the toxicity of extract pectin from coffee robusta fruit peels on human dental pulp fibroblasts cell culture. Method: This research is a experimental laboratory with post-test only control group design. Culture of fibroblast cells at the well was exposed by pectin extract for 24 hours, then each well was given MTT and incubated for 4 hours. The effects of cytotoxicity were measured by calculating cell viability (%). Data were analyzed using Probit test, One-way ANOVA followed by LSD. Result: Each group has a cell viability value above 50%. Conclusion: Extract pectin from Robusta coffee fruit peel's had no cytotoxic effect on human dental pulp fibroblsts cell culture Keywords: coffe robusta fruit peels, cytotoxicity, human pulp fibroblst cell culture, pectin
Antibacterial force of the luting-type of glass ionomer cement toward Lactobacillus species and Streptococcus mutans Dwi Warna Aju Fatmawati; Ekiyantini Widyowati
Dental Journal (Majalah Kedokteran Gigi) Vol. 40 No. 3 (2007): September 2007
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.542 KB) | DOI: 10.20473/j.djmkg.v40.i3.p131-135

Abstract

Rigid restoration is attached within oral cavity using adhesive cement layer. The hardening adhesive cement fills and tights the rough tooth surface with reciprocal opposing restorations. The luting type of glass ionomer cement was mostly used in the clinic for crown cementation as well as poured restoration. We can be taken a problem how strong is antibacterial effect of the luting type of glass ionomer cement to Lactobacillus species and Streptococcus Mutans. The purpose of the research was to know the antibacterial force of the luting-type of glass ionomer cement toward Lactobacillus species and Streptococcus mutans. This research was a laboratory experiment. The samples of the research were divided into two groups, treated-group (Fuji and Shofu) and controlled-group. The numbers of samples in each group consisted of 7 pieces. Taking 0,5 ml of artificial saliva in which the sample of the luting-type of glass ionomer cement (5 mm in diameter and 2 mm thick) had been immersed and storing it into Petri dish containing warm MRSA and 0,1 ml Lactobacillus sp. using poured technique. The mixture was subsequently incubated, and the colony was counted on the observation of 1st day, 7th day, and 14th day. The data were analyzed using ANOVA and LSD. The result of the research showed that the greatest mean value of the bacterial colony presented in the controlled-group and the smallest was in the group of Shofu. Statistical analysis showed a significant difference (p < 0.05). The released fluoride from glass ionomer cement occurred in the damaging phase caused by polyacrylate that released H+ ion from carboxyl group (COOH). The fluoride influenced the growth of bacteria by decelerating the activity of gycolytic enolase enzyme. The luting-type of glass ionomer cement had antibacterial force toward Streptococcus mutans and Lactobacillus sp. The luting-type of glass ionomer cement of Shofu possessed greater antibacterial force than Fuji and controlledgroup.
The effects of topical application of red pomegranate (Punica granatum Linn) extract gel on the healing process of traumatic ulcers in Wistar rats Sri Hernawati; Yonanda Az Zikra; Dwi Warna Aju Fatmawati
Dental Journal (Majalah Kedokteran Gigi) Vol. 52 No. 2 (2019): June 2019
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (254.511 KB) | DOI: 10.20473/j.djmkg.v52.i2.p90-94

Abstract

Background: Trauma-induced ulcers constitute one of the painful abnormalities affecting the oral cavity about which numerous individuals complain. The prevalence of traumatic ulcers (15-30% of cases) is relatively high compared to other oral lesions. Unfortunately, the use of anti-inflammatory drugs classified as steroids can lead to contraindications and serious side effects. Hence, the use of natural ingredients represents an alternative treatment. One such ingredient is red pomegranate containing bioactive elements acting as anti-inflammatory, antibacterial, antimicrobial, and antioxidant agents which accelerate the healing process in traumatic ulcers. Purpose: The aim of this study was to determine the effects of the topical application of red pomegranate (Punica granatum Linn) extract on the healing process in traumatic ulcers in Wistar rats. Methods: 24 male rats, aged 2-3 months, were divided into six groups, namely; two control groups (PC: triamcinolone acetonide and NC: sterile aquades) and four treatment groups (red pomegranate extract gel at respective concentrations of 12.5%, 25%, 50% and 75%). A traumatic ulcer was subsequently created using a flame-shaped burnisher tip 2mm in diameter and heated for ±15 seconds. The diameter traumatic ulcer of each research subject was measured and observed until it healed within the remedial parameters determined by means of a UNC-15 periodontal probe which had been placed on it. The data obtained was analyzed using Kruskal-Wallis and Mann-Whitney tests. Results: Topically applied red pomegranate extract gel can reduce the width of the ulcerated area, while also accelerating traumatic ulcer healing. There was a significant difference in the healing time between the group using red pomegranate extract gel at a concentration of 75% and the positive control group using 0.1% triamcinolone acetonide with p=0.44 (p<0.05). Conclusion: Red pomegranate extract gel possesses the ability to accelerate the healing process in traumatic ulcers. The most effective concentration of red pomegranate extract gel at accelerating the healing process in traumatic ulcers is 75%.
Effects of glycerin application on the hardness of nanofilled composite immersed in tamarind soft drinks Titis Mustikaningsih Handayani; Raditya Nugroho; Lusi Hidayati; Dwi Warna Aju Fatmawati; Agus Sumono
Dental Journal (Majalah Kedokteran Gigi) Vol. 52 No. 2 (2019): June 2019
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v52.i2.p95-99

Abstract

Background: Loss of tooth structure is a consideration in the performance of restorative treatment involving nanofilled composite resins. Material polymerization factors and water absorption can affect the hardness of composite resins. Imperfect polymerization producing an oxygen inhibited layer (OIL) and causing water absorption can even compromise the hardness of nanofilled composite resins. Tamarind soft drink, on the other hand, has an acidic pH that compromises the hardness of nanofilled composite resins. Purpose: This study aimed to reveal the effects of glycerin application on the hardness of nanofilled composite resins immersed in tamarind soft drinks. Methods: The research constituted a laboratory experiment using 24 nanofilled composite resin samples with diameters of 5mm or 2mm, divided into six groups, namely: Group G, Group G AS 60, Group G AS 120, Group TG, Group TG AS 60, and Group TG AS 120. Glycerin was applied to the surfaces of three groups before curing, while the other three groups were not treated with glycerin. Finishing was subsequently conducted on all samples using a highspeed handpiece and superfine finishing bur, before they were polished with a low speed handpiece. The samples were then divided into specific groups, namely: a group with a 120-minute immersion time, a group with a 60-minute immersion time, and a group which was not immersed and maintained at a temperature of 37oC. Each sample was tested at three points using a Vickers hardness tester (VHT). Results: The results showed that the groups with glycerin had a higher hardness level than those groups. In addition, the non-immersed groups had a higher hardness level than those groups which were immersed. The one-way ANOVA test results confirmed that there was a statistically significant difference (p<0.05) between all groups. Conclusion: The application of glycerin to nanofilled composite resins immersed in tamarind soft drinks can increase their hardness levels.
Efektivitas air perasan pulpa kakao 50% dalam membersihkan smear layer pada dinding saluran akar gigi The effectiveness of 50% cocoa pulp juice on removing smear layer of root canal dentin Disya Dwi Maulidiyah; Sri Lestari; Raditya Nugroho; Supriyadi Supriyadi; Dwi Warna Aju Fatmawati
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 33, No 3 (2021): Desember 2021
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v33i3.34435

Abstract

Pendahuluan: Smear layer merupakan lapisan tipis berisi substansi organik dan anorganik yang keberadaannya dapat menghalangi penetrasi medikamen ke tubuli dentin, sehingga harus dihilangkan dengan bahan irigasi saluran akar yaitu EDTA 17%. Bahan alami yang dapat digunakan sebagai alternatif dalam membersihkan smear layer pada dinding saluran akar adalah air perasan pulpa kakao 50% karena mengandung berbagai senyawa asam dan saponin. Tujuan penelitian menganalisis kemampuan air perasan pulpa kakao (Theobroma cacao L.) 50% dalam membersihkan smear layer pada dinding saluran akar Metode: Penelitian ini merupakan penelitian experimental laboratoris dengan rancangan penelitian the posttest only control group design. Terdapat dua kelompok uji yaitu air perasan pulpa kakao 50% dan EDTA 17%, masing-masing adalah 4 buah. Sampel yang digunakan adalah gigi insisif sapi yang direndam dalam larutan salin. Sampel dipotong mahkotanya setinggi servikal gigi dan di preparasi saluran akarnya dengan teknik konvensional menggunakan file no 45-80 dengan panjang kerja 21 mm, dirigasi dengan air perasan pulpa kakao 50% dan EDTA 17% tiap pergantian instrumen. Bagian yang diamati dengan Scanning Electron Microscope (SEM) 2000x adalah 1/3 tengah akar gigi bukal. Hasil pemotretan SEM dilakukan perhitungan skor menurut sistem skoring Hülsmann dan dilakukan analisis data dengan uji statistik Mann Whitney U Test. Hasil: Hasil penelitian dan analisis data didapatkan p=0,495 yang menunjukan bahwa tidak terdapat perbedaan bermakna antara keduanya. Kedua kelompok memiliki rerata skor 2 yang artinya sebagian orifis tubuli dentin terbuka dan terdapat sedikit smear layer. Simpulan: Air perasan pulpa kakao 50% mampu membersihkan smear layer saluran akar yang sebanding/setara dengan EDTA 17%.Kata kunci: air perasan pulpa kakao; bahan irigasi saluran akar; smear layerABSTRACTIntroduction: Smear layer is a thin layer containing organic and inorganic substances whose presence can prevent the penetration of the medicament into the dentinal tubules, so it must be removed with root canal irrigation material, namely 17% EDTA. Natural ingredients that can be used to clean the smear layer on the root canal wall are 50% cocoa pulp juice because it contains various acidic compounds and saponins. Therefore, the study aimed to analyze the ability of 50% cocoa (Theobroma cacao L.) pulp juice to clean the smear layer on the root canal walls. Methods: This study was an experimental laboratory study with the posttest only control group design. There were two test groups, namely 50% cocoa pulp juice and 17% EDTA, which were four pieces. The sample used was a bovine incisor immersed in a saline solution. The samples were crowned at the teeth' cervical level, and the root canals were prepared using conventional techniques using file no. 45-80 with a working length of 21 mm, irrigated with 50% cocoa pulp juice and 17% EDTA at each instrument change. The part observed with a Scanning Electron Microscope (SEM) 2000x was the middle 1/3 of the buccal tooth root. The results of the SEM observation were calculated according to the Hülsmann scoring system, and data were analyzed using the Mann Whitney U Test statistical test. Results: The results of the study and data analysis obtained p = 0.495, which indicates that there is no significant difference between the two. Both groups had a mean score of 2, meaning that most of the dentinal tubular orifices are open, and there is a thin smear layer. Conclusions: 50% cocoa pulp juice was able to clean the root canal smear layer, equivalent to 17% EDTA.Keywords: cocoa pulp juice; root canal irrigation agents; smear layer
HUBUNGAN BIOFILM STREPTOCOCCUS MUTANS TERHADAP RESIKO TERJADINYA KARIES GIGI Dwi Warna Aju Fatmawati
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 8 No 3 (2011)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

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Abstract

Bacteria in oral cavity are associated with oral health, as well as with dental caries. Cariogenic streptococci depend on a biofilm lifestyle for survival and persistence in the oral cavity. Primary factors allowed tooth caries is Streptococcus mutans colonization on tooth biofilms. Biofilm formation depend on carbohidrat diet n some enzyme that produced by Streptococcus mutans. It will enhance oral environment to be acid and causes tooth demineralization.