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All Journal Journal of Dentistry Indonesia Journal of Indonesian Dental Association Jurnal AKAL: Abdimas dan Kearifan Lokal Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal)

Widyarman, Armelia Sari

Faculty of Dentistry, Trisakti University

Author-ID : 1234603

Humanities Automotive Engineering Civil Engineering, Building, Construction & Architecture Dentistry Economics, Econometrics & Finance Health Professions Public Health Social Sciences Transportation

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Persistent Endodontics Pathogens Biofilm Inhibited by Lactobacillus reuteri Indonesian Strain Widyarman, Armelia Sari; Lazaroni, Njoo Kiky Elysia
Journal of Dentistry Indonesia Vol. 26, No. 3
Publisher : UI Scholars Hub

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Lactobacillus reuteri is known as probiotics that effective to improve oral health. Objective: To analyze the effect of L. reuteri Indonesian strain, towards Enterococcus faecalis and Candida albicans biofilm growth. Methods: This study was conducted using biofilm assay. Each E. faecalis ATCC-29212 and C. albicans ATCC-10231 were cultured in Brain Heart Infusion Broth, distributed into 96 well-microplate and incubated for 24h, 37°C, anaerobic condition for E. faecalis and aerobic condition for C. albicans. L. reuteri LC382415 (Indonesian strain) was cultured in deMann Rogosa Shar pe Broth and diluted into different concentrations (108,106, and 104CFU/mL) subsequently, each concent ration dist ributed into biofilm well. Biofilm wells without probiotic was used as negative cont rol. Biofilm mass were measured using crystal-violet dye at 490nm using microplate-spectrophotometer. Data was statistically analyzed by one-way ANOVA test, statistical significance set as p<0.05. Results: Significant reduction of biofilm growth of E. faecalis and C. albicans after treatment with L. reuteri. The ideal concentration was found at L. reuteri 108 CFU/mL with 79.2% E. faecalis biofilm reduction and L. reuteri 104CFU/mL with 62.5% C. albicans biofilm reduction compared to control ( p<0.05) set at 100%. The ANOVA test results showed that L. reuteri in all concentrations and all time periods in this study had the ability to inhibit biofilm growth of both species ( p<0.05) compared to negative control. Conclusion: L. reuteri Indonesian strain inhibit the biofilm growth of E. faecalis and C. albicans. This antibiofilm effect may be useful in preventing biofilm growth in root canal.

Effectiveness of Lentinus edodes Mushroom Extract on Eradication of Enterococcus faecalis Biofilm Anggiarini, Putri Lestari; Amin, Meiny F; Gunawan, Juanita A; Widyarman, Armelia Sari
Journal of Dentistry Indonesia Vol. 27, No. 2
Publisher : UI Scholars Hub

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Sodium hypochlorite is a commonly used irrigation solution in endodontic procedures, but it irritates tissues and has toxic effects. Lentinus edodes is a mushroom that has antibacterial properties. Enterococcus faecalis is an anaerobic bacterium that can cause root canal treatment failure. Objective: This study aimed to determine the effect of L. edodes extract on the eradication of E. faecalis biofilms. Methods: Phytochemical tests of L. edodes were performed to analyze alkaloids, steroids, triterpenoids, phenolics, tannins, flavonoids, and glycosides from this extract qualitatively. E. faecalis ATCC 29212 was cultured in brain heart infusion broth for 24 h at 37°C in an anaerobic atmosphere. Biofilm assay was performed to analyze the eradication of E. faecalis biofilm after treatment with L. edodes extract. The application times were 5, 15, and 30, and 10%, 20%, 40%, and 80% concentrations were used. Distilled water was used as a negative control, and NaOCl was used as a positive control. Data were statistically analyzed via one-way analysis of variance, where p < 0.05 was set as the level of significance. Results: L. edodes mushroom extract was effective in eradicating E. faecalis biofilms in all concentrations and incubation times compared with the control (p < 0.05). Significant differences were found between the application times of 5 and 15 min compared with 30 min (p < 0.05). The most effective concentration in eradicating E faecalis biofilms was 40% with an application time of 30 min. Conclusion: L. edodes mushroom extract proves its antibiofilm activity against E. faecalis biofilm. Further study is necessary to determine which substances are have the most influence on the effectiveness of L. edodes extract in eradicating E. faecalis biofilm in vivo.

Antibacterial and Antibiofilm Efficacy of Pineapple Hump (Ananas comosus) on Porphyromonas gingivalis in vitro Soulissa, Abdul Gani; Lombardo, Billy; Widyarman, Armelia Sari
Journal of Dentistry Indonesia Vol. 28, No. 3
Publisher : UI Scholars Hub

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Background: Periodontal disease is one of the most prevalent oral health problems in Indonesia that affects supporting tissues of the teeth. Porphyromonas gingivalis plays an important role in the pathogenesis of periodontal disease. Alternative therapy with natural plant extracts, including pineapple (Ananas comosus) hump extract may inhibit the growth of bacteria that cause periodontal disease. Objective: To determine the effect of pineapple hump extract on bacterial growth and adhesion of Porphyromonas gingivalis biofilms. Method: The bacterial inhibition test was performed by the agar well diffusion method, and biofilm density measurements were made using the biofilm assay method. Results: Pineapple hump extract can inhibit bacterial growth optimally at a concentration of 100%, with an average zone of inhibition of 7.3 mm. The extract at a 50% concentration can eradicate the biofilms in a 6 h incubation time with an average OD of 0.124. The Shapiro-Wilk method confirmed a normal distribution of the data. Both one-way ANOVA and post hoc test showed a significant difference between the inhibitory ability of pineapple hump extract and between concentrations to restrict formation of Porphyromonas gingivalis biofilms. Conclusion: Pineapple hump extract was able to inhibit bacterial growth and eradicate the adhesion of Porphyromonas gingivalis biofilms in vitro.

The Effect of Parabiotic Reuterin on the Expression of Genes Involved in Candida albicans Biofilm Formation: An Ex vivo Study Riad, Anastasya Muna; Widyarman, Armelia Sari
Journal of Dentistry Indonesia Vol. 28, No. 3
Publisher : UI Scholars Hub

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Candida albicans has a number of properties, including resistance to various antimicrobial agents, which allow it to survive in the root canals. Lactobacillus reuteri plays a role in maintaining oral health through interactions with the oral microbiome. L. reuteri has potential as a preventive and therapeutic agent against inflammatory diseases. Objective: The aim of this study was to investigate the effect of irrigation with a reuterin-containing solution on BCR1, ACE2, EFG1, and TEC1 gene expression in C. albicans root canal biofilms. Methods: L. reuteri was cultured in MRS broth and incubated anaerobically for 24 hours at 37°C. C. albicans was cultured in Sabouraud dextrose broth at 37°C for 48 hours. A total of 24 single-rooted premolar teeth were standardized and inoculated with C. albicans before irrigation with 50 μg/mL reuterin as a single, independent variable (Indonesian strain), 50 μg/mL reuterin Prodentis (a strain combination of L. reuteri DSM 17938 and L. reuteri ATCC PTA 5289), 2.5% sodium hypochlorite as positive control, and saline as negative control. A real-time quantitative polymerase chain reaction (RT-qPCR) assay was used to detect the expression of BCR1, ACE2, EFG1, and TEC1 in C. albicans root canal biofilms. Results: Reuterin significantly reduced the expression of BCR1 and ACE2 genes, which play a role in C. albicans biofilm formation, at the biofilm maturation stage (P < 0.05). Reuterin also affected the expression of the EFG1 and TEC1 genes, although the effect was not significant. Conclusion: A reuterin isolate of L. reuteri exhibits antibiofilm activity against the expression of C. albicans genes involved in biofilm formation. Reuterin has potential as an irrigation agent in the treatment of root canals. Further research is needed to shed light on the effectiveness of reuterin against the expression of genes that play important roles in the formation of C. albicans biofilms.

In vitro Antibiofilm Activity of Pomegranate (Punica granatum) Juice on Oral Pathogens Jemima Pramadita; Armelia Sari Widyarman
Journal of Indonesian Dental Association Vol 2 No 1 (2019): April
Publisher : Indonesian Dental Association

Introduction: Pomegranate (Punica granatum) fruit contains valuable ingredients, such as ellagitannins and flavonoids, that have many potential effects, including antibacterial, antifungal, and anti-inflammatory functions. Objectives: The aim of this study was to investigate the effects of pomegranate fruit juice on F. nucleatum and S. sanguinis monospecies and multispecies biofilm formation in vitro. Methods: Pomegranate juice was obtained using a juicer and diluted using a brain heart infusion (BHI) broth into five different concentrations. The biofilm assay was performed as follows: F. nucleatum and S. sanguinis were cultured separately in the BHI broth for 48 hours at 37°C in an anaerobic atmosphere. A 200 mL bacterial suspension (107 CFU/mL) was distributed into a 96-well plate and incubated for 24 hours to form a biofilm. Subsequently, pomegranate juice was added to the biofilm well and observed after 1 hours, 3 hours, 6 hours, and 24 hours. The biofilm mass was measured using a microplate reader (490 nm) after crystal violet staining. Chlorhexidine (0.2%) and the biofilms without treatment were used as the positive and negative controls, respectively. The data were statistically analyzed using one-way analysis of variance, with p<0.05 as the level of significance. Result: There was a significant biofilm reduction after treatment with pomegranate juice for all the concentrations and incubation times (p<0.05). The effective concentrations to inhibit the biofilm monospecies F. nucleatum and S. sanguinis and the multispecies were 6.25% (OD 0.148±0.019), 50% (OD 0.211±0.026), and 6.25% (OD 0.024±0.209), respectively. Conclusion: Pomegranate juice inhibits F. nucleatum and S. sanguinis biofilm formation as a monospecies and a multispecies. Future studies are needed to observe the mechanism of this active substance.

The Effect Ant-Nest Plant (Myrmecodia pendans) Extract on Streptococcus sanguinis and Treponema denticola Biofilms Nely Soviati; Armelia Sari Widyarman; Ciptadhi Tri Oka Binartha
Journal of Indonesian Dental Association Vol 3 No 1 (2020): April
Publisher : Indonesian Dental Association

Introduction: Ant-nest plant (Myrmecodia pendans) contains triterpenoid, flavonoid, saponin and tannin that acts as antibacterial substance. Objectives: This study’s aim was to analyze the effect of Myrmecodia pendans extract effect towards biofilm of Streptococcus sanguinis and Treponema denticola. Methods: Ant-nest plant was extracted using maceration technique. The obtained extract was diluted into five different concentrations using phosphate buffer saline (PBS). S. sanguinis and T. denticola ATCC 35405 were cultured in Brain Heart Infusion (BHI) broth, 48h, 37°C, anaerobic atmosphere and distributed into four 96 well-plate for 24h to form biofilm. Subsequently, the extract was distributed into the well that contained the mono-species and multispecies biofilm and then incubated for 1h, 3h, 6h and 24h. Chlorhexidine (0.2%) was used as positive control and BHI broth was used as negative control. The biofilm was stained using crystal-violet and measured by microplate reader with a wavelength of 490 nm. Data were statistically analyze using one-way ANOVA test and Post Hoc LSD test which p < 0,05 was set as significant difference. Result: The most effective concentrations to reduce mono-species biofilm were 100% in 1h incubation period for S. sanguinis (OD = 1,403 ± 0,019) and 24h for T. denticola (OD = 1,012 ± 0,037) and multispecies biofilm (OD = 0,984 ± 0,001) compared to negative control. Statistical analysis showed that ant-nest plant extract significantly reduced S. sanguinis, T. denticola and multispecies biofilm mass compared to negative control (p < 0,05). Conclusion: The ant-nest plant extract has inhibitory effects against S. sanguinis and T. denticola biofilm and it may be used as alternative for dental therapy. Future studies are needed to evaluate the potential of ant-nest plant extract in multispecies composed of other oral bacteria.

Antibiofilm Effect of Clitoria ternatea Flower Juice on Porphyromonas gingivalis in vitro Armelia Sari Widyarman; Stephani Sumadi; Tri Putriany Agustin
Journal of Indonesian Dental Association Vol 1 No 1 (2018): October
Publisher : Indonesian Dental Association

Introduction: Clitoria ternatea flower contains flavonoid such as anthocyanin that gives the blue color to its flower and has antimicrobial activity. Objectives: The aim of this study was to examine the effect of flower juice of Clitoria ternatea against Porphyromonas gingivalis biofilm viability in vitro. Methods: This study was experimental laboratory research using biofilm assay method. P. gingivalis was cultured in BHI broth in 37°C for 24h under anaerobic condition. Fresh flowers of Clitoria ternatea were extracted using mortar and pestle and diluted into 6 different concentrations: 100%, 50%, 25%, 12.5%, 6.25%, and 3.125% with phosphate buffer saline (PBS). Chlorhexidine (0.2%) was used as positive control and biofilm without treatment as negative control. The flower juice was distributed into 96 well-plates that contained biofilm of P. gingivalis and incubated for 1h, 3h, 6h, and 24h in 37°C, anaerobic atmosphere. Biofilm was measured using crystal violet dye with microplate reader (490 nm). Data were statistically analysed using one-way ANOVA test and Post Hoc test with p<0.05 was set as significant different. Result: Result showed that Clitoria ternatea flower juice significantly reduced the Porphyromonas gingivalis biofilm viability in all concentration and all incubation time. The most effective concentration to inhibit Porphyromonas gingivalis biofilm was 100% in 1h incubation time which biofilm was diminished (Optical Density=0.00). One way ANOVA test and Post Hoc test showed a significant biofilm reduction in all concentration and all incubation time after treatment with the flower juice compared to control (p<0.05). Conclusion: Clitoria ternatea flower juice has antibiofilm effect against Porphyromonas gingivalis. This result showed this flower juice may be useful for combating periodontal pathogens. However, further studies using other bacteria are still needed to confirm this result.

The Comparison of Metronidazole, Clindamycin, and Amoxicillin Againts Streptococcus sanguinis Kevin Lim; Armelia Sari Widyarman
Journal of Indonesian Dental Association Vol 1 No 1 (2018): October
Publisher : Indonesian Dental Association

Introduction: Viridans streptococci group such as Streptococcus sanguinis (S. sanguinis), an anaerobic Gram-positive bacteria is a well-known for its involvement in dry socket (alveolar osteitis)-associated infection. Systemic amoxicillin, clindamycin and metronidazole have all been shown to be effective to inhibit this bacterium. However, there has been a lack of studies identifying which are the most effective amongst these antibiotics toward Streptococcus sanguinis. Objectives: The purpose of this study is to evaluate the effectiveness of metronidazole, clindamycin, and amoxicillin in inhibiting the growth of Streptococcus sanguinis in vitro. Methods: This effectiveness was done by using agar well diffusion methods. S. sanguinis ATCC 10556 were cultured in Brain Heart Infusion (BHI) broth at 37°C under anaerobic condition. After 48h, bacterial cells were harvested and counted using microplate reader (490 nm) to achieve optical density of 0.25-0.30 (107 CFU/mL). Subsequently, 100 μL of bacterial suspension was cultured on BHI agar and each antibiotic suspension was added into each agar well, incubated for 72h at 37°C. The inhibition zone diameters were measured with electronic caliper. All experiments were done in triplicate, and repeated two times in separated occasions. The obtained data were statistically analyzed using one-way ANOVA test. A p<0.05 was considered as significance. Result: The results showed that there was a significant difference in the effectiveness, clindamycin and amoxicillin in inhibiting the growth of Streptococcus sanguinis (p<0.05), compared to metronidazole. The inhibition zone diameter with mean±SD (mm) are 13.50±2.0, 34.67±2.3 and 32.67±1.7 for metronidazole, clindamycin and amoxicillin, respectively. Conclusion: Clindamycin and amoxicillin are more effective in inhibiting the growth of Streptococcus sanguinis compared to metronidazole in this study. However, future studies are needed to confirm this result in vivo.

DETEKSI DINI GIGI TIDAK TERATUR (MALOKLUSI) PADA ANAK PERIODE GIGI CAMPUR MURID SD MUHAMMADIYAH 06 TEBET-JAKARTA SELATAN Yohana Yusra; Joko Kusnoto; Armelia Sari Widyarman; Arianne Dwimega; Febri Fahmawati; Nicolas Brian Simanjuntak; Adzra Fadla Taqia; Sasqia Faadilah Andikoputri; Faadiyah Nisa
Jurnal AKAL: Abdimas dan Kearifan Lokal Vol. 4 No. 1 (2023): Jurnal AKAL : Abdimas dan Kearifan Lokal
Publisher : Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/akal.v4i1.15841

Maloklusi merupakan masalah Kesehatan masyarakat yang dapat dicegah (Preventif) ataupun dihalangi (Interseptif). Ortodonti interseptif adalah prosedur perawatan pada periode gigi campur, untuk memperbaiki maloklusi yang mulai terjadi dan mengurangi keparahan pada periode gigi permanen. Deteksi dini terhadap perkembangan terjadinya maloklusi dan potensial prosedur perawatan ortodonti yang sederhana dapat meminimalkan atau menghilangkan perawatan ortodonti yang memerlukan biaya cukup besar. Saat masa pandemi Covid-19 seperti saat ini proses pembelajaran pada semua institusi pendidikan hampir sebagian besar masih dilakukan secara daring dan dengan adanya peraturan mengenai pembatasan kegiatan masyarakat dapat menyebabkan salah satunya adalah hilangnya kesempatan untuk melakukan pemeriksaan gigi anak secara rutin ke dokter gigi. Beberapa masalah yang ditemukan pada orang tua dan anak murid SD Muhammadiyah 06 Tebet Timur diantaranya adalah tidak adanya penyuluhan mengenai kesehatan gigi dan mulut selama masa pandemi Covid-19, dan keterbatasan orang tua untuk memeriksakan gigi anaknya secara rutin ke dokter gigi selama masa pandemi. Pengabdian kepada masyarakat telah dilakukan pada hari Sabtu tanggal 26 Maret 2022 secara daring berupa penyuluhan kesehatan gigi anak secara umum dan pelatihan deteksi dini gigi tidak teratur (Maloklusi) pada anak. Kegiatan berjalan dengan lancar dan analisis terhadap kuesioner yang diberikan untuk mengetahui prior knowledge para orang tua menunjukkan masih kurangnya pengetahuan orang tua mengenai kesehatan gigi anak dan maloklusi

Effectiveness of Matricaria chamomilla Essential Oil on Aggregatibacter actinomycetemcomitans and Treponema denticola Biofilms Nurrahman, Hana Fauziyah; Widyarman, Armelia Sari
Journal of Indonesian Dental Association Vol 3 No 2 (2020): October
Publisher : Indonesian Dental Association

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Introduction: Matricaria chamomilla (chamomile) is known to possess antimicrobial, anti-inflammatory, and antioxidant properties. Objectives: The purpose of this study was to determine how effectively Matricaria chamomilla essential oil acts against Aggregatibacter actinomycetemcomitans and Treponema denticola biofilms in vitro. Methods: Aggregatibacter actinomycetemcomitans ATCC-29522 and T. denticola ATCC-35405 were separately cultured in brain heart infusion (BHI) broth at 37°C for 2 4h in anaerobic conditions. Each bacterial suspension (200 uL, 107 CFU/mL) was cultured in 96-well plates for 48 h to form a biofilm. Thereafter, biofilms were treated with chamomile essential oil at concentrations of 3.12%, 6.25%, 12.5%, 25%, 50%, and 100% in a time-dependent experiment. Readings were taken at 1 h, 3 h, 6 h, and 24 h. Biofilm mass was evaluated using crystal violet staining (for A. actinomycetemcomitans) and safranin staining (for T. denticola). Biofilms treated with chlorhexidine (0.2%) and untreated biofilms were used as positive and negative controls, respectively. Data were statistically analyzed using one-way analysis of variance (ANOVA), with the significance level set to p<0.05. Results: Chamomile essential oil significantly reduced the biomass of the biofilms (p<0.05). The most effective chamomile oil concentrations for inhibiting A. actinomycetemcomitans and T. denticola biofilms were 100% and 50%, respectively, with 24 h incubation periods. The results of ANOVA and the post hoc Least Significant Difference (LSD) test showed a significant reduction (p<0.05) in biofilm mass for all concentrations of chamomile essential oil compared to the negative control across all incubation times. Conclusion: The data suggest that chamomile essential oil can inhibit the biofilm formation of A. actinomycetemcomitans and T. denticola biofilms. It could, therefore, be useful as an alternative treatment to inhibit the biofilms composes of the bacteria tested in periodontal disease cases. However, continued researches are necessary to further explore the mechanisms of this effect.

Co-Authors Adzra Fadla Taqia Amin, Meiny F Anastasia E Prahasti Anggiarini, Putri Lestari Arianne Dwimega Boy M Bachtiar Celia Radita, Diajeng Ciptadhi Tri Oka Binartha Dewi Liliany Margaretta, Dewi Liliany Dhara Nandary Drestia, Aradhea Monica Endang Winiati Bachtiar Erik Idrus Erni Erfan, Erni Faadiyah Nisa Febri Fahmawati Gunawan, Juanita A Hepziba, Evangelista Rachel Howis Josephine Indrayadi Gunardi, Indrayadi Janti Sudiono Jemima Pramadita Joko Kusnoto Josephine, Howis Kevin Lim Lazaroni, Njoo Kiky Elysia Lombardo, Billy Maulina, Syarifah Aulia Melanie Sadono Djamil Nely Soviati Nicolas Brian Simanjuntak Nurrahman, Hana Fauziyah Olivia Puspita Suhalim Putriany Agustin, Tri Rasad, Irvan Septrian Syah Putra Riad, Anastasya Muna Richi, Mario Roeslan, Moehamad Orliando Sasqia Faadilah Andikoputri Shirley Trisna Yunita Soesanto, Sheila Soulissa, Abdul Gani Stella Pranoto Stephani Sumadi Stephanie Brigitta Widjaja Suwartini, Tien Tanzil, Felicia Klarissa Theodorea, Citra Fragrantia Tjokro Prasetyadi Tri Erri Astoeti Tri Erri Astoeti Uppalavanna Witedja Widijanto Sudhana Widijanto Sudhana Wiwiek Poedjiastoeti, Wiwiek Yohana Yusra

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