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All Journal Media Teknologi Hasil Perikanan Jurnal MIPA PHARMACON JURNAL BIOMEDIK CHEMISTRY PROGRESS Briliant: Jurnal Riset dan Konseptual Jurnal Farmasi Medica (Pharmacy Medical Journal) PMJ Jurnal Abdi Insani Vivabio : Jurnal Pengabdian Multidisiplin Malacca Pharmaceutics Grimsa Journal of Science Engineering and Technology Journal of Community Empowerment Jurnal Lentera Farma
Antasionasti, Irma
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Physics Public Health Social Sciences

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AKTIVITAS ANTIOKSIDAN EKSTRAK TERIPANG (H.atra) DENGAN METODE DPPH (1,1-diphenyl-2-picrylhydrazyl) Bawole, Avinda Shania Wiane; Wewengkang, Defny S.; Antasionasti, Irma
PHARMACON Vol 10, No 2 (2021): PHARMACON
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.10.2021.34036

Abstract

ABSTRACTUtilization of marine life is not just consumptive but can be used as raw material for medicines, one of which is sea cucumber. Sea cucumbers (H.atra) are marine invertebrates belonging to the Echinoderms phylum which have been studied containing antioxidants. Therefore, a study was conducted to determine the antioxidant activity of sea cucumber (H.atra) extract taken from the waters of Manado Bay using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method. Sea cucumber samples were extracted using the maceration method. The ethanol extract of sea cucumber showed high antioxidant activity at a concentration of 8 μg / ml with 77.83% inhibition percentage. Keywords: H.atra, Antioxidant, DPPH (1,1-diphenyl-2-picrylhydrazyl).  ABSTRAKPemanfaatan biota laut bukan hanya sekedar konsumtif saja tapi dapat berpotensi sebagai bahan baku obat-obatan salah satunya teripang. Teripang (H.atra) adalah hewan invertebrata laut yang termasuk ke dalam filum Echinodermata  yang pernah diteliti mengandung antioksidan. Oleh karena itu, dilakukan penelitian untuk mengatehui aktivitas antioksidan dari ekstrak teripang (H.atra) yang diambil dari perairan Teluk Manado dengan menggunakan metode DPPH (1,1-diphenyl-2-picrylhydrazyl). Sampel teripang diekstraksi menggunakan metode maserasi. Ekstrak etanol teripang menunjukkan aktivitas antioksidan yang tinggi pada konsentrasi 8 μg/ml dengan persen inhibisi 77,83%. Kata kunci:  H.atra, Antioksidan, DPPH (1,1-diphenyl-2-picrylhydrazyl)
UJI EFEKTIVITAS SENYAWA FLAVONOID DARI EKSTRAK ETANOL DAUN SAWILANGIT (Vernonia cinerea (L.) Less) SEBAGAI ANTIPIRETIK PADA TIKUS PUTIH JANTAN GALUR WISTAR (Rattus norvegicus) YANG DIINDUKSI VAKSIN DPT Samiun, Asriyani; Queljoe, Edwin De; Antasionasti, Irma
PHARMACON Vol 9, No 4 (2020): PHARMACON
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.9.2020.31367

Abstract

ABSTRACTSawilangit leaves (Vernonia Cinerea (L.) Less) is a plant that contains flavonoids as an antipyretic. The purpose of this study was to determine the effectiveness of flavonoid compounds from ethanol extract of sawilangit (Vernonia cinerea (L.) Less) leaves as an antipyretic in male white rats Wistar strain (Rattus norvegicus) induced by DPT vaccine. A total of 15 male rats were divided into 5 treatment groups, namely the negative control group (CMC 1%), the positive control group (paracetamol), and the ethanol extract group of sawilangit leaves with a dose of 200 mg, a dose of 400 mg, a dose of 800 mg. Each mouse was induced intramuscularly with a DPT vaccine at a dose of 0.3 ml. Temperature measurements were carried out before and after induction, then every 30 minutes after giving the test material from the 30th minute to the 180th minute. Data were analyzed statistically with One Way ANOVA and continued with LSD test to see significant differences between treatments. The results showed that the ethanol extract of sawilangit leaves had an antipyretic effect on male white rats Wistar strain..Key words: Antipyretics, Vernonia Cinerea, male white rats ABSTRAKDaun Sawilangit  (Vernonia Cinerea (L.) Less) adalah salah satu tanaman yang mengandung zat Flavonoid sebagai antipiratik. Tujuan penelitian ini untuk mengetahui adanya efektivitas senyawa flavonoid dari ekstrak etanol daun sawilangit (Vernonia cinerea (L.) Less) sebagai antipiretik pada tikus putih jantan galur wistar (Rattus norvegicus) yang diinduksi vaksin DPT. Sebanyak 15 ekor tikus putih jantan dibagi 5 kelompok perlakuan yaitu kelompok kontrol negatif (CMC 1%), kelompok kontrol positif (parasetamol), dan kelompok ekstrak etanol daun sawilangit dosis 3,6 mg, dosis 7,2 mg, dosis 14,4 mg. setiap tikus diinduksi vaksin DPT dosis 0,3 ml secara intramuscular. Pengukuran suhu dilakuan sebelum dan sesudah induksi, kemudian setiap 30 menit  setelah pemberian bahan uji dari menit ke-30 sampai menit ke-180. Data dianalisis secara statistika dengan One Way ANOVA dan dilanjutkan uji LSD untuk melihat beda nyata antar perlakuan. Hasil penelitian menunjukan bahwa Ekstrak etanol daun sawilangit mempunyai efek antipiretik terhadap tikus putih jantan galur wistar. Kata kunci: Antipiretik, Vernonia Cinerea, tikus putih jantan
Penilaian Kualitas Hidup Pada Pasien Kanker di Ruangan Irina Delima RSUP Prof. Dr. R. D. Kandou Manado Maringka, Pingkan C.; Wiyono, Weny I.; Antasionasti, Irma
Jurnal Biomedik : JBM Vol 12, No 2 (2020): JURNAL BIOMEDIK : JBM
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.12.2.2020.29286

Abstract

Abstract: Cancer is known as one of the diseases that has a serious physical and psychological impact on sufferers, the presence of cancer can affect the quality of life of patients. This study aims to determine the quality of life in cancer patients at irina delima room of RSUP Prof. Dr. R. D. Kandou Manado. This study was a descriptive study with a cross-sectional approach and data collected using the EORTC QLQ C30 questionnaire to 56 cancer patients. The results of the research obtained  quality of life on global health scale categorized as good with a score of 67,3, on the functional scale is categorized as good with the highest average value of emotional function of 78,2, and the scale of symptom is categorized as poor with the highest average value of fatigue of 48,6.Keywords : Quality of life, cancer, EORTC QLQ-C30.                                                                Abstrak: Kanker dikenal sebagai salah satu penyakit yang memiliki dampak serius terhadap fisik dan psikologis bagi penderitanya, keberadaan penyakit kanker dapat mempengaruhi kualitas hidup pasien kanker. Penelitian ini bertujuan untuk mengetahui kualitas hidup pada pasien kanker di ruangan irina delima RSUP Prof. Dr. R. D. Kandou Manado. Penelitian ini merupakan jenis penelitian deskriptif dengan pendekatan cross sectional dan pengumpulan data menggunakan kuesioner EORTC QLQ-C30 terhadap 56 pasien kanker. Hasil penelitian yang diperoleh yaitu kualitas hidup pada skala kesehatan global terkategori baik dengan diperoleh nilai sebesar 67,3, pada skala fungsional terkategori baik dengan nilai rata-rata tertinggi yaitu fungsi emosi sebesar 78,2, dan pada skala gejala terkategori buruk dengan nilai rata-rata tertinggi yaitu kelelahan sebesar 48,6.Kata Kunci : Kualitas hidup, kanker, kuesioner EORTC QLQ-C30 
KARAKTERISASI NANOPARTIKEL EKSTRAK ETANOL KAYU MANIS (Cinnamomum burmanii) DENGAN KITOSAN SODIUM TRIPOLIFOSFAT SEBAGAI KANDIDAT ANTIOKSIDAN Antasionasti, Irma; Jayanto, Imam; Abdullah, Surya Sumantri; Siampa, Jainer Pasca
CHEMISTRY PROGRESS Vol 13, No 2 (2020)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.13.2.2020.31392

Abstract

Ekstrak etanol kayu manis memiliki kandungan fenol yang tinggi yang berpotensi sebagai antioksidan tetapi memiliki bioavailabilitas yang rendah pada kondisi ukuran partikel yang besar. Oleh karena itu, dilakukan analisis karakteristik nanopartikel ekstrak etanol kayu manis. Tujuan penelitian untuk membuat dan mengevaluasi karakteristik nanopartikel ekstrak kayu manis sebagai kandidat antioksidan secara in vitro. Preparasi nanopartikel ekstrak etanol kayu manis dengan metode gelasi ionik. Nanopartikel ekstrak etanol kayu manis memiliki ukuran partikel sebesar 400,3 nm dengan potential zeta +6,60 mV. Spektra Fourier Transform Infrared (FTIR) menunjukkan adanya gugus hidroksil dan gugus amida dari kitosan dan gugus fosfat dari STPP. Studi aktivitas antioksidan secara in vitro dengan metode 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) dan reduksi besi III menunjukkan bahwa nanopartikel ekstrak etanol kayu manis memiliki aktivitas antioksidan yang lebih kecil dibandingkan ekstrak tetapi masih dalam kategori sangat kuat. Aktivitas antioksidan yang diberikan dipengaruhi oleh kandungan total fenolik dan total flavonoid secara berturut-turut sebesar 75,685 ± 1,408 % EAG dan 60,546 ± 0,670 % EK untuk ekstrak etanol kayu manis serta 61,845 ± 0,529 % EAG dan 57,939 ± 0,446 % EK untuk nanopartikel ekstrak etanol kayu manis. Penerapan teknologi nanopartikel pada ekstrak etanol kayu manis melalui ikat silang antara kitosan-TPP dapat mempertahankan aktivitas antioksidannya.ABSTRACT Ethanol extract of cinnamon has a high phenol content which is potential as an antioxidant but has a low bioavailability under conditions of large particle size. Therefore, nanoparticles were prepared for the ethanol extract of cinnamon. The aim of this research was to make and evaluate the characteristics of cinnamon nanoparticles as antioxidant candidates in vitro. The cinnamon nanoparticles were prepared using the ionic gelation method. Cinnamon nanoparticles has a particle size of 400,3 nm with a zeta potential of +6,60 mV. Fourier Transform Infrared (FTIR) spectra showed the presence of a hydroxyl and amida group from chitosan and a phosphate group from STPP. In vitro antioxidant activity studies with the 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) method and ferric reducing activity power showed that cinnamon nanoparticle has smaller antioxidant activity than the extract but is still in the very strong category. The antioxidant activity given is influenced by the total phenolic and total flavonoids content 75.685 ± 1.408% EAG and 60.546 ± 0.670% EK for cinnamon ethanol extract. and 61.845 ± 0.529% EAG and 57.939 ± 0.446% EK for cinnamon ethanol extract nanoparticles, respectively The application of nanoparticle technology to the ethanol extract of cinnamon through chitosan-TPP cross-linking can maintain its antioxidant activity.
EVALUASI SENYAWA BIOAKTIF EKSTRAK KULIT BATANG KAYU TELOR (Alstonia scholaris (L.) R. Br) SEBAGAI KANDIDAT ANTIMALARIA MENGGUNAKAN PENAMBATAN MOLEKULER Hebber, Tri; Fatimawali, Fatimawali; Antasionasti, Irma
PHARMACON Vol 10, No 3 (2021): PHARMACON
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.10.2021.35590

Abstract

ABSTRACTBlackboard tree (Alstonia scholaris (L.) R. Br) is a type of forest wood that is often used by the people of North Sulawesi for the treatment of various diseases including malaria. According to previous in vitro studies, the extract of Blackboard tree bark had a high inhibitory value against Plasmodium falciparum. This study aims to determine the bioactive compounds extracted from Blackboard tree bark and to analyze their potential for the enzymes Dihydroorotat Dehydrogenase, Enoyl Acyl Carrier Protein Reductase and Dihydrofolate Reductase with molecular docking. The dry powder extract of Blackboard tree bark was dissolved using ethanol and chloroform by maceration process then analyzed using GC-MS (Gas Chromatography-Mass Spectrometer) to determine the content of the compounds in the Blackboard tree bark extract. The analysis results obtained that there are probably 9 compounds in the ethanol extract and 60 compounds in the chloroform extract of Blackboard tree barkKeywords : Alstonia scholaris, Antimalaria, GC-MS, Molecular Docking ABSTRAKKayu Telor (Alstonia scholaris (L.) R. Br) merupakan jenis kayu hutan yang sering digunakan masyarakat Sulawesi Utara untuk pengobatan berbagai penyakit termasuk malaria. Menurut penelitian yang dilakukan secara in vitro sebelumnya, ekstrak kulit batang kayu Telor memiliki nilai penghambatan yang tinggi terhadap Plasmodium falciparum. Penelitian ini bertujuan untuk mengetahui senyawa-senyawa bioaktif yang diekstrak dari kulit batang kayu Telor dan menganalisis potensinya terhadap enzim Dihidroorotat Dehidrogenase, Enoyl Acyl Carrier Protein Reductase dan Dihidrofolat Reduktase dengan penambatan molekuler. Ekstrak serbuk kering kulit batang kayu Telor dilarutkan menggunakan etanol dan klorofom dengan proses maserasi kemudian dianalisis menggunakan GC-MS (Gas Cromatography-Mass Spectrometer) untuk mengetahui kandungan senyawa dalam ekstrak kulit batang kayu Telor. Hasil analisis yang diperoleh terdapat kemungkinan 9 senyawa pada ekstrak etanol dan 60 senyawa pada ekstrak kloroform kulit batang kayu Telor.Kata Kunci : Alstonia scholaris, Antimalaria, GC-MS, Penambatan Molekuler
Pelatihan Produksi Minuman Serbuk Jahe, Kunyit, Temulawak Majelis Ta’lim Irsyaadul Ibaad dan PKK Bailang Upaya Peningkatan Produktivitas Ekonomi dan Imunitas Abdullah, Surya Sumantri; Jayanti, Meilani; Jayanto, Imam; Antasionasti, Irma
VIVABIO: Jurnal Pengabdian Multidisiplin Vol 3, No 3 (2021): VIVABIO:Jurnal Pengabdian Multidisiplin
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/vivabio.v3i3.36640

Abstract

Berdasarkan hasil pemeriksaan whole genome sequencing (WGS), virus corona varian Delta telah ditemukan di Sulawesi Utara. Balitbangkes menyatakan Hasil terhadap delapan spesimen adalah positif SARS-CoV-2 Varians of Concern Delta (B.1.617.2). Badan Pusat Statistik Provinsi Sulawesi Utara mencatat sebanyak 287.640 orang di Sulawesi Utara terdampak akibat pandemi Covid-19 atau sekitar 14,80% dari jumlah total penduduk. Sementara itu, jumlah korban pengangguran akibat covid-19 terdampak sebanyak 24.920 orang. Majelis Ta’lim dan PKK Bailang Lingkungan 5 merupakan pranata sosial yang aktif melaksanakan kegiatan sosial dan keagamaan. Pandemi covid-19 belum berakhir sehingga perlu untuk meningkatkan daya tahan tubuh masyarakat dengan memanfaatkan rimpang jahe (Zingiber officinale), kunyit (Curcuma longa), dan temulawak (Curcuma xanthorrhiza). Jahe (Zingiber officinale), kunyit (Curcuma longa), dan temulawak (Curcuma xanthorrhiza) telah terbukti mengandung antioksidan yang akan meningkatkan imunitas tubuh dalam mengatasi varian baru mutasi covid-19. Selain itu juga dilakukan upaya untuk peningkatan produktivitas ekonomi masyarakat dengan pelatihan produksi minuman serbuk dari jahe (Zingiber officinale), kunyit (Curcuma longa), dan temulawak (Curcuma xanthorrhiza) tanpa ampas. Kegiatan ini diharapkan akan meningkatkan imunitas Majelis Ta’lim Irsyaadul Ibaad dan PKK Rayon bailing Lingkungan 5 dan mendorong minat mitra untuk memproduksi minuman serbuk jahe (Zingiber officinale), kunyit (Curcuma longa), dan temulawak (Curcuma xanthorrhiza)  tanpa ampas sebagai upaya peningkatan produktivitas ekonominya. Hasil dari kegiatan ini diharapkan mitra memiliki pengetahuan tentang manfaat jahe (Zingiber officinale), kunyit (Curcuma longa), dan temulawak (Curcuma xanthorrhiza) dan cara pembuatan minuman serbuk instan sehingga meningkatkan wawasan kewirausahaan pada kedua mitra tersebut.
Profil Penetrasi Sediaan Gel Antioksidan Ekstrak Kayu Manis (Cinnamomum burmanii) dengan Variasi Hydrocolloid sebagai Gelling agent Siampa, Jainer Pasca; Wiyono, Weny Indayany; Lestari, Utami Sasmita; Lebang, Julianri Sari; Antasionasti, Irma
Jurnal MIPA Vol 11, No 1 (2022)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jm.v11i1.35787

Abstract

The exposure of sunlight consisting UV radiation has been reported to be the main cause of skin cancer and contribute about 80% of the incidence of skin damage. In an attempt to overcome this problem, antioxidant agents have been used both orally and topically. Compared to oral administration, topical route has been found to be preferred due to the direct action to the desired site. Amongst topical preparation, gel possesses numerous because it provides a cool and fresh sensation. Here, we developed gel antioxidant containing cinnamon extract as active ingredient. Cinnamon was selected as it has a very strong antioxidant activity. This study aimed to obtain the penetration profile of the antioxidant gel of cinnamon extract prepared from two different hydrocolloid agents. The gels were made using various concentrations of Carbopol 940 and NaCMC. The prepared gels were further characterized for their antioxidant capacities using the DPPH free radical scavenging method and their total phenolic contents using the spectrophotometric method. The formulation possessing the strongest antioxidant capacity was continued for ex vivo penetration study using Franz diffusion cells. The results exhibited that the total phenolic value of the cinnamon extract obtained was 908.38 ± 6.54 mg GAE/g extract. The penetration percentages for the formula with Carbopol and NaCMC bases were 53.310 ± 1.217% and 27.969 ± 1.151%, respectively, and the retention percentages were 28.495 ± 0.031% and 18.356 ± 0.191%, respectively. Based on these findings, we concluded that cinnamon extract could potentially be formulated into antioxidant gel preparations and formulas based on Carbopol showed the best penetration profile.
ANALISIS SIFAT FISIKOKIMIA, FARMAKOKINETIK DAN TOKSIKOLOGI PADA PERICARPIUM PALA (Myristica fragransa) SECARA ARTIFICIAL INTELLIGENCE Abdullah, Surya Sumantri; Putra, Purnawan Pontana; Antasionasti, Irma; Rundengan, Gerald; Suoth, Elly Juliana; Abdullah, Rezky Putri Indarwati; Abdullah, Fatamorgana
CHEMISTRY PROGRESS Vol 14, No 2 (2021)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.14.2.2021.37112

Abstract

Pala (Myristica fragransa) mengandung senyawa metabolit sekunder yang menunjukkan aktivitas farmakologi. Namun, bagian perikarpium terutama bagian nonvolatil belum banyak dilaporkan. Tujuan dari penelitian ini sebagai screening awal dengan melihat potensi dari senyawa yang dihasilkan perikarpium pala.Dua belas senyawa digambar dua dimensi, dianalisis menggunakan software dan server prediktor. Software yang digunakan adalah Marvin Sketch, ChembioDraw kemudian dianalisis sifat fisikokimia senyawa tersebut. Selanjutnya, server predictor untuk melihat karakteristik farmakokinetika dan toksisitasnya. Berdasarkan analisis sifat fisikokimia senyawa terbaik yaitu licarin (titik didih, titik kritis temperature dan refraksi molar), guaiacin (titik leleh), dan virolane (titik kritis tekanan). Hasil analisis Lipinski menunjukkan senyawa stigmasterol dan b-sitosterol tidak memenuhi kriteria Lipinski.. Selain itu data farmakokinetika menunjukkan stigmasterol, b-sitosterol, asetoneoglinan memiliki kelarutan dalam air yang rendah. Nilai permeabilitas CaCO-2 dan intestinal absorption semuanya memenuhi. Licarin termasuk substrat P-glikoprotein. Volume Distribusi menunjukkan semua senyawa terikat protein serum. b-sitosterol permeabilitas terhadap sawar darah-otak yang paling baik dan erythro-(7S,8R)-∆8’-7-acetoxy-3,4,3’,5’-tetramethoxy-8-O-4’-neolignan permeabilitasnya buruk. Surinamensin menunjukkan permeabilitas terhadap sistem saraf pusat yang tidak dapat berpenerasi. Elemicin dan surinamensin tidak dimetabolisme oleh enzim sitokrom CYP3A4. B-sitosterol memiliki klirens paling tinggi. Semua senyawa menunjukkan tingkat toksisitas yang rendah untuk penggunaan kulit (kecuali elemicin) dan tidak toksik bagi hati.ABSTRACT Nutmeg (Myristica fragransa) contains secondary metabolites that exhibit pharmacological activity. However, the pericarpium, especially the nonvolatile part, has not been widely reported. The purpose of this study was as an initial screening by looking at the potential compounds produced by the nutmeg pericarp. Twelve compounds were drawn in two dimensions, analyzed using software and predictor servers. The software used is Marvin Sketch, ChembioDraw and then the physicochemical properties of these compounds are analyzed. Furthermore, the predictor server to see the pharmacokinetic characteristics and toxicity. Based on the analysis of the physicochemical properties of the best compounds, compound licarin (boiling point, critical point of temperature and molar refractivity), guaiacin (melting point), and virolane (critical point of pressure). The results of Lipinski's analysis showed that stigmasterol and b-sitosterol compounds did not meet Lipinski's rule. In addition, pharmacokinetic data showed that stigmasterol, b-sitosterol, acetoneoglinan had low solubility in the water. The values of CaCO-2 permeability and intestinal absorption were all satisfactory. Licarin is a P-glycoprotein substrate. The Volume Distribution shows all the compositions of the serum proteins. B-sitosterol with the best permeability to the blood-brain barrier and erythro-(7S,8R)-∆8'-7-acetoxy-3,4,3',5'-tetramethoxy-8-O-4'-neolignan bad permeability. Surinamensin exhibits permeability to the non-permeable central nervous system. Elemicin and surinamensin are not metabolized by the cytochrome enzyme CYP3A4. B-sitosterol has the highest clearance. All compounds show a low level of toxicity for skin use (except elemicin) and are not toxic to the liver. 
Uji Aktivitas Antioksidan dan Karakterisasi Minyak Tulang Ikan Tindarung : Antioxidant Activity and Characterization of Fish Oil from Marlin Fish Bone Antasionasti, Irma; ABDULLAH, SURYA SUMANTRI; Siampa, Jainer Pasca; Jayanto, Imam
Media Teknologi Hasil Perikanan Vol 12 No.1 (2024)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/mthp.12.1.2024.54368

Abstract

Marlin fish (tindarung) is one of the fish produced in the waters of North Sulawesi. The process of processing tindarung fish produces fish bone waste which can be maximized for processing as fish oil. Fish oil contains essential fatty acids that the body cannot produce naturally. This research aimed to determine the antioxidant activity and characteristics of tindarung fish bone oil. Tindarung fish bone oil extraction was carried out using wet rendering method. Next, antioxidant activity was tested using DPPH method. Characterization was carried out using the titration method. Based on the results of the antioxidant activity test, tindarung fish bone oil has an antioxidant activity of 20,958 ± 2,736 µg/mL. Apart from that, tindarung fish bone oil has characteristics that meet the standards of the International Fish Meal and Oil Manufacturers Association, namely a density of 0.91 g/mL; peroxide value of 20 meq/kg; free fatty acids of 5.65%; and the iodine number is 7.25 mg/100 g. This shows that tindarung fish bone oil can be consumed regularly as a supplement to maintain health. Keywords: antioxidant activity, characterization, fish oil, marlin fish Ikan Tindarung merupakan salah satu ikan yang dihasilkan di perairan Sulawesi Utara. Proses pengolahan ikan tindarung menghasilkan limbah tulang ikan yang dapat dimaksimalkan pengolahannya sebagai minyak ikan. Minyak ikan mengandung asam lemak esensial yang tidak dapat diproduksi secara alami oleh tubuh. Tujuan penelitian ini adalah untuk menentukan aktivitas antioksidan dan karakteristik dari minyak tulang ikan tindarung. Ekstraksi minyak tulang ikan tindarung dilakukan dengan menggunakan metode wet rendering. Selanjutnya, pengujian aktivitas antioksidan menggunakan metode DPPH. karakterisasi dilakukan menggunakan metode titrasi. Berdasarkan hasil ujiaaktivitasantioksidan, minyak tulang ikan tindarung memiliki aktivitas antioksidan sebesar 20,958 ± 2,736 µg/mL.  selain itu, minyak tulang ikan tindarung memiliki karakteristik yang memenuhi standar International Fish Meal and Oil Manufacturers Association, yaitu massa jenis sebesar 0,91 g/mL; bilangan peroksida sebesar 20 meq/kg; asam lemak bebas sebesar 5,65%; dan bilangan iod sebesar 7,25 mg/100 g. Hal ini menunjukkan bahwa minyak tulang ikan tindarung dapat dikonsumsi secara teratur sebagai suplemen untuk menjaga kesehatan. Kata kunci: aktivitas antioksidan, karakterisasi, minyak ikan, tindarung
Studi In Silico Senyawa Aktif Minyak Ikan Tindarung Sebagai Penurun Kolesterol Melalui Inhibitor HMG-KoA RUMALUTUR, CHRISTHALIA IEWANDA; PANI, PUTRI MARGARETHA GLAUDY; PRAYOGA, DESHANDA KURNIAWAN; LAPIAN, ALDA JULISTY GABRIELA; GONI, BRIGITA CHRISTANIA AURORA; FATIMAWALI; ANTASIONASTI, IRMA
Jurnal Farmasi Medica/Pharmacy Medical Journal (PMJ) Vol. 7 No. 1 (2024): Volume 7, No 1, Tahun 2024
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pmj.v7i1.46260

Abstract

Tindarung fish oil contains omega-3 fatty acids, namely DHA (Docosahexaenoic acid) and EPA (Eicosapentaenoic acid). Therefore, tindarung fish oil has the potential to be developed as a cholesterol drug. This study aims to determine the activity of HMG-CoA reductase from fatty acids of tindarung fish oil using an in silico study with molecular docking. The receptor used was the enzyme HMG-CoA reductase (PDB ID: 1HW9) with the native ligand simvastatin prepared using YASARA. The fatty acid compounds were drawn using MarvinSketch at pH 7.4 with 10 confirmations. In the molecular anchoring process, the PLANTS program was used and visualized in 2D with Discovery Studio Visualizer and in 3D with Pymol. The native ligand showed a docking value of -76.4052. Compounds 1,2,3,8,11, and 12 had relative values for simvastatin of more than 95%. These compounds have molecular anchoring activity similar to simvastatin so it is predicted to be able to reduce cholesterol levels by inhibiting the HMG-CoA reductase enzyme.
Co-Authors AA Sudharmawan, AA Abdulgani, Nayla Inayah Abdullah, Fatamorgana Abdullah, Rezky Putri Indarwati Abdullah, Surya Sumantri Adithya Yudistira Bawole, Avinda Shania Wiane Dadiani, Ni Made Damanis, Frelinsia V.M. Defny S. Wewengkang, Defny S. Dolongtelide, Jeclin Inebel Dzaki, Najwan Nafis Edwin de Queljoe Elly Suoth Ering, Mariando N. Fatimawali . GONI, BRIGITA CHRISTANIA AURORA Hariyanto, Yuanita Amalia Hebber, Tri Herny E.I. Simbala Jayanto, Imam Julianri Sari Lebang, Julianri Sari Kalalo, Marko Jeremia LAPIAN, ALDA JULISTY GABRIELA Lasut, Misella R. C. Lasut, Misella Regina Lestari, Utami Sasmita Ma'ruf, Nurul Qalbiyyah Mahendra Kusuma Nugraha Maringka, Pingkan C. Meilani Jayanti Muhammad Setiawan Nurhamidin, Anastasia P.R. Nurmiati Nurmiati PANI, PUTRI MARGARETHA GLAUDY Paulina yamlean Prayoga, Deshanda Kurniawan Rahmawati, Dian Wahyu Ratte, Titah Amelia Rumagit, Tjandra A. RUMALUTUR, CHRISTHALIA IEWANDA Rundengan, Gerald Rundengan, Gerald Edward Samiun, Asriyani Siampa, Jainer Pasca Sri Sudewi, Sri Sukmaningrum, Krisnawati Sumantri Abdullah, Surya Sutrisna, Agung Takawaian, Agrita Feisilia Tasiam, Ezrani TRINA EKAWATI TALLEI Trina Tallei Widya Astuty Lolo, Widya Astuty Wiyono, Weny I. Wiyono, Weny Indayany