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Journal : BIOEDUKASI

Isolation and Characterization of Sugarcane Root (Saccharum sp.) Rhizobacteria and Their Influence on the Growth of Lettuce Plants (Lactuca sativa L.) Rismaningsih, Eka; Khumaira, Annisa; Nugraheni, Ika Afifah
BIOEDUKASI Vol 22 No. 2 (2024)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v22i2.43596

Abstract

Lettuce (Lactuca sativa L.) is a horticultural commodity with good commercial value. Lettuce production is insufficient for consumer needs because of the excessive use of inorganic fertilizers, which reduce soil fertility. This research aims to decide rhizobacteria in the rhizosphere of sugarcane roots and their influence on increasing the growth of lettuce plants. The methods used are observational methods for taking composite soil samples. Macroscopic observations of bacterial colonies include the shape, elevation, edges, and color of the colonies produced. Through gram staining and physiological tests, including catalase, motility, carbohydrate fermentation, and indole tests, performed microscopic character observations. Field tests as PGPR were carried out on lettuce plants with treatment P0, P1, P2 and P3. The parameters observed were plant height, number of leaves, wet weight, dry weight, and root length. Based on the results of the research, the PGPR of sugarcane roots has rhizobacteria such as Corynebacterium sp, Terrabacter, Saccharococcus, Aeromicrobium, Paracoccus, Ancylobacter, Planococcus, and Azotobacter. PGPR affects the growth of lettuce plants, especially on plant height, number of leaves, and root length. Treatment P3 (PGPR 7.5 ml/L) and treatment P1 (2.5 ml/L) obtained the best concentrations, yielding optimal results on all test parameters. Treatments P0 and P2 did not have a significant effect on the growth of lettuce plants.
Co-Authors Ade Maulida Viantini Aditya Agus Pradana Agustianingrum, Sekar Aisyah Rahma Putri Alfa Fitri Amalia Hilal Alifianto, Luthfi Galih Alifiyah Tunnisah Mony Amalia Hilal, Alfa Fitri Annisa Khumaira Arif Bimantara Arif Yusuf Wicaksana Ata Rofita Wasiati Deccy Nopiyana Delfia Desi Erlita Dinar Mindrati Fardhani Dittasari Putriana Doddy Sulistiawan Wibowo Doddy Sulistiawan Wibowo Fathah, Rigel Nurul Fayakun Nur Rohmah Fitria Damayanti Fitriahadi, Enny Fradana Ade Saputra Fuad Gandhi Torizal Fuadiyah, Dzakiya Alda Gevy Nuradira Isnaini Heni Setianah Heni Setianah Heni Setianah Inneke Ashri Mawaddah Islamiyatur Rokhmah Khusnul Hidayah Luluk Rosida Mawaddah, Inneke Ashri Muhammad Arjun Najib Muhammad Arjun Najib Muhammad Dwi Ramdhan Nabila, Tsania Taskia Najib, Muhammad Arjun Nirmala Ayu Arifah Nita Widyaningsih Noviani, Nor Eka Nugroho, Hendrato Setiabudi Nur Hanifah Fauziyyah Oktavianti, Lyna Hidayati Puspitasari, Amallia Rais Sulistyo Widiyatmoko Rifka Amalia Rindi Astuti Rismaningsih, Eka Rohmadani, Zahro Varisna Rohmadoni, Zahro Varisna Rohmawati, Rizka Salsa Bernadetha Setianah, Heni Sharfina Mutia Syarifah Siti Maisaroh Sumarni Suri Salmiyati Suri Salmiyati, Suri Suryadi Suryadi Susila, Wisnu Adhi Tasya Muliasari Teti Anggita Safitri, Teti Anggita Tian Setiawan Tiara Sekar Ayuni Titin Aryani Tsania Taskia Nabila Viko Aldi Putra Viko Aldi Putra Wahyuni Wulansari Wahyuningsih, Hapsari Wasiati, Ata Rofita Wibowo, Doddy Sulistiawan Wicaksana, Arif Yusuf Widiyatmoko, Rais Sulistyo Wisnu Adhi Susila Wiwit Probowati Yuli Setiawati Yuli Setiawati, Yuli