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Management of fruit and vegetable waste into ecoenzyme in Drien Rampak Village, West Aceh Regency Agustinur, Agustinur; Junita, Dewi; Lizmah, Sumeinika Fitria; Rizal, Chairul; Afrillah, Muhammad; Maulidia, Vina
Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Vol. 9 No. 4 (2024): November 2024
Publisher : University of Merdeka Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26905/abdimas.v9i4.13984

Abstract

The accumulation of waste in public areas of Drien Rampak Village remains an unresolved issue. The growing population has led to increased consumption and waste production. Currently, there is no waste management mechanism capable of addressing this problem effectively. The waste is predominantly household waste, such as organic fruit and vegetable residues. This activity aimed to provide training to the PKK women’s group in Drien Rampak Village on making ecoenzymes as a solution for managing organic waste. The implementation included training and hands-on assistance. The stages of the activity were as follows: coordination with partners, education on waste management, ecoenzyme-making training, fermentation process, and ecoenzyme harvesting. The activity produced 100 liters of ecoenzyme using 30 kg of fruit and vegetable residues. Based on pre-test and post-test questionnaire results, significant improvements were observed in participants' attitudes, knowledge, and skills in managing household organic waste. The highest improvement was in attitudes (16.84-point difference), followed by skills (13.11-point difference) and knowledge (5.49-point difference).
Exploration of Endophytic Bacteria from Rice Roots as a Biocontrol Agency for Leaf Blight Disease in Vitro Maulidia, Vina; Agustinur, Agustinur; Siregar, Mawaddah Putri Arisma; Weihan, Rayhan Amadius
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 4: Jurnal Pembelajaran Dan Biologi Nukleus December 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i4.8113

Abstract

Background: Endophytic bacteria live within plant tissues without causing disease symptoms and possess strong potential as biocontrol agents through the production of antimicrobial compounds such as bacillomycin, iturin, siderophores, hydrogen cyanide, chitinase, glucanase, protease, and volatile compounds like acetoin, 2,3-butanediol, and H₂S. These metabolites can inhibit leaf blight pathogens, including Xanthomonas oryzae. The prolonged use of chemical pesticides has caused pathogen resistance, environmental harm, and health risks, highlighting the need for eco-friendly biopesticides. Endophytic bacteria provide a sustainable alternative by suppressing pathogens through competition, biofilm formation, and inducing plant resistance. This study aims to explore the potential of endophytic bacteria isolated from rice roots as biocontrol agents against leaf blight pathogens in vitro. Methodology: Endophytic bacteria were isolated from healthy 40 days rice plants in Pajar Village, Darul Hikmah District, Aceh Jaya Regency Aceh Province. Isolation and purification were carried out using the streak plate technique, followed by macroscopic and microscopic characterization. Antagonistic ability was evaluated through the well diffusion method, and the effects of selected isolates on seed vigor and viability were assessed. Findings: A total of 25 isolates were successfully obtained, exhibiting macroscopic and microscopic diversity. Seven isolates inhibited pathogen growth by forming clear zones, with isolate AJY07 showing moderate inhibition (10 mm). These seven isolates (AJY04, AJY06, AJY07, AJY09, AJY11, AJY23, and AJY24) also enhanced seed vigor and viability and reduced infection during germination by 10–20% compared to controls. Contributions: The findings demonstrate the potential of selected endophytic bacteria as environmentally friendly biopesticides to support the development for sustainable rice cultivation